LOCUS       plasmid                 7117 bp    DNA     circular SYN 09-AUG-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     intron          complement(join(6776..7117,1..597))
                     /note="pLannotate"
                     /label="EF-1α intron A"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
     polyA_signal    complement(4996..5220)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     polyA_signal    complement(3384..3505)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        1209..1313
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(3635..4657)
                     /note="pLannotate"
                     /label="HygR"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="99.7"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(5354..6237)
                     /note="pLannotate"
                     /label="HaloTag® (fragment)"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="99.2"
                     /fragment="True"
                     /other="CDS"
     CDS             1314..2174
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      2345..2933
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     protein_bind    complement(4665..4712)
                     /note="pLannotate"
                     /label="FRT"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(5264..5305)
                     /note="pLannotate"
                     /label="V5 tag"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        3257..3287
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    3221..3242
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(598..808)
                     /note="pLannotate"
                     /label="EF-1α core promoter"
                     /database="snapgene"
                     /identity="99.1"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        complement(6732..6750)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    3295..3311
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             complement(6304..6612)
                     /note="pLannotate"
                     /label="H4_BOVIN"
                     /database="swissprot"
                     /identity="99.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(4717..4950)
                     /note="pLannotate"
                     /label="f1 ori (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="65.2"
                     /fragment="True"
                     /other="rep_origin"
     CDS             complement(3500..3625)
                     /note="pLannotate"
                     /label="ble (fragment)"
                     /database="swissprot"
                     /identity="97.6"
                     /match_length="33.3"
                     /fragment="True"
                     /other="CDS"
     misc_feature    1098..1150
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.6"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(834..900)
                     /note="pLannotate"
                     /label="small t antigen (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.8"
                     /fragment="True"
                     /other="CDS"
     CDS             3116..3208
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     promoter        complement(4971..4992)
                     /note="pLannotate"
                     /label="SV40 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.1"
                     /fragment="True"
                     /other="promoter"
     enhancer        complement(925..943)
                     /note="pLannotate"
                     /label="CMV enhancer (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.0"
                     /fragment="True"
                     /other="enhancer"
     promoter        complement(909..928)
                     /note="pLannotate"
                     /label="SRα promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="3.3"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 gctccctgca gcagggccgg gaagcggcca tctttccgct cacgcaactg gtgccgaccg
       61 ggccagcctt gccgcccagg gcggggcgat acacggcggc gcgaggccag gcaccagagc
      121 aggccggcca gcttgagact acccccgtcc gattctcggt ggccgcgctc gcaggccccg
      181 cctcgccgaa catgtgcgct gggacgcacg ggccccgtcg ccgcccgcgg ccccaaaaac
      241 cgaaatacca gtgtgcagat cttggcccgc atttacaaga ctatcttgcc agaaaaaaag
      301 cgtcgcagca ggtcatcaaa aattttaaat ggctagagac ttatcgaaag cagcgagaca
      361 ggcgcgaagg tgccaccaga ttcgcacgcg gcggccccag cgcccaggcc aggcctcaac
      421 tcaagcacga ggcgaagggg ctccttaagc gcaaggcctc gaactctccc acccacttcc
      481 aacccgaagc tcgggatcaa gaatcacgta ctgcagccag gtggaagtaa ttcaaggcac
      541 gcaagggcca taacccgtaa agaggccagg cccgcgggaa ccacacacgg cacttacctg
      601 tgttctggcg gcaaacccgt tgcgaaaaag aacgttcacg gcgactactg cacttatata
      661 cggttctccc ccaccctcgg gaaaaaggcg gagccagtac acgacatcac tttcccagtt
      721 taccccgcgc caccttctct aggcaccggt tcaattgccg acccctcccc caacttctcg
      781 gggactgtgg gcgatgtgcg ctctgcccac tgacgggcac cggagccaat tcccactcct
      841 ttcaagacct agaaggtcca ttagctgcaa agattcctct ctgtttaaaa ctttatccat
      901 ctttgcaaag ctttttgcaa aagcctagtc aataatcaat gtcaacgcgt atatctggcc
      961 cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg
     1021 gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc gacctccaac
     1081 acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     1141 gtgcaccata ggggatcggg agatctcccg atccgtcgac gtcaggtggc acttttcggg
     1201 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1261 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1321 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1381 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1441 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1501 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1561 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1621 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1681 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1741 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1801 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1861 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1921 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1981 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2041 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2101 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2161 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2221 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2281 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2341 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2401 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2461 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2521 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2581 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2641 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2701 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2761 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2821 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2881 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2941 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3001 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3061 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3121 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3181 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     3241 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     3301 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctctagctag
     3361 aggtcgacgg tatacagaca tgataagata cattgatgag tttggacaaa ccacaactag
     3421 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     3481 cattataagc tgcaataaac aagttggggt gggcgaagaa ctccagcatg agatccccgc
     3541 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga
     3601 aggcggcggt ggaatcgaaa tctcgtagta cgtgctattc ctttgccctc ggacgagtgc
     3661 tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg
     3721 cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt
     3781 cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga
     3841 gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct gcaagctccg
     3901 gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga
     3961 agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga
     4021 ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga
     4081 ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga
     4141 cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa
     4201 tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc
     4261 cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc tccgcgaccg
     4321 gctgcagaac agcgggcagt tcggtttcgg gcaggtcttg caacgtgaca ccctgtgcac
     4381 ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc acttccggaa
     4441 tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg
     4501 cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag
     4561 attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa
     4621 acttctcgac agacgtcgcg gtgagttcag gctttttggc caaggaagtt cctatacttt
     4681 ctagagaata ggaacttcgg aataggaact tctaggtacg tgaaccatca ccctaatcaa
     4741 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     4801 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     4861 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     4921 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     4981 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     5041 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     5101 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     5161 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     5221 tcgaggctga tcagcgggtt taaactcatt actaaccggt acgcgtagaa tcgagaccga
     5281 ggagagggtt agggataggc ttaccttcga accgcgggcc ctctagactc gagcggccgc
     5341 cgaattaact taaccggaaa tctccagagt agacagccag cgcgcgatct cgctgccgat
     5401 caggtccggg ttgtcttctt gcagcagatt cagacccggg ccgatgtcca cagccttgca
     5461 gttaggcagg cttttggcca ggcgagcggc ttcggccggt gggatcagaa cgcctggggt
     5521 gccccagaac agcagcttcg ggacagggga ctggtgcagc cagtccatgt attcttcgac
     5581 cagcgcgacg atgttcgctg gctcaccggc gattggcagc tcgtttggga agcgccacag
     5641 tggctcgcgg tcaacaggat tcaggaacgg ctcgcggtaa tggtccatct cgacttcagt
     5701 cagcgggcgg acgacaccca tcggcagcgt accctcgata aaaacgttct gatcgatgat
     5761 cagcttgcgg ccgacgtcgg tggtgcggaa ggcctggaag gtctcgcggg caaattctgg
     5821 ccattcgtcc caggtcggga tagggcggat gaactccata aatgcaatac ctttgacgcg
     5881 ctctggattg cgcttggccc agtggaaacc cagagcggag ccccagtcgt gaatgaccag
     5941 gacgacctct tccagaccca gggcttcgat gaaggcatcc atgaagcgga cgtggtcgtc
     6001 gaagaaataa cccaggtctg gtttgtcgga tttgcccata ccgatcaggt ctggagcaat
     6061 gcagcgatgg gtcggtgcaa catgcgggat gatgttgcgc cacacgtagg aggaggtcgg
     6121 gttaccgtgc aggaacagca caggggtgcc atcgcgcgga ccaacatcga cgtagtgcat
     6181 gcgctcgccc aggacttcca cataatgggg gtcgaatgga aagccagtac cgatttcgga
     6241 accgccacca gagcctccac ctccacttcc acctccgcct gaaccacctc caccagatgg
     6301 atcaccgcca aagccataaa gggtgcgtcc ctggcgctta agcgcgtaga ccacgtccat
     6361 ggcagtgact gtcttgcgct tggcgtgctc cgtataggtg acagcgtcac ggatcacgtt
     6421 ctccaaaaac accttgagca ccccgcgagt ctcctcgtag atcagaccag agatccgctt
     6481 cacaccgcca cgccgggcca gacgccggat ggccggcttg gtgatgccct ggatgttgtc
     6541 acgcaacacc ttgcggtggc gcctggcacc ccccttaccc aaacccttcc cgcccttacc
     6601 acgtccagac atctcgaaat tcgctagctc tagagcggcc ccgccactgt gctggatatc
     6661 tgcagaattc caccacactg gactagtgga tccgagctcg gtaccaagct tacctagcca
     6721 gcttgggtct ccctatagtg agtcgtatta gtaccaagct aattcctcac gacacctgaa
     6781 atggaagaaa aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact
     6841 caaaaagggc aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct
     6901 ccacccactc agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg
     6961 acgtactcca aaagctcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg
     7021 agtcacatga agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactcac
     7081 ccgcccgctc tcccgagcgc cgcgtcctcc attttga
//
