LOCUS       6K8FHY_1_pLEX305       12900 bp    DNA     circular SYN 26-SEP-2025
DEFINITION  pLEX305_HA-CIC-LEUTX_maxi_092525.
ACCESSION   pLEX305_HA-CIC-LEUTX_maxi_092525
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..12900
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=high-copy-number ColE1/pMB1/pBR322/pUC 
                     origin
                     of replication "
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     repeat_region   complement(749..760)
                     /label=AAV2 ITR (fragment)
                     /note="pLannotate"
                     /note="/database=itrs"
                     /note="/description=AAV2 ITR Flip"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.3"
                     /note="/other=repeat_region"
     CDS             772..864
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=lac repressor; lacI; The lac repressor 
                     binds
                     to the lac operator to inhibit transcription in E. coli. 
                     This inhibition can be relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
     protein_bind    877..898
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=CAP binding activates transcription in 
                     the
                     presence of cAMP. E. coli catabolite activator protein"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        913..943
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=promoter for the E. coli lac operon"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    947..969
                     /label=lac operator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=The lac repressor binds to the lac 
                     operator
                     to inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). lac 
                     repressor encoded by lacI"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=92.0"
                     /note="/other=protein_bind"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=promoter for bacteriophage T3 RNA 
                     polymerase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=Rous sarcoma virus enhancer/promoter"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=truncated 5' long terminal repeat (LTR)
                     from
                     HIV-1"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=packaging signal of human 
                     immunodeficiency
                     virus type 1"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             1621..1984
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=gag protein from human immunodeficiency
                     virus
                     1; gag"
                     /note="/fragment=True"
                     /note="/identity=97.3"
                     /note="/match_length=24.2"
                     /note="/other=CDS"
     misc_feature    2130..2363
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=The Rev response element (RRE) of HIV-1
                     allows for Rev-dependent mRNA export from the nucleus to 
                     the cytoplasm."
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             2740..2766
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=human immunodeficiency virus 1 (HIV-1) 
                     Rev
                     protein; rev; enhances nuclear export of unspliced and 
                     incompletely spliced viral mRNAs"
                     /note="/fragment=True"
                     /note="/identity=96.3"
                     /note="/match_length=7.7"
                     /note="/other=CDS"
     promoter        2773..2796
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=SV40 enhancer and early promoter"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=12.2"
                     /note="/other=promoter"
     promoter        2797..2868
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=SV40 enhancer and early promoter"
                     /note="/fragment=True"
                     /note="/identity=98.6"
                     /note="/match_length=24.2"
                     /note="/other=promoter"
     rep_origin      2892..3027
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=SV40 origin of replication"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             3073..3669
                     /label=PUAC_STRAD
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/description=Protein evidence at protein level: 
                     Swiss-Prot
                     protein existence level 1. Detoxification of puromycin. 
                     From Streptomyces alboniger."
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     misc_feature    3718..3835
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=central polypurine tract and central
                     termination sequence of HIV-1"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        3893..4393
                     /label=hPGK promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=human phosphoglycerate kinase 1 
                     promoter"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    4414..4438
                     /label=attB1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=mutant version of attB; recombination 
                     site
                     for the Gateway(R) BP reaction BP Clonase(TM)"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             4441..9732
                     /codon_start=1
                     /label=HA-CIC-LEUTX
                     /translation="MAGYPYDVPDYASLYPYDVPDYASLYPYDVPDYASLRSSRGVHHM
                     LSLRIRYRAPRYRSRAAAMYSAHRPLMPASSAASRGLGMFVWTNVEPRSVAVFPWHSLV
                     PFLAPSQPDPSVQPSEAQQPASHPVASNQSKEPAESAAVAHERPPGGTGSADPERPPGA
                     TCPESPGPGPPHPLGVVESGKGPPPTTEEEASGPPGEPRLDSETESDHDDAFLSIMSPE
                     IQLPLPPGKRRTQSLSALPKERDSSSEKDGRSPNKREKDHIRRPMNAFMIFSKRHRALV
                     HQRHPNQDNRTVSKILGEWWYALGPKEKQKYHDLAFQVKEAHFKAHPDWKWCNKDRKKS
                     SSEAKPTSLGLAGGHKETRERSMSETGTAAAPGVSSELLSVAAQTLLSSDTKAPGSSSC
                     GAERLHTVGGPGSARPRAFSHSGVHSLDGGEVDSQALQELTQMVSGPASYSGPKPSTQY
                     GAPGPFAAPGEGGALAATGRPPLLPTRASRSQRAASEDMTSDEERMVICEEEGDDDVIA
                     DDGFGTTDIDLKCKERVTDSESGDSSGEDPEGNKGFGRKVFSPVIRSSFTHCRPPLDPE
                     PPGPPDPPVAFGKGYGSAPSSSASSPASSSASAATSFSLGSGTFKAQESGQGSTAGPLR
                     PPPPGAGGPATPSKATRFLPMDPATFRRKRPESVGGLEPPGPSVIAAPPSGGGNILQTL
                     VLPPNKEEQEGGGARVPSAPAPSLAYGAPAAPLSRPAATMVTNVVRPVSSTPVPIASKP
                     FPTSGRAEASPNDTAGARTEMGTGSRVPGGSPLGVSLVYSDKKSAAATSPAPHLVAGPL
                     LGTVGKAPATVTNLLVGTPGYGAPAPPAVQFIAQGAPGGGTTAGSGAGAGSGPNGPVPL
                     GILQPGALGKAGGITQVQYILPTLPQQLQVAPAPAPAPGTKAAAPSGPAPTTSIRFTLP
                     PGTSTNGKVLAATAPTPGIPILQSVPSAPPPKAQSVSPVQAPPPGGSAQLLPGKVLVPL
                     AAPSMSVRGGGAGQPLPLVSPPFSVPVQNGAQPPSKIIQLTPVPVSTPSGLVPPLSPAT
                     LPGPTSQPQKVLLPSSTRITYVQSAGGHALPLGTSPASSQAGTVTSYGPTSSVALGFTS
                     LGPSGPAFVQPLLSAGQAPLLAPGQVGVSPVPSPQLPPACAAPGGPVITAFYSGSPAPT
                     SSAPLAQPSQAPPSLVYTVATSTTPPAATILPKGPPAPATATPAPTSPFPSATAGSMTY
                     SLVAPKAQRPSPKAPQKVKAAIASIPVGSFEAGASGRPGPAPRQPLEPGPVREPTAPES
                     ELEGQPTPPAPPPLPETWTPTARSSPPLPPPAEERTSAKGPETMASKFPSSSSDWRVPG
                     QGLENRGEPPTPPSPAPAPAVAPGGSSESSSGRAAGDTPERKEAAGTGKKVKVRPPPLK
                     KTFDSVDNRVLSEVDFEERFAELPEFRPEEVLPSPTLQSLATSPRAILGSYRKKRKNST
                     DLDSAPEDPTSPKRKMRRRSSCSSEPNTPKSAKCEGDIFTFDRTGTEAEDVLGELEYDK
                     VPYSSLRRTLDQRRALVMQLFQDHGFFPSAQATAAFQARYADIFPSKVCLQLKIREVRQ
                     KIMQAATPTEQPPGAEAPLPVPPPTGTAAAPAPTPSPAGGPDPTSPSSDSGTAQAAPPL
                     PPPPESGPGQPGWEGAPQPSPPPPGPSTAATGGISDANDHDLREPSGIKNPGGASASAR
                     VSSWDSQSYDIEQICLGASNPPWASTLFEIDEFVKIYDLPGEDDTSSLNQYLFPVCLEY
                     DQLQSSV"
     misc_feature    4441..4629
                     /label=3x HA tag and linker
     misc_feature    4630..9450
                     /label=CIC
     misc_feature    9451..9732
                     /label=LEUTX
     protein_bind    complement(9734..9758)
                     /label=attB2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=mutant version of attB; recombination 
                     site
                     for the Gateway(R) BP reaction BP Clonase(TM)"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    9810..10398
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=woodchuck hepatitis virus 
                     posttranscriptional
                     regulatory element"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    10449..10468
                     /label=cPPT/CTS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=central polypurine tract and central
                     termination sequence of HIV-1"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.9"
                     /note="/other=misc_feature"
     LTR             10470..10703
                     /label=3' LTR (Delta-U3)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=self-inactivating 3' long terminal 
                     repeat
                     (LTR) from HIV-1"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     polyA_signal    10781..10902
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=SV40 polyadenylation signal"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      10942..11077
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=SV40 origin of replication"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        complement(11098..11116)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=promoter for bacteriophage T7 RNA 
                     polymerase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             11124..11279
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=LacZ-alpha fragment of 
                     beta-galactosidase; lacZ
                     fragment"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
     rep_origin      11284..11712
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=f1 bacteriophage origin of replication;
                     arrow
                     indicates direction of (+) strand synthesis "
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        11765..11869
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=bla"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             11870..12730
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/description=beta-lactamase; bla; confers resistance
                     to
                     ampicillin, carbenicillin, and related antibiotics"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     2821 cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata gtcccgcccc
     2881 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     2941 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3001 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct agaggtacca
     3061 ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc
     3121 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc
     3181 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc
     3241 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt ggcggtctgg
     3301 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc
     3361 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac
     3421 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc
     3481 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg
     3541 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc
     3601 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag
     3661 cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca tccacaattt
     3721 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     3781 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     3841 ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca ggttgcgcct
     3901 tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa
     3961 acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg
     4021 gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg
     4081 ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac
     4141 tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc
     4201 tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt
     4261 gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc
     4321 attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga
     4381 cctctctccc caggggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctta
     4441 atggcaggtt acccatacga cgttcctgac tatgcgtcac tctaccccta tgacgtaccg
     4501 gattatgcat ccctatatcc gtatgatgtt ccagattacg cttctctacg ttcctctaga
     4561 ggcgtccacc atatgttatc acttcgaatt cgatatcgag ctcccaggta ccgatctcga
     4621 gcggccgcca tgtattcggc ccacaggccc ctgatgcccg cgtccagcgc ggcctcccgt
     4681 ggcctcggca tgttcgtgtg gacgaatgtg gaacctcgct ctgtggctgt gttcccttgg
     4741 cactccttag tccccttcct ggcacccagc cagcctgacc cctccgtgca gccgagcgag
     4801 gcccagcaac ctgccagcca cccagtggcc tccaaccaga gcaaagaacc tgctgagtcg
     4861 gcagctgttg ctcatgaacg gccaccaggt gggacaggga gtgctgaccc tgagcggccc
     4921 cctggagcca catgccctga gagcccagga cccggacccc cacacccttt gggggtggtg
     4981 gaatctggta agggtccgcc tcccaccacg gaggaggagg cctccggccc cccaggagag
     5041 ccccggctgg acagtgagac agagagtgac catgatgatg ccttcctctc catcatgtct
     5101 cctgagatcc agttgcctct accgcccgga aaacgtcgga cccagtccct cagtgcccta
     5161 cccaaggaac gggactcatc ttctgagaag gatggacgca gccccaacaa gcgggagaag
     5221 gaccacatcc ggcggcccat gaatgccttc atgatcttca gcaagcggca ccgggccctg
     5281 gtccaccagc gtcatcccaa ccaggacaac cggaccgtca gcaagatcct gggcgagtgg
     5341 tggtatgccc tggggcccaa ggagaagcag aagtaccacg acctggcctt ccaggtgaag
     5401 gaggcccact tcaaggccca cccagattgg aagtggtgca acaaggaccg aaagaagtcc
     5461 agctcagagg ccaagcccac gagcctgggg ctggcaggag ggcacaagga gacgcgggag
     5521 cggagcatgt cggagacggg cactgctgct gcccctgggg tgtcctctga gctcctgtcc
     5581 gttgcagccc agacactcct gagctcagac accaaggctc cggggagcag ctcctgtggg
     5641 gcagaacggc tacacacagt tgggggacct ggctcagccc ggccccgagc tttctcccac
     5701 agcggggtac acagcctgga cggcggagaa gtagacagtc aggcgctaca ggaactgacg
     5761 cagatggtgt ctggccctgc atcgtactct ggcccaaagc cttctaccca gtatggagct
     5821 ccaggaccct ttgcagcccc tggtgaggga ggtgccttgg cggccactgg gcggcccccg
     5881 ctgctgccca cccgagcttc tcgttctcag cgtgcggcca gtgaggacat gacgagtgat
     5941 gaggagcgca tggtcatctg tgaggaggaa ggggatgatg atgtcattgc tgacgatggc
     6001 ttcggcacca ctgacattga tctcaagtgc aaggagcggg tgaccgacag cgagagtggg
     6061 gacagctctg gggaggaccc agagggcaac aagggctttg gtcggaaggt gttttcacct
     6121 gtgatccgtt cctcctttac ccactgccgc cccccactgg accctgagcc cccagggccc
     6181 ccggatcctc ctgtagcctt tggcaaaggc tatggttccg ccccatcctc ctctgcgtcc
     6241 tcgcctgctt cctcctcagc ctcggcagcc acctccttct cactgggctc aggaaccttc
     6301 aaggcccagg agtctggtca gggcagcaca gcgggccccc tacggccccc accccctggg
     6361 gctgggggtc cagcgacacc ttccaaggca acccggttcc tcccaatgga tcctgccacc
     6421 ttccggcgca agagacccga aagtgtgggt ggcctggagc caccaggccc ctcagtcatc
     6481 gcggcccctc ccagcggagg aggaaacatc ctgcagacac tggtgctgcc cccaaacaag
     6541 gaggagcaag agggcggcgg agccagagtg ccctccgccc ccgccccatc actggcctat
     6601 ggggccccag cagctcccct gtcccgtcct gccgccacca tggtcaccaa tgtggtgcgg
     6661 cctgtcagca gcactcctgt gcccatcgcc tctaagccct tccccacctc tggccgggct
     6721 gaggcgtctc caaatgacac agcaggtgcc aggactgaaa tgggcactgg gtctcgggtg
     6781 cctgggggct ccccgctggg tgtcagctta gtgtattcgg acaagaagtc ggcagcagcc
     6841 acctcaccag ccccacactt ggtggctgga cccctgctgg gcactgtggg gaaggcgcct
     6901 gccactgtca ctaacctact ggtgggcacc ccggggtatg gggcccctgc gccccctgct
     6961 gtccagttca ttgcccaggg ggcccctggt ggtgggacca ctgcgggctc aggagcaggt
     7021 gctgggagtg gccccaatgg gccagtaccc ctgggcatcc tgcaaccagg tgccctgggc
     7081 aaggctgggg gaatcaccca ggtacagtac atcctgccca cgctgcccca gcagcttcag
     7141 gtggcacctg ccccagcacc agcccctggg accaaggcag cggctcccag cggccctgca
     7201 cccaccacca gcatccgttt caccctccca ccgggcactt ccaccaacgg caaagtcctg
     7261 gctgccactg cacccactcc tggcatcccc atcctgcagt ctgtaccctc cgccccaccc
     7321 cccaaagccc agtcagtttc tcccgtgcag gccccgcccc cgggtggctc agcccagctg
     7381 ctgcctggga aggtcctagt gcctctggcc gcccctagca tgtcagtgcg gggtggaggg
     7441 gccggccagc cactgccact ggtgagcccg cccttctcag tacctgtgca gaatggtgcc
     7501 cagcccccca gcaagatcat ccagctgacc ccggtgcctg tgagcacacc cagcggcctg
     7561 gtgccgcccc tgagcccagc cacactccct ggacccacct ctcagcctca gaaggtcctg
     7621 ttgccctcct ccaccagaat cacctatgtg cagtcagcgg gcgggcacgc gctgcccctg
     7681 ggtaccagcc ctgcgtccag ccaggctgga acagtcacct cgtacgggcc cacgagctct
     7741 gtagctctag gcttcacctc gctggggccc agcggccccg ccttcgtgca gcccctgctc
     7801 tcagcaggcc aagccccact gctggctccc ggtcaggtgg gcgtgtcacc tgtgcccagt
     7861 ccccagctgc cgcctgcctg tgcagccccc ggaggtcctg tcataacagc attttactct
     7921 ggcagccctg cacccacctc ctcagcaccc ctggcccagc catcccaggc ccccccaagc
     7981 ctggtctaca ctgtggccac cagcacaacc ccacctgcag ccaccattct gcccaagggc
     8041 ccgccagccc ctgccactgc caccccagcc ccgactagcc ctttccccag cgccacagca
     8101 ggttccatga cctacagctt agtggccccc aaggcccagc ggcccagccc gaaggccccc
     8161 cagaaagtga aggcagccat cgccagcatt cccgtggggt cctttgaggc aggtgcctct
     8221 gggcggcctg gccctgcacc ccggcagcct ctggagcctg gcccagtccg agagccaact
     8281 gccccagagt ctgagcttga ggggcagccc acaccaccag cccctccacc cctgccagag
     8341 acctggactc ccacggcccg gagcagcccc ccactgcccc cacctgctga ggagcggacc
     8401 agcgccaagg gccctgagac catggccagc aaattcccca gctcatcttc agactggcgc
     8461 gtccctgggc agggcctgga gaatcgtggg gagcctccca ctcctcccag cccggcccca
     8521 gctccagctg tagcccctgg tggcagcagc gagagcagca gtgggcgggc agccggggac
     8581 accccggagc gcaaggaggc ggctggtact ggcaagaagg tgaaggtgcg gcccccgccc
     8641 ctgaagaaga cctttgactc tgtggacaac agggtcctgt cagaagtgga cttcgaagag
     8701 cgctttgctg agttgcctga gtttcggcct gaggaggtgc tgccctcccc caccctgcag
     8761 tctctggcca cctcaccccg ggccatcctg ggctcttacc gcaagaagag gaagaactcc
     8821 acggacctgg attcagcacc cgaggacccc acctcgccca agcgcaagat gagaagacgc
     8881 tccagctgca gctcggagcc caacaccccc aagagtgcca agtgcgaggg ggacatcttc
     8941 acctttgacc gtacaggtac agaagccgag gacgtgcttg gggagctaga gtatgacaag
     9001 gtgccatact cctccctgcg gcgcaccctg gaccagcgcc gggccctggt catgcagctc
     9061 tttcaggacc atggcttctt cccgtcagcc caggccacag ccgccttcca ggcccgctat
     9121 gcagacatct ttccctccaa ggtttgtctg cagttgaaga tccgtgaggt gcgccagaag
     9181 atcatgcagg ctgccactcc cacggagcag ccccctggag ctgaggctcc tctccctgta
     9241 ccgcccccca ctggcaccgc tgctgcccct gcccccactc ccagccccgc agggggccct
     9301 gaccccacct cacccagctc ggactctggc acggcccagg ctgccccgcc actgcctcca
     9361 cccccagagt cggggcctgg acagcctggc tgggaggggg ctccccagcc ctccccccca
     9421 cccccaggtc cctccacagc tgccacaggc ggaatctctg atgcaaatga ccatgatcta
     9481 cgtgagcctt ctggtatcaa gaatcctgga ggagccagcg cctctgcgag ggtttcatcc
     9541 tgggattctc agtcatatga cattgaacag atatgtctgg gggcttcaaa tcctccttgg
     9601 gcctccactc tctttgaaat agatgaattt gtaaagatct atgacttgcc aggggaagat
     9661 gacaccagca gcctaaatca atatcttttt ccagtatgcc ttgagtatga ccagctccaa
     9721 tcttcagtgt aaaacccagc tttcttgtac aaagtggtta ccggttagta atgaactagt
     9781 taagtcgaca atcaacgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     9841 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     9901 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     9961 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
    10021 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
    10081 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
    10141 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
    10201 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
    10261 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
    10321 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
    10381 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
    10441 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
    10501 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
    10561 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    10621 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    10681 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
    10741 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
    10801 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    10861 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
    10921 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
    10981 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    11041 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
    11101 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
    11161 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
    11221 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
    11281 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
    11341 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
    11401 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
    11461 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
    11521 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
    11581 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
    11641 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
    11701 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
    11761 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    11821 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
    11881 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
    11941 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
    12001 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
    12061 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
    12121 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
    12181 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
    12241 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    12301 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    12361 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
    12421 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    12481 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    12541 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    12601 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    12661 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    12721 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    12781 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    12841 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
