LOCUS       Exported                8331 bp ds-DNA    circular SYN 20-9ÔÂ-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    enDelIscB-T5E
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8331)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported 2025Äê9ÔÂ20ÈÕ from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8331
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        295..572
                     /note="chicken beta-actin promoter"
     misc_feature    573..801
                     /note="Hyrid Intron"
     misc_feature    807..812
                     /note="Feature 22"
     misc_feature    813..818
                     /note="Kozak sequence"
     misc_feature    819..821
                     /note="Kozak"
     CDS             822..2189
                     /codon_start=1
                     /note="enDelIscB"
                     /translation="SRVLVVDADRRPLAPCTPRRARLLLNSGKAAVLRRYPFTIILKQS
                     YPTASPRPVRLKLDPGSKTTGIAVVTEATGEVVWAAELQHRGQLIKNALEKRRALRRSR
                     RNRKTRYRPARFLNRKRTGPPLLSSADTVSTLGKWLPPSLQHRIEVIMTWVHRLRRYLP
                     ITAISQEIVRFDMQKMQNPEISGVEYQQGTLFGYEVREYLLDKWRRQCGYCGAKDKRLE
                     VDHIVPRSHGGSDRVSNLTLSCEPCNKRKNQRPAAVFLAKKPEVLQKLQRQAKAPLKDA
                     AAVNSTRYALLERLKATGLPVEVASGGRTKFNRSRRQIPKTHWLDAACVGASTPEVLKW
                     EAVRPLAIKAMGHGKRQVVNVDAYGFPRGKAKGIPVHPFRTGDIVRAEIPKGKYKGTYV
                     SRIAETTTSKPLAGFKSKTGKRIQCHTKHMTKLFNRDGYGYGFLKAPEPRQTVISES"
     variation       852..854
                     /note="C12R"
     variation       1107..1109
                     /note="S97K"
     variation       1116..1118
                     /note="S100A"
     variation       1128..1130
                     /note="G104S"
     variation       1167..1169
                     /note="W117F"
     misc_feature    1242..1244
                     /note="A142P"
     variation       1767..1769
                     /note="E317R"
     variation       1836..1838
                     /note="Q340K"
     variation       2004..2006
                     /note="V396K"
     misc_feature    2124..2126
                     /note="S436R"
     CDS             2190..2191
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /note="nucleoplasmin NLS"
                     /translation=""
     misc_feature    2196..2294
                     /note="Linker"
     misc_feature    2196..2274
                     /note="hecheng"
     misc_feature    2295..3167
                     /note="T5EXO"
     misc_feature    2295..3167
                     /note="hecheng"
     CDS             3168..3215
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /note="nucleoplasmin NLS"
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    3224..3247
                     /note="i16-2"
     polyA_signal    3249..3424
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    3425..3707
                     /note="F3"
     promoter        3457..3706
                     /label="U6 Promoter"
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    3707
                     /note="Bsa1"
     misc_feature    3707
                     /note="replace"
     misc_feature    3708..3990
                     /note="F4"
     misc_feature    3708..3728
                     /note="Bsa1"
     misc_feature    3708..3726
                     /note="replace"
     misc_feature    3729..3976
                     /note="Delta-35-41+GAAA+T205A+Delta-221-240+GAAA"
     misc_feature    3729..3747
                     /note="DR"
     misc_feature    3748..3751
                     /note="LOOP"
     misc_feature    3752..3782
                     /note="anti-repeat"
     misc_feature    3930
                     /note="T205A"
     misc_feature    3991..3996
                     /note="Feature 21"
     enhancer        4057..4360
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4361..4564
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             4593..5303
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             5024
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation=""
     rep_origin      5858..6313
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6595..6699
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             6700..7560
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7731..8319
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 aggtacccgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
       61 gcccattgac gtcaatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      121 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      181 ctattgacgt caatgacggt aaatggcccg cctggcattg tgcccagtac atgaccttat
      241 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg
      301 tgagccccac gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt
      361 atttatttat tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg
      421 cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg
      481 cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc
      541 ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgacgctg ccttcgcccc
      601 gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
      661 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagct gagcaagagg
      721 taagggttta agggatggtt ggttggtggg gtattaatgt ttaattacct ggagcacctg
      781 cctgaaatca ctttttttca ggttggaccg gtgccaccat gagcagagtg ctggtggtgg
      841 atgctgatag gcgccctctg gccccttgca cccctcggag agccagactg ctgctgaatt
      901 ctggcaaagc tgccgtgctg agacggtacc ctttcaccat catcctgaag cagagctacc
      961 ccaccgccag ccctagaccc gtgcggctga agctggaccc cggaagcaag accaccggca
     1021 tcgccgtggt gacagaggcc acaggagaag tggtgtgggc cgctgagctg cagcacagag
     1081 gccagctgat caagaacgcc ctggaaaaga gaagagccct gcggcgcagc cggcgaaacc
     1141 ggaagacacg gtatagacct gccagattcc tgaatagaaa gagaacagga cctccactgc
     1201 tgagcagcgc cgacactgtg tctaccctgg gcaagtggtt gcccccgagc ctgcagcata
     1261 ggatcgaggt gatcatgacc tgggtccaca gactgagaag atacctgccc atcaccgcca
     1321 tttctcagga gatcgtgaga ttcgacatgc aaaagatgca gaaccccgaa atcagcggcg
     1381 tggaatacca gcagggcacc ctcttcggct acgaggtgcg ggaatacctc ctggacaagt
     1441 ggcggagaca atgtggctac tgcggcgcta aggacaagag acttgaggtc gaccacatcg
     1501 ttcctcggag ccacggcggc agcgacagag tgtccaacct gacactgtcc tgcgagcctt
     1561 gcaacaaaag aaagaaccag aggccagctg ctgtgttcct ggctaaaaag cccgaggtcc
     1621 tgcagaagct gcagcggcag gccaaggccc ctctgaagga cgccgccgcc gtgaacagca
     1681 cccgttacgc cctgctggaa agactgaagg ccaccggcct gcctgtggaa gtggccagcg
     1741 gcggaagaac caagttcaat agaagcagga gacagatccc caagacccac tggctggatg
     1801 ccgcctgcgt gggcgcttct acacctgagg tgctgaagtg ggaggccgtt agaccactgg
     1861 ccatcaaggc catgggccac ggcaaacggc aggtggtcaa cgtggacgcc tacggatttc
     1921 ctcggggcaa ggccaaaggc atccctgtgc accccttcag aaccggagat atcgtgcggg
     1981 ccgagattcc aaagggcaaa tacaagggaa catacgtgtc cagaatcgcc gagacaacca
     2041 catctaagcc tctagcaggc tttaagagca agaccggcaa gcggatccag tgtcacacca
     2101 agcacatgac aaagctgttc aaccgcgacg gctatggcta cggcttcctg aaagcccctg
     2161 aacctagaca gaccgtgatc agcgagtcta agctttccgg aggatctagc ggaggctcct
     2221 ctggcagcga gacaccagga acaagcgagt cagcaacacc agagagcagt ggcggcagca
     2281 gcggcggcag cagtatgagc aagtcctggg gcaagttcat cgaggaagag gaagctgaaa
     2341 tggcctctag acgcaacctg atgatcgtgg atggcaccaa cctcggcttc aggttcaagc
     2401 acaacaactc caagaaacct ttcgccagca gctacgtgtc taccatccag agcctggcca
     2461 agagctatag cgccagaacc acaatcgtgc tcggcgataa gggcaaaagc gtgttcagac
     2521 tggaacatct gcctgagtac aagggcaacc gggacgagaa gtacgcccag cggaccgagg
     2581 aagagaaggc tctggatgag cagtttttcg agtacctgaa agacgccttc gagctttgta
     2641 aaacaacatt tcctacattc acaatcagag gagtggaagc cgatgatatg gccgcttata
     2701 tcgtgaagct gatcggccac ctgtacgacc acgtgtggct gatctctaca gatggtgact
     2761 gggacaccct gctgaccgac aaggtgtcca gattcagctt caccaccaga agagagtacc
     2821 acctgagaga tatgtacgag caccacaatg tggacgacgt ggagcaattt atcagcctga
     2881 aggccattat gggcgacctg ggcgacaaca tccggggcgt tgaaggcatc ggcgctaaaa
     2941 gaggctacaa catcatccgg gagttcggca atgtgctgga catcatcgac cagctgcccc
     3001 tgccaggcaa gcagaaatac atccagaacc tgaacgccag cgaggagctg ctgttcagaa
     3061 atctgattct ggtggacctg cctacctact gcgtggatgc catcgccgcc gtgggccagg
     3121 acgtgctgga caagtttacc aaggacatcc tggaaatcgc cgagcagaaa aggccggcgg
     3181 ccacgaagaa ggccggccag gcaaagaaga aaaagtaaga attcctagag ctcgctgatc
     3241 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     3301 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     3361 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     3421 ggaggattgg gaagagaata gcaggcatgc tggggagagg gcctatttcc catgattcct
     3481 tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta
     3541 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
     3601 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
     3661 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgggga gaccgagaga
     3721 gggtctcagt tgtagcctcc actcattgaa aaatgagtgc gggaaaccgc agctacaacc
     3781 agactaagtt ctttgggaac tacgttattg tcgtcatcat acccacgggt gcgtgccagc
     3841 ctgtggctct atggtcgtgc attaaacagg gactttggag ggtgcccagt gtgtacggcg
     3901 tcaaaagcgg cgataacatt gtcgaggcaa actttacgtg acggcgaaag cccgatcgct
     3961 agctttaagg ctaatttttt atttttttgc ggccgctagt tattaatagt aatcaattac
     4021 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     4081 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     4141 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     4201 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     4261 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     4321 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     4381 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     4441 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     4501 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     4561 agctggttta gtgaaccgtc agatccgcta gcatggtgag caagggcgag gaggataaca
     4621 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
     4681 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
     4741 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
     4801 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
     4861 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
     4921 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
     4981 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaaaaccatg ggctgggagg
     5041 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
     5101 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
     5161 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
     5221 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
     5281 gcatggacga gctgtacaag taaggatccg ggatgcagaa attgatgatc tattaaacaa
     5341 taaagatgtc cactaaaatg gaagtttttc ctgtcatact ttgttaagaa gggtgagaac
     5401 agagtaccta cattttgaat ggaaggattg gagctacggg ggtgggggtg gggtgggatt
     5461 agataaatgc ctgctcttta ctgaaggctc tttactattg ctttatgata atgtttcata
     5521 gttggatatc ataatttaaa caagcaaaac caaattaagg gccagctcat tcctcccact
     5581 catgatctat agatctatag atctctcgtg ggatcattgt ttttctcttg attcccactt
     5641 tgtggttcta agtactgtgg tttccaaatg tgtcagtttc atagcctgaa gaacgagatc
     5701 agcagcctct gttccacata cacttcattc tcagtattgt tttgccaagt tctaattcca
     5761 tcagaagctg gtcgacctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     5821 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     5881 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5941 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     6001 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     6061 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     6121 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     6181 acactcaacc ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc
     6241 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     6301 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     6361 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     6421 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     6481 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     6541 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     6601 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     6661 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     6721 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     6781 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     6841 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     6901 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     6961 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     7021 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     7081 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     7141 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7201 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     7261 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7321 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7381 tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat tgcagcactg
     7441 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     7501 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     7561 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     7621 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     7681 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7741 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7801 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7861 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     7921 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7981 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8041 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     8101 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8161 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8221 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8281 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa c
//
