LOCUS       pBK-glnS-EcTyrRS-VSMA   3249 bp ds-DNA     circular     01-OCT-2025
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name:
            003_pBK-glnS-EcYRS-VSMA_contig.gbk Annotated with pLannotate v1.2.1
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(858..2129)
                     /label="tyrS"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="swissprot"
                     /identity="98.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALVCGFDPTADSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVDKIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQRLNREGQGISFTEFSYNLLQGYSMACANKQYGVVLQIGGSDQWGNITSGIDLTRRLHQNQVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFTFMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSALSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQSDPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     CDS             complement(2313..3128)
                     /label="KanR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pLannotate"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt gcgaagcgga
      661 attaattcgc gaagaaaagc agaaaaaacg ccgctgaacg cggcgttttg gattgtttgc
      721 tgtttcttgc cggatgcggc gtgaacgcct tatccggcct acaaaagcac gcaaactcaa
      781 tatattgcag agatcatgta ggcctgataa gcgtagcgca tcaggcaatt tagcgtttga
      841 aactgcagcc atggttattt ccagcaaatc agacagtaat tctttttacc gcgacgcagt
      901 aaggtaaaac gaccaaacag acgatcttct tctttaaaga agtattcagg atcggactgt
      961 ttttcaccgt taatggtgat ggcattggag gcgatagttt tacgtgcctg accacgggaa
     1021 ggttgcagtt cagaatcgac cagtgcctgc atcaggtctg cgcccttttc catctcaacc
     1081 atcggtacgc cgtcctgcgc cagctgttcg aagtccgctt cactcagcgc actcaaagaa
     1141 ccgctgaaca ggcattcggt aatacgtttt gccgcctgta aaccttcttc accgtgaacc
     1201 agacgagtca cctgctccgc cagtacatac tgggcgcgcg gtgctttacc gctgttttta
     1261 tcttcttctt ccagggcgtt gatctcttca atgctcataa aggtgaagaa cttcaggaag
     1321 cggtaaacgt cggcatccgc agtgttgatc cagaactggt agaatttgta cgggctggtt
     1381 ttcttcggat ctaaccagac tgcgccgcct tcagttttac caaatttggt gccatctgct
     1441 ttagtgatca gcggaacggt caggccaaac acctgattct gatgcagacg acgggtcagg
     1501 tcgataccag aagtgatgtt accccactgg tcagaaccac caatttgcag caccacaccg
     1561 tactgtttgt tcgcacaggc catactataa ccctgcaaca ggttgtagga aaactcagtg
     1621 aacgaaatcc cctgaccttc acggttgaga cgctgcttaa ccgcttcttt gttgatcatc
     1681 tggttaacgg agaagtgttt gccaatatcg cgcaggaagg tcagcacatt catattgccg
     1741 aaccagtcat agttgttcgc cgcgatagca gagttttctc cacagtcgaa atcgaggaac
     1801 ggggcaacct gcttacggat tttgtccacc cactcctgaa cagtttcttc ggtgttcagc
     1861 ttacgctcgg cagctttgaa gctcgggtcg ccaatcagac ccgtcgcgcc gcctaccagc
     1921 gcaaccggct tgtggcccgc ctgctggaag cgtttcaggc ataacaatgg aacaagatgc
     1981 cccaaatgca agctgtcagc ggtaggatcg aagccgcaca cgagcgcgat cgggccttgc
     2041 gccagtcgct ctgctaacgc ttcctcgtcc gtcacctggg ctaccagccc ccgctcttgc
     2101 aattgtttaa tcaagttact gcttgccata tgggattcct caaagcgtaa acaacgtata
     2161 acggcgtatg atcttataag cgggacaggc tgacaacccg agcggccgcg gatccttcaa
     2221 ctcagcaaaa gttcgattta ttcaacaaag ccgccgtccc gtcaagtcag cgtaatgctc
     2281 tgccagtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag catcaaatga
     2341 aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt
     2401 aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct
     2461 gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg
     2521 ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagctta
     2581 tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc
     2641 gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg
     2701 ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc
     2761 gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc
     2821 ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg
     2881 gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca
     2941 ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac
     3001 aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat
     3061 aaatcagcat ccatgttgga atttaatcgc ggcctcgagc aagacgtttc ccgttgaata
     3121 tggctcataa caccccttgt attactgttt atgtaagcag acagttttat tgttcatgac
     3181 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     3241 aggatcttc
//