LOCUS       Exported               12288 bp ds-DNA     circular SYN 02-JAN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    cTurboID-myc_pHygGate100
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12288)
  AUTHORS   AB
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 29, 2025 from SnapGene 3.2.1
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12288
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /note="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      105..357
                     /note="NOS terminator"
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(508..1533)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /note="HygR"
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(1585..1764)
                     /note="NOS promoter"
                     /note="nopaline synthase promoter"
     promoter        2205..2550
                     /note="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    2562..2681
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2711..2741
                     /note="lac UV5 promoter"
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2795..3454
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /note="CmR"
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             3796..4101
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /note="ccdB"
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(4142..4266)
                     /gene="mutant version of attR"
                     /bound_moiety="LR Clonase(TM)"
                     /note="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4284..5009
                     /note="cTurboID"
     CDS             5010..5039
                     /codon_start=1
                     /product="Myc (human c-Myc oncogene) epitope tag"
                     /note="Myc"
                     /translation="EQKLISEEDL"
     terminator      5059..5766
                     /note="OCS terminator"
                     /note="octopine synthase terminator"
     misc_feature    6010..6034
                     /note="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7334..7963
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /note="pVS1 StaA"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8392..9465
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /note="pVS1 RepA"
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9531..9725
                     /note="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    10069..10209
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(10395..10983)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11070..11864)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /note="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcga gctcgatcta gtaacataga
      121 tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta
      181 ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca
      241 ttacatgtta attattacat gcttaacgta attcaacaga aattatatga taatcatcgc
      301 aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg
      361 gaaattcggg ggatcgatcc cgcgaactgt ggacgagaac tgtgccacca agcgtaaggc
      421 cgttctctcg catttgcctt gctaggctcg cgcgagttgc tggctgaggc gttctcgaaa
      481 tcagctcttg ttcggtcggc atctactcta ttcctttgcc ctcggacgag tgctggggcg
      541 tcggtttcca ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct
      601 gcgggcgatt tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg
      661 accctgcgcc caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc
      721 aagaccaatg cggagcatat acgcccggag ccgcggcgat cctgcaagct ccggatgcct
      781 ccgctcgaag tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat
      841 gttggcgacc tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt
      901 tatgcggcca ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg
      961 gacttcgggg cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc
     1021 actgacggtg tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca
     1081 tatgaaatca cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc
     1141 gctcgtctgg ctaagatcgg ccgcagcgat cgcatccatg gcctccgcga ccggctgcag
     1201 aacagcgggc agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga
     1261 gatgcaatag gtcaggctct cgctgaatgc cccaatgtca agcacttccg gaatcgggag
     1321 cgcggccgat gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct
     1381 atttacccgc aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc
     1441 gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc
     1501 gacagacgtc gcggtgagtt caggcttttt catatcttat tgccccccgg gatcagatct
     1561 ggattgagag tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt
     1621 ggagcatttt tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg
     1681 cgcaataatg gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga
     1741 gtggctcctt caacgttgcg gttcaggcct acgcagcagg tctcatcaag acgatctacc
     1801 cgagtaacaa tctccaggag atcaaatacc ttcccaagaa ggttaaagat gcagtcaaaa
     1861 gattcaggac taattgcatc aagaacacag agaaagacat atttctcaag atcagaagta
     1921 ctattccagt atggacgatt caaggcttgc ttcataaacc aaggcaagta atagagattg
     1981 gagtctctaa aaaggtagtt cctactgaat ctaaggccat gcatggagtc taagattcaa
     2041 atcgaggatc taacagaact cgccgtgaag actggcgaac agttcataca gagtctttta
     2101 cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac tctggtctac
     2161 tccaaaaatg tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
     2221 aggataattt cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa
     2281 aggacagtag aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggct
     2341 atcattcaag atctctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
     2401 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgacatc
     2461 tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata
     2521 taaggaagtt catttcattt ggagaggaca cgctcgagat cacaagtttg tacaaaaaag
     2581 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
     2641 aacagactac ataatactgt aaaacacaac atatccagtc atattggcgg ccgcattagg
     2701 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     2761 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     2821 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     2881 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     2941 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     3001 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     3061 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     3121 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     3181 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     3241 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     3301 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     3361 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     3421 acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc
     3481 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     3541 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     3601 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     3661 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     3721 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     3781 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     3841 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     3901 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     3961 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     4021 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     4081 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     4141 ccatagtgac tggatatgtt gtgttttaca gcattatgta gtctgttttt tatgcaaaat
     4201 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     4261 agtggtgcct aggtgagtct agagggagcg tggcagtcct gcctgtggtc gactccacca
     4321 atcagtacct gctggatcga atcggcgagc tgaagagtgg ggatgcttgc attgcagaat
     4381 atcagcaggc agggagagga agcagaggga ggaaatggtt ctctcctttt ggagctaacc
     4441 tgtacctgag tatgttttgg cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg
     4501 tcatcggaat tgtcatggca gaagcgctgc gaaagctggg agcagacaag gtgcgagtca
     4561 aatggcccaa tgacctgtat ctgcaggata gaaagctggc aggcatcctg gtggagctgg
     4621 ccggaataac aggcgatgct gcacagatcg tcattggcgc cgggattaac gtggctatga
     4681 ggcgcgtgga ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa gcagggatta
     4741 acctggacag gaatactctg gccgctacgc tgatccgaga gctgcgggca gccctggaac
     4801 tgttcgagca ggaaggcctg gctccatatc tgccacggtg ggagaagctg gataacttca
     4861 tcaatagacc cgtgaagctg atcattgggg acaaagagat tttcgggatt agccggggga
     4921 ttgataaaca gggagccctg ctgctggaac aggacggagt tatcaaaccc tggatgggcg
     4981 gagaaatcag tctgcggtct gccgaaaagg agcaaaagct catttctgaa gaggacttgt
     5041 agactagtcc ctagagtcct gctttaatga gatatgcgag acgcctatga tcgcatgata
     5101 tttgctttca attctgttgt gcacgttgta aaaaacctga gcatgtgtag ctcagatcct
     5161 taccgccggt ttcggttcat tctaatgaat atatcacccg ttactatcgt atttttatga
     5221 ataatattct ccgttcaatt tactgattgt accctactac ttatatgtac aatattaaaa
     5281 tgaaaacaat atattgtgct gaataggttt atagcgacat ctatgataga gcgccacaat
     5341 aacaaacaat tgcgttttat tattacaaat ccaattttaa aaaaagcggc agaaccggtc
     5401 aaacctaaaa gactgattac ataaatctta ttcaaatttc aaaagtgccc caggggctag
     5461 tatctacgac acaccgagcg gcgaactaat aacgctcact gaagggaact ccggttcccc
     5521 gccggcgcgc atgggtgaga ttccttgaag ttgagtattg gccgtccgct ctaccgaaag
     5581 ttacgggcac cattcaaccc ggtccagcac ggcggccggg taaccgactt gctgccccga
     5641 gaattatgca gcattttttt ggtgtatgtg ggccccaaat gaagtgcagg tcaaaccttg
     5701 acagtgacga caaatcgttg ggcgggtcca gggcgaattt tgcgacaaca tgtcgaggct
     5761 cagcaggacc tgcaggcatg caagcttggc actggccgtc gttttacaac gtcgtgactg
     5821 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     5881 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     5941 cgaatgctag agcagcttga gcttggatca gattgtcgtt tcccgccttc agtttaaact
     6001 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
     6061 taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
     6121 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt
     6181 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
     6241 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
     6301 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
     6361 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
     6421 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
     6481 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
     6541 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
     6601 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
     6661 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
     6721 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
     6781 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
     6841 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
     6901 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
     6961 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
     7021 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
     7081 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
     7141 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
     7201 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
     7261 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
     7321 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
     7381 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
     7441 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
     7501 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
     7561 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
     7621 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
     7681 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
     7741 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
     7801 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
     7861 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
     7921 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
     7981 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
     8041 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
     8101 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
     8161 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
     8221 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
     8281 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
     8341 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgagcggt
     8401 cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag
     8461 ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa
     8521 tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt
     8581 gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg
     8641 ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg
     8701 tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta
     8761 gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg
     8821 gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc
     8881 ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc
     8941 ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc
     9001 atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc
     9061 ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc
     9121 gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg
     9181 gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca
     9241 cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt
     9301 ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat
     9361 gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg
     9421 cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg
     9481 ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc
     9541 acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca
     9601 aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc
     9661 gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt
     9721 gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg
     9781 ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa
     9841 atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc
     9901 gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa
     9961 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
    10021 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
    10081 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt
    10141 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
    10201 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    10261 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    10321 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    10381 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    10441 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    10501 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    10561 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    10621 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    10681 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    10741 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    10801 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
    10861 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    10921 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    10981 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    11041 taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa aatataatat
    11101 tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt
    11161 tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc
    11221 gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg
    11281 ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt
    11341 aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa
    11401 tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag
    11461 ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca
    11521 tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg
    11581 ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc
    11641 tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg ttccacatca
    11701 taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc
    11761 accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt
    11821 tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct
    11881 cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt
    11941 cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt
    12001 caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg
    12061 cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt
    12121 caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg
    12181 ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag
    12241 tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctgg
//
