LOCUS       pMS36_pgk_GRM3_1       11439 bp    DNA     circular SYN 31-DEC-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11439)
  AUTHORS   Lim Lab
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11439
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     89..112
                     /label=cPPT_5'seq
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     122..151
                     /label=pHR_EcoRIhomo_pGK_fwd
                     /note="color: black; sequence: 
                     gcttgatatcgaattGGGTAGGGGAGGCGC"
     promoter        137..636
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    579..597
                     /label=QB0571
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(618..651)
                     /label=pHR_EcoRIhomo_pGK_rev
                     /note="color: black; sequence: 
                     TGacgcgtgagaattCGAAAGGCCCGGAGATGAG"
     misc_feature    655..658
                     /label=Part A
                     /note="color: #00ffff"
     primer_bind     661..680
                     /label=pHR_BamHIhomo_fwd
                     /note="color: black; sequence: AGGCAGGTGgacagtggatc"
     misc_feature    complement(663..669)
                     /label=AarI
                     /label=AarI(1)
                     /note="color: #ff0080; direction: LEFT"
     misc_feature    681..689
                     /label=KOZAK
                     /note="color: #a6acb3"
     CDS             690..752
                     /codon_start=1
                     /label=signal peptide
                     /note="color: #ffff00"
                     /translation="MALPVTALLLPLALLLHAARP"
     misc_feature    753..782
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /note="color: #ff00ff"
     misc_feature    783..863
                     /label=VL Constant
                     /note="color: #a6acb3"
     misc_feature    864..878
                     /label=L1 Nucl
                     /note="color: #a6acb3"
     misc_feature    879..929
                     /label=VL constant
                     /note="color: #c0c0c0"
     misc_feature    930..950
                     /label=L2 Nucl
                     /note="color: #a6acb3"
     misc_feature    951..1052
                     /label=VL constant
                     /note="color: #a6acb3"
     misc_feature    1053..1070
                     /label=L3 Nucl
                     /note="color: #a6acb3"
     misc_feature    1071..1103
                     /label=VL constant
                     /note="color: #a6acb3"
     misc_feature    1104..1151
                     /label=Linker
                     /note="color: #339966"
     misc_feature    1152..1235
                     /label=VH constant
                     /note="color: #a6acb3"
     misc_feature    1236..1253
                     /label=H1 Nucl
                     /note="color: #a6acb3"
     misc_feature    1254..1298
                     /label=VH constant
                     /note="color: #a6acb3"
     misc_feature    1299..1328
                     /label=H2 Nucl
                     /note="color: #a6acb3"
     misc_feature    1329..1445
                     /label=VH constant
                     /note="color: #a6acb3"
     misc_feature    1446..1502
                     /label=H3 Nucl
                     /note="color: #a6acb3"
     misc_feature    1503..1541
                     /label=VH constant
                     /note="color: #a6acb3"
     CDS             1542..3155
                     /codon_start=1
                     /label=Notch
                     /note="color: #000000"
                     /translation="ILDYSFTGGAGRDIPPPQIEEACELPECQVDAGNKVCNLQCNNHA
                     CGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQLTEGQCNP
                     LYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVLVVLLPPDQLRNNS
                     FHFLRELSHVLHTNVVFKRDAQGQQMIFPYYGHEEELRKHPIKRSTVGWATSSLLPGTS
                     GGRQRRELDPMDIRGSIVYLEIDNRQCVQSSSQCFQSATDVAAFLGALASLGSLNIPYK
                     IEAVKSEPVEPPLPSQLHLMYVAAAAFVLLFFVGCGVLLSRKRRRMKLLSSIEQACDIC
                     RLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLTRAHLTEVESRLERLEQLFLLIFP
                     REDLDMILKMDSLQDIKALLTGLFVQDNVNKDAVTDRLASVETDMPLTLRQHRISATSS
                     SEESSNKGQRQLTVSAAAGGSGGSGGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGS"
     misc_feature    1542..2519
                     /label=NL domains through cytoplasmic
                     /note="color: #a6acb3"
     primer_bind     1542..1564
                     /label=Notch_NL_fwd
                     /note="color: black; sequence: atcctggactacagcttcacagg"
     primer_bind     1542..1563
                     /label=Fragment 1.FOR
                     /note="color: black; sequence: atcctggactacagcttcacag"
     primer_bind     2519..2540
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: 
                     AAAGTTGATGAAGCTGCTGAGCAGCATC"
     misc_feature    2520..3155
                     /label=Gal4VP64
                     /note="color: #99cc00"
     CDS             2520..2969
                     /codon_start=1
                     /label=Gal4DBD
                     /note="color: #993366"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSAAA"
     CDS             2970..2996
                     /codon_start=1
                     /label=GS Linker
                     /note="color: #993366"
                     /translation="GGSGGSGGS"
     CDS             2997..3035
                     /codon_start=1
                     /label=VP16
                     /note="color: #993366"
                     /translation="DALDDFDLDMLGS"
     primer_bind     complement(3144..3184)
                     /label=Fragment 2.REV
                     /note="color: black; sequence: 
                     gtgggagttgcggccgcTCAtGAtCCgAG"
     primer_bind     complement(3156..3174)
                     /label=pHR_BamHIhomo_rev
                     /note="color: black; sequence: cggccgcaagtcaaggatc"
     primer_bind     complement(3156..3172)
                     /label=PR102
                     /note="color: black; sequence: gccgcaagtcaaggatc"
     primer_bind     complement(3156..3171)
                     /label=Fragment 2.REV
                     /note="color: black; sequence: ccgcaagtcaaggatc"
     primer_bind     complement(3161..3175)
                     /label=Fragment 1.REV
                     /note="color: black; sequence: gcggccgcaagtcaa"
     misc_feature    3182..3188
                     /label=AarI
                     /note="color: #ff0080"
     misc_feature    3193..3196
                     /label=Part D
                     /note="color: #00ffff"
     misc_feature    3228..3235
                     /label=SbfI
                     /note="color: #ff0000"
     enhancer        3264..3855
                     /label=WPRE
                     /note="color: #a6acb3"
     misc_feature    complement(3287..3310)
                     /label=cw11
                     /note="color: #00ff00; direction: LEFT"
     primer_bind     3318..3338
                     /label=QB0112 WPRE-R binding site
                     /note="color: #00ffff; direction: RIGHT"
     misc_feature    3951..11372
                     /label=match to pHR-CMVLacZ
                     /note="color: #a6acb3"
     primer_bind     complement(4175..4192)
                     /label=SP6
                     /note="color: #00ff00; direction: LEFT"
     CDS             5086..5946
                     /codon_start=1
                     /label=bla
                     /label=bla (AmpR)
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             5284..5943
                     /label=AmpR
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      6041..6723
                     /label=ColE1 origin
                     /note="color: #808080"
     promoter        6939..7265
                     /label=SV40 - Invitrogen promoter
                     /label=SV40 - Invitrogen
                     /note="color: #0000ff"
     promoter        7084..7280
                     /label=SV40 early promoter
                     /note="color: #6495ed"
     polyA_site      8960..8978
                     /label=SV40 pA
                     /note="color: #ccff66"
     polyA_signal    complement(8993..9184)
                     /label=SV40 late polyA
                     /note="color: #ffc0cb; direction: LEFT"
     polyA_site      9054..9185
                     /label=SV40 early poly A
                     /note="color: #80ff00"
     polyA_site      9054..9184
                     /label=SV40 early poly A
                     /label=SV40 early poly A (2)
                     /note="color: #66ff66"
     repeat_region   join(11373..11439,1..113)
                     /label=cPPT
                     /note="color: #a6acb3"
     primer_bind     join(11432..11439,1..14)
                     /label=QB2741
                     /note="color: #ff0000; direction: RIGHT"
ORIGIN
        1 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
       61 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata
      121 agcttgatat cgaattgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
      181 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
      241 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
      301 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
      361 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
      421 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
      481 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
      541 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
      601 gcgctgttct cctcttcctc atctccgggc ctttcgaatt ctcacgcgtc aagtggagca
      661 aggcaggtgg acagtggatc gccgccacca tggcgttgcc cgtgacggca ctgttgctcc
      721 cactcgcgct gctgctgcac gctgcaaggc ctgaacaaaa acttatcagc gaagaggatc
      781 tggatatcca gatgacccag tccccgagct ccctgtccgc ctctgtgggc gatagggtca
      841 ccatcacctg ccgtgccagt cagtccgtgt ccagcgctgt agcctggtat caacagaaac
      901 caggaaaagc tccgaagctt ctgatttact cggcatccga tctctactct ggagtccctt
      961 ctcgcttctc tggtagccgt tccgggacgg atttcactct gaccatcagc agtctgcagc
     1021 cggaagactt cgcaacttat tactgtcagc aatcttctta ttctctgatc acgttcggac
     1081 agggtaccaa ggtggagatc aaaggtacta ctgccgctag tggtagtagt ggtggcagta
     1141 gcagtggtgc cgaggttcag ctggtggagt ctggcggtgg cctggtgcag ccagggggct
     1201 cactccgttt gtcctgtgca gcttctggct tcgatatcag tagttattcg atgcactggg
     1261 tgcgtcaggc cccgggtaag ggcctggaat gggttgcacg tatttactct tctggaggca
     1321 ctactgctta tgccgatagc gtcaagggcc gtttcactat aagcgcagac acatccaaaa
     1381 acacagccta cctacaaatg aacagcttaa gagctgagga cactgccgtc tattattgtg
     1441 ctcgcggtca ttggttcttc cattactctt actacttcta cttcccgcgt tactacggtt
     1501 tggactactg gggtcaagga accctggtca ccgtctcctc gatcctggac tacagcttca
     1561 caggtggcgc tgggcgcgac attcccccac cgcagattga ggaggcctgt gagctgcctg
     1621 agtgccaggt ggatgcaggc aataaggtct gcaacctgca gtgtaataat cacgcatgtg
     1681 gctgggatgg tggcgactgc tccctcaact tcaatgaccc ctggaagaac tgcacgcagt
     1741 ctctacagtg ctggaagtat tttagcgacg gccactgtga cagccagtgc aactcggccg
     1801 gctgcctctt tgatggcttc gactgccagc tcaccgaggg acagtgcaac cccctgtatg
     1861 accagtactg caaggaccac ttcagtgatg gccactgcga ccagggctgt aacagtgccg
     1921 aatgtgagtg ggatggccta gactgtgctg agcatgtacc cgagcggctg gcagccggca
     1981 ccctggtgct ggtggtgctg cttccacccg accagctacg gaacaactcc ttccactttc
     2041 tgcgggagct cagccacgtg ctgcacacca acgtggtctt caagcgtgat gcgcaaggcc
     2101 agcagatgat cttcccgtac tatggccacg aggaagagct gcgcaagcac ccaatcaagc
     2161 gctctacagt gggttgggcc acctcttcac tgcttcctgg taccagtggt gggcgccagc
     2221 gcagggagct ggaccccatg gacatccgtg gctccattgt ctacctggag atcgacaacc
     2281 ggcaatgtgt gcagtcatcc tcgcagtgct tccagagtgc caccgatgtg gctgccttcc
     2341 taggtgctct tgcgtcactt ggcagcctca atattcctta caagattgag gccgtgaaga
     2401 gtgagccggt ggagcctccg ctgccctcgc agctgcacct catgtacgtg gcagcggccg
     2461 ccttcgtgct cctgttcttt gtgggctgtg gggtgctgct gtcccgcaag cgccggcgga
     2521 tgaagctgct gagcagcatc gagcaggcct gtgacatctg ccggctgaag aaactgaagt
     2581 gcagcaaaga aaagcccaag tgcgccaagt gcctgaagaa caactgggag tgccggtaca
     2641 gccccaagac caagagaagc cccctgacca gagcccacct gaccgaggtg gaaagccggc
     2701 tggaaagact ggaacagctg tttctgctga tcttcccacg cgaggacctg gacatgatcc
     2761 tgaagatgga cagcctgcag gacatcaagg ccctgctgac cggcctgttc gtgcaggaca
     2821 acgtgaacaa ggacgccgtg accgacagac tggccagcgt ggaaaccgac atgcccctga
     2881 ccctgcggca gcacagaatc agcgccacca gcagcagcga ggaaagcagc aacaagggcc
     2941 agcggcagct gacagtgtct gctgctgcag gcggaagcgg aggctctggc ggatctgatg
     3001 ccctggacga cttcgacctg gatatgctgg gcagcgacgc cctggatgat tttgatctgg
     3061 acatgctggg atctgacgct ctggacgatt tcgatctcga catgttggga tcagatgcac
     3121 tggatgactt tgacctggac atgctcggat catgagatcc ttgacttgcg gccgcaactc
     3181 ccacctgcaa catgcgtgac tgactgaggc cgcgactcta gagtcgacct gcaggcatgc
     3241 aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
     3301 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     3361 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     3421 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     3481 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     3541 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     3601 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     3661 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3721 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3781 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3841 gccgcctccc cgcatcgata ccgtcgacca aggcagctgt agatcttagc cactttttaa
     3901 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     3961 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4021 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4081 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4141 tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa atcgtatgtg
     4201 tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata tgtacaagcc
     4261 taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt aaactgccgt
     4321 cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc cgcacccgga
     4381 aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg ccggacgcat
     4441 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
     4501 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
     4561 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
     4621 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     4681 gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctagctctga tgccgcatag
     4741 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4801 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     4861 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     4921 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4981 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5041 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5101 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5161 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5221 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5281 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5341 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5401 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5461 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5521 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5581 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5641 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5701 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5761 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5821 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5881 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5941 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6001 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6061 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6121 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6181 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6241 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     6301 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6361 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6421 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6481 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6541 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6601 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6661 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6721 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6781 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6841 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     6901 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctgtggaat gtgtgtcagt
     6961 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7021 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     7081 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     7141 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7201 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7261 gaggcctagg cttttgcaaa aagcttggac acaagacagg cttgcgagat atgtttgaga
     7321 ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa
     7381 atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac
     7441 actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc
     7501 acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa
     7561 caatggaaag gcattattgc cgtaagccgt ggcggtctgt accgggtgcg ttactggcgc
     7621 gtgaactggg tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc
     7681 agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atcgttattg
     7741 atgacctggt ggataccggt ggtactgcgg ttgcgattcg tgaaatgtat ccaaaagcgc
     7801 actttgtcac catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg
     7861 atatcccgca agatacctgg attgaacagc cgtgggatat gggcgtcgta ttcgtcccgc
     7921 caatctccgg tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact
     7981 tcaggcgggt tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca
     8041 aacctgagcg aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc
     8101 cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg cccttgatgg taaaaccatc
     8161 cctcactggt atcgcatgat taaccgtctg atgtggatct ggcgcggcat tgacccacgc
     8221 gaaatcctcg acgtccaggc acgtattgtg atgagcgatg ccgaacgtac cgacgatgat
     8281 ttatacgata cggtgattgg ctaccgtggc ggcaactgga tttatgagtg ggccccggat
     8341 ctttgtgaag gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat
     8401 ttaaagctct aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct
     8461 aattgtttgt gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa
     8521 tgcctttaat gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc
     8581 tactgctgac tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa
     8641 ggactttcct tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct
     8701 tgcttgcttt gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat
     8761 ggaaaaatat tctgtaacct ttataagtag gcataacagt tataatcata acatactgtt
     8821 ttttcttact ccacacaggc atagagtgtc tgctattaat aactatgctc aaaaattgtg
     8881 tacctttagc tttttaattt gtaaaggggt taataaggaa tatttgatgt atagtgcctt
     8941 gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
     9001 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
     9061 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     9121 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     9181 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
     9241 ctattaccac tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa
     9301 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
     9361 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
     9421 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
     9481 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
     9541 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
     9601 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
     9661 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
     9721 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
     9781 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     9841 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     9901 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     9961 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
    10021 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
    10081 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
    10141 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
    10201 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
    10261 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
    10321 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
    10381 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
    10441 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
    10501 ggccggtgat cttcagacct ggacgatata tatgagggac aattggagaa gtgaattata
    10561 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    10621 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    10681 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    10741 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    10801 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    10861 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    10921 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    10981 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    11041 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    11101 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    11161 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    11221 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    11281 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
    11341 atccattcga ttagtgaacg gatctcgacg gtcgccaaat ggcagtattc atccacaatt
    11401 ttaaaagaaa aggggggatt ggggggtaca gtgcagggg
//
