LOCUS       plasmid                 8230 bp    DNA     circular SYN 16-JUL-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     promoter        5138..6316
                     /note="pLannotate"
                     /label="EF-1α promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             6831..7541
                     /note="pLannotate"
                     /label="PAmCherry"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(4019..4447)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    7693..7917
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(3862..3966)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(3001..3861)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             452..1255
                     /note="pLannotate"
                     /label="NeoR/KanR"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(2242..2830)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        join(8170..8230,1..438)
                     /note="pLannotate"
                     /label="PGK promoter"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    1262..1310
                     /note="pLannotate"
                     /label="HSV TK poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             7608..7649
                     /note="pLannotate"
                     /label="V5 tag"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             6435..6812
                     /note="pLannotate"
                     /label="H2B"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="99.2"
                     /fragment="False"
                     /other="CDS"
     protein_bind    complement(8075..8108)
                     /note="pLannotate"
                     /label="loxP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    complement(1329..1362)
                     /note="pLannotate"
                     /label="loxP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(1888..1918)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(1933..1954)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        4612..4630
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        6333..6351
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(1801..1819)
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(1864..1880)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(4452..4609)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(7917..7970)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="50.0"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(4675..4806)
                     /note="pLannotate"
                     /label="FP (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="20.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1967..2059)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
       61 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
      121 gaagttcccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
      181 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
      241 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
      301 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
      361 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
      421 tcatctccgg gcctttcgac ctgcagccaa tatgggatcg gccattgaac aagatggatt
      481 gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca
      541 gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct
      601 ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct
      661 atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc
      721 gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct
      781 tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga
      841 tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg
      901 gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc
      961 agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac
     1021 ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat
     1081 cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga
     1141 tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc
     1201 cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagggga
     1261 tcggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc ggatccggaa
     1321 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgaagaggt
     1381 tcactagtac tggccattgc ggccttaatt aaggtaccgg cgcgccgttt aaacgggccc
     1441 tttgttactt tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat
     1501 aaataaattg tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat
     1561 atctattcaa attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt
     1621 ttacgcatga ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaaataat
     1681 agtttctaat ttttttatta ttcagcctgc tgtcgtgaat accgagctcg ctaccttagg
     1741 accgttatag ttaattaagg cgcgccatta ccctgttatc cctagctcca gcttttgttc
     1801 cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1861 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     1921 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1981 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     2041 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     2101 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     2161 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     2221 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     2281 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     2341 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     2401 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     2461 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     2521 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     2581 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     2641 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2701 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2761 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2821 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2881 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2941 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     3001 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3061 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3121 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     3181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3961 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     4021 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     4081 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     4141 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     4201 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     4261 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     4321 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     4381 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     4441 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     4501 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     4561 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac
     4621 tcactatagg gcgaattggg tacgcgtgcg atcgcgctac cttaagagag gtacctcgcg
     4681 cgacttggtt tgccattctt tagcgcgcgt cgcgtcacac agcttggcca caatgtggtt
     4741 tttgtcaaac gaagattcta tgacgtgttt aaagtttagg tcgagtaaag cgcaaatctt
     4801 ttttaaccct agaaagatag tctgcgtaaa attgacgcat gcattcttga aatattgctc
     4861 tctctttcta aatagcgcga atccgtcgct gtgcatttag gacatctcag tcgccgcttg
     4921 gagctcccgt gaggcgtgct tgtcaatgcg gtaagtgtca ctgattttga actataacga
     4981 ccgcgtgagt caaaatgacg catgattatc ttttacgtga cttttaagat ttaactcata
     5041 cgataattat attgttattt catgttctac ttacgtgata acttattata tatatatttt
     5101 cttgttatag atatcggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
     5161 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     5221 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     5281 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     5341 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     5401 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     5461 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     5521 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     5581 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     5641 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     5701 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     5761 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     5821 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     5881 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     5941 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     6001 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     6061 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     6121 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     6181 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     6241 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     6301 atttcaggtg tcgtgaggaa ttagcttggt actaatacga ctcactatag ggagacccaa
     6361 gctggctagg taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg
     6421 cagatctagc tagcatgcca gagccagcga agtctgctcc cgccccgaaa aagggctcca
     6481 agaaggcggt gactaaggcg cagaagaaag gcggcaagaa gcgcaagcgc agccgcaagg
     6541 agagctattc catctatgtg tacaaggttc tgaagcaggt ccaccctgac accggcattt
     6601 cgtccaaggc catgggcatc atgaattcgt ttgtgaacga cattttcgag cgcatcgcag
     6661 gtgaggcttc ccgcctggcg cattacaaca agcgctcgac catcacctcc agggagatcc
     6721 agacggccgt gcgcctgctg ctgcctgggg agttggccaa gcacgccgtg tccgagggta
     6781 ctaaggccat caccaagtac accagcgcta aggatccacc ggtcgccacc atggtgagca
     6841 agggcgagga ggataacatg gccatcatta aggagttcat gcgcttcaag gtgcacatgg
     6901 aggggtccgt gaacggccac gtgttcgaga tcgagggcga gggcgagggc cgcccctacg
     6961 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcacctggg
     7021 acatcctgtc ccctcaattc atgtacggct ccaatgccta cgtgaagcac cccgccgaca
     7081 tccccgacta ctttaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaaat
     7141 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggtgagttca
     7201 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga
     7261 agaccatggg ctgggaggcc ctctccgagc ggatgtaccc cgaggacggc gccctgaagg
     7321 gcgaggtcaa gccgagagtg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     7381 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aaccgcaagt
     7441 tggacatcac ctcacacaac gaggactaca ccatcgtgga acagtacgaa cgtgccgagg
     7501 gccgccactc caccggcggc atggacgagc tgtacaagta agcggccgct tttttcctta
     7561 tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
     7621 ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagtt taaacccgct
     7681 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     7741 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     7801 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     7861 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     7921 gatatcgaat tcctgcagcc cgggggatcc actagttcta gagcggccgc acgtctaaga
     7981 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     8041 tcaagaattc cgatcatatt caataaccct taatataact tcgtataatg tatgctatac
     8101 gaagttatta ggtctgaaga ggagtttacg tccagccaag cttaggggcc cctgcaggtc
     8161 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     8221 ccccgctggg
//
