LOCUS       Exported               11824 bp ds-DNA     circular SYN 01-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11824)
  AUTHORS   Sebastian Schuck
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Sep 29, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11824
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        187..706
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 upstream sequence
     terminator      complement(769..966)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(972..1781)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     promoter        complement(1782..2125)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     misc_feature    2204..2657
                     /label=GAL promoter
                     /note="GAL promoter"
     CDS             2690..4417
                     /codon_start=1
                     /label=OsTIR1(F74G)
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHGADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     terminator      4424..4671
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     misc_feature    4699..8390
                     /label=Padh-GEM-ADHterm
     misc_feature    8391..8829
                     /label=HIS3 downstream sequence
     rep_origin      complement(8889..9344)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9512..9530
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(9570..9588)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(9657..9687)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(10011..10599)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10770..11630)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11631..11735)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
       61 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      121 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
      181 taattccgtt ttaagagctt ggtgagcgct aggagtcact gccaggtatc gtttgaacac
      241 ggcattagtc agggaagtca taacacagtc ctttcccgca attttctttt tctattactc
      301 ttggcctcct ctagtacact ctatattttt ttatgcctcg gtaatgattt tcattttttt
      361 ttttccacct agcggatgac tctttttttt tcttagcgat tggcattatc acataatgaa
      421 ttatacatta tataaagtaa tgtgatttct tcgaagaata tactaaaaaa tgagcaggca
      481 agataaacga aggcaaagat gacagagcag aaagccctag taaagcgtat tacaaatgaa
      541 accaagattc agattgcgat ctctttaaag ggtggtcccc tagcgataga gcactcgatc
      601 ttcccagaaa aagaggcaga agcagtagca gaacaggcca cacaatcgca agtgattaac
      661 gtccacacag gtatagggtt tctggaccat atgatacatg ctctggatca tcgatgaatt
      721 cgagctcgtt ttcgacactg gatggcggcg ttagtatcga atcgacagca gtatagcgac
      781 cagcattcac atacgattga cgcatgatat tactttctgc gcacttaact tcgcatctgg
      841 gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta acatttgatt aaaatagaac
      901 aactacaata taaaaaaact atacaaatga caagttcttg aaaacaagaa tctttttatt
      961 gtcagtactg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     1021 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     1081 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     1141 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     1201 tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt ccagacttgt
     1261 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     1321 attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa
     1381 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     1441 gaatcaggat attcttctaa tacctggaat gctgttttgc cggggatcgc agtggtgagt
     1501 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     1561 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     1621 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     1681 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     1741 tttaatcgcg gcctcgaaac gtgagtcttt tccttaccca tggttgttta tgttcggatg
     1801 tgatgtgaga actgtatcct agcaagattt taaaaggaag tatatgaaag aagaacctca
     1861 gtggcaaatc ctaacctttt atatttctct acaggggcgc ggcgtgggga caattcaacg
     1921 cgtctgtgag gggagcgttt ccctgctcgc aggtctgcag cgaggagccg taatttttgc
     1981 ttcgcgccgt gcggccatca aaatgtatgg atgcaaatga ttatacatgg ggatgtatgg
     2041 gctaaatgta cgggcgacag tcacatcatg cccctgagct gcgcacgtca agactgtcaa
     2101 ggagggtatt ctgggcctcc atgtcgctgg ccgggtgacc cggcggggac gaggcaagct
     2161 aaacagatct ggcgcgcctt aattaacccg gggatccgag ctctagtacg gattagaagc
     2221 cgccgagcgg gtgacagccc tccgaaggaa gactctcctc cgtgcgtcct cgtcttcacc
     2281 ggtcgcgttc ctgaaacgca gatgtgcctc gcgccgcact gctccgaaca ataaagattc
     2341 tacaatacta gcttttatgg ttatgaagag gaaaaattgg cagtaacctg gccccacaaa
     2401 ccttcaaatg aacgaatcaa attaacaacc ataggatgat aatgcgatta gttttttagc
     2461 cttatttctg gggtaattaa tcagcgaagc gatgattttt gatctattaa cagatatata
     2521 aatgcaaaaa ctgcataacc actttaacta atactttcaa cattttcggt ttgtattact
     2581 tcttattcaa atgtaataaa agtatcaaca aaaaattgtt aatatacctc tatactttaa
     2641 cgtcaaggag aaaaaacaga acttagtttc gacggattct agaactagta tgacttattt
     2701 tcctgaagaa gttgtagaac acatattttc ctttttacct gctcaaagag atagaaacac
     2761 cgtctccttg gtctgtaagg tttggtacga aatcgaaaga ttgtctagaa gaggtgtttt
     2821 tgttggtaat tgttacgcag ttagagctgg tagagttgct gcaagattcc caaacgttag
     2881 agcattgact gttaaaggta aacctcatgg tgctgatttc aatttggttc cacctgattg
     2941 gggtggttat gctggtccat ggattgaagc tgcagctaga ggttgtcacg gtttggaaga
     3001 attgagaatg aagagaatgg ttgtttctga tgaatcattg gaattgttag caagatcttt
     3061 tcctagattc agagctttgg ttttgatctc atgtgaaggt ttttccaccg atggtttagc
     3121 agctgttgct tcacattgta agttgttgag agaattggat ttgcaagaaa acgaagttga
     3181 agatagaggt ccaagatggt tatcttgttt tcctgattcc tgtacaagtt tggtttcttt
     3241 gaacttcgca tgtatcaagg gtgaagttaa cgctggtagt ttggaaagat tagtttctag
     3301 atcaccaaat ttgagatctt tgagattgaa cagatccgtt agtgttgata ccttggcaaa
     3361 gatcttgttg agaactccta atttggaaga tttgggtact ggtaacttga cagatgattt
     3421 ccaaacagaa tcatacttca agttgacctc cgctttggaa aagtgtaaga tgttgagatc
     3481 tttgtcaggt ttttgggatg caagtccagt ttgtttgtct tttatatatc ctttgtgtgc
     3541 tcaattgact ggtttgaatt tgtcttacgc accaactttg gatgctagtg atttgacaaa
     3601 gatgatctct agatgtgtta agttgcaaag attgtgggtt ttggattgta tctcagataa
     3661 aggtttgcaa gttgttgctt cttcttgtaa ggatttgcaa gaattgagag tttttccaag
     3721 tgatttctat gttgcaggtt actctgctgt tacagaagaa ggtttagttg cagtttcatt
     3781 gggttgtcct aagttgaact ccttgttgta cttctgtcat caaatgacca acgcagcttt
     3841 ggttactgtt gctaaaaatt gtccaaactt cactagattc agattgtgta tcttggaacc
     3901 aggtaaacct gatgttgtta catcacaacc tttagatgaa ggttttggtg caatcgttag
     3961 agagtgtaag ggtttgcaaa gattgtccat cagtggtttg ttgacagata aggtttttat
     4021 gtacatcggt aaatacgcta agcaattgga aatgttgtca atcgcattcg ctggtgactc
     4081 cgataagggt atgatgcatg ttatgaacgg ttgtaagaac ttaagaaagt tggaaatcag
     4141 agattctcca tttggtgacg cagctttgtt gggtaatttc gcaagatatg aaacaatgag
     4201 atctttgtgg atgtccagtt gtaacgttac cttgaaaggt tgtcaagttt tagcttcaaa
     4261 gatgccaatg ttgaacgttg aagttattaa cgaaagagat ggttccaatg aaatggaaga
     4321 aaaccatggt gacttgccta aagttgaaaa gttatacgtc tacagaacaa ccgcaggtgc
     4381 cagagatgat gctcctaact tcgtcaagat tttatgactc gagtcatgta attagttatg
     4441 tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga aggagttaga
     4501 caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt aagaacgtta
     4561 tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg taacattata
     4621 ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg cggccggtcg
     4681 acctgcagcg tacgcagtgg caaccaaacc catacatcgg gattcctata ataccttcgt
     4741 tggtctccct aacatgtagg tggcggaggg gagatataca atagaacaga taccagacaa
     4801 gacataatgg gctaaacaag actacaccaa ttacactgcc tcattgatgg tggtacataa
     4861 cgaactaata ctgtagccct agacttgata gccatcatca tatcgaagtt tcactaccct
     4921 ttttccattt gccatctatt gaagtaataa taggcgcatg caacttcttt tctttttttt
     4981 tcttttctct ctcccccgtt gttgtctcac catatccgca atgacaaaaa aatgatggaa
     5041 gacactaaag gaaaaattta acgacaaaga cagcaccaac agatgtcgtt gttccagagc
     5101 tgatgagggg tatctcgaag cacacgaaac tttttccttc cttcattcac gcacactact
     5161 ctctaatgag caacggtata cggccttcct tccagttact tgaatttgaa ataaaaaaaa
     5221 gtttgctgtc ttgctatcaa gtataaatag acctgaatcc ctatcagtga tagagatctc
     5281 cctatcagtg atagagattc ttccttgttt ctttttctgc aggtcgacaa acaaatgctc
     5341 gacataaaaa tgaagctact gtcttctatc gaacaagcat gcgatatttg ccgacttaaa
     5401 aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa caactgggag
     5461 tgttgctact ctcccaaaac caaaaggtct ccgctgacta gggcacatct gacagaagtg
     5521 gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg agaagacctt
     5581 ggcatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac aggattattt
     5641 gtacaagata atgtgaataa agatgccgtc acagatagat tggcttcagt ggagactgat
     5701 atgcctctaa cattgagaca gcatagaata agtgcgacat catcatcgga agagagtagt
     5761 aacaaaggtc aaagacagtt gactgtatcg attgactcgg caggatccgg tgacggtgct
     5821 ggtttaatta actctgctgg agacatgaga gctgccaacc tttggccaag cccgctcatg
     5881 atcaaacgct ctaagaagaa cagcctggcc ttgtccctga cggccgacca gatggtcagt
     5941 gccttgttgg atgctgagcc ccccatactc tattccgagt atgatcctac cagacccttc
     6001 agtgaagctt cgatgatggg cttactgacc aacctggcag acagggagct ggttcacatg
     6061 atcaactggg cgaagagggt gccaggcttt gtggatttga ccctccatga tcaggtccac
     6121 cttctagaat gtgcctggct agagatcctg atgattggtc tcgtctggcg ctccatggag
     6181 cacccagtga agctactgtt tgctcctaac ttgctcttgg acaggaacca gggaaaatgt
     6241 gtagagggca tggtggagat cttcgacatg ctgctggcta catcatctcg gttccgcatg
     6301 atgaatctgc agggagagga gtttgtgtgc ctcaaatcta ttattttgct taattctgga
     6361 gtgtacacat ttctgtccag caccctgaag tctctggaag agaaggacca tatccaccga
     6421 gtcctggaca agatcacaga cactttgatc cacctgatgg ccaaggcagg cctgaccctg
     6481 cagcagcagc accagcggct ggcccagctc ctcctcatcc tctcccacat caggcacatg
     6541 agtaacaaag gcatggagca tctgtacagc atgaagtgca agaacgtggt gcccctctat
     6601 gacctgctgc tggagatgct ggacgcccac cgcctacatg cgcccactag ccgtggaggg
     6661 gcatccgtgg aggagacgga ccaaagccac ttggccactg cgggctctac ttcatcgcat
     6721 tccttgcaaa agtattacat cacgggggag gcagagggtt tccctgccac agtcgcggcc
     6781 gcaggtgacg gtgctggttt aattaacatg acggtcgacc atgatttcaa tagcgaagat
     6841 attttattcc ccatagaaag catgagtagt atacaatacg tggagaataa taacccaaat
     6901 aatattaaca acgatgttat cccgtattct ctagatatca aaaacactgt cttagatagt
     6961 gcggatctca atgacattca aaatcaagaa acttcactga atttggggct tcctccacta
     7021 tctttcgact ctccactgcc cgtaacggaa acgataccat ccactaccga taacagcttg
     7081 catttgaaag ctgatagcaa caaaaatcgc gatgcaagaa ctattgaaaa tgatagtgaa
     7141 attaagagta ctaataatgc tagtggctct ggggcaaatc aatacacaac tcttacttca
     7201 ccttatccta tgaacgacat tttgtacaac atgaacaatc cgttacaatc accgtcacct
     7261 tcatcggtac ctcaaaatcc gactataaat cctcccataa atacagcaag taacgaaact
     7321 aatttatcgc ctcaaacttc aaatggtaat gaaactctta tatctcctcg agcccaacaa
     7381 catacgtcca ttaaagataa tcgtctgtcc ttacctaatg gtgctaattc gaatcttttc
     7441 attgacacta acccaaacaa tttgaacgaa aaactaagaa atcaattgaa ctcagataca
     7501 aattcatatt ctaactccat ttctaattca aactccaatt ctacgggtaa tttaaattcc
     7561 agttatttta attcactgaa catagactcc atgctagatg attacgtttc tagtgatctc
     7621 ttattgaatg atgatgatga tgacactaat ttatcacgcc gaagatttag cgacgttata
     7681 acaaaccaag agctctaagc aaatagctaa attatatacg aattaatatt atgattaagt
     7741 gtttacgtga gtgcgatatt tttattacta tcttatacag ttgtatatac tctataaaat
     7801 gagttgtcta ttaattaacg cgatgaatgc tttctgggtt tacctctcca acaactctag
     7861 tttacttctc aatacattca attgtatttg atttgtcaat acttcatcat taatcaattc
     7921 tatagttttg tttttctcgt ttatttccaa atttaatgca tcaattttat tattcaattt
     7981 gtcgttgatt ttggttaatg attttatggt ttgatctctg gcattgattg tttgtgttag
     8041 tttttcatta ttgataatta aattatttaa gttagttatc aactcggtgt tttcaagttt
     8101 caagttttca atttctttag agtttattag atttgtcaaa gtttctgaat tgcttgattg
     8161 gtcctgtaga agagtatttg ttgttgtgga taattgattc aatttttgag acaattgctg
     8221 gaaggcgttg aaatatctag catcaatctc atggtttttt tcccgagagt ctcgtagatt
     8281 caattgtttt aatatatctt gggaccactc ttgatttgaa ctcatggaaa ttaaactggg
     8341 tgttgtgttg tggtgtaatg attgtacccc ctttgcttat aattgtgtgg tgtcgaactt
     8401 ggtttgcaaa gggagaaagt aggagatctc tcttgcgaga tgatcccgca ttttcttgaa
     8461 agctttgcag aggctagcag aattaccctc cacgttgatt gtctgcgagg caagaatgat
     8521 catcaccgta gtgagagtgc gttcaaggct cttgcggttg ccataagaga agccacctcg
     8581 cccaatggta ccaacgatgt tccctccacc aaaggtgttc ttatgtagtg acaccgatta
     8641 tttaaagctg cagcatacga tatatataca tgtgtatata tgtataccta tgaatgtcag
     8701 taagtatgta tacgaacagt atgatactga agatgacaag gtaatgcatc attctatacg
     8761 tgtcattctg aacgaggcgc gctttccttt tttctttttg ctttttcttt ttttttctct
     8821 tgaactcgac ggatcatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     8881 gcatcaggaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     8941 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     9001 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     9061 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     9121 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     9181 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     9241 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     9301 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcgcgcc attcgccatt
     9361 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
     9421 ggcgaagggg ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc
     9481 acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gcgaattgga
     9541 ggggggggcc cggtacccag cttttgttcc ctttagtgag ggttaattcc gagcttggcg
     9601 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9661 ataggagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag gtaactcaca
     9721 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9781 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9841 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9901 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9961 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    10021 ctcggccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10081 acaggactat aaagatacca ggcgttcccc cctggaagct ccctcgtgcg ctctcctgtt
    10141 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10201 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10261 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10321 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10381 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10441 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10501 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10561 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10621 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10681 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10741 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10801 tcagcgatct gtctatttcg ttcatccata gttgcctgac tgcccgtcgt gtagataact
    10861 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10921 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10981 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    11041 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    11101 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    11161 acatgatccc ccatgttgtg aaaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    11221 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    11281 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11341 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11401 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    11461 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11521 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11581 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11641 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11701 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11761 gacgtcttat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
    11821 tcgc
//
