LOCUS       plasmid                 8307 bp    DNA     circular SYN 13-JUL-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     misc_feature    complement(5481..5943)
                     /note="pLannotate"
                     /label="IRES"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      1515..1943
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(456..835)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        1996..2100
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     intron          complement(join(7471..8307,1..179))
                     /note="pLannotate"
                     /label="chimeric intron"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             complement(5078..5467)
                     /note="pLannotate"
                     /label="bsd"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(6079..6962)
                     /note="pLannotate"
                     /label="HaloTag® (fragment)"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="99.2"
                     /fragment="True"
                     /other="CDS"
     CDS             2101..2961
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      3132..3720
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(4545..4997)
                     /note="pLannotate"
                     /label="PGK poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="98.1"
                     /fragment="False"
                     /other="polyA_signal"
     CDS             complement(7029..7406)
                     /note="pLannotate"
                     /label="H2B"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="99.2"
                     /fragment="False"
                     /other="CDS"
     promoter        4044..4074
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(180..454)
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    4008..4029
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(1332..1350)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        4143..4161
                     /note="pLannotate"
                     /label="T3 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    4082..4098
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             1353..1510
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     CDS             1156..1287
                     /note="pLannotate"
                     /label="FP (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="20.6"
                     /fragment="True"
                     /other="CDS"
     CDS             3903..3995
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 tcacgcagcc acagaaaaga aacaagccgt cattaaacca agcgctaatt acagcccgga
       61 ggagaagggc cgtcccgccc gctcacctgt gggagtaacg cggtcagtca gagccggggc
      121 gggcggcgcg aggcggcggc ggagcggggc acggggcgaa ggcagcgcgc agcgactccc
      181 gcccgccgcg cgcttcgctt tttatagggc cgccgccgcc gccgcctcgc cataaaagga
      241 aactttcgga gcgcgccgct ctgattggct gccgccgcac ctctccgcct cgccccgccc
      301 cgcccctcgc cccgccccgc cccgcctggc gcgcgccccc cccccccccg cccccatcgc
      361 tgcacaaaat aattaaaaaa taaataaata caaaattggg ggtggggagg gggggagatg
      421 gggagagtga agcagaacgt ggggctcacc tcgaccatgg taatagcgat gactaatacg
      481 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg
      541 ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt
      601 actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct
      661 attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc
      721 ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta
      781 tgaactaatg accccgtaat tgattactat taataactag tcaataatca atgtcgaccg
      841 ggatctgata tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat
      901 gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat
      961 gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac
     1021 aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga
     1081 ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag
     1141 actatctttc tagggttaaa aaagatttgc gctttactcg acctaaactt taaacacgtc
     1201 atagaatctt cgtttgacaa aaaccacatt gtggccaagc tgtgtgacgc gacgcgcgct
     1261 aaagaatggc aaaccaagtc gcgcgaggta cctctcttaa ggtagcgcga tcgcacgcgt
     1321 acccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
     1381 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     1441 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     1501 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     1561 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     1621 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     1681 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     1741 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     1801 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     1861 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     1921 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     1981 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     2041 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     2101 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     2161 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     2221 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     2281 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     2341 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     2401 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     2461 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     2521 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     2581 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     2641 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     2701 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     2761 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     2821 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     2881 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     2941 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     3001 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     3061 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3241 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     3301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     3721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     3781 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     3841 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     3901 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3961 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     4021 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     4081 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
     4141 gaaattaacc ctcactaaag ggaacaaaag ctggagctag ggataacagg gtaatggcgc
     4201 gccttaatta actataacgg tcctaaggta gcgagctcgg tattcacgac agcaggctga
     4261 ataataaaaa aattagaaac tattatttaa ccctagaaag ataatcatat tgtgacgtac
     4321 gttaaagata atcatgcgta aaattgacgc atgtgtttta tcggtctgta tatcgaggtt
     4381 tatttattaa tttgaataga tattaagttt tattatattt acacttacat actaataata
     4441 aattcaacaa acaatttatt tatgtttatt tatttattaa aaaaaaacaa aaactcaaaa
     4501 tttcttctat aaagtaacaa agggcccgtt taaactcgac cagcttctga tggaattaga
     4561 acttggcaaa acaatactga gaatgaagtg tatgtggaac agaggctgct gatctcgttc
     4621 ttcaggctat gaaactgaca catttggaaa ccacagtact tagaaccaca aagtgggaat
     4681 caagagaaaa acaatgatcc cacgagagat ctatagatct atagatcatg agtgggagga
     4741 atgagctggc ccttaatttg gttttgcttg tttaaattat gatatccaac tatgaaacat
     4801 tatcataaag caatagtaaa gagccttcag taaagagcag gcatttatct aatcccaccc
     4861 cacccccacc cccgtagctc caatccttcc attcaaaatg taggtactct gttctcaccc
     4921 ttcttaacaa agtatgacag gaaaaacttc cattttagtg gacatcttta ttgtttaata
     4981 gatcatcaat ttctgcatcc cgtcaccatc aactcatgct cggttcatct agtaacagaa
     5041 agtagcttga atgtacaaga tcaatggctg gtgcttagcc ctcccacaca taaccagagg
     5101 gcagcaattc acgaatccca actgccgtcg gctgtccatc actgtccttc actatggctt
     5161 tgatcccagg atgcagatcg agaagcacct gtcggcaccg tccgcagggg ctcaagatgc
     5221 ccctgttctc atttccgatc gcgacgatac aagtcaggtt gccagctgcc gcagcagcag
     5281 cagtgcccag caccacgagt tctgcacaag gtcccccagt aaaatgatat acattgacac
     5341 cagtgaagat gcggccgtcg ctagagagag ctgcgctggc gacgctgtag tcttcagaga
     5401 tggggatgct gttgattgta gccgttgctc tttcaatgag ggtggattct tcttgagaca
     5461 aaggcatggt tgtggccata ttatcatcgt gtttttcaaa ggaaaaccac gtccccgtgg
     5521 ttcggggggc ctagacgttt ttttaacctc gactaaacac atgtaaagca tgtgcaccga
     5581 ggccccagat cagatcccat acaatggggt accttctggg catccttcag ccccttgttg
     5641 aatacgcttg aggagagcca tttgactctt tccacaacta tccaactcac aacgtggcac
     5701 tggggttgtg ccgcctttgc aggtgtatct tatacacgtg gcttttggcc gcagaggcac
     5761 ctgtcgccag gtggggggtt ccgctgcctg caaagggtcg ctacagacgt tgtttgtctt
     5821 caagaagctt ccagaggaac tgcttccttc acgacattca acagaccttg cattcctttg
     5881 gcgagagggg aaagacccct aggaatgctc gtcaagaaga cagggccagg tttccgggcc
     5941 ctcacattgc caaaagacgg caatatggtg gaaaataaca tatagacaaa cgcacaccgg
     6001 ccttattcca agcggcttcg gccagtaacg ttaggggggg gggagggaga gggggggggg
     6061 cggaattcgt taacttaacc ggaaatctcc agagtagaca gccagcgcgc gatctcgctg
     6121 ccgatcaggt ccgggttgtc ttcttgcagc agattcagac ccgggccgat gtccacagcc
     6181 ttgcagttag gcaggctttt ggccaggcga gcggcttcgg ccggtgggat cagaacgcct
     6241 ggggtgcccc agaacagcag cttcgggaca ggggactggt gcagccagtc catgtattct
     6301 tcgaccagcg cgacgatgtt cgctggctca ccggcgattg gcagctcgtt tgggaagcgc
     6361 cacagtggct cgcggtcaac aggattcagg aacggctcgc ggtaatggtc catctcgact
     6421 tcagtcagcg ggcggacgac acccatcggc agcgtaccct cgataaaaac gttctgatcg
     6481 atgatcagct tgcggccgac gtcggtggtg cggaaggcct ggaaggtctc gcgggcaaat
     6541 tctggccatt cgtcccaggt cgggataggg cggatgaact ccataaatgc aatacctttg
     6601 acgcgctctg gattgcgctt ggcccagtgg aaacccagag cggagcccca gtcgtgaatg
     6661 accaggacga cctcttccag acccagggct tcgatgaagg catccatgaa gcggacgtgg
     6721 tcgtcgaaga aataacccag gtctggtttg tcggatttgc ccataccgat caggtctgga
     6781 gcaatgcagc gatgggtcgg tgcaacatgc gggatgatgt tgcgccacac gtaggaggag
     6841 gtcgggttac cgtgcaggaa cagcacaggg gtgccatcgc gcggaccaac atcgacgtag
     6901 tgcatgcgct cgcccaggac ttccacataa tgggggtcga atggaaagcc agtaccgatt
     6961 tcggaaccgc caccagagcc tccacctcca cttccacctc cgcctgaacc acctccacca
     7021 gatggatcct tagcgctggt gtacttggtg atggccttag taccctcgga cacggcgtgc
     7081 ttggccaact ccccaggcag cagcaggcgc acggccgtct ggatctccct ggaggtgatg
     7141 gtcgagcgct tgttgtaatg cgccaggcgg gaagcctcac ctgcgatgcg ctcgaaaatg
     7201 tcgttcacaa acgaattcat gatgcccatg gccttggacg aaatgccggt gtcagggtgg
     7261 acctgcttca gaaccttgta cacatagatg gaatagctct ccttgcggct gcgcttgcgc
     7321 ttcttgccgc ctttcttctg cgccttagtc accgccttct tggagccctt tttcggggcg
     7381 ggagcagact tcgctggctc tggcatctcg agagatcaat tctttgccaa aatgatgaga
     7441 cagcacaata accagcacgt tgcccaggag ctgtaggaaa aagaagaagg catgaacatg
     7501 gttagcagag gctctagagc cgccggtcac acgccagaag ccgaaccccg ccctgccccg
     7561 tccccccgaa ggcagccgtc cccccgcgga cagccccgag gctggagagg gagaagggga
     7621 cggcggcgcg gcgacgcacg aaggccctcc ccgcccattt ccttcctgcc ggcgccgcac
     7681 cgcttcgccc cgcgcccgct agagggggtg cggcggcgcc tcccagattt cggctccgca
     7741 cagatttggg acaaaggaag tccctgcgcc ctctcgcacg attaccataa aaggcaatgg
     7801 ctgcggctcg ccgcgcctcg acagccgccg gcgctccggg ggccgccgcg cccctccccc
     7861 gagccctccc cggcccgagg cggccccgcc ccgcccggca cccccacctg ccgccacccc
     7921 ccgcccggca cggcgagccc cgcgccacgc cccgtacgga gccccgcacc cgaagccggg
     7981 ccgtgctcag caactcgggg aggggggtgc agggggggtt gcagcccgac cgacgcgccc
     8041 acaccccctg ctcacccccc cacgcacaca ccccgcacgc agcctttgtt cccctcgcag
     8101 ccccccccgc accgcggggc accgcccccg gccgcgctcc cctcgcgcac actgcggagc
     8161 gcacaaagcc ccgcgccgcg cccgcagcgc tcacagccgc cgggcagcgc ggagccgcac
     8221 gcggcgctcc ccacgcacac acacacgcac gcaccccccg agccgctccc cccgcacaaa
     8281 gggccctccc ggagcccctc aaggctt
//
