LOCUS       plasmid                 8050 bp    DNA     circular SYN 16-JUL-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     misc_feature    complement(6484..6946)
                     /note="pLannotate"
                     /label="IRES"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      1712..2140
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     LTR             complement(417..685)
                     /note="pLannotate"
                     /label="5' LTR (truncated)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
     promoter        complement(698..909)
                     /note="pLannotate"
                     /label="EF-1α core promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(5607..5728)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        2193..2297
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(7096..7979)
                     /note="pLannotate"
                     /label="HaloTag® (fragment)"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="99.2"
                     /fragment="True"
                     /other="CDS"
     CDS             2298..3158
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(5859..6458)
                     /note="pLannotate"
                     /label="PuroR"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      3329..3917
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(join(8046..8050,1..373))
                     /note="pLannotate"
                     /label="H2B"
                     /database="snapgene"
                     /identity="99.5"
                     /match_length="99.2"
                     /fragment="False"
                     /other="CDS"
     promoter        4241..4271
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    4205..4226
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     protein_bind    4279..4295
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             1552..1707
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4333..4800)
                     /note="pLannotate"
                     /label="B (fragment)"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="29.3"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(5737..5843)
                     /note="pLannotate"
                     /label="WPRE (fragment)"
                     /database="snapgene"
                     /identity="96.3"
                     /match_length="26.5"
                     /fragment="True"
                     /other="misc_feature"
     ncRNA           complement(5495..5587)
                     /note="pLannotate"
                     /label="G-CSF SLDE"
                     /database="Rfam"
                     /identity="100.0"
                     /match_length="96.9"
                     /fragment="False"
                     /other="ncRNA"
     CDS             4100..4192
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 cgctggtgta cttggtgatg gccttagtac cctcggacac ggcgtgcttg gccaactccc
       61 caggcagcag caggcgcacg gccgtctgga tctccctgga ggtgatggtc gagcgcttgt
      121 tgtaatgcgc caggcgggaa gcctcacctg cgatgcgctc gaaaatgtcg ttcacaaacg
      181 aattcatgat gcccatggcc ttggacgaaa tgccggtgtc agggtggacc tgcttcagaa
      241 ccttgtacac atagatggaa tagctctcct tgcggctgcg cttgcgcttc ttgccgcctt
      301 tcttctgcgc cttagtcacc gccttcttgg agcccttttt cggggcggga gcagacttcg
      361 ctggctctgg catctcgaaa ttcgctagct ctagagtagg cgccggtcac agcttggatc
      421 tgtaacggcg cagaacagaa aacgaaacaa agacgtagag ttgagcaagc agggtcaggc
      481 aaagcgtgga gagccggctg agtctaggta ggctccaagg gagcgccgga caaaggcccg
      541 gtctcgacct gagctttaaa cttacctaga cggcggacgc agttcaggag gcaccacagg
      601 cgggaggcgg cagaacgcga ctcaaccggc gtggatggcg gcctcaggta gggcggcggg
      661 cgcgtgaagg agagatgcga gcccctcgaa gcttcagctg tgttctggcg gcaaacccgt
      721 tgcgaaaaag aacgttcacg gcgactactg cacttatata cggttctccc ccaccctcgg
      781 gaaaaaggcg gagccagtac acgacatcac tttcccagtt taccccgcgc caccttctct
      841 aggcacccgt tcaattgccg acccctcccc ccaacttctc ggggactgtg ggcgatgtgc
      901 gctctgccca ctgacgggca ccggagcgat cgcagatcct tggccgtgga ggccttttcc
      961 ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca gaggaaagcg
     1021 atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct cggggatgcg
     1081 gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc tagcggggga
     1141 gggacgtaat tacatccctg ggggctttgg gggggggctg tccctcataa aagttttgtt
     1201 actttataga agaaattttg agtttttgtt ttttttaata aataaataaa cataaataaa
     1261 ttgtttgttg aatttattat tagtatgtaa gtgtaaatat aataaaactt aatatctatt
     1321 caaattaata aataaacctc gatatacaga ccgataaaac acatgcgtca attttacaca
     1381 tgattatctt taacgtacgt cacaatatga ttatctttct agggttaatc tagctgcgtg
     1441 ttctgcagcg tgtcgagcat cttcatctgc tccatcacgc tgtaaaacac atttgcaccg
     1501 cgagtctgcc cgtcctccac gggttcaaaa acgtgaatga acgaggcgcg ctcactggcc
     1561 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     1621 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     1681 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     1741 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     1801 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     1861 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     1921 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     1981 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     2041 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     2101 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     2161 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     2221 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     2281 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     2341 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     2401 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     2461 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     2521 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     2581 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     2641 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     2701 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     2761 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     2821 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     2881 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     2941 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     3001 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     3061 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     3121 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     3181 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     3241 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     3301 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     3361 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     3421 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     3481 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     3541 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     3601 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     3661 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     3721 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     3781 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     3841 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     3901 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     3961 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     4021 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     4081 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     4141 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     4201 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     4261 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     4321 atgattacgc caagcgcgcc cgccgggtaa ctcacggggt atccatgtcc atttctgcgg
     4381 catccagcca ggatacccgt cctcgctgac gtaatatccc agcgccgcac cgctgtcatt
     4441 aatctgcaca ccggcacggc agttccggct gtcgccggta ttgttcgggt tgctgatgcg
     4501 cttcgggctg accatccgga actgtgtccg gaaaagccgc gacgaactgg tatcccaggt
     4561 ggcctgaacg aacagttcac cgttaaaggc gtgcatggcc acaccttccc gaatcatcat
     4621 ggtaaacgtg cgttttcgct caacgtcaat gcagcagcag tcatcctcgg caaactcttt
     4681 ccatgccgct tcaacctcgc gggaaaaggc acgggcttct tcctccccga tgcccagata
     4741 gcgccagctt gggcgatgac tgagccggaa aaaagacccg acgatatgat cctgatgcag
     4801 ctagattaac cctagaaaga tagtctgcgt aaaattgacg catgcattct tgaaatattg
     4861 ctctctcttt ctaaatagcg cgaatccgtc gctgtgcatt taggacatct cagtcgccgc
     4921 ttggagctcc cgtgaggcgt gcttgtcaat gcggtaagtg tcactgattt tgaactataa
     4981 cgaccgcgtg agtcaaaatg acgcatgatt atcttttacg tgacttttaa gatttaactc
     5041 atacgataat tatattgtta tttcatgttc tacttacgtg ataacttatt atatatatat
     5101 tttcttgtta tagatatcag ggacagcccc cccccaaagc ccccagggat gtaattacgt
     5161 ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt ccggcgctcc
     5221 ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg
     5281 gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc tggggggata
     5341 cggggaaaaa gccataccaa tgggccctaa aaaaggaatc cagtcaattc cggggctaaa
     5401 cctggctgcc actgtttctt tagggacttc gttcctgtga ggacaggaaa ccctgcaacc
     5461 tggttgacca gagcaaggga tataagaacc cagtttggga acactgctgt ttaaatatta
     5521 aacagggatg tcttgtcccc cggaagggag accagatgag cttctgatag actaattgac
     5581 atcatttgag tcaattccag acatgataag atacattgat gagtttggac aaaccacaac
     5641 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     5701 aaccattata agctgcaata aacaagttta gttaacgcat gatacaaagg cattaaagca
     5761 gcgtatccac atagcgtaaa aggagcaaca tagttaagaa taccagtcaa tctttcacaa
     5821 attttgtaat ccagaggttg attgtcgaac gcgtcgactc aggcaccggg cttgcgggtc
     5881 atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg cggtgacggt gaagccgagc
     5941 cgctcgtaga aggggaggtt gcggggcgcg gaggtctcca ggaaggcggg caccccggcg
     6001 cgctcggccg cctccactcc ggggagcacg acggcgctgc ccagaccctt gccctggtgg
     6061 tcgggcgaga cgccgacggt ggccaggaac cacgcgggct ccttgggccg gtgcggcgcc
     6121 aggaggcctt ccatctgttg ctgcgcggcc agccgggaac cgctcaactc ggccatgcgc
     6181 gggccgatct cggcgaacac cgcccccgct tcgacgctct ccggcgtggt ccagaccgcc
     6241 accgcggcgc cgtcgtccgc gacccacacc ttgccgatgt cgagcccgac gcgcgtgagg
     6301 aagagttctt gcagctcggt gacccgctcg atgtggcggt ccggatcgac ggtgtggcgc
     6361 gtggcggggt agtcggcgaa cgcggcggcg agggtgcgta cggccctggg gacgtcgtcg
     6421 cgggtggcga ggcgcaccgt gggcttgtac tcggtcattc cggacgccat ggttgtggcc
     6481 atattatcat cgtgtttttc aaaggaaaac cacgtccccg tggttcgggg ggcctagacg
     6541 tttttttaac ctcgactaaa cacatgtaaa gcatgtgcac cgaggcccca gatcagatcc
     6601 catacaatgg ggtaccttct gggcatcctt cagccccttg ttgaatacgc ttgaggagag
     6661 ccatttgact ctttccacaa ctatccaact cacaacgtgg cactggggtt gtgccgcctt
     6721 tgcaggtgta tcttatacac gtggcttttg gccgcagagg cacctgtcgc caggtggggg
     6781 gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt cttcaagaag cttccagagg
     6841 aactgcttcc ttcacgacat tcaacagacc ttgcattcct ttggcgagag gggaaagacc
     6901 cctaggaatg ctcgtcaaga agacagggcc aggtttccgg gccctcacat tgccaaaaga
     6961 cggcaatatg gtggaaaata acatatagac aaacgcacac cggccttatt ccaagcggct
     7021 tcggccagta acgttagggg ggggggaggg agaggggcgc ggccgcggat ccgatttaaa
     7081 ttcgaattaa cttaaccgga aatctccaga gtagacagcc agcgcgcgat ctcgctgccg
     7141 atcaggtccg ggttgtcttc ttgcagcaga ttcagacccg ggccgatgtc cacagccttg
     7201 cagttaggca ggcttttggc caggcgagcg gcttcggccg gtgggatcag aacgcctggg
     7261 gtgccccaga acagcagctt cgggacaggg gactggtgca gccagtccat gtattcttcg
     7321 accagcgcga cgatgttcgc tggctcaccg gcgattggca gctcgtttgg gaagcgccac
     7381 agtggctcgc ggtcaacagg attcaggaac ggctcgcggt aatggtccat ctcgacttca
     7441 gtcagcgggc ggacgacacc catcggcagc gtaccctcga taaaaacgtt ctgatcgatg
     7501 atcagcttgc ggccgacgtc ggtggtgcgg aaggcctgga aggtctcgcg ggcaaattct
     7561 ggccattcgt cccaggtcgg gatagggcgg atgaactcca taaatgcaat acctttgacg
     7621 cgctctggat tgcgcttggc ccagtggaaa cccagagcgg agccccagtc gtgaatgacc
     7681 aggacgacct cttccagacc cagggcttcg atgaaggcat ccatgaagcg gacgtggtcg
     7741 tcgaagaaat aacccaggtc tggtttgtcg gatttgccca taccgatcag gtctggagca
     7801 atgcagcgat gggtcggtgc aacatgcggg atgatgttgc gccacacgta ggaggaggtc
     7861 gggttaccgt gcaggaacag cacaggggtg ccatcgcgcg gaccaacatc gacgtagtgc
     7921 atgcgctcgc ccaggacttc cacataatgg gggtcgaatg gaaagccagt accgatttcg
     7981 gaaccgccac cagagcctcc acctccactt ccacctccgc ctgaaccacc tccaccagat
     8041 ggatccttag
//
