LOCUS       plasmid                 8089 bp    DNA     circular SYN 16-JUL-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(6671..7381)
                     /note="pLannotate"
                     /label="PAmCherry"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(6070..6532)
                     /note="pLannotate"
                     /label="IRES"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      2104..2532
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(1045..1424)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        2585..2689
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          complement(join(7842..8089,1..768))
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             complement(5667..6056)
                     /note="pLannotate"
                     /label="bsd"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2690..3550
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      3721..4309
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(5134..5586)
                     /note="pLannotate"
                     /label="PGK poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="98.1"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(7400..7777)
                     /note="pLannotate"
                     /label="H2B"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="99.2"
                     /fragment="False"
                     /other="CDS"
     promoter        4633..4663
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(769..1043)
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    4597..4618
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(1921..1939)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4732..4750
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    4671..4687
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             1942..2099
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     CDS             1745..1876
                     /note="pLannotate"
                     /label="FP (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="20.6"
                     /fragment="True"
                     /other="CDS"
     CDS             4492..4584
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 cctcccagat ttcggctccg cacagatttg ggacaaagga agtccctgcg ccctctcgca
       61 cgattaccat aaaaggcaat ggctgcggct cgccgcgcct cgacagccgc cggcgctccg
      121 ggggccgccg cgcccctccc ccgagccctc cccggcccga ggcggccccg ccccgcccgg
      181 cacccccacc tgccgccacc ccccgcccgg cacggcgagc cccgcgccac gccccgtacg
      241 gagccccgca cccgaagccg ggccgtgctc agcaactcgg ggaggggggt gcaggggggg
      301 ttgcagcccg accgacgcgc ccacaccccc tgctcacccc cccacgcaca caccccgcac
      361 gcagcctttg ttcccctcgc agcccccccc gcaccgcggg gcaccgcccc cggccgcgct
      421 cccctcgcgc acactgcgga gcgcacaaag ccccgcgccg cgcccgcagc gctcacagcc
      481 gccgggcagc gcggagccgc acgcggcgct ccccacgcac acacacacgc acgcaccccc
      541 cgagccgctc cccccgcaca aagggccctc ccggagcccc tcaaggcttt cacgcagcca
      601 cagaaaagaa acaagccgtc attaaaccaa gcgctaatta cagcccggag gagaagggcc
      661 gtcccgcccg ctcacctgtg ggagtaacgc ggtcagtcag agccggggcg ggcggcgcga
      721 ggcggcggcg gagcggggca cggggcgaag gcagcgcgca gcgactcccg cccgccgcgc
      781 gcttcgcttt ttatagggcc gccgccgccg ccgcctcgcc ataaaaggaa actttcggag
      841 cgcgccgctc tgattggctg ccgccgcacc tctccgcctc gccccgcccc gcccctcgcc
      901 ccgccccgcc ccgcctggcg cgcgcccccc ccccccccgc ccccatcgct gcacaaaata
      961 attaaaaaat aaataaatac aaaattgggg gtggggaggg ggggagatgg ggagagtgaa
     1021 gcagaacgtg gggctcacct cgaccatggt aatagcgatg actaatacgt agatgtactg
     1081 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
     1141 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
     1201 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
     1261 tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
     1321 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
     1381 ccccgtaatt gattactatt aataactagt caataatcaa tgtcgaccgg gatctgatat
     1441 ctataacaag aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat
     1501 ataattatcg tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg
     1561 actcacgcgg tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc
     1621 acgggagctc caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt
     1681 tagaaagaga gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct
     1741 agggttaaaa aagatttgcg ctttactcga cctaaacttt aaacacgtca tagaatcttc
     1801 gtttgacaaa aaccacattg tggccaagct gtgtgacgcg acgcgcgcta aagaatggca
     1861 aaccaagtcg cgcgaggtac ctctcttaag gtagcgcgat cgcacgcgta cccaattcgc
     1921 cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     1981 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     2041 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     2101 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     2161 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     2221 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2281 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     2341 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2401 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     2461 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2521 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     2581 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2641 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2701 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2761 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2821 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     2881 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     2941 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3001 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3061 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3121 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3181 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3241 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3301 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3361 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3421 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3481 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3541 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3601 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3661 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3721 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3781 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3841 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     3901 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     3961 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4021 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4081 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4141 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4201 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4261 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4321 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4381 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     4441 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     4501 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     4561 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     4621 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     4681 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     4741 tcactaaagg gaacaaaagc tggagctagg gataacaggg taatggcgcg ccttaattaa
     4801 ctataacggt cctaaggtag cgagctcggt attcacgaca gcaggctgaa taataaaaaa
     4861 attagaaact attatttaac cctagaaaga taatcatatt gtgacgtacg ttaaagataa
     4921 tcatgcgtaa aattgacgca tgtgttttat cggtctgtat atcgaggttt atttattaat
     4981 ttgaatagat attaagtttt attatattta cacttacata ctaataataa attcaacaaa
     5041 caatttattt atgtttattt atttattaaa aaaaaacaaa aactcaaaat ttcttctata
     5101 aagtaacaaa gggcccgttt aaactcgacc agcttctgat ggaattagaa cttggcaaaa
     5161 caatactgag aatgaagtgt atgtggaaca gaggctgctg atctcgttct tcaggctatg
     5221 aaactgacac atttggaaac cacagtactt agaaccacaa agtgggaatc aagagaaaaa
     5281 caatgatccc acgagagatc tatagatcta tagatcatga gtgggaggaa tgagctggcc
     5341 cttaatttgg ttttgcttgt ttaaattatg atatccaact atgaaacatt atcataaagc
     5401 aatagtaaag agccttcagt aaagagcagg catttatcta atcccacccc acccccaccc
     5461 ccgtagctcc aatccttcca ttcaaaatgt aggtactctg ttctcaccct tcttaacaaa
     5521 gtatgacagg aaaaacttcc attttagtgg acatctttat tgtttaatag atcatcaatt
     5581 tctgcatccc gtcaccatca actcatgctc ggttcatcta gtaacagaaa gtagcttgaa
     5641 tgtacaagat caatggctgg tgcttagccc tcccacacat aaccagaggg cagcaattca
     5701 cgaatcccaa ctgccgtcgg ctgtccatca ctgtccttca ctatggcttt gatcccagga
     5761 tgcagatcga gaagcacctg tcggcaccgt ccgcaggggc tcaagatgcc cctgttctca
     5821 tttccgatcg cgacgataca agtcaggttg ccagctgccg cagcagcagc agtgcccagc
     5881 accacgagtt ctgcacaagg tcccccagta aaatgatata cattgacacc agtgaagatg
     5941 cggccgtcgc tagagagagc tgcgctggcg acgctgtagt cttcagagat ggggatgctg
     6001 ttgattgtag ccgttgctct ttcaatgagg gtggattctt cttgagacaa aggcatggtt
     6061 gtggccatat tatcatcgtg tttttcaaag gaaaaccacg tccccgtggt tcggggggcc
     6121 tagacgtttt tttaacctcg actaaacaca tgtaaagcat gtgcaccgag gccccagatc
     6181 agatcccata caatggggta ccttctgggc atccttcagc cccttgttga atacgcttga
     6241 ggagagccat ttgactcttt ccacaactat ccaactcaca acgtggcact ggggttgtgc
     6301 cgcctttgca ggtgtatctt atacacgtgg cttttggccg cagaggcacc tgtcgccagg
     6361 tggggggttc cgctgcctgc aaagggtcgc tacagacgtt gtttgtcttc aagaagcttc
     6421 cagaggaact gcttccttca cgacattcaa cagaccttgc attcctttgg cgagagggga
     6481 aagaccccta ggaatgctcg tcaagaagac agggccaggt ttccgggccc tcacattgcc
     6541 aaaagacggc aatatggtgg aaaataacat atagacaaac gcacaccggc cttattccaa
     6601 gcggcttcgg ccagtaacgt tagggggggg ggagggagag gggggggggg cggaattcgt
     6661 taacctcgag ttacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggcac
     6721 gttcgtactg ttccacgatg gtgtagtcct cgttgtgtga ggtgatgtcc aacttgcggt
     6781 tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg gtcttgacct
     6841 cagcgtcgta gtggccgccg tccttcagct tcactctcgg cttgacctcg cccttcaggg
     6901 cgccgtcctc ggggtacatc cgctcggaga gggcctccca gcccatggtc ttcttctgca
     6961 ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag atgaactcac
     7021 cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg aatttcatca
     7081 cgcgctccca cttgaagccc tcggggaagg acagcttaaa gtagtcgggg atgtcggcgg
     7141 ggtgcttcac gtaggcattg gagccgtaca tgaattgagg ggacaggatg tcccaggtga
     7201 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
     7261 ggccctcgcc ctcgccctcg atctcgaaca cgtggccgtt cacggacccc tccatgtgca
     7321 ccttgaagcg catgaactcc ttaatgatgg ccatgttatc ctcctcgccc ttgctcacca
     7381 tggtggcgac cggtggatcc ttagcgctgg tgtacttggt gatggcctta gtaccctcgg
     7441 acacggcgtg cttggccaac tccccaggca gcagcaggcg cacggccgtc tggatctccc
     7501 tggaggtgat ggtcgagcgc ttgttgtaat gcgccaggcg ggaagcctca cctgcgatgc
     7561 gctcgaaaat gtcgttcaca aacgaattca tgatgcccat ggccttggac gaaatgccgg
     7621 tgtcagggtg gacctgcttc agaaccttgt acacatagat ggaatagctc tccttgcggc
     7681 tgcgcttgcg cttcttgccg cctttcttct gcgccttagt caccgccttc ttggagccct
     7741 ttttcggggc gggagcagac ttcgctggct ctggcatctc gagagatcaa ttctttgcca
     7801 aaatgatgag acagcacaat aaccagcacg ttgcccagga gctgtaggaa aaagaagaag
     7861 gcatgaacat ggttagcaga ggctctagag ccgccggtca cacgccagaa gccgaacccc
     7921 gccctgcccc gtccccccga aggcagccgt ccccccgcgg acagccccga ggctggagag
     7981 ggagaagggg acggcggcgc ggcgacgcac gaaggccctc cccgcccatt tccttcctgc
     8041 cggcgccgca ccgcttcgcc ccgcgcccgc tagagggggt gcggcggcg
//
