LOCUS       pTKIU-Tyr_GFP-SU        7732 bp    DNA     circular SYN 17-AUG-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene 8.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          162..187
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     primer_bind     complement(135..183)
                     /label=pTet-sfGFP_gibR
                     /note="color: black; sequence: 
                     AGTCCGGAACGTCGTACGGGTACATATGGATACCGCCTGAGTCAGTACT"
     CDS             159..188
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #339966"
                     /translation="MYPYDVPDYA"
     primer_bind     165..212
                     /label=pTet-sfGFP_gibF
                     /note="color: black; sequence: 
                     CCGTACGACGTTCCGGACTACGCCCGTAAAGGCGAAGAGCTGTTCACT"
     CDS             189..899
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=sfGFP
                     /note="color: #05fd14"
                     /translation="RKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(874..917)
                     /label=sfGFP-SUMO_gibR
                     /note="color: black; sequence: 
                     cgagtcCATCAATTGGCCTTTGTACAGTTCATCCATGCCATGCG"
     primer_bind     891..929
                     /label=sfGFP-SUMO_gibF
                     /note="color: black; sequence: 
                     CTGTACAAAGGCCAATTGATGgactcggaggtcaaccag"
     CDS             909..1199
                     /codon_start=1
                     /locus_tag="SUMO_myc_opt"
                     /label=SUMO
                     /label=SUMO_myc_opt
                     /note="color: #ff9900"
                     /translation="MDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLR
                     RLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAYREQIGG"
     primer_bind     complement(1183..1227)
                     /label=SUMO-Tyr_mCherry_gibR
                     /note="color: black; sequence: 
                     TgGCTGTGCCAGATTGGACGCGCAGGTAgccaccgatctgctcgc"
     CDS             1200..1228
                     /codon_start=1
                     /locus_tag="Nend tag"
                     /label=Nend tag
                     /note="color: #c0c0c0"
                     /translation="YLRVQSGTA"
     primer_bind     1208..1260
                     /label=SUMO-Tyr_mCherry_gibF
                     /note="color: black; sequence: 
                     CGTCCAATCTGGCACAGCcAGCCTGCAGGtctcgaagggggaagaggacaaca"
     CDS             1237..1940
                     /codon_start=3
                     /label=mCherry
                     /note="color: #ff0000"
                     /translation="SKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ
                     TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED
                     GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE
                     IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG
                     RHSTGGMDELYK"
     primer_bind     complement(1936..2003)
                     /label=mCherry-Ulp1_gibR
                     /note="color: black; sequence: 
                     aaaTCCTTCCTCCTTTCTTAAGGCGGCCGCTTACAGATCCTCTTCTGAGATGAGTTTTT
                     GTTCtttgt"
     CDS             1941..1970
                     /codon_start=1
                     /locus_tag="Myc tag"
                     /label=Myc tag
                     /note="color: #0080ff"
                     /translation="EQKLISEEDL"
     primer_bind     complement(1970..1984)
                     /label=Ulp1-pTet_gibR
                     /note="color: black; sequence: 
                     AAGGCGGCCGCTTActtatcgtcgtcgtctttgtagtcgat"
     primer_bind     1978..2024
                     /label=mCherry-Ulp1_gibF
                     /note="color: black; sequence: 
                     CCGCCTTAAGAAAGGAGGAAGGAtttttcATGCACGCAACCCCTGAG"
     misc_feature    1986..2000
                     /label=RBS
                     /note="color: #00ffff"
     CDS             2007..2798
                     /codon_start=1
                     /locus_tag="Rv0005p_CthUlp1_syn"
                     /label=CthUlp1
                     /label=Rv0005p_CthUlp1_syn
                     /note="color: #8080ff"
                     /translation="MHATPEESIRIMSGLRPPARQLITPLSEEWRSRVEAARNANPATE
                     LAKTLEGQPLVRRDFEEKLLPATAWLNDNVIIGAIFYIADYVNTKKGAPNQEPKCTAFT
                     SFFWPRLLSHGPGGCGRLLRRANVRKANFLDIDTILIPICESSHWTLAVIRPGRRTVSH
                     LDSMAAGRGSERVKAKLLELVKFVLEDQFVEAEWQAVDFQAPRQTNGWDCGVFTITNAI
                     CLALGVDPAQAYTEAQLPLQRQRIAAVLLNGGFKGDFTLDDL"
     CDS             2799..2867
                     /codon_start=1
                     /label=3x-FLAG
                     /note="color: #0000ff"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(2838..2878)
                     /label=Ulp1-pTet_gibR
                     /note="color: black; sequence: 
                     AAGGCGGCCGCTTActtatcgtcgtcgtctttgtagtcgat"
     primer_bind     2858..2890
                     /label=Ulp1-pTet_gibF
                     /note="color: black; sequence: 
                     cgataagTAAGCGGCCGCCTTAAAAAAAAAAGC"
     rep_origin      complement(3020..3608)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(3160..3179)
                     /label=pLJR962_seqR
                     /note="color: black; sequence: GGACAGGTATCCGGTAAGCG"
     terminator      3727..3754
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
                     /note="color: #ffffff"
     CDS             4144..4767
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /note="color: #993366"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             5483..6481
                     /codon_start=1
                     /label=mycobacterial integrase
                     /note="color: #33cccc"
                     /translation="MDAEAWLAGEKRLIEMETWTPPQDRAKKAAASAITLEEYTRKWLV
                     ERDLADGTRDLYSGHAERRIYPVLGEVAVTEMTPALVRAWWAGMGRKHPTARRHAYNVL
                     RAVMNTAVEDKLIAENPCRIEQKAADERDVEALTPEELDIVAAEIFVHYRIAAYILAWT
                     SLRFGELIELRRKDIVDDGMTMKLRVRRGASRVGNKIVVGNAKTVRSKRPVTVPPHVAE
                     MIRAHMKDRTKMNKGPEAFLVTTTQGNRLSKSAFTKSLKRGYAKIGRPELRIHDLRAVG
                     ATFAAQAGATTKELMARLGHTTPRMAMKYQMASEARDEAIAEAMSKLAKTS"
     CDS             6781..7596
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /note="color: #ccffcc"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7634..7653
                     /label=pLJR962_seqF
                     /note="color: black; sequence: TTACGCTGACTTGACGGGAC"
ORIGIN
        1 cgtggcaaag caaaagttca aaatcaccaa ctggtccacc tacaacaaag ctctcaccaa
       61 ccgtggtctg accagggaaa atagccctct gacctgggat ttgcgatccc tatcagtgat
      121 agatataatc tgggagtact gactcaggcg gtatccatat gtacccgtac gacgttccgg
      181 actacgcccg taaaggcgaa gagctgttca ctggtgtcgt ccctattctg gtggaactgg
      241 atggtgatgt caacggtcat aagttttccg tgcgtggcga gggtgaaggt gacgcaacta
      301 atggtaaact gacgctgaag ttcatctgta ctactggtaa actgccggta ccttggccga
      361 ctctggtaac gacgctgact tatggtgttc agtgctttgc tcgttatccg gaccatatga
      421 agcagcatga cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt
      481 cctttaagga tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc
      541 tggtaaaccg cattgagctg aaaggcattg actttaaaga agacggcaat atcctgggcc
      601 ataagctgga atacaatttt aacagccaca atgtttacat caccgccgat aaacaaaaaa
      661 atggcattaa agcgaatttt aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg
      721 ctgatcacta ccagcaaaac actccaatcg gtgatggtcc tgttctgctg ccagacaatc
      781 actatctgag cacgcaaagc gttctgtcta aagatccgaa cgagaaacgc gatcatatgg
      841 ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg catggatgaa ctgtacaaag
      901 gccaattgat ggactcggag gtcaaccagg aggccaagcc cgaggtcaaa ccggaagtga
      961 agccggagac ccacatcaac ctgaaagtga gcgacggtag ctcggagatc ttcttcaaga
     1021 tcaagaagac caccccgctg cgccggctga tggaagcctt cgcgaagcgc caaggcaaag
     1081 agatggactc gctccgcttc ctctacgacg gtatccgcat ccaggccgac caaacccccg
     1141 aggatctgga catggaggac aatgacatca tcgaagctta ccgcgagcag atcggtggct
     1201 acctgcgcgt ccaatctggc acagccagcc tgcaggtctc gaagggggaa gaggacaaca
     1261 tggccatcat caaggaattc atgcggttca aggtgcacat ggagggcagc gtcaatgggc
     1321 atgagttcga gatcgagggg gaaggtgagg ggcggccgta cgaaggcacc cagacggcca
     1381 agctcaaggt cacgaagggc ggcccgctcc cgttcgcctg ggacatcctg agcccgcagt
     1441 tcatgtacgg ctcgaaggcc tacgtgaagc atccggccga catcccggac tacctcaagc
     1501 tctcgttccc cgaaggcttc aagtgggagc gtgtcatgaa cttcgaggac ggcggtgtgg
     1561 tcacggtcac ccaggactcc tcgctgcaag atggggaatt catctacaag gtcaagctcc
     1621 gggggaccaa cttcccctcg gacggtccgg tgatgcagaa gaagaccatg ggttgggaag
     1681 ccagctccga gcgcatgtac ccggaggacg gtgccctgaa gggggaaatc aagcagcggc
     1741 tcaagctgaa ggacggtggt cattacgacg ccgaggtgaa gaccacctac aaggccaaaa
     1801 agccggtcca actgcccggc gcctacaacg tgaacatcaa actggatatc acctcgcaca
     1861 acgaggacta caccatcgtc gagcagtacg agcgcgccga gggtcgccac agcaccggtg
     1921 gcatggatga actgtacaaa gaacaaaaac tcatctcaga agaggatctg taagcggccg
     1981 ccttaagaaa ggaggaagga tttttcatgc acgcaacccc tgaggagagt attcggatca
     2041 tgtccgggct ccggccgccc gcccggcagc tgatcacccc cctcagcgag gaatggcggt
     2101 cccgggtgga ggcggcccgg aatgccaacc cggcgacgga actcgcgaag acgctggagg
     2161 gccagccgct ggtgcgccgg gacttcgaag aaaagctcct gcccgccacc gcctggctga
     2221 acgacaacgt catcatcggg gccatcttct acatcgcgga ctacgtgaac accaagaaag
     2281 gggcgccgaa ccaggagccc aagtgcaccg ccttcacgtc gttcttctgg ccccggctgc
     2341 tctcccacgg gccgggcggc tgcgggcgcc tcctgcgccg cgcgaacgtg cggaaggcca
     2401 acttcctgga tatcgacacc atcctgatcc cgatctgcga aagctcgcac tggaccctgg
     2461 cggtgatccg gcccgggcgg cgcaccgtct cgcacctcga ctcgatggcc gcggggcgcg
     2521 gctccgagcg ggtgaaggcg aagctcctcg aactggtcaa gttcgtgctg gaagaccagt
     2581 tcgtggaggc cgaatggcaa gcggtggact tccaggcgcc ccgccagacg aacggctggg
     2641 actgcggagt cttcaccatc accaacgcga tctgtctggc gctgggcgtg gacccggccc
     2701 aggcgtacac cgaagcccag ctgcccctac agcgccagcg catcgcggcg gtcctgctca
     2761 acggtggctt caagggtgac ttcaccctcg acgacctgga ctacaaggac cacgatggtg
     2821 attataagga ccacgacatc gactacaaag acgacgacga taagtaagcg gccgccttaa
     2881 aaaaaaaagc cccgcgattg cggggctttt tttttttaat acggttatcc acagaatcag
     2941 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     3001 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     3061 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     3121 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     3181 cctttctccc ctcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     3241 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     3301 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     3361 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3421 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     3481 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3541 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3601 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcatgga acaataaacg
     3661 aaaggctcag tcgaaagact gggcctttcg tttatctgtt gtttgaaagc cgctgattag
     3721 ctaagcagaa ggccatcctg acggatggcc tttttctaga aaaaaaaaaa gcgccgcaac
     3781 tgcggcgctt tttttttttt aattaaagaa atattggatc gtcggcaccg tcacggccgt
     3841 gggaggcggc acgatccgcg acgtgatgat cggccgcatc cccacggtgc tgcgcagtga
     3901 gctctacgcc atcccggcgt tgatctgtgc gttcgcacgc acaggcccgg tgtgagaagg
     3961 gtctctgacg agcgggagaa cccacccggg gtgggcgagt ttgtcctgcg tgtgctcggt
     4021 cgagtaggct ctgggagtac ccgtgtgtac gaccagcacg gcatacatca tttcgacgcc
     4081 gagagattcg ccgcccgaaa tgagcacgat ccgcatggta ccatcaggag gaatcacttc
     4141 gcaatgtcta gattagataa aagtaaagtg attaacagcg cattagagct gcttaatgag
     4201 gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga agcttggtgt agagcagcct
     4261 acattgtatt ggcatgtaaa aaataagcgg gccctgctcg acgccttagc cattgagatg
     4321 ttagataggc accatactca cttttgccct ttagaagggg aaagctggca agatttttta
     4381 cgtaataacg ctaaaagttt tagatgtgct ttactaagtc atcgcgatgg agcaaaagta
     4441 catttaggta cacggcctac agaaaaacag tatgaaactc tcgaaaatca attagccttt
     4501 ttatgccaac aaggtttttc actagagaac gcgttatatg cactcagcgc tgtggggcat
     4561 tttaccttag gttgcgtatt ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa
     4621 acacctacta ctgatagtat gccgccatta ttacgacaag ctatcgaatt atttgatcac
     4681 caaggtgcag agccagcctt cttattcggc cttgaattga tcatctgcgg attagaaaaa
     4741 caacttaaat gtgaaagtgg gtcttaatga ccatgggcta gcgctttagc taattaattt
     4801 aaaataaaaa aggggacctc tagggtcccc aattaaatag ttaacactag tattaaaaaa
     4861 aaaagccccg cgattgcggg gctttttttt ttcgacggcg agggttccga ccgctgcaac
     4921 tcccggtgca accttgtccc ggtctattct cttcactgca ccagctccaa tctggtgtga
     4981 atgcccctcg tctgttcgcg caggcggggg gctctattcg tttgtcagca tcgaaagtag
     5041 ccagatcagg gatgcgttgc aaccgcgtat gcccaggtca gaagagtcgc acaagagttg
     5101 cagacccctg gaaagaaaaa tggccagagg gcgaaaacac cctctgacca gcggagcggg
     5161 cgacgggaat cgaacccgcg tagctagttt ggaagaatgg gtgtctgccg accacatata
     5221 tgggccggtc aagataggtt tttaccccct ctcggctgca tcctctaagt ggaaagaaat
     5281 tgcaggtcgt agaagcgcgt tgaagcctga gagttgcaca ggagttgcaa cccggtagcc
     5341 ttgttcacga cgagaggaga cctagttggc acgtcgcgga tggggatcgc tgaagactca
     5401 gcgcagcggg aggatccaag cctcatacgt caacccgcag gacggtgtga ggtactacgc
     5461 gctgcagacc tacgacaaca agatggacgc cgaagcctgg ctcgcgggcg agaagcggct
     5521 catcgagatg gagacctgga cccctccaca ggaccgggcg aagaaggcag ccgccagcgc
     5581 catcacgctg gaggagtaca cccggaagtg gctcgtggag cgcgacctcg cagacggcac
     5641 cagggatctg tacagcgggc acgcggagcg ccgcatctac ccggtgctag gtgaagtggc
     5701 ggtcacagag atgacgccag ctctggtgcg tgcgtggtgg gccgggatgg gtaggaagca
     5761 cccgactgcc cgccggcatg cctacaacgt cctccgggcg gtgatgaaca cagcggtcga
     5821 ggacaagctg atcgcagaga acccgtgccg gatcgagcag aaggcagccg atgagcgcga
     5881 cgtagaggcg ctgacgcctg aggagctgga catcgtcgcc gctgagatct tcgtgcacta
     5941 ccggatcgcg gcatacatcc tggcgtggac gagcctccgg ttcggagagc tgatcgagct
     6001 tcgccgcaag gacatcgtgg acgacggcat gacgatgaag ctccgggtgc gccgtggcgc
     6061 ttcccgcgtg gggaacaaga tcgtcgttgg caacgccaag accgtccggt cgaagcgtcc
     6121 tgtgacggtt ccgcctcacg tcgcggagat gatccgagcg cacatgaagg accgtacgaa
     6181 gatgaacaag ggccccgagg cattcctggt gaccacgacg cagggcaacc ggctgtcgaa
     6241 gtccgcgttc accaagtcgc tgaagcgtgg ctacgccaag atcggtcggc cggaactccg
     6301 catccacgac ctccgcgctg tcggcgctac gttcgccgct caggcaggtg cgacgaccaa
     6361 ggagctgatg gcccgcctcg gtcacacgac tcctaggatg gcgatgaagt accagatggc
     6421 gtctgaggcc cgcgacgagg ctatcgctga ggcgatgtcc aagctggcca agacctcctg
     6481 aaacgcaaaa agcccccctc ccaaggacac tgagtcctaa agaggggggt ttcttgtcag
     6541 tacgcgaaga accacgcctg gccgcgagcg ccagcaccgc cgctctgtgc ggagacctgg
     6601 gcaccagccc cgccgccgcc aggagcattg ccgttcccgc cagctagcca cctgacgtcg
     6661 gggggggggg aaagccacgt tgtgtctcaa aatctctgat gttacattgc acaagataaa
     6721 aatatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
     6781 atgagccata ttcaacggga aacgtcttgc tcgaggccgc gattaaattc caacatggat
     6841 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     6901 tatcgcttgt atgggaagcc ccatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     6961 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     7021 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     7081 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     7141 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     7201 tttaacagcg atcgcgtatt tcgcctcgct caggcgcaat cacgaatgaa taacggtttg
     7261 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     7321 gaaatgcata atcttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     7381 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     7441 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     7501 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     7561 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     7621 cactggcaga gcattacgct gacttgacgg gacggcggct ttgttgaata aatcgaactt
     7681 ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt tc
//
