LOCUS       plasmid                 8106 bp    DNA     circular SYN 30-JUN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     intron          complement(4685..5623)
                     /note="pLannotate"
                     /label="EF-1α intron A"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             complement(1943..2659)
                     /note="pLannotate"
                     /label="EGFP"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(3593..4270)
                     /note="pLannotate"
                     /label="mRFP1"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    complement(1588..1812)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(join(8082..8106,1..97))
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        5907..6011
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(227..1249)
                     /note="pLannotate"
                     /label="HygR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.7"
                     /fragment="False"
                     /other="CDS"
     CDS             6012..6872
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      7043..7631
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    complement(1257..1304)
                     /note="pLannotate"
                     /label="FRT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        7955..7985
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    7919..7940
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(5624..5834)
                     /note="pLannotate"
                     /label="EF-1α core promoter"
                     /database="snapgene"
                     /identity="99.1"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(4271..4291)
                     /note="pLannotate"
                     /label="attB3"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(4292..4669)
                     /note="pLannotate"
                     /label="H2BC12"
                     /database="swissprot"
                     /identity="99.2"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     protein_bind    7993..8009
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      complement(1309..1542)
                     /note="pLannotate"
                     /label="f1 ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="65.2"
                     /fragment="True"
                     /other="rep_origin"
     misc_feature    complement(1883..1942)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(92..217)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="97.6"
                     /match_length="33.3"
                     /fragment="True"
                     /other="CDS"
     CDS             7814..7906
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(1563..1584)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
       61 tttatttgta accattataa gctgcaataa acaagttggg gtgggcgaag aactccagca
      121 tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca
      181 acctttcata gaaggcggcg gtggaatcga aatctcgtag tacgtgctat tcctttgccc
      241 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
      301 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
      361 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
      421 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc
      481 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
      541 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
      601 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
      661 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
      721 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
      781 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
      841 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg
      901 cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
      961 caccctgtgc acggcgggag atgcaatagg tcaggctctc gctgaattcc ccaatgtcaa
     1021 gcacttccgg aatcgggagc gcggccgatg caaagtgccg ataaacataa cgatctttgt
     1081 agaaaccatc ggcgcagcta tttacccgca ggacatatcc acgccctcct acatcgaagc
     1141 tgaaagcacg agattcttcg ccctccgaga gctgcatcag gtcggagacg ctgtcgaact
     1201 tttcgatcag aaacttctcg acagacgtcg cggtgagttc aggctttttg gccaaggaag
     1261 ttcctatact ttctagagaa taggaacttc ggaataggaa cttctaggta cgtgaaccat
     1321 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     1381 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     1441 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     1501 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtggggatac cccctagagc
     1561 cccagctggt tctttccgcc tcagaagcca tagagcccac cgcatcccca gcatgcctgc
     1621 tattgtcttc ccaatcctcc cccttgctgt cctgccccac cccacccccc agaatagaat
     1681 gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg acagtgggag
     1741 tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat ggctggcaac
     1801 tagaaggcac agtcgaggct gatcagcggg tttaaacggg ccctctagac tcgagcggcc
     1861 gccactgtgc tggatccggt agcgggcccg cggtaccgtc gactgcagaa ttcgaagctt
     1921 gagctcgaga tctgagtccg gacttgtaca gctcgtccat gccgagagtg atcccggcgg
     1981 cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgctcaggg
     2041 cggactgggt gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg
     2101 tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct
     2161 tgaagttcac cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt
     2221 tgtagttgta ctccagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct
     2281 tcagctcgat gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt
     2341 agttgccgtc gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg
     2401 acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt
     2461 aggtcagggt ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga
     2521 acttcagggt cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact
     2581 tgtggccgtt tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct
     2641 cctcgccctt gctcaccatg gtggcgaccg gatctgcaga attccaccac actggactag
     2701 tggatccgag ctcggtacca agcttcggct tattattccg cggcggccgc agcggcagct
     2761 acaacaaccg cgtcgctctc cgctcaattt ccaagagcca gctttgaagc caagtgcgag
     2821 cagtttcaaa ctcaccgcgc taaagggccc cggattcacc aatcgggtag cccgtagact
     2881 ttcaaagcag ccaatcagag cccagctacg ctgggcaggc cttcccgggt ggctagagcg
     2941 cgaaagaaag aggaaagggc ggctagagaa aaagcaggag ggcgggcgcc aactgagtgc
     3001 gagcgcaagc gctctcctcc agtcgggaga gtgtcgtcct actgtttcta gtcagcggag
     3061 caggaagcta ctgttcgctc cgttcttctt ttaaattttt tctcccagca ttggcacagt
     3121 tcaaatttat tatactcaaa atagctcatc aaaaaagtga tattgtgttt acatcgagat
     3181 tccattactt tcactcctaa tacttagggt taggagtgta tagttatgtt tttctaaatg
     3241 cgtgatttgc gggctggctc caaggagcac atttcagtga ccttaagaag gaaatggaaa
     3301 actcaaaaga ccgcctcaaa aatgtaaagg aaaatttatt atttatatcg ctgtgctttg
     3361 tttctacctc atttttgaat ttaatattaa attattttat tatttacatt ttgtttatta
     3421 tacaattaaa aacatttgaa atgtattaaa ttttaaaata ttttcacatc agaattttaa
     3481 atatatagag agaggcgtgc atttagagac tgggtctcat tatgttgcgc atgctggcct
     3541 ccaaatcctg ggctcaagca atcggcctca gcctccagcg cagctgaagc ttttaggcgc
     3601 cggtggagtg gcggccctcg gcgcgctcgt actgttccac gatggtgtag tcctcgttgt
     3661 gggaggtgat gtccagcttg atgtcggtct tgtaggcgcc gggcagctgc acgggcttct
     3721 tggccatgta ggtggtcttg acctcggcgt cgtagtggcc gccgtccttc agcttcagcc
     3781 tcatcttgat ctcgcccttc agggcgccgt cctcggggta catccgctcg gtggaggcct
     3841 cccagcccat ggtcttcttc tgcattacgg ggccgtcgga ggggaagttg gtgccgcgca
     3901 gcttcacctt gtagatgaac tcgccgtcct gcagggagga gtcctgggtc acggtcacca
     3961 cgccgccgtc ctcgaagttc atcacgcgct cccacttgaa gccctcgggg aaggacagct
     4021 tcaagtagtc ggggatgtcg gcggggtgct tcacgtaggc cttggagccg tactggaact
     4081 gaggggacag gatgtcccag gcgaagggca gggggccgcc cttggtcacc ttcagcttgg
     4141 cggtctgggt gccctcgtag gggcggccct cgccctcgcc ctcgatctcg aactcgtggc
     4201 cgttcacgga gccctccatg cgcaccttga agcgcatgaa ctccttgatg acgtcctcgg
     4261 aggaggccat caactttatt atacaaagtt gcttagcgct ggtgtacttg gtgacggcct
     4321 tggtgccctc ggacacggcg tgcttggcca actccccggg cagcagcagg cgcacggccg
     4381 tctggatctc cctggaggtg atggtcgagc gcttgttgta atgcgccagg cgggaagcct
     4441 cacccgcgat gcgttcgaag atgtcgttga cgaaggagtt catgattccc atggccttag
     4501 aggagatgcc ggtgtcgggg tggacctgct tcagcacctt gtacacgtat acggagtagc
     4561 tctccttgcg gctgcgcttg cgcttcttgc cgtccttctt ctgcgcctta gtcacggctt
     4621 tcttcgagcc cttcttgggc gcgggagcag acttcgctgg ctctggcatg ctagctcacg
     4681 acacctgaaa tggaagaaaa aaactttgaa ccactgtctg aggcttgaga atgaaccaag
     4741 atccaaactc aaaaagggca aattccaagg agaattacat caagtgccaa gctggcctaa
     4801 cttcagtctc cacccactca gtgtggggaa actccatcgc ataaaacccc tccccccaac
     4861 ctaaagacga cgtactccaa aagctcgaga actaatcgag gtgcctggac ggcgcccggt
     4921 actccgtgga gtcacatgaa gcgacggctg aggacggaaa ggcccttttc ctttgtgtgg
     4981 gtgactcacc cgcccgctct cccgagcgcc gcgtcctcca ttttgagctc cctgcagcag
     5041 ggccgggaag cggccatctt tccgctcacg caactggtgc cgaccgggcc agccttgccg
     5101 cccagggcgg ggcgatacac ggcggcgcga ggccaggcac cagagcaggc cggccagctt
     5161 gagactaccc ccgtccgatt ctcggtggcc gcgctcgcag gccccgcctc gccgaacatg
     5221 tgcgctggga cgcacgggcc ccgtcgccgc ccgcggcccc aaaaaccgaa ataccagtgt
     5281 gcagatcttg gcccgcattt acaagactat cttgccagaa aaaaagcgtc gcagcaggtc
     5341 atcaaaaatt ttaaatggct agagacttat cgaaagcagc gagacaggcg cgaaggtgcc
     5401 accagattcg cacgcggcgg ccccagcgcc caggccaggc ctcaactcaa gcacgaggcg
     5461 aaggggctcc ttaagcgcaa ggcctcgaac tctcccaccc acttccaacc cgaagctcgg
     5521 gatcaagaat cacgtactgc agccaggtgg aagtaattca aggcacgcaa gggccataac
     5581 ccgtaaagag gccaggcccg cgggaaccac acacggcact tacctgtgtt ctggcggcaa
     5641 acccgttgcg aaaaagaacg ttcacggcga ctactgcact tatatacggt tctcccccac
     5701 cctcgggaaa aaggcggagc cagtacacga catcactttc ccagtttacc ccgcgccacc
     5761 ttctctaggc accggttcaa ttgccgaccc ctcccccaac ttctcgggga ctgtgggcga
     5821 tgtgcgctct gcccactgac gggcaccgga gcctcacggg atctcccgat ccgtcgacgt
     5881 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5941 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6001 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6061 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6121 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6181 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6241 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6301 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6361 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6421 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6481 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6541 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6601 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6661 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6721 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6781 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6841 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6901 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6961 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7021 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7081 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7141 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     7201 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7261 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7321 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7381 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7441 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7501 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7561 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7621 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7681 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7741 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7801 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7861 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7921 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7981 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     8041 acgccaagct ctagctagag gtcgacggta tacagacatg ataagataca ttgatgagtt
     8101 tggaca
//
