LOCUS       pDQM138_SP6_2xLy        3395 bp    DNA        linear       14-OCT-2025
DEFINITION                               SP6-2xLynk-mGold2t in pTwist Amp High
            Copy
FEATURES                                 Location/Qualifiers
COMMENT                                  This file was created using tools
            provided by Twist Bioscience
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..18
                     /locus_tag="SP6"
                     /label="SP6"
                     /ApEinfo_label="SP6"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    19..24
                     /locus_tag="SpeI"
                     /label="SpeI"
                     /ApEinfo_label="SpeI"
                     /ApEinfo_fwdcolor="#ff0014"
                     /ApEinfo_revcolor="#ff0014"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     regulatory      25..30
                     /note="note: vertebrate consensus sequence
                     forstronginitiation of translation (Kozak,
                     1987)regulatory_class:other: "
                     /locus_tag="Kozak sequence"
                     /label="Kozak sequence"
                     /ApEinfo_label="Kozak sequence"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    31..156
                     /locus_tag="2xLynk_0.99"
                     /label="2xLynk_0.99"
                     /ApEinfo_label="2xLynk_0.99"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    880..882
                     /locus_tag="mStayGold(J)-zf99"
                     /label="mStayGold(J)-zf99"
                     /ApEinfo_label="mStayGold(J)-zf99"
                     /ApEinfo_fwdcolor="#00ff0b"
                     /ApEinfo_revcolor="#00ff0b"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    883..907
                     /locus_tag="hulc-psw-3' linker"
                     /label="hulc-psw-3' linker"
                     /ApEinfo_label="hulc-psw-3' linker"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    941..1174
                     /locus_tag="SV40"
                     /label="SV40"
                     /ApEinfo_label="SV40"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             163..879
                     /translation=IUPAC
                     /locus_tag="mGold2s"
                     /label="mGold2s"
                     /ApEinfo_label="mGold2s"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    435..435
                     /locus_tag="New Feature"
                     /label="New Feature"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    651..651
                     /locus_tag="New Feature(1)"
                     /label="New Feature(1)"
                     /ApEinfo_label="New Feature"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    778..780
                     /locus_tag="T"
                     /label="T"
                     /ApEinfo_label="T"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
     misc_feature    1419..2007
                     /locus_tag="colE"
                     /label="colE"
                     /ApEinfo_label="colE"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="#00ffff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 ATTTAGGTGA CACTATAGaC TAGTGCCACC ATGGGCTGCA TCAAGAGCAA GCGTAAGGAC
       61 AACCTGAACG ATGATGAAGC TGCTATGGGA TGTATCAAAT CTAAGCGTAA GGATAACCTT
      121 AATGATGATG AGGGAGCAGC TGCTCCCGCT GCTGCTggat ccATGGCGAG CAAGGGCGAA
      181 GAGCTGTTCA CCGGAGTcGT cCCCATCCTG GTCGAACTGG ATGGAGACAT CAACGGTCAC
      241 AAGTTCAGCG TCTCCGGCGA GGGCGAGGGT GATGCTACCT ACGGCAAGCT GACCCTGAAG
      301 TTCATCTGCA CCACCGGCAA GCTGCCTGTC CCCTGGCCCA CCCTCGTGAC CAGCCTGGGC
      361 TACGGCCTGC AGTGCTTCGC TCGCTACCCT GATAACATGA AGCGTCACGA TTTCTTCAAG
      421 TCCGCTATGC CCGAaGGaTA CGTCCAGGAG CGTACCATCT TCTTCGAgGA TGACGGCAAC
      481 TACAAGACCC GCGCTGAaGT cAAGTTTGAA GGCGGAACCC TGGTGAATCG CGTCGAACTG
      541 AAGGGAATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA ATACAACTAC
      601 AACTGCCACA ACGTCTTTAT CACCGCCGAt AGaCAgCAaA ACGGCATCAA aGTCAACTTC
      661 AAGATCCGTC ACAACATCGA AGTCGGAGGC GTGCAGCTCG CCGACCACTA CCAGCAGAAC
      721 ACCCCCATTG GCGATGGACC CGTCCTGCTG CCCGACAACC ACTACCTGAG CTACCAaaCC
      781 AAGCTGTCCA AAGATCCCAA TGAGAAACGC GATCACATGG TCCTGCTGGA GTTCGTGACC
      841 GCCGCCGGAA TCACTCTCTC aATGGATGAG CTGTACAAGT AAgaattcat acgtatccgg
      901 aaccggtGCT AGCTTGACTG ACTGAGATAC AGCGTACCTT CAGCTCACAG ACATGATAAG
      961 ATACATTGAT GAGTTTGGAC AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG
     1021 TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA
     1081 CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA
     1141 AAGCAAGTAA AACCTCTACA AATGTGGTAT TGGCAGGCTA GGTGGAGGCT CAGTGATGAT
     1201 AAGTCTGCGA TGGTGGATGC ATGTGTCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA
     1261 TCCGCTCAGA GGGCACAATC CTATTCCGCG CTATCCGACA ATCTCCAAGA CATTAGGTGG
     1321 AGTTCAGTTC GGCGTATGGC ATATGTCGCT GGAAAGAACA TGTGAGCAAA AGGCCAGCAA
     1381 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT
     1441 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA
     1501 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG
     1561 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA
     1621 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA
     1681 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG
     1741 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG
     1801 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA
     1861 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC
     1921 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG
     1981 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC
     2041 GCTCTATTCA ACAAAGCCGC CGTCCCGTCA AGTCAGCGTA AATGGGTAGG GGGCTTCAAA
     2101 TCGTCCTCGT GATACCAATT CGGAGCCTGC TTTTTTGTAC AAACTTGTTG ATAATGGCAA
     2161 TTCAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG
     2221 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
     2281 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC
     2341 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG AGCCACGCTC
     2401 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG
     2461 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG
     2521 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC
     2581 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC
     2641 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG
     2701 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC
     2761 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG
     2821 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC
     2881 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT
     2941 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG
     3001 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA
     3061 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT
     3121 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG
     3181 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCAGATAC
     3241 CTGAAACAAA ACCCATCGTA CGGCCAAGGA AGTCTCCAAT AACTGTGATC CACCACAAGC
     3301 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTCATGCAT AATCCGCACG
     3361 CATCTGGAAT AAGGAAGTGC CATTCCGCCT GACCT  
//
