LOCUS       pMS35_pgk_BCAN_1       11400 bp    DNA     circular SYN 31-DEC-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11400)
  AUTHORS   Lim Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..113
                     /label=cPPT
                     /note="color: #a6acb3"
     primer_bind     1..14
                     /label=QB2741
                     /note="color: #ff0000; direction: RIGHT"
     primer_bind     89..112
                     /label=cPPT_5'seq
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     122..151
                     /label=pHR_EcoRIhomo_pGK_fwd
                     /note="color: black; sequence: 
                     gcttgatatcgaattGGGTAGGGGAGGCGC"
     promoter        137..636
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    579..597
                     /label=QB0571
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(618..651)
                     /label=pHR_EcoRIhomo_pGK_rev
                     /note="color: black; sequence: 
                     TGacgcgtgagaattCGAAAGGCCCGGAGATGAG"
     misc_feature    655..658
                     /label=Part A
                     /note="color: #00ffff"
     primer_bind     661..680
                     /label=pHR_BamHIhomo_fwd
                     /note="color: black; sequence: AGGCAGGTGgacagtggatc"
     misc_feature    complement(663..669)
                     /label=AarI
                     /label=AarI(1)
                     /note="color: #ff0080; direction: LEFT"
     misc_feature    681..689
                     /label=KOZAK
                     /note="color: #a6acb3"
     CDS             690..752
                     /codon_start=1
                     /label=signal peptide
                     /note="color: #ffff00"
                     /translation="MALPVTALLLPLALLLHAARP"
     misc_feature    753..782
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /note="color: #ff00ff"
     misc_feature    783..863
                     /label=VL Constant
                     /note="color: #a6acb3"
     misc_feature    864..878
                     /label=L1 Nucl
                     /note="color: #a6acb3"
     misc_feature    879..929
                     /label=VL constant
                     /note="color: #c0c0c0"
     misc_feature    930..950
                     /label=L2 Nucl
                     /note="color: #a6acb3"
     misc_feature    951..1052
                     /label=VL constant
                     /note="color: #a6acb3"
     misc_feature    1053..1067
                     /label=L3 Nucl
                     /note="color: #a6acb3"
     misc_feature    1068..1100
                     /label=VL constant
                     /note="color: #a6acb3"
     misc_feature    1101..1148
                     /label=Linker
                     /note="color: #339966"
     misc_feature    1149..1232
                     /label=VH constant
                     /note="color: #a6acb3"
     misc_feature    1233..1250
                     /label=H1 Nucl
                     /note="color: #a6acb3"
     misc_feature    1251..1295
                     /label=VH constant
                     /note="color: #a6acb3"
     misc_feature    1296..1325
                     /label=H2 Nucl
                     /note="color: #a6acb3"
     misc_feature    1326..1442
                     /label=VH constant
                     /note="color: #a6acb3"
     misc_feature    1443..1463
                     /label=H3 Nucl
                     /note="color: #a6acb3"
     misc_feature    1464..1502
                     /label=VH constant
                     /note="color: #a6acb3"
     CDS             1503..3116
                     /codon_start=1
                     /label=Notch
                     /note="color: #000000"
                     /translation="ILDYSFTGGAGRDIPPPQIEEACELPECQVDAGNKVCNLQCNNHA
                     CGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQLTEGQCNP
                     LYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVLVVLLPPDQLRNNS
                     FHFLRELSHVLHTNVVFKRDAQGQQMIFPYYGHEEELRKHPIKRSTVGWATSSLLPGTS
                     GGRQRRELDPMDIRGSIVYLEIDNRQCVQSSSQCFQSATDVAAFLGALASLGSLNIPYK
                     IEAVKSEPVEPPLPSQLHLMYVAAAAFVLLFFVGCGVLLSRKRRRMKLLSSIEQACDIC
                     RLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLTRAHLTEVESRLERLEQLFLLIFP
                     REDLDMILKMDSLQDIKALLTGLFVQDNVNKDAVTDRLASVETDMPLTLRQHRISATSS
                     SEESSNKGQRQLTVSAAAGGSGGSGGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGS"
     misc_feature    1503..2480
                     /label=NL domains through cytoplasmic
                     /note="color: #a6acb3"
     primer_bind     1503..1525
                     /label=Notch_NL_fwd
                     /note="color: black; sequence: atcctggactacagcttcacagg"
     primer_bind     1503..1524
                     /label=Fragment 1.FOR
                     /note="color: black; sequence: atcctggactacagcttcacag"
     primer_bind     2480..2501
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: 
                     AAAGTTGATGAAGCTGCTGAGCAGCATC"
     misc_feature    2481..3116
                     /label=Gal4VP64
                     /note="color: #99cc00"
     CDS             2481..2930
                     /codon_start=1
                     /label=Gal4DBD
                     /note="color: #993366"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSAAA"
     CDS             2931..2957
                     /codon_start=1
                     /label=GS Linker
                     /note="color: #993366"
                     /translation="GGSGGSGGS"
     CDS             2958..2996
                     /codon_start=1
                     /label=VP16
                     /note="color: #993366"
                     /translation="DALDDFDLDMLGS"
     primer_bind     complement(3105..3145)
                     /label=Fragment 2.REV
                     /note="color: black; sequence: 
                     gtgggagttgcggccgcTCAtGAtCCgAG"
     primer_bind     complement(3105..3145)
                     /label=Fragment 2.REVa
                     /note="color: black; sequence: 
                     gtgggagttgcggccgcTCAtGAtCCgAG"
     primer_bind     complement(3117..3135)
                     /label=pHR_BamHIhomo_rev
                     /note="color: black; sequence: cggccgcaagtcaaggatc"
     primer_bind     complement(3117..3133)
                     /label=PR102
                     /note="color: black; sequence: gccgcaagtcaaggatc"
     primer_bind     complement(3117..3132)
                     /label=Fragment 2.REV
                     /note="color: black; sequence: ccgcaagtcaaggatc"
     primer_bind     complement(3122..3136)
                     /label=Fragment 1.REV
                     /note="color: black; sequence: gcggccgcaagtcaa"
     misc_feature    3143..3149
                     /label=AarI
                     /note="color: #ff0080"
     misc_feature    3154..3157
                     /label=Part D
                     /note="color: #00ffff"
     misc_feature    3189..3196
                     /label=SbfI
                     /note="color: #ff0000"
     enhancer        3225..3816
                     /label=WPRE
                     /note="color: #a6acb3"
     misc_feature    complement(3248..3271)
                     /label=cw11
                     /note="color: #00ff00; direction: LEFT"
     primer_bind     3279..3299
                     /label=QB0112 WPRE-R binding site
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    3912..11333
                     /label=match to pHR-CMVLacZ
                     /note="color: #a6acb3"
     primer_bind     complement(4136..4153)
                     /label=SP6
                     /note="color: #00ff00; direction: LEFT"
     CDS             5047..5907
                     /codon_start=1
                     /label=bla
                     /label=bla (AmpR)
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             5245..5904
                     /label=AmpR
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      6002..6684
                     /label=ColE1 origin
                     /note="color: #808080"
     promoter        6900..7226
                     /label=SV40 - Invitrogen promoter
                     /label=SV40 - Invitrogen
                     /note="color: #0000ff"
     promoter        7045..7241
                     /label=SV40 early promoter
                     /note="color: #6495ed"
     polyA_site      8921..8939
                     /label=SV40 pA
                     /note="color: #ccff66"
     polyA_signal    complement(8954..9145)
                     /label=SV40 late polyA
                     /note="color: #ffc0cb; direction: LEFT"
     polyA_site      9015..9146
                     /label=SV40 early poly A
                     /note="color: #80ff00"
     polyA_site      9015..9145
                     /label=SV40 early poly A
                     /label=SV40 early poly A (2)
                     /note="color: #66ff66"
     repeat_region   11334..11400
                     /label=cPPT
                     /note="color: #a6acb3"
     primer_bind     11393..11400
                     /label=QB2741
                     /note="color: #ff0000; direction: RIGHT"
ORIGIN
        1 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
       61 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata
      121 agcttgatat cgaattgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
      181 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
      241 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
      301 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
      361 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
      421 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
      481 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
      541 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
      601 gcgctgttct cctcttcctc atctccgggc ctttcgaatt ctcacgcgtc aagtggagca
      661 aggcaggtgg acagtggatc gccgccacca tggcgttgcc cgtgacggca ctgttgctcc
      721 cactcgcgct gctgctgcac gctgcaaggc ctgaacaaaa acttatcagc gaagaggatc
      781 tggatatcca gatgacccag tccccgagct ccctgtccgc ctctgtgggc gatagggtca
      841 ccatcacctg ccgtgccagt cagtccgtgt ccagcgctgt agcctggtat caacagaaac
      901 caggaaaagc tccgaagctt ctgatttact cggcatccga tctctactct ggagtccctt
      961 ctcgcttctc tggtagccgt tccgggacgg atttcactct gaccatcagc agtctgcagc
     1021 cggaagactt cgcaacttat tactgtcagc aatacggtga cccgatcacg ttcggacagg
     1081 gtaccaaggt ggagatcaaa ggtactactg ccgctagtgg tagtagtggt ggcagtagca
     1141 gtggtgccga ggttcagctg gtggagtctg gcggtggcct ggtgcagcca gggggctcac
     1201 tccgtttgtc ctgtgcagct tctggcttcg atctcagtac ttattggatg cactgggtgc
     1261 gtcaggcccc gggtaagggc ctggaatggg ttgcaagtat ttacgcttct ggaggcgcta
     1321 ctgattatgc cgatagcgtc aagggccgtt tcactataag cgcagacaca tccaaaaaca
     1381 cagcctacct acaaatgaac agcttaagag ctgaggacac tgccgtctat tattgtgctc
     1441 gctactacca ttacccggct atggactact ggggtcaagg aaccctggtc accgtctcct
     1501 cgatcctgga ctacagcttc acaggtggcg ctgggcgcga cattccccca ccgcagattg
     1561 aggaggcctg tgagctgcct gagtgccagg tggatgcagg caataaggtc tgcaacctgc
     1621 agtgtaataa tcacgcatgt ggctgggatg gtggcgactg ctccctcaac ttcaatgacc
     1681 cctggaagaa ctgcacgcag tctctacagt gctggaagta ttttagcgac ggccactgtg
     1741 acagccagtg caactcggcc ggctgcctct ttgatggctt cgactgccag ctcaccgagg
     1801 gacagtgcaa ccccctgtat gaccagtact gcaaggacca cttcagtgat ggccactgcg
     1861 accagggctg taacagtgcc gaatgtgagt gggatggcct agactgtgct gagcatgtac
     1921 ccgagcggct ggcagccggc accctggtgc tggtggtgct gcttccaccc gaccagctac
     1981 ggaacaactc cttccacttt ctgcgggagc tcagccacgt gctgcacacc aacgtggtct
     2041 tcaagcgtga tgcgcaaggc cagcagatga tcttcccgta ctatggccac gaggaagagc
     2101 tgcgcaagca cccaatcaag cgctctacag tgggttgggc cacctcttca ctgcttcctg
     2161 gtaccagtgg tgggcgccag cgcagggagc tggaccccat ggacatccgt ggctccattg
     2221 tctacctgga gatcgacaac cggcaatgtg tgcagtcatc ctcgcagtgc ttccagagtg
     2281 ccaccgatgt ggctgccttc ctaggtgctc ttgcgtcact tggcagcctc aatattcctt
     2341 acaagattga ggccgtgaag agtgagccgg tggagcctcc gctgccctcg cagctgcacc
     2401 tcatgtacgt ggcagcggcc gccttcgtgc tcctgttctt tgtgggctgt ggggtgctgc
     2461 tgtcccgcaa gcgccggcgg atgaagctgc tgagcagcat cgagcaggcc tgtgacatct
     2521 gccggctgaa gaaactgaag tgcagcaaag aaaagcccaa gtgcgccaag tgcctgaaga
     2581 acaactggga gtgccggtac agccccaaga ccaagagaag ccccctgacc agagcccacc
     2641 tgaccgaggt ggaaagccgg ctggaaagac tggaacagct gtttctgctg atcttcccac
     2701 gcgaggacct ggacatgatc ctgaagatgg acagcctgca ggacatcaag gccctgctga
     2761 ccggcctgtt cgtgcaggac aacgtgaaca aggacgccgt gaccgacaga ctggccagcg
     2821 tggaaaccga catgcccctg accctgcggc agcacagaat cagcgccacc agcagcagcg
     2881 aggaaagcag caacaagggc cagcggcagc tgacagtgtc tgctgctgca ggcggaagcg
     2941 gaggctctgg cggatctgat gccctggacg acttcgacct ggatatgctg ggcagcgacg
     3001 ccctggatga ttttgatctg gacatgctgg gatctgacgc tctggacgat ttcgatctcg
     3061 acatgttggg atcagatgca ctggatgact ttgacctgga catgctcgga tcatgagatc
     3121 cttgacttgc ggccgcaact cccacctgca acatgcgtga ctgactgagg ccgcgactct
     3181 agagtcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat
     3241 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     3301 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     3361 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     3421 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3481 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3541 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3601 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     3661 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3721 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3781 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc aaggcagctg
     3841 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     3901 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     3961 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     4021 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     4081 ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata
     4141 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
     4201 taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc
     4261 atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg
     4321 taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca
     4381 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     4441 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     4501 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     4561 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     4621 ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa
     4681 tctagctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4741 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4801 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4861 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4921 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4981 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5041 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5101 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5161 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5221 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5281 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5341 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5401 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5461 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5521 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5581 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5641 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5701 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5761 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5821 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5881 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5941 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6001 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6061 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6121 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6181 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6241 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6301 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6361 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6421 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6481 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6541 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6601 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6661 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6721 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6781 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6841 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6901 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6961 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7021 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7081 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7141 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7201 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag
     7261 gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
     7321 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
     7381 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
     7441 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
     7501 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
     7561 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
     7621 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
     7681 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc
     7741 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
     7801 tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata
     7861 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
     7921 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
     7981 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
     8041 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
     8101 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
     8161 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
     8221 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
     8281 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     8341 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     8401 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     8461 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     8521 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     8581 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     8641 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     8701 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
     8761 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
     8821 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
     8881 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     8941 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     9001 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9061 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9121 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     9181 ccaaacttcc caccccatac cctattacca ctgccaatta cctagtggtt tcatttactc
     9241 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
     9301 actacaaact tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga
     9361 tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca caccagggcc
     9421 aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga
     9481 taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca
     9541 tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt
     9601 tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg
     9661 ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga
     9721 gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct
     9781 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     9841 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     9901 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     9961 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
    10021 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
    10081 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
    10141 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
    10201 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
    10261 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
    10321 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
    10381 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
    10441 taagaccacc gcacagcaag cggccggtga tcttcagacc tggacgatat atatgaggga
    10501 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
    10561 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
    10621 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
    10681 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
    10741 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
    10801 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
    10861 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
    10921 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
    10981 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
    11041 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    11101 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
    11161 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    11221 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
    11281 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtcgccaaa
    11341 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
//
