LOCUS       hSyn-PreSynBar-h        7247 bp DNA     circular SYN 01-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7247)
  AUTHORS   Stanford University
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7247
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /label=ITR
                     /label=ITR(1)
                     /note="color: #00ffff"
     misc_feature    151..628
                     /label=synapsin promoter
                     /note="color: #4eff51"
     misc_feature    642..646
                     /label=Kozak consensus
                     /note="color: #ffd92b"
     CDS             648..692
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /note="color: #993366"
                     /translation="MSALLILALVGAAVA"
     misc_feature    693..734
                     /label=V5 tag
                     /note="color: #ff80ff"
     CDS             747..2516
                     /codon_start=1
                     /label=Nrxn1b-splitGFP-ORF
                     /note="color: #993366"
                     /translation="ASSLGAHHIHHFHGSSKHHSVPIAIYRSPASLRGGHAGTTYIFSK
                     GGGQITYKWPPNDRPSTRADRLAIGFSTVQKEAVLVRVDSSSGLGDYLELHIHQGKIGV
                     KFNVGTDDIAIEESNAIINDGKYHVVRFTRSGGNATLQVDSWPVIERYPASGGGSGGGS
                     GGGMGGTSMSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLKFICTTG
                     KLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGKYKTRA
                     VVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKANFTVRH
                     NVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKGGGSGGGSGGSGGR
                     QLTIFNSQATIIIGGKEQGQPFQGQLSGLYYNGLKVLNMAAENDANIAIVGNVRLVGEV
                     PSSMTTESTATAMQSEMSTSIMETTTTLATSTARRGKPPTKEPISQTTDDILVASAECP
                     SDDEDIDPCEPSSGGLANPTRVGGREPYPGSAEVIRESSSTTGMVVGIVAAAALCILIL
                     LYAMYKYRNRDEGS"
     misc_feature    1209..1937
                     /label=GFP/1-10/+/L
                     /note="color: #a6acb3"
     CDS             2337..2346
                     /codon_start=1
                     /label=Nrxn1b-splitGFP-ORF
                     /note="color: #993366"
                     /translation="GGL"
     misc_feature    2517..2585
                     /label=linker1
                     /note="color: #a6acb3"
     regulatory      2580..2589
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     misc_feature    2586..2651
                     /label=lambda peptide
                     /note="color: #a6acb3"
     misc_feature    2652..2732
                     /label=linker2
                     /note="color: #a6acb3"
     CDS             2733..2864
                     /codon_start=1
                     /label=Nrxn1b-splitGFP-ORF
                     /note="color: #993366"
                     /translation="VDESRNYISNSAQSNGAVVKEKQPSSAKSANKNKKNKDKEYYV"
     misc_feature    2865
                     /label=EcoRI
                     /note="color: #46ff6e"
     misc_feature    2874..3482
                     /label=WPRE
                     /note="color: #a0ff99"
     misc_feature    3496..3720
                     /label=PolyA
                     /note="color: #ff2549"
     misc_feature    3728..4205
                     /label=synapsin promoter
                     /note="color: #4eff51"
     misc_feature    4159..4205
                     /label=hSyn exon1
                     /note="color: #a6acb3"
     misc_feature    4219..4329
                     /label=2BoxB
                     /note="color: #a6acb3"
     misc_feature    4219..4329
                     /label=barII
                     /note="color: #a6acb3"
     protein_bind    4266..4284
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
                     /note="color: #31849b"
     protein_bind    4290..4308
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
                     /note="color: #31849b"
     misc_feature    4336..4343
                     /label=barcode region
                     /note="color: #a6acb3"
     polyA_signal    4350..4471
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #ff0000"
     misc_feature    4472..4482
                     /label=PolyA
                     /note="color: #ff2549"
     misc_feature    4511..4651
                     /label=ITR
                     /note="color: #00ffff"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc cgccaccatg tctgcacttc
      661 tgatcctagc tcttgttgga gctgcagttg ctggcaagcc gatccctaac cctctgctgg
      721 gcctggacag cactcttgcg gccgcggcat ccagtttggg agcgcaccac atccaccatt
      781 tccatggcag cagcaagcat cattcagtgc ctattgcaat ctacaggtca ccagcatcct
      841 tgcgaggcgg acacgctggg acgacgtata tctttagcaa aggtggtgga caaattacat
      901 ataagtggcc tcctaatgac cgacccagta cacgagcaga caggctggcc atcgggttta
      961 gcactgttca gaaggaagca gtgttggtgc gtgtggacag ttcctcgggc ctgggtgact
     1021 accttgagct gcacatacac caaggaaaaa ttggagttaa atttaatgtt gggacagatg
     1081 acatcgccat tgaggagtcc aatgcaatca ttaatgatgg aaaataccat gtagtgcgtt
     1141 tcacgaggag tggtggcaat gccacgttac aggtggacag ctggccagtc atcgaacgat
     1201 accctgcatc cggaggaggt tcaggtggag gttcaggagg aggaatgggt ggaacaagca
     1261 tgagcaaagg tgaggaactg ttcaccggcg tcgtacctat tcttgtggag ctggacgggg
     1321 acgtcaatgg acacaaattc tcagttcgcg gcgagggcga gggggatgct accatcggta
     1381 agctgaccct caaattcatc tgcactacgg gcaagctgcc ggttccctgg cctactctgg
     1441 tcactacact gacttacggg gtccaatgct tctccagata tcccgaccac atgaagcggc
     1501 acgacttctt taaaagcgcc atgccggagg ggtacgtgca ggaacgaacc atttccttca
     1561 aagatgatgg taagtataag accagagctg tcgtcaagtt tgagggcgac accctcgtga
     1621 accgcatcga acttaaggga accgatttta aggaagacgg taacatcctg ggccacaagc
     1681 tggagtacaa tttcaactca cataatgtgt acattacggc agacaagcag aaaaacggga
     1741 tcaaagccaa ttttacagta cggcacaatg tcgaggacgg cagcgtccag ctggcagatc
     1801 attaccagca aaacacgcct atcggagatg ggccggtgct gcttccagat aaccattacc
     1861 tttccacaca gaccgtgctg agcaaggacc ctaacgagaa gggtggaggt tctggaggtg
     1921 gttctggagg ttccggaggg cgtcagctca caatcttcaa tagccaagca accataataa
     1981 ttggcgggaa agagcagggc cagcccttcc agggccagct ctctgggctt tactacaatg
     2041 gcttgaaagt tctgaatatg gcggcagaga acgatgccaa catcgccata gtgggaaatg
     2101 tgaggctggt cggtgaagtg ccttcctcta tgacaactga gtcgacagcc actgccatgc
     2161 agtccgagat gtccacctca atcatggaga ccaccacaac cctggctacc agcacagctc
     2221 gacgaggaaa gccccccaca aaggagccta tcagccagac cacggatgat atccttgtgg
     2281 cctcggcaga gtgtcccagc gacgatgagg acattgaccc ctgtgagccg agctcaggtg
     2341 ggttagccaa ccccacccga gtgggcggac gcgaaccata cccaggctcg gcagaggtga
     2401 tccgggagtc tagcagtacc actggcatgg tggtggggat tgtcgcagca gcagctctgt
     2461 gcatcctcat cctcctctat gccatgtaca agtacaggaa ccgggatgaa ggttcaagcg
     2521 ctccaccgct cgacggagcc ggagctggcg ctggagctgg agccggagct ggcgggctag
     2581 ccaccatgga cgcacaaaca cgacgacgtg agcgtcgcgc tgagaaacaa gctcaatgga
     2641 aagctgcaaa cctcgagcca ccgctcgacg gagccggagc tggcgctgga gctggagccg
     2701 gagctggcgg tctagccacc ccaccgagcg ctgtggatga gagtcgaaac tacatcagta
     2761 actcagcaca gtccaatggg gctgtggtca aggagaagca gcccagcagt gcgaaaagcg
     2821 ccaacaaaaa caagaagaac aaggataagg agtattatgt ctgacgatat caagcttatc
     2881 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2941 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3001 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3061 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3121 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3181 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3241 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     3301 ctcgcctatg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3361 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3421 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     3481 cgagcgctgc tcgagctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     3541 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3601 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3661 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3721 ctggggagtg tctagactgc agagggccct gcgtatgagt gcaagtgggt tttaggacca
     3781 ggatgaggcg gggtgggggt gcctacctga cgaccgaccc cgacccactg gacaagcacc
     3841 caacccccat tccccaaatt gcgcatcccc tatcagagag ggggagggga aacaggatgc
     3901 ggcgaggcgc gtgcgcactg ccagcttcag caccgcggac agtgccttcg cccccgcctg
     3961 gcggcgcgcg ccaccgccgc ctcagcactg aaggcgcgct gacgtcactc gccggtcccc
     4021 cgcaaactcc ccttcccggc caccttggtc gcgtccgcgc cgccgccggc ccagccggac
     4081 cgcaccacgc gaggcgcgag ataggggggc acgggcgcga ccatctgcgc tgcggcgccg
     4141 gcgactcagc gctgcctcag tctgcggtgg gcagcggagg agtcgtgtcg tgcctgagag
     4201 cgcagtcgag aacgtacgtt ccctaagtcc aactactaaa ctgggctaag tccaactact
     4261 aaactgggcc ctgaagaagg gcccatatag ggccctgaag aagggcccta tcgaggatat
     4321 tatctcgagc aattgctagt acgatgcata acttgtttat tgcagcttat aatggttaca
     4381 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4441 gtggtttgtc caaactcatc aatgtatctt acatgctggg gaggtaacca cgtgcggacc
     4501 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     4561 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     4621 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     4681 catctgtgcg gtattcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     4741 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4801 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4861 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4921 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4981 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5041 tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     5101 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5161 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     5221 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     5281 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     5341 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     5401 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5461 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5521 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5581 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5641 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5701 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5761 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5821 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5881 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5941 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6001 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6061 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6121 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6181 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6241 tggctggttt attgctgata aatctggagc cggtgagcgt ggaagccgcg gtatcattgc
     6301 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6361 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6421 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6481 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6541 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6601 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6661 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6721 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     6781 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6841 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6901 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6961 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7021 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7081 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7141 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7201 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//
