LOCUS       pMS39_gb128_BCAN       11422 bp    DNA     circular SYN 31-DEC-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11422)
  AUTHORS   Milos S Simic
  TITLE     Direct Submission
  JOURNAL   Exported Sep 27, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    96..7517
                     /label=match to pHR-CMVLacZ
                     /note="color: #a6acb3"
     primer_bind     complement(320..337)
                     /label=SP6
                     /note="color: #00ff00; direction: LEFT"
     CDS             1231..2091
                     /codon_start=1
                     /label=bla
                     /label=bla (AmpR)
                     /note="color: #993366"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             1429..2088
                     /label=AmpR
                     /note="color: #ffff00; direction: RIGHT"
     rep_origin      2186..2868
                     /label=ColE1 origin
                     /note="color: #808080"
     promoter        3084..3410
                     /label=SV40 - Invitrogen promoter
                     /label=SV40 - Invitrogen
                     /note="color: #0000ff"
     promoter        3229..3425
                     /label=SV40 early promoter
                     /note="color: #6495ed"
     polyA_site      5105..5123
                     /label=SV40 pA
                     /note="color: #ccff66"
     polyA_signal    complement(5138..5329)
                     /label=SV40 late polyA
                     /note="color: #ffc0cb; direction: LEFT"
     polyA_site      5199..5330
                     /label=SV40 early poly A
                     /note="color: #80ff00"
     polyA_site      5199..5329
                     /label=SV40 early poly A
                     /label=SV40 early poly A (2)
                     /note="color: #66ff66"
     repeat_region   7518..7697
                     /label=cPPT
                     /note="color: #a6acb3"
     primer_bind     7577..7598
                     /label=QB2741
                     /note="color: #ff0000; direction: RIGHT"
     primer_bind     7673..7696
                     /label=cPPT_5'seq
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     7706..7735
                     /label=pHR_EcoRIhomo_pGK_fwd
                     /note="color: black; sequence: 
                     gcttgatatcgaattGGGTAGGGGAGGCGC"
     promoter        7721..8220
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    8163..8181
                     /label=QB0571
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     complement(8202..8235)
                     /label=pHR_EcoRIhomo_pGK_rev
                     /note="color: black; sequence: 
                     TGacgcgtgagaattCGAAAGGCCCGGAGATGAG"
     misc_feature    8239..8242
                     /label=Part A
                     /note="color: #00ffff"
     misc_feature    8245..8264
                     /label=BamHI pHR homology
                     /note="color: #00ffff"
     primer_bind     8245..8264
                     /label=pHR_BamHIhomo_fwd
                     /note="color: black; sequence: AGGCAGGTGgacagtggatc"
     misc_feature    complement(8247..8253)
                     /label=AarI
                     /label=AarI(1)
                     /note="color: #ff0080; direction: LEFT"
     misc_feature    8266..8274
                     /label=KOZAK
                     /note="color: #a6acb3"
     CDS             8275..8337
                     /codon_start=1
                     /label=signal peptide
                     /note="color: #ffff00"
                     /translation="MALPVTALLLPLALLLHAARP"
     misc_feature    8338..8367
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /label=Myc
                     /note="color: #ff00ff"
     misc_feature    8368..8448
                     /label=VL Constant
                     /note="color: #a6acb3"
     misc_feature    8449..8463
                     /label=L1 Nucl
                     /note="color: #a6acb3"
     misc_feature    8464..8514
                     /label=VL constant
                     /note="color: #c0c0c0"
     misc_feature    8515..8535
                     /label=L2 Nucl
                     /note="color: #a6acb3"
     misc_feature    8536..8637
                     /label=VL constant
                     /note="color: #a6acb3"
     misc_feature    8638..8652
                     /label=L3 Nucl
                     /note="color: #a6acb3"
     misc_feature    8653..8685
                     /label=VL constant
                     /note="color: #a6acb3"
     misc_feature    8686..8733
                     /label=Linker
                     /note="color: #339966"
     misc_feature    8734..8817
                     /label=VH constant
                     /note="color: #a6acb3"
     misc_feature    8818..8835
                     /label=H1 Nucl
                     /note="color: #a6acb3"
     misc_feature    8836..8880
                     /label=VH constant
                     /note="color: #a6acb3"
     misc_feature    8881..8910
                     /label=H2 Nucl
                     /note="color: #a6acb3"
     misc_feature    8911..9027
                     /label=VH constant
                     /note="color: #a6acb3"
     misc_feature    9028..9069
                     /label=H3
                     /note="color: #a6acb3"
     misc_feature    9070..9108
                     /label=VH constant
                     /note="color: #a6acb3"
     CDS             9109..10722
                     /codon_start=1
                     /label=Notch
                     /note="color: #000000"
                     /translation="ILDYSFTGGAGRDIPPPQIEEACELPECQVDAGNKVCNLQCNNHA
                     CGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQLTEGQCNP
                     LYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVLVVLLPPDQLRNNS
                     FHFLRELSHVLHTNVVFKRDAQGQQMIFPYYGHEEELRKHPIKRSTVGWATSSLLPGTS
                     GGRQRRELDPMDIRGSIVYLEIDNRQCVQSSSQCFQSATDVAAFLGALASLGSLNIPYK
                     IEAVKSEPVEPPLPSQLHLMYVAAAAFVLLFFVGCGVLLSRKRRRMKLLSSIEQACDIC
                     RLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLTRAHLTEVESRLERLEQLFLLIFP
                     REDLDMILKMDSLQDIKALLTGLFVQDNVNKDAVTDRLASVETDMPLTLRQHRISATSS
                     SEESSNKGQRQLTVSAAAGGSGGSGGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGS"
     misc_feature    9109..10086
                     /label=NL domains through cytoplasmic
                     /note="color: #a6acb3"
     primer_bind     9109..9131
                     /label=Notch_NL_fwd
                     /note="color: black; sequence: atcctggactacagcttcacagg"
     misc_feature    9109..9128
                     /label=Notch domain 20bp homology
                     /note="color: #666699"
     misc_feature    9109..9128
                     /label=hu3F8_scFv_gBlock
                     /note="IDT codon opt"
                     /note="color: #ffcc99"
     misc_feature    9254..9273
                     /label=Notch end Reverse Seq Primer
                     /note="color: #ccffcc"
     misc_feature    10025..10044
                     /label=Notch Middle Reverse Seq Primer
                     /note="color: #ff99cc"
     primer_bind     10086..10107
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: 
                     AAAGTTGATGAAGCTGCTGAGCAGCATC"
     misc_feature    10087..10722
                     /label=Gal4VP64
                     /note="color: #99cc00"
     misc_feature    10723..10742
                     /label=BamHI pHR homology for 3' of insert (JE)
                     /note="color: #a6acb3"
     primer_bind     complement(10723..10741)
                     /label=pHR_BamHIhomo_rev
                     /note="color: black; sequence: cggccgcaagtcaaggatc"
     misc_feature    10749..10755
                     /label=AarI
                     /note="color: #ff0080"
     misc_feature    10760..10763
                     /label=Part D
                     /note="color: #00ffff"
     misc_feature    10795..10802
                     /label=SbfI
                     /note="color: #ff0000"
     enhancer        10831..11422
                     /label=WPRE
                     /note="color: #a6acb3"
     misc_feature    complement(10854..10877)
                     /label=cw11
                     /note="color: #00ff00; direction: LEFT"
     primer_bind     10885..10905
                     /label=QB0112 WPRE-R binding site
                     /note="color: #00ffff; direction: RIGHT"
ORIGIN
        1 cgataccgtc gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
       61 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
      121 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      181 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      241 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
      301 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
      361 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
      421 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
      481 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
      541 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
      601 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      661 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      721 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
      781 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
      841 tcgaatggtg cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga
      901 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      961 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1021 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1441 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1741 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3001 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3061 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3181 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3241 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3421 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
     3481 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
     3541 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
     3601 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
     3661 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
     3721 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
     3781 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
     3841 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
     3901 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
     3961 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
     4021 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
     4081 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
     4141 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
     4201 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
     4261 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
     4321 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
     4381 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
     4441 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
     4501 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     4561 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     4621 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     4681 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     4741 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     4801 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     4861 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
     4921 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
     4981 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     5041 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
     5101 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5161 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     5341 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     5401 attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     5461 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     5521 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     5581 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     5641 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     5701 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     5761 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     5821 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     5881 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     5941 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6001 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6061 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6121 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6181 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6241 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6301 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     6361 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     6421 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     6481 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     6541 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     6601 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca
     6661 gacctggacg atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     6721 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     6781 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     6841 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     6901 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     6961 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7021 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7081 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7141 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7201 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7261 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7321 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7381 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     7441 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     7501 gaacggatct cgacggtcgc caaatggcag tattcatcca caattttaaa agaaaagggg
     7561 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     7621 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     7681 gcagagatcc agtttggatc gataagcttg atatcgaatt gggtagggga ggcgcttttc
     7741 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt
     7801 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt
     7861 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg
     7921 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag
     7981 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag
     8041 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc
     8101 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc
     8161 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg
     8221 aattctcacg cgtcaagtgg agcaaggcag gtggacagtg gatccgccgc caccatggcg
     8281 ttgcccgtga cggcactgtt gctcccactc gcgctgctgc tgcacgctgc aaggcctgaa
     8341 caaaaactta tcagcgaaga ggatctggat atccagatga cccagtcccc gagctccctg
     8401 tccgcctctg tgggcgatag ggtcaccatc acctgccgtg ccagtcagtc cgtgtccagc
     8461 gctgtagcct ggtatcaaca gaaaccagga aaagctccga agcttctgat ttactcggca
     8521 tccgatctct actctggagt cccttctcgc ttctctggta gccgttccgg gacggatttc
     8581 actctgacca tcagcagtct gcagccggaa gacttcgcaa cttattactg tcagcaattc
     8641 tactatctga taacgttcgg acagggtacc aaggtggaga tcaaaggtac tactgccgct
     8701 agtggtagta gtggtggcag tagcagtggt gccgaggttc agctggtgga gtctggcggt
     8761 ggcctggtgc agccaggggg ctcactccgt ttgtcctgtg cagcttctgg cttcgatatt
     8821 tcttcttatt ctatccactg ggtgcgtcag gccccgggta agggcctgga atgggttgca
     8881 ggcataagtt ctagcggggg agacacagca tatgccgata gcgtcaaggg ccgtttcact
     8941 ataagcgcag acacatccaa aaacacagcc tacctacaaa tgaacagctt aagagctgag
     9001 gacactgccg tctattattg tgctcgctat ttctacagtc atagtcggta tgcctactca
     9061 ggtgcgctcg actactgggg tcaaggaacc ctggtcaccg tctcctcgat cctggactac
     9121 agcttcacag gtggcgctgg gcgcgacatt cccccaccgc agattgagga ggcctgtgag
     9181 ctgcctgagt gccaggtgga tgcaggcaat aaggtctgca acctgcagtg taataatcac
     9241 gcatgtggct gggatggtgg cgactgctcc ctcaacttca atgacccctg gaagaactgc
     9301 acgcagtctc tacagtgctg gaagtatttt agcgacggcc actgtgacag ccagtgcaac
     9361 tcggccggct gcctctttga tggcttcgac tgccagctca ccgagggaca gtgcaacccc
     9421 ctgtatgacc agtactgcaa ggaccacttc agtgatggcc actgcgacca gggctgtaac
     9481 agtgccgaat gtgagtggga tggcctagac tgtgctgagc atgtacccga gcggctggca
     9541 gccggcaccc tggtgctggt ggtgctgctt ccacccgacc agctacggaa caactccttc
     9601 cactttctgc gggagctcag ccacgtgctg cacaccaacg tggtcttcaa gcgtgatgcg
     9661 caaggccagc agatgatctt cccgtactat ggccacgagg aagagctgcg caagcaccca
     9721 atcaagcgct ctacagtggg ttgggccacc tcttcactgc ttcctggtac cagtggtggg
     9781 cgccagcgca gggagctgga ccccatggac atccgtggct ccattgtcta cctggagatc
     9841 gacaaccggc aatgtgtgca gtcatcctcg cagtgcttcc agagtgccac cgatgtggct
     9901 gccttcctag gtgctcttgc gtcacttggc agcctcaata ttccttacaa gattgaggcc
     9961 gtgaagagtg agccggtgga gcctccgctg ccctcgcagc tgcacctcat gtacgtggca
    10021 gcggccgcct tcgtgctcct gttctttgtg ggctgtgggg tgctgctgtc ccgcaagcgc
    10081 cggcggatga agctgctgag cagcatcgag caggcctgtg acatctgccg gctgaagaaa
    10141 ctgaagtgca gcaaagaaaa gcccaagtgc gccaagtgcc tgaagaacaa ctgggagtgc
    10201 cggtacagcc ccaagaccaa gagaagcccc ctgaccagag cccacctgac cgaggtggaa
    10261 agccggctgg aaagactgga acagctgttt ctgctgatct tcccacgcga ggacctggac
    10321 atgatcctga agatggacag cctgcaggac atcaaggccc tgctgaccgg cctgttcgtg
    10381 caggacaacg tgaacaagga cgccgtgacc gacagactgg ccagcgtgga aaccgacatg
    10441 cccctgaccc tgcggcagca cagaatcagc gccaccagca gcagcgagga aagcagcaac
    10501 aagggccagc ggcagctgac agtgtctgct gctgcaggcg gaagcggagg ctctggcgga
    10561 tctgatgccc tggacgactt cgacctggat atgctgggca gcgacgccct ggatgatttt
    10621 gatctggaca tgctgggatc tgacgctctg gacgatttcg atctcgacat gttgggatca
    10681 gatgcactgg atgactttga cctggacatg ctcggatcat gagatccttg acttgcggcc
    10741 gcaactccca cctgcaacat gcgtgactga ctgaggccgc gactctagag tcgacctgca
    10801 ggcatgcaag cttgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
    10861 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
    10921 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
    10981 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
    11041 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
    11101 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
    11161 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
    11221 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
    11281 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
    11341 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
    11401 cctttgggcc gcctccccgc at
//
