LOCUS       Exported                8561 bp ds-DNA    circular SYN 20-9ÔÂ-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    IABE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8561)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported 2025Äê9ÔÂ20ÈÕ from SnapGene 2.3.2
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8561
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      30..618
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    638..923
                     /note="CMV enhancer"
     misc_feature    717
                     /note="A-to-G R2M4"
     promoter        925..1202
                     /note="chicken beta-actin promoter"
     intron          1203..1431
                     /note="hybrid intron"
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     misc_feature    1421..1431
                     /note="Hyrid Intron"
     CDS             1449..4070
                     /codon_start=1
                     /note="Feature 40"
                     /translation="MSRVLVVDADRRPLAPCTPRRARLLLNSGKAAVLRRYPFTIILKQ
                     SYPTASPRPVRLKLAPGSKTTGIAVVTEATGEVVWAAELQHRGQLIKNALEKRRALRRS
                     RRNRKTRYRPARFLNRKRTGPPLLSSADTVSTLGKWLPPSLQHRIEVIMTWVHRLRRYL
                     PITAISQEIVRFDMQKMQNPEISGVEYQQGTLFGYEVREYLLDKWRRQCGYCGAKDKRL
                     EVDHIVPRSHGGSDRVSNLTLSCEPCNKRKNQRPAAVFLAKKPEVLQKLQRQAKAPLKD
                     AAAVNSTRYALLERLKATGLPVEVASGGRTKFNRSRRQIPKTHWLDAACVGASTPEVLK
                     WEAVRPLAIKAMGHGKRQVVNVDAYGFPRGKAKGIPVHPFRTGDIVRAEIPKGKYKGTY
                     VSRIAETTTSKPLAGFKSKTGKRIQCHTKHMTKLFNRDGYGYGFLKAPEPRQTVISESS
                     GGSSGGSSGSETPGTSESATPESSGGSSGGSSEVEFSHEYWMRHALTLAKRARDEREVP
                     VGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCV
                     MCAGAMIHSRIGRVVFGWRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCD
                     FYRMPRQVFNAQKKAQSSINSGGSSGGSKRTADGSEFESPKKKRKVSGGSSGGSSEVEF
                     SHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQ
                     GGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGWRNSKRGAAGSLMNVLNYP
                     GMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQSSINKRTADGSEFESPKKKR
                     KV"
     misc_structure  1449..1451
                     /note="PLMP domain"
     CDS             1449..1451
                     /codon_start=1
                     /note="OgeuIscB"
                     /translation="M"
     CDS             1452..2819
                     /codon_start=1
                     /note="enDelIscB(D60A)"
                     /translation="SRVLVVDADRRPLAPCTPRRARLLLNSGKAAVLRRYPFTIILKQS
                     YPTASPRPVRLKLAPGSKTTGIAVVTEATGEVVWAAELQHRGQLIKNALEKRRALRRSR
                     RNRKTRYRPARFLNRKRTGPPLLSSADTVSTLGKWLPPSLQHRIEVIMTWVHRLRRYLP
                     ITAISQEIVRFDMQKMQNPEISGVEYQQGTLFGYEVREYLLDKWRRQCGYCGAKDKRLE
                     VDHIVPRSHGGSDRVSNLTLSCEPCNKRKNQRPAAVFLAKKPEVLQKLQRQAKAPLKDA
                     AAVNSTRYALLERLKATGLPVEVASGGRTKFNRSRRQIPKTHWLDAACVGASTPEVLKW
                     EAVRPLAIKAMGHGKRQVVNVDAYGFPRGKAKGIPVHPFRTGDIVRAEIPKGKYKGTYV
                     SRIAETTTSKPLAGFKSKTGKRIQCHTKHMTKLFNRDGYGYGFLKAPEPRQTVISES"
     variation       1482..1484
                     /note="C12R"
     variation       1626..1628
                     /note="D60A"
     variation       1737..1739
                     /note="S97K"
     variation       1746..1748
                     /note="S100A"
     variation       1758..1760
                     /note="G104S"
     variation       1797..1799
                     /note="W117F"
     misc_feature    1872..1874
                     /note="A142P"
     variation       2397..2399
                     /note="E317R"
     variation       2466..2468
                     /note="Q340K"
     variation       2634..2636
                     /note="V396K"
     variation       2754..2756
                     /note="S436R"
     CDS             2820..2915
                     /codon_start=1
                     /note="LINKER"
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             2820..2915
                     /codon_start=1
                     /note="Feature 40"
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             2916..3413
                     /codon_start=1
                     /note="Feature 40"
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
                     RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFG
                     WRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQS
                     SIN"
     CDS             2916..3413
                     /codon_start=1
                     /note="TAD8e (V106W)"
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
                     RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFG
                     WRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQS
                     SIN"
     misc_feature    3228..3230
                     /note="V106W"
     CDS             3438..3491
                     /codon_start=1
                     /note="bpSV40 NLS"
                     /translation="KRTADGSEFESPKKKRKV"
     CDS             3516..4013
                     /codon_start=1
                     /note="TAD8e (V106W)"
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
                     RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFG
                     WRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQS
                     SIN"
     misc_feature    3828..3830
                     /note="V106W"
     CDS             4014..4067
                     /codon_start=1
                     /note="bpNLS"
                     /translation="KRTADGSEFESPKKKRKV"
     misc_feature    4095..4099
                     /note="i16-2"
     polyA_signal    4101..4276
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4277..4559
                     /note="F3"
     promoter        4309..4558
                     /label="U6 Promoter"
                     /note="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    4559
                     /note="Bsa1"
     misc_feature    4559
                     /note="replace"
     misc_feature    4560..4842
                     /note="F4"
     misc_feature    4560..4580
                     /note="Bsa1"
     misc_feature    4560..4578
                     /note="replace"
     misc_feature    4581..4828
                     /note="Delta-35-41+GAAA+T205A+Delta-221-240+GAAA"
     misc_feature    4581..4599
                     /note="DR"
     misc_feature    4600..4603
                     /note="LOOP"
     misc_feature    4604..4634
                     /note="anti-repeat"
     misc_feature    4782
                     /note="T205A"
     enhancer        4909..5212
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5213..5416
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    5445..5453
                     /note="Kozak"
     CDS             5451..6161
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /note="mCherry"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     DDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     rep_origin      6718..7173
                     /direction=RIGHT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7455..7559
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             7560..8420
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
       61 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
      121 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
      181 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
      241 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
      301 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
      361 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
      421 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
      481 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
      541 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
      601 gggcggagcc tatggaaaaa cgccagcaac aggtacccgt tacataactt acggtaaatg
      661 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaatagta acgccaatag
      721 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      781 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      841 cctggcattg tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      901 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      961 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     1021 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
     1081 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     1141 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     1201 cgggagtcgc tgcgacgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
     1261 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     1321 cctccgggct gtaattagct gagcaagagg taagggttta agggatggtt ggttggtggg
     1381 gtattaatgt ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggaccg
     1441 gtgccaccat gagcagagtg ctggtggtgg atgctgatag gcgccctctg gccccttgca
     1501 cccctcggag agccagactg ctgctgaatt ctggcaaagc tgccgtgctg agacggtacc
     1561 ctttcaccat catcctgaag cagagctacc ccaccgccag ccctagaccc gtgcggctga
     1621 agctggcccc cggaagcaag accaccggca tcgccgtggt gacagaggcc acaggagaag
     1681 tggtgtgggc cgctgagctg cagcacagag gccagctgat caagaacgcc ctggaaaaga
     1741 gaagagccct gcggcgcagc cggcgaaacc ggaagacacg gtatagacct gccagattcc
     1801 tgaatagaaa gagaacagga cctccactgc tgagcagcgc cgacactgtg tctaccctgg
     1861 gcaagtggtt gcccccgagc ctgcagcata ggatcgaggt gatcatgacc tgggtccaca
     1921 gactgagaag atacctgccc atcaccgcca tttctcagga gatcgtgaga ttcgacatgc
     1981 aaaagatgca gaaccccgaa atcagcggcg tggaatacca gcagggcacc ctcttcggct
     2041 acgaggtgcg ggaatacctc ctggacaagt ggcggagaca atgtggctac tgcggcgcta
     2101 aggacaagag acttgaggtc gaccacatcg ttcctcggag ccacggcggc agcgacagag
     2161 tgtccaacct gacactgtcc tgcgagcctt gcaacaaaag aaagaaccag aggccagctg
     2221 ctgtgttcct ggctaaaaag cccgaggtcc tgcagaagct gcagcggcag gccaaggccc
     2281 ctctgaagga cgccgccgcc gtgaacagca cccgttacgc cctgctggaa agactgaagg
     2341 ccaccggcct gcctgtggaa gtggccagcg gcggaagaac caagttcaat agaagcagga
     2401 gacagatccc caagacccac tggctggatg ccgcctgcgt gggcgcttct acacctgagg
     2461 tgctgaagtg ggaggccgtt agaccactgg ccatcaaggc catgggccac ggcaaacggc
     2521 aggtggtcaa cgtggacgcc tacggatttc ctcggggcaa ggccaaaggc atccctgtgc
     2581 accccttcag aaccggagat atcgtgcggg ccgagattcc aaagggcaaa tacaagggaa
     2641 catacgtgtc cagaatcgcc gagacaacca catctaagcc tctagcaggc tttaagagca
     2701 agaccggcaa gcggatccag tgtcacacca agcacatgac aaagctgttc aaccgcgacg
     2761 gctatggcta cggcttcctg aaagcccctg aacctagaca gaccgtgatc agcgagtctt
     2821 ctggaggatc tagcggaggc tcctctggca gcgagacacc aggaacaagc gagtcagcaa
     2881 caccagagag cagtggcggc agcagcggcg gcagctctga ggtggagttt tcccacgagt
     2941 actggatgag acatgccctg accctggcca agagggcacg ggatgagagg gaggtgcctg
     3001 tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac agagccatcg
     3061 gcctacacga cccaacagcc catgccgaaa ttatggccct gagacaaggc ggcctggtca
     3121 tgcagaacta cagactgatt gacgccaccc tgtacgtgac attcgagcct tgcgtgatgt
     3181 gcgctggcgc catgatccac tctaggatcg gccgcgtggt gtttggatgg agaaattcta
     3241 aaagaggcgc cgcaggctcc ctgatgaacg tgctgaacta ccccggcatg aatcaccgcg
     3301 tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc gacttctatc
     3361 ggatgcctag acaggtgttc aatgctcaga agaaggccca gagttccatc aactctggag
     3421 gatctagcgg aggatccaag agaaccgctg atggctcaga gtttgagagc ccaaaaaaga
     3481 aaagaaaagt cagtggcggc agcagcggcg gcagctctga ggtggagttt tcccacgagt
     3541 actggatgag acatgccctg accctggcca agagggcacg ggatgagagg gaggtgcctg
     3601 tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac agagccatcg
     3661 gcctacacga cccaacagcc catgccgaaa ttatggccct gagacaaggc ggcctggtca
     3721 tgcagaacta cagactgatt gacgccaccc tgtacgtgac attcgagcct tgcgtgatgt
     3781 gcgctggcgc catgatccac tctaggatcg gccgcgtggt gtttggatgg agaaattcta
     3841 aaagaggcgc cgcaggctcc ctgatgaacg tgctgaacta ccccggcatg aatcaccgcg
     3901 tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc gacttctatc
     3961 ggatgcctag acaggtgttc aatgctcaga agaaggccca gagttccatc aacaagagaa
     4021 ccgccgacgg ctccgagttc gagagcccca agaagaagcg caaagtgtga gaattcctag
     4081 agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4141 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4201 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4261 ggacagcaag ggggaggatt gggaagagaa tagcaggcat gctggggaga gggcctattt
     4321 cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt
     4381 aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt
     4441 cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta
     4501 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccggg
     4561 gagaccgaga gagggtctca gttgtagcct ccactcattg aaaaatgagt gcgggaaacc
     4621 gcagctacaa ccagactaag ttctttggga actacgttat tgtcgtcatc atacccacgg
     4681 gtgcgtgcca gcctgtggct ctatggtcgt gcattaaaca gggactttgg agggtgccca
     4741 gtgtgtacgg cgtcaaaagc ggcgataaca ttgtcgaggc aaactttacg tgacggcgaa
     4801 agcccgatcg ctagctttaa ggctaatttt ttattttttt gcggccgcta gttattaata
     4861 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     4921 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     4981 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     5041 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     5101 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     5161 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     5221 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     5281 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     5341 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     5401 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgccacc atggtgagca
     5461 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg
     5521 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     5581 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg
     5641 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca
     5701 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     5761 tcgatgacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca
     5821 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     5881 aaaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg
     5941 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     6001 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     6061 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     6121 gccgccactc caccggcggc atggacgagc tgtataagtg atgtacaccg ggatgcagaa
     6181 attgatgatc tattaaacaa taaagatgtc cactaaaatg gaagtttttc ctgtcatact
     6241 ttgttaagaa gggtgagaac agagtaccta cattttgaat ggaaggattg gagctacggg
     6301 ggtgggggtg gggtgggatt agataaatgc ctgctcttta ctgaaggctc tttactattg
     6361 ctttatgata atgtttcata gttggatatc ataatttaaa caagcaaaac caaattaagg
     6421 gccagctcat tcctcccact catgatctat agatctatag atctctcgtg ggatcattgt
     6481 ttttctcttg attcccactt tgtggttcta agtactgtgg tttccaaatg tgtcagtttc
     6541 atagcctgaa gaacgagatc agcagcctct gttccacata cacttcattc tcagtattgt
     6601 tttgccaagt tctaattcca tcagaagctg gtcgacctgc aggggcgcct gatgcggtat
     6661 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
     6721 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6781 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6841 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6901 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
     6961 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7021 tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt gatttataag
     7081 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7141 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
     7201 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     7261 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     7321 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     7381 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     7441 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     7501 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     7561 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     7621 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     7681 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     7741 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     7801 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     7861 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     7921 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgattg
     7981 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8041 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8101 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8161 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8221 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggaagcc
     8281 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8341 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     8401 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     8461 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     8521 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc a
//
