LOCUS       pMS34_pgk_CSPG5        11436 bp    DNA     circular SYN 31-DEC-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11436)
  AUTHORS   Lim Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11436
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    68..7489
                     /label=match to pHR-CMVLacZ
                     /note="color: #a6acb3"
     polyA_site      2255..2386
                     /label=SV40 early poly A
                     /note="color: #80ff00"
     polyA_signal    2256..2447
                     /label=SV40 late polyA
                     /note="color: #ffc0cb; direction: RIGHT"
     polyA_site      2256..2386
                     /label=SV40 early poly A
                     /label=SV40 early poly A (2)
                     /note="color: #66ff66"
     polyA_site      2462..2480
                     /label=SV40 pA
                     /note="color: #ccff66"
     promoter        4160..4356
                     /label=SV40 early promoter
                     /note="color: #6495ed"
     promoter        4175..4501
                     /label=SV40 - Invitrogen promoter
                     /label=SV40 - Invitrogen
                     /note="color: #0000ff"
     rep_origin      4717..5399
                     /label=ColE1 origin
                     /note="color: #808080"
     CDS             complement(5494..6354)
                     /codon_start=1
                     /label=bla
                     /label=bla (AmpR)
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(5497..6156)
                     /label=AmpR
                     /note="color: #ffff00; direction: LEFT"
     primer_bind     7248..7265
                     /label=SP6
                     /note="color: #00ff00; direction: RIGHT"
     enhancer        7585..8176
                     /label=WPRE
                     /note="color: #a6acb3"
     primer_bind     complement(8102..8122)
                     /label=QB0112 WPRE-R binding site
                     /note="color: #00ffff; direction: LEFT"
     misc_feature    8130..8153
                     /label=cw11
                     /note="color: #00ff00; direction: RIGHT"
     misc_feature    8205..8212
                     /label=SbfI
                     /note="color: #ff0000"
     misc_feature    8244..8247
                     /label=Part D
                     /note="color: #00ffff"
     misc_feature    8252..8258
                     /label=AarI
                     /note="color: #ff0080"
     primer_bind     8256..8296
                     /label=Fragment 2.REV
                     /note="color: black; sequence: 
                     gtgggagttgcggccgcTCAtGAtCCgAG"
     primer_bind     8265..8279
                     /label=Fragment 1.REV
                     /note="color: black; sequence: gcggccgcaagtcaa"
     primer_bind     8266..8284
                     /label=pHR_BamHIhomo_rev
                     /note="color: black; sequence: cggccgcaagtcaaggatc"
     primer_bind     8268..8284
                     /label=PR102
                     /note="color: black; sequence: gccgcaagtcaaggatc"
     primer_bind     8269..8284
                     /label=Fragment 2.REV
                     /note="color: black; sequence: ccgcaagtcaaggatc"
     CDS             complement(8285..9898)
                     /codon_start=1
                     /label=Notch
                     /note="color: #000000"
                     /translation="ILDYSFTGGAGRDIPPPQIEEACELPECQVDAGNKVCNLQCNNHA
                     CGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQLTEGQCNP
                     LYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVLVVLLPPDQLRNNS
                     FHFLRELSHVLHTNVVFKRDAQGQQMIFPYYGHEEELRKHPIKRSTVGWATSSLLPGTS
                     GGRQRRELDPMDIRGSIVYLEIDNRQCVQSSSQCFQSATDVAAFLGALASLGSLNIPYK
                     IEAVKSEPVEPPLPSQLHLMYVAAAAFVLLFFVGCGVLLSRKRRRMKLLSSIEQACDIC
                     RLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLTRAHLTEVESRLERLEQLFLLIFP
                     REDLDMILKMDSLQDIKALLTGLFVQDNVNKDAVTDRLASVETDMPLTLRQHRISATSS
                     SEESSNKGQRQLTVSAAAGGSGGSGGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGS"
     misc_feature    8285..8920
                     /label=Gal4VP64
                     /note="color: #99cc00"
     CDS             complement(8405..8443)
                     /codon_start=1
                     /label=VP16
                     /note="color: #993366"
                     /translation="DALDDFDLDMLGS"
     CDS             complement(8444..8470)
                     /codon_start=1
                     /label=GS Linker
                     /note="color: #993366"
                     /translation="GGSGGSGGS"
     CDS             complement(8471..8920)
                     /codon_start=1
                     /label=Gal4DBD
                     /note="color: #993366"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSAAA"
     primer_bind     complement(8900..8921)
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: 
                     AAAGTTGATGAAGCTGCTGAGCAGCATC"
     misc_feature    8921..9898
                     /label=NL domains through cytoplasmic
                     /note="color: #a6acb3"
     primer_bind     complement(9876..9898)
                     /label=Notch_NL_fwd
                     /note="color: black; sequence: atcctggactacagcttcacagg"
     primer_bind     complement(9877..9898)
                     /label=Fragment 1.FOR
                     /note="color: black; sequence: atcctggactacagcttcacag"
     misc_feature    9899..9937
                     /label=VH constant
                     /note="color: #a6acb3"
     misc_feature    9938..9988
                     /label=H3 Nucl
                     /note="color: #a6acb3"
     misc_feature    9989..10105
                     /label=VH constant
                     /note="color: #a6acb3"
     misc_feature    10106..10135
                     /label=H2 Nucl
                     /note="color: #a6acb3"
     misc_feature    10136..10180
                     /label=VH constant
                     /note="color: #a6acb3"
     misc_feature    10181..10198
                     /label=H1 Nucl
                     /note="color: #a6acb3"
     misc_feature    10199..10282
                     /label=VH constant
                     /note="color: #a6acb3"
     misc_feature    10283..10330
                     /label=Linker
                     /note="color: #339966"
     misc_feature    10331..10363
                     /label=VL constant
                     /note="color: #a6acb3"
     misc_feature    10364..10384
                     /label=L3 Nucl
                     /note="color: #a6acb3"
     misc_feature    10385..10486
                     /label=VL constant
                     /note="color: #a6acb3"
     misc_feature    10487..10507
                     /label=L2 Nucl
                     /note="color: #a6acb3"
     misc_feature    10508..10558
                     /label=VL constant
                     /note="color: #c0c0c0"
     misc_feature    10559..10573
                     /label=L1 Nucl
                     /note="color: #a6acb3"
     misc_feature    10574..10654
                     /label=VL Constant
                     /note="color: #a6acb3"
     misc_feature    10655..10684
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /note="color: #ff00ff"
     CDS             complement(10685..10747)
                     /codon_start=1
                     /label=signal peptide
                     /note="color: #ffff00"
                     /translation="MALPVTALLLPLALLLHAARP"
     misc_feature    10748..10756
                     /label=KOZAK
                     /note="color: #a6acb3"
     primer_bind     complement(10757..10776)
                     /label=pHR_BamHIhomo_fwd
                     /note="color: black; sequence: AGGCAGGTGgacagtggatc"
     misc_feature    10768..10774
                     /label=AarI
                     /label=AarI(1)
                     /note="color: #ff0080; direction: RIGHT"
     misc_feature    10779..10782
                     /label=Part A
                     /note="color: #00ffff"
     primer_bind     10786..10819
                     /label=pHR_EcoRIhomo_pGK_rev
                     /note="color: black; sequence: 
                     TGacgcgtgagaattCGAAAGGCCCGGAGATGAG"
     promoter        complement(10801..11300)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    complement(10840..10858)
                     /label=QB0571
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(11286..11315)
                     /label=pHR_EcoRIhomo_pGK_fwd
                     /note="color: black; sequence: 
                     gcttgatatcgaattGGGTAGGGGAGGCGC"
     repeat_region   join(11324..11436,1..67)
                     /label=cPPT
                     /note="color: #a6acb3"
     primer_bind     complement(11325..11348)
                     /label=cPPT_5'seq
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(join(11423..11436,1..8))
                     /label=QB2741
                     /note="color: #ff0000; direction: LEFT"
ORIGIN
        1 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
       61 tttggcgacc gtcgagatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca
      121 ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga
      181 aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact
      241 attcttaaac ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa
      301 tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt
      361 tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa
      421 ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat
      481 ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa
      541 ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc
      601 tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc
      661 ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc
      721 agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa
      781 gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt
      841 gctactccta atggttcaat ttttactact ttatatttat ataattcact tctccaattg
      901 tccctcatat atatcgtcca ggtctgaaga tcaccggccg cttgctgtgc ggtggtctta
      961 cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt tatctctatc
     1021 ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag ttcttctgat
     1081 cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc tgatgtttct
     1141 aacaggccag gattaactgc gaatcgttct agctccctgc ttgcccatac tatatgtttt
     1201 aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg cgatctaatt
     1261 ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc tccgctagtc
     1321 aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca
     1381 agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa gtccctgttc
     1441 gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     1501 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     1561 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     1621 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
     1681 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag
     1741 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga
     1801 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca
     1861 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta
     1921 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct
     1981 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga
     2041 tcaaggatat cttgtcttct ttgggagtga attagccctt ccaactacta agtttgtagt
     2101 acatatttaa caaatacaat ttctttaaaa tgaaaataat tcagaggaat cacaggttta
     2161 gagtaaatga aaccactagg taattggcag tggtaatagg gtatggggtg ggaagtttgg
     2221 gatgattttg gttagcttga gttatccagt tgatccagac atgataagat acattgatga
     2281 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     2341 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     2401 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     2461 cctctacaaa tgtggtatgg ctgattatga tctctagtca aggcactata catcaaatat
     2521 tccttattaa cccctttaca aattaaaaag ctaaaggtac acaatttttg agcatagtta
     2581 ttaatagcag acactctatg cctgtgtgga gtaagaaaaa acagtatgtt atgattataa
     2641 ctgttatgcc tacttataaa ggttacagaa tatttttcca taattttctt gtatagcagt
     2701 gcagcttttt cctttgtggt gtaaatagca aagcaagcaa gagttctatt actaaacaca
     2761 gcatgactca aaaaacttag caattctgaa ggaaagtcct tggggtcttc tacctttctc
     2821 ttcttttttg gaggagtaga atgttgagag tcagcagtag cctcatcatc actagatggc
     2881 atttcttctg agcaaaacag gttttcctca ttaaaggcat tccaccactg ctcccattca
     2941 tcagttccat aggttggaat ctaaaataca caaacaatta gaatcagtag tttaacacat
     3001 tatacactta aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc
     3061 aattatgtca caccacagaa gtaaggttcc ttcacaaaga tccggggccc actcataaat
     3121 ccagttgccg ccacggtagc caatcaccgt atcgtataaa tcatcgtcgg tacgttcggc
     3181 atcgctcatc acaatacgtg cctggacgtc gaggatttcg cgtgggtcaa tgccgcgcca
     3241 gatccacatc agacggttaa tcatgcgata ccagtgaggg atggttttac catcaagggc
     3301 cgactgcaca ggcggttgtg cgccgtgatt aaagcggcgg actagcgtcg aggtttcagg
     3361 atgtttaaag cggggtttga acagggtttc gctcaggttt gcctgtgtca tggatgcagc
     3421 ctccagaata cttactggaa actattgtaa cccgcctgaa gttaaaaaga acaacgcccg
     3481 gcagtgccag gcgttgaaaa gattagcgac cggagattgg cgggacgaat acgacgccca
     3541 tatcccacgg ctgttcaatc caggtatctt gcgggatatc aacaacatag tcatcaacca
     3601 gcggacgacc agccggtttt gcgaagatgg tgacaaagtg cgcttttgga tacatttcac
     3661 gaatcgcaac cgcagtacca ccggtatcca ccaggtcatc aataacgatg aagccttcgc
     3721 catcgccttc tgcgcgtttc agcactttaa gctcgcgctg gttgtcgtga tcgtagctgg
     3781 aaatacaaac ggtatcgaca tgacgaatac ccagttcacg cgccagtaac gcacccggta
     3841 cagaccgcca cggcttacgg caataatgcc tttccattgt tcagaaggca tcagtcggct
     3901 tgcgagttta cgtgcatgga tctgcaacat gtcccaggtg acgatgtatt tttcgctcat
     3961 gtgaagtgtc ccagcctgtt tatctacggc ttaaaaagtg ttcgagggga aaataggttg
     4021 cgcgagatta tagagatctg gcgcactaaa aaccagtatt tcacatgagt ccgcgtcttt
     4081 ttacgcactg cctctccctg acgcgggata aagtggtatt ctcaaacata tctcgcaagc
     4141 ctgtcttgtg tccaagcttt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact
     4201 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc
     4261 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta
     4321 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg
     4381 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac
     4441 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct
     4501 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4561 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4621 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4681 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4741 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4801 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4861 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4921 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4981 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     5041 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     5101 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5161 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5221 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5281 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5341 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5401 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5461 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5521 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5581 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5641 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5701 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5761 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5821 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5881 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5941 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     6001 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     6061 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6121 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6181 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6241 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6301 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6361 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6421 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6481 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     6541 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     6601 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     6661 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagctaga
     6721 ttgtactgag agtgcaccat tcgacgctct cccttatgcg actcctgcat taggaagcag
     6781 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
     6841 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
     6901 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
     6961 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
     7021 tggctagcga tgaccctgct gattggttcg ctgaccattt ccgggtgcgg gacggcgtta
     7081 ccagaaactc agaaggttcg tccaaccaaa ccgactctga cggcagttta cgagagagat
     7141 gatagggtct gcttcagtaa gccagatgct acacaattag gcttgtacat attgtcgtta
     7201 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
     7261 tatagaatac acggaattaa ttctagatgc tgctagagat tttccacact gactaaaagg
     7321 gtctgaggga tctctagtta ccagagtcac acaacagacg ggcacacact acttgaagca
     7381 ctcaaggcaa gctttattga ggcttaagca gtgggttccc tagttagcca gagagctccc
     7441 aggctcagat ctggtctaac cagagagacc cagtacaagc aaaaagcaga tcttgtcttc
     7501 gttgggagtg aattagccct tccagtcccc ccttttcttt taaaaagtgg ctaagatcta
     7561 cagctgcctt ggtcgacggt atcgatgcgg ggaggcggcc caaagggaga tccgactcgt
     7621 ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg
     7681 aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca
     7741 ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca acaccacgga
     7801 attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag
     7861 ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt
     7921 ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg
     7981 cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat acaaggagga
     8041 gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc
     8101 acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta
     8161 atccagaggt tgattatcga taagcttgat atcaagcttg catgcctgca ggtcgactct
     8221 agagtcgcgg cctcagtcag tcacgcatgt tgcaggtggg agttgcggcc gcaagtcaag
     8281 gatctcatga tccgagcatg tccaggtcaa agtcatccag tgcatctgat cccaacatgt
     8341 cgagatcgaa atcgtccaga gcgtcagatc ccagcatgtc cagatcaaaa tcatccaggg
     8401 cgtcgctgcc cagcatatcc aggtcgaagt cgtccagggc atcagatccg ccagagcctc
     8461 cgcttccgcc tgcagcagca gacactgtca gctgccgctg gcccttgttg ctgctttcct
     8521 cgctgctgct ggtggcgctg attctgtgct gccgcagggt caggggcatg tcggtttcca
     8581 cgctggccag tctgtcggtc acggcgtcct tgttcacgtt gtcctgcacg aacaggccgg
     8641 tcagcagggc cttgatgtcc tgcaggctgt ccatcttcag gatcatgtcc aggtcctcgc
     8701 gtgggaagat cagcagaaac agctgttcca gtctttccag ccggctttcc acctcggtca
     8761 ggtgggctct ggtcaggggg cttctcttgg tcttggggct gtaccggcac tcccagttgt
     8821 tcttcaggca cttggcgcac ttgggctttt ctttgctgca cttcagtttc ttcagccggc
     8881 agatgtcaca ggcctgctcg atgctgctca gcagcttcat ccgccggcgc ttgcgggaca
     8941 gcagcacccc acagcccaca aagaacagga gcacgaaggc ggccgctgcc acgtacatga
     9001 ggtgcagctg cgagggcagc ggaggctcca ccggctcact cttcacggcc tcaatcttgt
     9061 aaggaatatt gaggctgcca agtgacgcaa gagcacctag gaaggcagcc acatcggtgg
     9121 cactctggaa gcactgcgag gatgactgca cacattgccg gttgtcgatc tccaggtaga
     9181 caatggagcc acggatgtcc atggggtcca gctccctgcg ctggcgccca ccactggtac
     9241 caggaagcag tgaagaggtg gcccaaccca ctgtagagcg cttgattggg tgcttgcgca
     9301 gctcttcctc gtggccatag tacgggaaga tcatctgctg gccttgcgca tcacgcttga
     9361 agaccacgtt ggtgtgcagc acgtggctga gctcccgcag aaagtggaag gagttgttcc
     9421 gtagctggtc gggtggaagc agcaccacca gcaccagggt gccggctgcc agccgctcgg
     9481 gtacatgctc agcacagtct aggccatccc actcacattc ggcactgtta cagccctggt
     9541 cgcagtggcc atcactgaag tggtccttgc agtactggtc atacaggggg ttgcactgtc
     9601 cctcggtgag ctggcagtcg aagccatcaa agaggcagcc ggccgagttg cactggctgt
     9661 cacagtggcc gtcgctaaaa tacttccagc actgtagaga ctgcgtgcag ttcttccagg
     9721 ggtcattgaa gttgagggag cagtcgccac catcccagcc acatgcgtga ttattacact
     9781 gcaggttgca gaccttattg cctgcatcca cctggcactc aggcagctca caggcctcct
     9841 caatctgcgg tgggggaatg tcgcgcccag cgccacctgt gaagctgtag tccaggatcg
     9901 aggagacggt gaccagggtt ccttgacccc agtagtcaaa agcagcagag aagtagtagt
     9961 aactccagta ccagtagtcg tagaagtagc gagcacaata atagacggca gtgtcctcag
    10021 ctcttaagct gttcatttgt aggtaggctg tgtttttgga tgtgtctgcg cttatagtga
    10081 aacggccctt gacgctatcg gcataagcag tagcgcctcc agaagcgtaa atatatgcaa
    10141 cccattccag gcccttaccc ggggcctgac gcacccagtg gatcgaataa gcagcgatat
    10201 cgaagccaga agctgcacag gacaaacgga gtgagccccc tggctgcacc aggccaccgc
    10261 cagactccac cagctgaacc tcggcaccac tgctactgcc accactacta ccactagcgg
    10321 cagtagtacc tttgatctcc accttggtac cctgtccgaa cgtgatcaga gaatgcggga
    10381 aagattgctg acagtaataa gttgcgaagt cttccggctg cagactgctg atggtcagag
    10441 tgaaatccgt cccggaacgg ctaccagaga agcgagaagg gactccagag tagagatcgg
    10501 atgccgagta aatcagaagc ttcggagctt ttcctggttt ctgttgatac caggctacag
    10561 cgctggacac ggactgactg gcacggcagg tgatggtgac cctatcgccc acagaggcgg
    10621 acagggagct cggggactgg gtcatctgga tatccagatc ctcttcgctg ataagttttt
    10681 gttcaggcct tgcagcgtgc agcagcagcg cgagtgggag caacagtgcc gtcacgggca
    10741 acgccatggt ggcggcgatc cactgtccac ctgccttgct ccacttgacg cgtgagaatt
    10801 cgaaaggccc ggagatgagg aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt
    10861 gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct
    10921 gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga aggagcaaag
    10981 ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg tgctgtccat
    11041 ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg
    11101 cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc
    11161 aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc
    11221 acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg
    11281 gaaaagcgcc tcccctaccc aattcgatat caagcttatc gatccaaact ggatctctgc
    11341 tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag
    11401 tttgtatgtc tgttgctatt atgtctacta ttcttt
//
