LOCUS       plasmid                 7118 bp    DNA     circular SYN 07-AUG-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     intron          complement(join(6514..7118,1..334))
                     /note="pLannotate"
                     /label="EF-1α intron A"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     polyA_signal    complement(4733..4957)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(3121..3242)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        946..1050
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(3372..4394)
                     /note="pLannotate"
                     /label="HygR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.7"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(5094..5977)
                     /note="pLannotate"
                     /label="HaloTag® (fragment)"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="99.2"
                     /fragment="True"
                     /other="CDS"
     CDS             1051..1911
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      2082..2670
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    complement(4402..4449)
                     /note="pLannotate"
                     /label="FRT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(5001..5042)
                     /note="pLannotate"
                     /label="V5 tag"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        2994..3024
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    2958..2979
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(335..545)
                     /note="pLannotate"
                     /label="EF-1α core promoter"
                     /database="snapgene"
                     /identity="99.1"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(6470..6488)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    3032..3048
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      complement(4454..4687)
                     /note="pLannotate"
                     /label="f1 ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="65.2"
                     /fragment="True"
                     /other="rep_origin"
     CDS             complement(6044..6352)
                     /note="pLannotate"
                     /label="H4_BOVIN"
                     /database="swissprot"
                     /identity="96.1"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(3237..3362)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="97.6"
                     /match_length="33.3"
                     /fragment="True"
                     /other="CDS"
     misc_feature    835..887
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(571..637)
                     /note="pLannotate"
                     /label="small t antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.8"
                     /fragment="True"
                     /other="CDS"
     CDS             2853..2945
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(6364..6385)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="95.5"
                     /match_length="20.4"
                     /fragment="True"
                     /other="misc_feature"
     promoter        complement(4708..4729)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
     enhancer        complement(662..680)
                     /note="pLannotate"
                     /label="CMV enhancer (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.0"
                     /fragment="True"
                     /other="enhancer"
     promoter        complement(646..665)
                     /note="pLannotate"
                     /label="SRα promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.3"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 ggcccgcatt tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat
       61 tttaaatggc tagagactta tcgaaagcag cgagacaggc gcgaaggtgc caccagattc
      121 gcacgcggcg gccccagcgc ccaggccagg cctcaactca agcacgaggc gaaggggctc
      181 cttaagcgca aggcctcgaa ctctcccacc cacttccaac ccgaagctcg ggatcaagaa
      241 tcacgtactg cagccaggtg gaagtaattc aaggcacgca agggccataa cccgtaaaga
      301 ggccaggccc gcgggaacca cacacggcac ttacctgtgt tctggcggca aacccgttgc
      361 gaaaaagaac gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa
      421 aaaggcggag ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg
      481 caccggttca attgccgacc cctcccccaa cttctcgggg actgtgggcg atgtgcgctc
      541 tgcccactga cgggcaccgg agccaattcc cactcctttc aagacctaga aggtccatta
      601 gctgcaaaga ttcctctctg tttaaaactt tatccatctt tgcaaagctt tttgcaaaag
      661 cctagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa gcagcgcaaa
      721 acgcctaacc ctaagcagat tcttcatgca attgtcggtc aagccttgcc ttgttgtagc
      781 ttaaattttg ctcgcgcact actcagcgac ctccaacaca caagcaggga gcagatactg
      841 gcttaactat gcggcatcag agcagattgt actgagagtg caccataggg gatcgggaga
      901 tctcccgatc cgtcgacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      961 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1021 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1081 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1141 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1201 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1261 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1321 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1381 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1441 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1501 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1561 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1621 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1681 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1741 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1801 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     1861 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     1921 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     1981 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2041 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2101 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2161 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2221 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2281 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2341 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2401 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2461 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2521 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2581 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2641 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2701 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2761 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     2821 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     2881 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     2941 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     3001 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     3061 aacagctatg accatgatta cgccaagctc tagctagagg tcgacggtat acagacatga
     3121 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
     3181 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     3241 ttggggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg
     3301 tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct
     3361 cgtagtacgt gctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg
     3421 gcgagtactt ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta
     3481 cgcccgacag tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct
     3541 gcatcatcga aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc
     3601 atatacgccc ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc
     3661 gtctgctgct ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat
     3721 tgggaatccc cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc
     3781 gtcaggacat tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc
     3841 tcggcccaaa gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc
     3901 atcacagttt gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat
     3961 gtagtgtatt gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga
     4021 tcggccgcag cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg
     4081 gtttcaggca ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg
     4141 ctctcgctga attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag
     4201 tgccgataaa cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca
     4261 tatccacgcc ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc
     4321 atcaggtcgg agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg
     4381 agttcaggct ttttggccaa ggaagttcct atactttcta gagaatagga acttcggaat
     4441 aggaacttct aggtacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     4501 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     4561 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     4621 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     4681 ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag
     4741 cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc
     4801 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
     4861 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
     4921 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa
     4981 actcattact aaccggtacg cgtagaatcg agaccgagga gagggttagg gataggctta
     5041 ccttcgaacc gcgggccctc tagactcgag cggcggccgc cgaattaact taaccggaaa
     5101 tctccagagt agacagccag cgcgcgatct cgctgccgat caggtccggg ttgtcttctt
     5161 gcagcagatt cagacccggg ccgatgtcca cagccttgca gttaggcagg cttttggcca
     5221 ggcgagcggc ttcggccggt gggatcagaa cgcctggggt gccccagaac agcagcttcg
     5281 ggacagggga ctggtgcagc cagtccatgt attcttcgac cagcgcgacg atgttcgctg
     5341 gctcaccggc gattggcagc tcgtttggga agcgccacag tggctcgcgg tcaacaggat
     5401 tcaggaacgg ctcgcggtaa tggtccatct cgacttcagt cagcgggcgg acgacaccca
     5461 tcggcagcgt accctcgata aaaacgttct gatcgatgat cagcttgcgg ccgacgtcgg
     5521 tggtgcggaa ggcctggaag gtctcgcggg caaattctgg ccattcgtcc caggtcggga
     5581 tagggcggat gaactccata aatgcaatac ctttgacgcg ctctggattg cgcttggccc
     5641 agtggaaacc cagagcggag ccccagtcgt gaatgaccag gacgacctct tccagaccca
     5701 gggcttcgat gaaggcatcc atgaagcgga cgtggtcgtc gaagaaataa cccaggtctg
     5761 gtttgtcgga tttgcccata ccgatcaggt ctggagcaat gcagcgatgg gtcggtgcaa
     5821 catgcgggat gatgttgcgc cacacgtagg aggaggtcgg gttaccgtgc aggaacagca
     5881 caggggtgcc atcgcgcgga ccaacatcga cgtagtgcat gcgctcgccc aggacttcca
     5941 cataatgggg gtcgaatgga aagccagtac cgatttcgga accgccacca gagcctccac
     6001 ctccacttcc acctccgcct gaaccacctc caccagatgg atcaccgcca aagccataaa
     6061 gggtgcgtcc ctggcgctta agcgcgtaga ccacgtccat ggcagtgact gtcttgcgct
     6121 tggcgtgctc cgtataggtg acagcgtcac ggatcacgtt ctccaaaaac accttgagca
     6181 ccccgcgagt ctcctcgtag atcagaccag agatccgctt cacaccgcca cgccgggcca
     6241 gacgccggat ggccggcttg gtgatgccct ggatgttgtc acgcaacacc ttgcggtggc
     6301 gcgcggcacc ccccgcaccc aaacccgccc cgcccgcacc acgtccagac atctcgaaat
     6361 tcgctagctc tagagcggcc gccactgtgc tggatatctg cagaattcca ccacactgga
     6421 ctagtggatc cgagctcggt accaagctta cctagccagc ttgggtctcc ctatagtgag
     6481 tcgtattagt accaagctaa ttcctcacga cacctgaaat ggaagaaaaa aactttgaac
     6541 cactgtctga ggcttgagaa tgaaccaaga tccaaactca aaaagggcaa attccaagga
     6601 gaattacatc aagtgccaag ctggcctaac ttcagtctcc acccactcag tgtggggaaa
     6661 ctccatcgca taaaacccct ccccccaacc taaagacgac gtactccaaa agctcgagaa
     6721 ctaatcgagg tgcctggacg gcgcccggta ctccgtggag tcacatgaag cgacggctga
     6781 ggacggaaag gcccttttcc tttgtgtggg tgactcaccc gcccgctctc ccgagcgccg
     6841 cgtcctccat tttgagctcc ctgcagcagg gccgggaagc ggccatcttt ccgctcacgc
     6901 aactggtgcc gaccgggcca gccttgccgc ccagggcggg gcgatacacg gcggcgcgag
     6961 gccaggcacc agagcaggcc ggccagcttg agactacccc cgtccgattc tcggtggccg
     7021 cgctcgcagg ccccgcctcg ccgaacatgt gcgctgggac gcacgggccc cgtcgccgcc
     7081 cgcggcccca aaaaccgaaa taccagtgtg cagatctt
//
