LOCUS       plasmid                 7564 bp    DNA     circular SYN 13-JUL-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     promoter        join(7070..7564,1..684)
                     /note="pLannotate"
                     /label="EF-1α promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             806..1522
                     /note="pLannotate"
                     /label="EGFP"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    2677..2901
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    4392..4513
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(6584..6688)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             3240..4262
                     /note="pLannotate"
                     /label="HygR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.7"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(5723..6583)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(4964..5552)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    3185..3232
                     /note="pLannotate"
                     /label="FRT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             2592..2633
                     /note="pLannotate"
                     /label="V5 tag"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(4610..4640)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             1801..2535
                     /note="pLannotate"
                     /label="FBL (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="76.3"
                     /fragment="True"
                     /other="CDS"
     protein_bind    complement(4655..4676)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        701..719
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(4586..4602)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      2947..3180
                     /note="pLannotate"
                     /label="f1 ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="65.2"
                     /fragment="True"
                     /other="rep_origin"
     misc_feature    1523..1573
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="77.3"
                     /fragment="True"
                     /other="misc_feature"
     CDS             4272..4397
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="97.6"
                     /match_length="33.3"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(6747..6799)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             6997..7063
                     /note="pLannotate"
                     /label="small t antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4689..4781)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     promoter        2905..2926
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
     enhancer        6954..6972
                     /note="pLannotate"
                     /label="CMV enhancer (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.0"
                     /fragment="True"
                     /other="enhancer"
ORIGIN
        1 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
       61 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      121 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      181 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      241 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      301 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
      361 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
      421 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
      481 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
      541 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
      601 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
      661 tttcttccat ttcaggtgtc gtgaggaatt agcttggtac taatacgact cactataggg
      721 agacccaagc tggctaggta agcttggtac cgagctcgga tccactagtc cagtgtggtg
      781 gaattctgca gatccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
      841 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      901 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      961 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
     1021 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1081 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1141 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1201 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1261 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     1321 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1381 ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc
     1441 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1501 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt cgaattctgc
     1561 agtcgacggt accatgaagc caggattcag tccccgtggg ggtggctttg gcggccgagg
     1621 gggctttggt gaccgtggtg gtcgtggagg ccgagggggc tttggcgggg gccgaggtcg
     1681 aggcggaggc tttagaggtc gtggacgagg aggaggtgga ggcggcggcg gcggtggagg
     1741 aggaggaaga ggtggtggag gcttccattc tggtggcaac cggggtcgtg gtcggggagg
     1801 aaaagaggaa accagtcggg gaagaatgtg atggtggagc cgcatcggca tgagggtgtc
     1861 ttcatttgtc gaggaaagga agatgcactg gtcaccaaga acctggtccc tggggaatca
     1921 gtttatggag agaagagagt ctcgatttcg gaaggagatg acaaaattga gtaccgagcc
     1981 tggaacccct tccgctccaa gctagcagca gcaatcctgg gtggtgtgga ccagatccac
     2041 atcaaaccgg gggctaaggt tctctacctc ggggctgcct cgggcaccac ggtctcccat
     2101 gtctctgaca tcgttggtcc ggatggtcta gtctatgcag tcgagttctc ccaccgctct
     2161 ggccgtgacc tcattaactt ggccaagaag aggaccaaca tcattcctgt gatcgaggat
     2221 gctcgacacc cacacaaata ccgcatgctc atcgcaatgg tggatgtgat ctttgctgat
     2281 gtggcccagc cagaccagac ccggattgtg gccctgaatg cccacacctt cctgcgtaat
     2341 ggaggacact ttgtgatttc cattaaggcc aactgcattg actccacagc ctcagccgag
     2401 gccgtgtttg cctccgaagt gaaaaagatg caacaggaga acatgaagcc gcaggagcag
     2461 ttgacccttg agccatatga aagagaccat gccgtggtcg tgggagtgta caggccaccc
     2521 cccaaggtga agaactgagg atcatccagc acagtggcgg ccgctcgagt ctagagggcc
     2581 cgcggttcga aggtaagcct atccctaacc ctctcctcgg tctcgattct acgcgtaccg
     2641 gttagtaatg agtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
     2701 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
     2761 tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg
     2821 ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg
     2881 gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt
     2941 atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     3001 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     3061 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     3121 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     3181 cctagaagtt cctattccga agttcctatt ctctagaaag tataggaact tccttggcca
     3241 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
     3301 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
     3361 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
     3421 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
     3481 aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag
     3541 acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc atggatgcga
     3601 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
     3661 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
     3721 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
     3781 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
     3841 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
     3901 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
     3961 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc
     4021 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
     4081 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
     4141 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
     4201 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
     4261 agcacgtact acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     4321 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     4381 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4441 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4501 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     4561 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     4621 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     4681 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     4741 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     4801 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     4861 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     4921 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     4981 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     5041 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     5101 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     5161 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     5221 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     5281 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     5341 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     5401 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     5461 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     5521 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     5581 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     5641 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     5701 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     5761 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5821 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5881 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5941 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     6001 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     6061 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     6121 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     6181 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     6241 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     6301 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     6361 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     6421 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     6481 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6541 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6601 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     6661 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     6721 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     6781 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     6841 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     6901 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     6961 attattgact aggcttttgc aaaaagcttt gcaaagatgg ataaagtttt aaacagagag
     7021 gaatctttgc agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc
     7081 ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg
     7141 caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta
     7201 ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt
     7261 gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc
     7321 ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg
     7381 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
     7441 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
     7501 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
     7561 tcta
//
