LOCUS       plasmid                 8311 bp    DNA     circular SYN 20-JUL-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     intron          join(7990..8311,1..617)
                     /note="pLannotate"
                     /label="EF-1α intron A"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     CDS             742..1458
                     /note="pLannotate"
                     /label="EGFP"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     polyA_signal    3367..3591
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    5082..5203
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(7274..7378)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             3930..4952
                     /note="pLannotate"
                     /label="HygR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.7"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(6413..7273)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             1689..3230
                     /note="pLannotate"
                     /label="COIL (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="89.2"
                     /fragment="True"
                     /other="CDS"
     rep_origin      complement(5654..6242)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    3875..3922
                     /note="pLannotate"
                     /label="FRT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             3282..3323
                     /note="pLannotate"
                     /label="V5 tag"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(5300..5330)
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(5345..5366)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        7779..7989
                     /note="pLannotate"
                     /label="EF-1α core promoter"
                     /database="snapgene"
                     /identity="99.1"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        643..661
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    complement(5276..5292)
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      3637..3870
                     /note="pLannotate"
                     /label="f1 ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="65.2"
                     /fragment="True"
                     /other="rep_origin"
     CDS             4962..5087
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="97.6"
                     /match_length="33.3"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(7437..7489)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             7687..7753
                     /note="pLannotate"
                     /label="small t antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(5379..5471)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     promoter        3595..3616
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
     enhancer        7644..7662
                     /note="pLannotate"
                     /label="CMV enhancer (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.0"
                     /fragment="True"
                     /other="enhancer"
ORIGIN
        1 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
       61 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
      121 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
      181 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
      241 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
      301 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
      361 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
      421 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
      481 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
      541 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
      601 tttttcttcc atttcaggtg tcgtgaggaa ttagcttggt actaatacga ctcactatag
      661 ggagacccaa gctggctagg taagcttggt accgagctcg gatccactag tccagtgtgg
      721 tggaattctg cagatgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
      781 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      841 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      901 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
      961 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1021 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1081 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     1141 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     1201 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     1261 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     1321 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     1381 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     1441 atggacgagc tgtacaaggg tggaggtgga tctggtggag gtggatctgg tggcggcggt
     1501 tcaatggcag cttccgagac ggttaggcta cggcttcaat ttgattaccc gccgccagct
     1561 accccgcact gtacggcctt ctggcttctg gtcgacttga acagatgccg agtcgtcaca
     1621 gatctcatta gtctcatccg ccagcgcttc ggcttcagtt ctggggcctt cctaggcctc
     1681 tacctggagg ggggctcttg ccccccgccg agagcgcgcg ccttgtgaga gacaacgact
     1741 gcctcagagt taaattagaa gagagaggag ttgctgagaa ttctgtagtc atcagtaatg
     1801 gtgacattaa tttatctctt agaaaagcaa agaagcgggc atttcagtta gaggagggtg
     1861 aagaaactga accagattgc aaatattcaa agaagcattg gaagagtcga gagaacaata
     1921 acaataatga gaaggtcttg gatctggaac caaaagctgt cacagatcag actgtcagca
     1981 aaaaaaacaa gagaaaaaat aaagcaacct gtggcacagt gggtgatgat aacgaagagg
     2041 ccaaaagaaa atcaccaaag aaaaaggaga aatgtgaata taaaaaaaag gctaagaatc
     2101 ccaagtctcc gaaagtacag gcagtgaaag actgggccaa tcagagatgt agttctccaa
     2161 aaggttctgc tagaaacagc cttgttaaag ccaaaaggaa aggtagtgta agcgtttgct
     2221 caaaagagag tcccagttcc tcctcggagt ctgagtcttg tgatgaatct atcagtgatg
     2281 gtcccagcaa agtcactttg gaggccagaa attcctcaga gaaattacca actgagttat
     2341 caaaggaaga accctctacc aaaaatacaa ctgcagacaa actggctata aaacttggct
     2401 ttagccttac ccccagcaag ggcaagacct ctggaacaac atcttccagt tcagactcta
     2461 gtgcagagtc agacgaccaa tgcttgatgt catcgagcac cccggagtgt gctgcgggtt
     2521 tcttaaagac agtaggcctt tttgcaggaa gaggtcgtcc aggcccaggg ctgtcatcac
     2581 agactgcagg tgctgctgga tggaggcgtt ctggctcaaa tggtggtgga caggctcctg
     2641 gtgcttctcc cagtgtgtct ctccctgcta gtttaggaag aggatggggt agagaagaga
     2701 accttttttc ttggaaggga gctaagggac ggggcatgcg ggggagaggt cgaggacgag
     2761 ggcatcctgt ttcctgtgtt gtaaatagaa gcactgacaa ccagaggcaa cagcaattaa
     2821 atgacgtggt aaaaaattca tctactatta tccagaatcc agtagagaca cccaagaagg
     2881 actatagtct gttaccactg ttagcagctg cccctcaagt tggagaaaag attgcattta
     2941 agcttttgga gctaacatcc agttactctc ctgatgtctc tgactacaag gaaggaagaa
     3001 tattaagcca caatccagag acccagcaag tagatataga aattctttca tccttacctg
     3061 ccttgagaga acctgggaaa tttgatttag tttatcacaa tgaaaatgga gccgaggtag
     3121 tggagtacgc tgtgacacag gagagcaaga tcactgtatt ttggaaagag ttgattgacc
     3181 caagactgat tattgaatct ccaagtaaca catcaagtac agaacctgcc tgaatccagc
     3241 acagtggcgg ccgctcgagt ctagagggcc cgcggttcga aggtaagcct atccctaacc
     3301 ctctcctcgg tctcgattct acgcgtaccg gttagtaatg agtttaaacc cgctgatcag
     3361 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     3421 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     3481 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     3541 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     3601 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     3661 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3721 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3781 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3841 aaaaacttga ttagggtgat ggttcacgta cctagaagtt cctattccga agttcctatt
     3901 ctctagaaag tataggaact tccttggcca aaaagcctga actcaccgcg acgtctgtcg
     3961 agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg
     4021 aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata
     4081 gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc
     4141 tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc tattgcatct
     4201 cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc
     4261 tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg
     4321 ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat
     4381 gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg
     4441 cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc
     4501 ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa
     4561 cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca
     4621 tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga
     4681 ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg
     4741 accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc
     4801 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
     4861 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
     4921 gccccagcac tcgtccgagg gcaaaggaat agcacgtact acgagatttc gattccaccg
     4981 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     5041 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     5101 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5161 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     5221 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5281 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5341 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5401 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5461 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5521 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5581 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5641 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5701 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5761 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5821 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5881 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     5941 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6001 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6061 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     6121 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6181 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6241 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6301 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6361 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6421 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6481 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6541 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6601 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6661 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6721 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6781 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6841 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6901 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     6961 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7021 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7081 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7141 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7201 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7261 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7321 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7381 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     7441 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     7501 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     7561 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     7621 tgtacgggcc agatatacgc gttgacattg attattgact aggcttttgc aaaaagcttt
     7681 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     7741 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     7801 agtccccgag aagttggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     7861 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     7921 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     7981 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     8041 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt
     8101 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     8161 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     8221 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     8281 gcgacgcttt ttttctggca agatagtctt g
//
