LOCUS       plasmid                 7018 bp    DNA     circular SYN 03-AUG-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     promoter        complement(2737..3915)
                     /note="pLannotate"
                     /label="EF-1α promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(1066..1290)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(6472..6593)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        4297..4401
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(join(6723..7018,1..727))
                     /note="pLannotate"
                     /label="HygR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.7"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(1428..2311)
                     /note="pLannotate"
                     /label="HaloTag® (fragment)"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="99.2"
                     /fragment="True"
                     /other="CDS"
     CDS             4402..5262
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      5433..6021
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    complement(735..782)
                     /note="pLannotate"
                     /label="FRT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(1334..1375)
                     /note="pLannotate"
                     /label="V5 tag"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        6345..6375
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    6309..6330
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(2702..2720)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    6383..6399
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     rep_origin      complement(787..1020)
                     /note="pLannotate"
                     /label="f1 ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="65.2"
                     /fragment="True"
                     /other="rep_origin"
     CDS             complement(2378..2626)
                     /note="pLannotate"
                     /label="H4_BOVIN (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="80.6"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(6588..6713)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="97.6"
                     /match_length="33.3"
                     /fragment="True"
                     /other="CDS"
     misc_feature    4186..4238
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(3922..3988)
                     /note="pLannotate"
                     /label="small t antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.8"
                     /fragment="True"
                     /other="CDS"
     CDS             6204..6296
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     misc_feature    2644..2663
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.5"
                     /fragment="True"
                     /other="misc_feature"
     promoter        complement(1041..1062)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
     enhancer        complement(4013..4031)
                     /note="pLannotate"
                     /label="CMV enhancer (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.0"
                     /fragment="True"
                     /other="enhancer"
     promoter        complement(3997..4016)
                     /note="pLannotate"
                     /label="SRα promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.3"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
       61 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
      121 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
      181 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
      241 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
      301 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
      361 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
      421 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
      481 tgaattcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
      541 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
      601 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
      661 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
      721 gctttttggc caaggaagtt cctatacttt ctagagaata ggaacttcgg aataggaact
      781 tctaggtacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
      841 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
      901 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
      961 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     1021 ggggataccc cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg
     1081 catccccagc atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc
     1141 caccccccag aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt
     1201 aggaaaggac agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa
     1261 caacagatgg ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaactcatt
     1321 actaaccggt acgcgtagaa tcgagaccga ggagagggtt agggataggc ttaccttcga
     1381 accgcgggcc ctctagactc gagcggccgg ccgccgaatt aacttaaccg gaaatctcca
     1441 gagtagacag ccagcgcgcg atctcgctgc cgatcaggtc cgggttgtct tcttgcagca
     1501 gattcagacc cgggccgatg tccacagcct tgcagttagg caggcttttg gccaggcgag
     1561 cggcttcggc cggtgggatc agaacgcctg gggtgcccca gaacagcagc ttcgggacag
     1621 gggactggtg cagccagtcc atgtattctt cgaccagcgc gacgatgttc gctggctcac
     1681 cggcgattgg cagctcgttt gggaagcgcc acagtggctc gcggtcaaca ggattcagga
     1741 acggctcgcg gtaatggtcc atctcgactt cagtcagcgg gcggacgaca cccatcggca
     1801 gcgtaccctc gataaaaacg ttctgatcga tgatcagctt gcggccgacg tcggtggtgc
     1861 ggaaggcctg gaaggtctcg cgggcaaatt ctggccattc gtcccaggtc gggatagggc
     1921 ggatgaactc cataaatgca atacctttga cgcgctctgg attgcgcttg gcccagtgga
     1981 aacccagagc ggagccccag tcgtgaatga ccaggacgac ctcttccaga cccagggctt
     2041 cgatgaaggc atccatgaag cggacgtggt cgtcgaagaa ataacccagg tctggtttgt
     2101 cggatttgcc cataccgatc aggtctggag caatgcagcg atgggtcggt gcaacatgcg
     2161 ggatgatgtt gcgccacacg taggaggagg tcgggttacc gtgcaggaac agcacagggg
     2221 tgccatcgcg cggaccaaca tcgacgtagt gcatgcgctc gcccaggact tccacataat
     2281 gggggtcgaa tggaaagcca gtaccgattt cggaaccgcc accagagcct ccacctccac
     2341 ttccacctcc gcctgaacca cctccaccag atggatcacc gccaaagcca taaagggtgc
     2401 gtccctggcg cttaagcgcg tagaccacgt ccatggcagt gactgtcttg cgcttggcgt
     2461 gctccgtata ggtgacagcg tcacggatca cgttctccaa aaacaccttg agcaccccgc
     2521 gagtctcctc gtagatcaga ccagagatcc gcttcacacc gccacgccgg gccagacgcc
     2581 ggatggccgg cttggtgatg ccctggatgt tgtcacgcaa caccttcatc tcgaaattcg
     2641 ctagctctag aactagtgga tccgagctcg gtaccaagct tacctagcca gcttgggtct
     2701 ccctatagtg agtcgtatta gtaccaagct aattcctcac gacacctgaa atggaagaaa
     2761 aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact caaaaagggc
     2821 aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct ccacccactc
     2881 agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg acgtactcca
     2941 aaagctcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg agtcacatga
     3001 agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactcac ccgcccgctc
     3061 tcccgagcgc cgcgtcctcc attttgagct ccctgcagca gggccgggaa gcggccatct
     3121 ttccgctcac gcaactggtg ccgaccgggc cagccttgcc gcccagggcg gggcgataca
     3181 cggcggcgcg aggccaggca ccagagcagg ccggccagct tgagactacc cccgtccgat
     3241 tctcggtggc cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg acgcacgggc
     3301 cccgtcgccg cccgcggccc caaaaaccga aataccagtg tgcagatctt ggcccgcatt
     3361 tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat tttaaatggc
     3421 tagagactta tcgaaagcag cgagacaggc gcgaaggtgc caccagattc gcacgcggcg
     3481 gccccagcgc ccaggccagg cctcaactca agcacgaggc gaaggggctc cttaagcgca
     3541 aggcctcgaa ctctcccacc cacttccaac ccgaagctcg ggatcaagaa tcacgtactg
     3601 cagccaggtg gaagtaattc aaggcacgca agggccataa cccgtaaaga ggccaggccc
     3661 gcgggaacca cacacggcac ttacctgtgt tctggcggca aacccgttgc gaaaaagaac
     3721 gttcacggcg actactgcac ttatatacgg ttctccccca ccctcgggaa aaaggcggag
     3781 ccagtacacg acatcacttt cccagtttac cccgcgccac cttctctagg caccggttca
     3841 attgccgacc cctcccccca acttctcggg gactgtgggc gatgtgcgct ctgcccactg
     3901 acgggcaccg gagccaattc ccactccttt caagacctag aaggtccatt agctgcaaag
     3961 attcctctct gtttaaaact ttatccatct ttgcaaagct ttttgcaaaa gcctagtcaa
     4021 taatcaatgt caacgcgtat atctggcccg tacatcgcga agcagcgcaa aacgcctaac
     4081 cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag cttaaatttt
     4141 gctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact ggcttaacta
     4201 tgcggcatca gagcagattg tactgagagt gcaccatagg ggatcgggag atctcccgat
     4261 ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4321 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4381 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4441 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4501 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4561 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4621 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4681 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4741 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4801 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4861 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4921 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4981 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5041 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5101 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5161 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5221 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5281 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5341 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5401 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5461 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5521 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5581 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5641 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5701 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5761 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5821 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5881 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5941 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6001 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6061 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6121 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6181 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6241 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6301 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6361 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     6421 gaccatgatt acgccaagct ctagctagag gtcgacggta tacagacatg ataagataca
     6481 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     6541 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttggggtgg
     6601 gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc gtcccggaaa
     6661 acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc tcgtagtacg
     6721 tgctattcct ttgccctcgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
     6781 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
     6841 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
     6901 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
     6961 cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgct
//
