LOCUS       hSyn-PostSynBar-        7631 bp DNA     circular SYN 01-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Stanford University
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /label=ITR
                     /label=ITR(1)
                     /note="color: #00ffff"
     misc_feature    151..628
                     /label=synapsin promoter
                     /note="color: #4eff51"
     misc_feature    642..646
                     /label=Kozak consensus
                     /note="color: #ffd92b"
     CDS             648..3038
                     /codon_start=1
                     /label=NL1
                     /note="color: #993366"
                     /translation="MSALLILALVGAAVADYKDDDDKLAAANSSIDLQKLDDVDPLVTT
                     NFGKIRGIKKELNNEILGPVIQFLGVPYAAPPTGEHRFQPPEPPSPWSDIRNATQFAPV
                     CPQNIIDGRLPEVMLPVWFTNNLDVVSSYVQDQSEDCLYLNIYVPTEDVKRISKECARK
                     PGKKICRKGDIRDSGGPKPVMVYIHGGSYMEGTGNLYDGSVLASYGNVIVITVNYRLGV
                     LGFLSTGDQAAKGNYGLLDLIQALRWTSENIGFFGGDPLRITVFGSGAGGSCVNLLTLS
                     HYSEKGLFQRAIAQSGTALSSWAVSFQPAKYARILATKVGCNVSDTVELVECLQKKPYK
                     ELVDQDVQPARYHIAFGPVIDGDVIPDDPQILMEQGEFLNYDIMLGVNQGEGLKFVENI
                     VDSDDGVSASDFDFAVSNFVDNLYGYPEGKDVLRETIKFMYTDWADRHNPETRRKTLLA
                     LFTDHQWVAPAVATADLHSNFGSPTYFYAFYHHCQTDQVPAWADAAHGDEVPYVLGIPM
                     IGPTELFPCNFSKNDVMLSAVVMTYWTNFAKTGDPNQPVPQDTKFIHTKPNRFEEVAWT
                     RYSQKDQLYLHIGLKPRVKEHYRANKVNLWLELVPHLHNLNDISQTRGGSGGGSGGGRD
                     HMVLHEYVNAAGITGGGSGGGSGGTRYTSTTTKVPSTDITLRPTRKNSTPVTSAFPTAK
                     QDDPKQQPSPFSVDQRDYSTELSVTIAVGASLLFLNILAFAALYYKKDKRRHDVHRRCS
                     PQRTTTNDLTHAPEEEIMSLQMKHTDLDHECESIHPHEVVLRTA"
     CDS             648..692
                     /codon_start=1
                     /product="leader sequence from mouse preprotrypsin"
                     /label=preprotrypsin leader
                     /note="color: #993366
                     Cleavage site after base 692"
                     /translation="MSALLILALVGAAVA"
     CDS             693..716
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     regulatory      1208..1217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     misc_feature    2511..2546
                     /label=Ser/Gly Linker
                     /note="color: #a6acb3"
     CDS             2547..2594
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /note="color: #05fd14"
                     /translation="RDHMVLHEYVNAAGIT"
     misc_feature    2595..2630
                     /label=Ser/Gly Linker
                     /note="color: #a6acb3"
     CDS             3039..3065
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             3072..3098
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     CDS             3108..3305
                     /codon_start=1
                     /label=NL1
                     /note="color: #993366"
                     /translation="CPPDYTLAMRRSPDDVPLMTPNTITMIPNTIPGIQPLHTFNTFTG
                     GQNNTLPHPHPHPHSHSTTRV"
     misc_feature    3306..3371
                     /label=lambda peptide
                     /note="color: #a6acb3"
     CDS             3372..3374
                     /codon_start=1
                     /label=NL1
                     /note="color: #993366"
                     /translation=""
     misc_feature    3389..3997
                     /label=WPRE
                     /note="color: #0000ff"
     misc_feature    4012..4489
                     /label=synapsin promoter
                     /note="color: #4eff51"
     misc_feature    4503..4613
                     /label=2BoxB
                     /note="color: #a6acb3"
     misc_feature    4620..4627
                     /label=barcode region
                     /note="color: #a6acb3"
     misc_feature    4635..4866
                     /label=PolyA
                     /note="color: #ff2549"
     misc_feature    4895..5035
                     /label=ITR
                     /note="color: #00ffff"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc cgccaccatg tctgcacttc
      661 tgatcctagc tcttgttgga gctgcagttg ctgactacaa agacgatgac gacaagcttg
      721 cggccgcgaa ttcatcgata gatctgcaaa agttggatga tgtagaccca ttggttacta
      781 ctaactttgg caaaataagg ggaattaaga aagaactcaa taatgaaatt ttggggcctg
      841 tcattcagtt tcttggggtt ccatatgctg ctccaccaac aggagaacat cgatttcagc
      901 ctccagaacc accatctccc tggtctgata tccggaatgc cactcagttt gctcctgtat
      961 gtccccagaa tatcattgat ggcagattgc ctgaagttat gcttcctgtg tggttcacta
     1021 ataacttgga tgtggtttcc tcatatgtgc aagaccagag tgaagactgt ctatacttaa
     1081 acatctatgt cccaactgaa gatgtaaaaa gaatatccaa ggaatgtgcc agaaaacccg
     1141 gcaagaaaat atgtagaaaa ggagatattc gggacagtgg gggtcccaaa ccagtgatgg
     1201 tgtacatcca tggtggctct tacatggaag gtactggaaa tctatatgat gggagtgtgt
     1261 tggcaagcta cggtaatgtg atcgtcatca cagtcaacta tcggcttggg gtacttggct
     1321 tcttgagcac aggggatcag gctgccaaag gaaactacgg gctccttgac ctcatccagg
     1381 ccctcagatg gaccagcgag aacattgggt tctttggtgg tgaccccttg cgaatcaccg
     1441 tgtttggatc aggcgctggg ggttcatgtg tcaatctgct gactttatcc cattattctg
     1501 aaaaaggact ttttcaacga gcaatagctc agagtggaac agccctttcc agctgggctg
     1561 ttagtttcca gcctgcaaaa tacgctagaa ttctagccac aaaagttggc tgcaatgtgt
     1621 cagatacagt agagttagta gaatgcctgc agaagaagcc ttacaaagaa cttgttgatc
     1681 aagatgttca accagcccga taccacatag cctttggacc tgtgatcgat ggtgatgtaa
     1741 taccagatga ccctcagata ctgatggaac aaggagagtt cctcaattat gatatcatgt
     1801 taggagttaa ccaaggggaa gggttgaagt ttgtggaaaa catagtagat agtgatgatg
     1861 gtgtatcagc cagtgatttt gactttgctg tctctaattt tgttgataat ttatatggat
     1921 atcctgaagg caaagatgtt ttgagagaaa ccattaaatt catgtatact gactgggctg
     1981 atcgccataa tcctgaaact agaaggaaga cattgttggc tttgtttaca gaccatcagt
     2041 gggtagcacc cgctgtggct acagcagacc ttcactcgaa ctttggctca cctacatact
     2101 tctatgcctt ttatcatcat tgccaaacag accaagttcc agcttgggct gatgcagctc
     2161 atggggatga ggttccctat gtattgggaa tccccatgat tggccctaca gagttatttc
     2221 cttgcaattt ctccaagaat gatgtgatgt tgagtgctgt agtaatgaca tactggacga
     2281 attttgctaa aaccggtgac ccaaatcaac cagttcctca agacacaaaa ttcattcata
     2341 ccaaacccaa ccgctttgaa gaagtagcat ggaccagata ttcccagaaa gaccaacttt
     2401 atctccatat tggattaaaa ccgagagtta aagaacatta cagagccaat aaggtaaatc
     2461 tctggttgga gctggtacct catctgcata atctcaatga catttctcag acgcgtggag
     2521 gttcaggtgg aggttcagga ggaggaagag atcacatggt tttgcacgaa tacgtgaatg
     2581 ctgcaggaat tacaggtgga ggttctggag gtggttctgg aggtacgcgt tatacctcga
     2641 caacaactaa agtgccatca acagacatca ctctcagacc tacaaggaaa aattccactc
     2701 cagtcacatc agcctttccc actgccaagc aggatgatcc caaacaacaa ccaagtccct
     2761 tctcggtaga tcaaagggac tactccacag agctaagtgt cacaatcgca gtgggagcct
     2821 ctctgctgtt tctgaacatc ttggcttttg cagccctata ctacaagaaa gacaagagga
     2881 gacatgatgt ccaccggagg tgcagccctc agcgcaccac gaccaacgac ctaacccatg
     2941 ctccagaaga ggaaattatg tctctccaaa tgaagcacac tgacttggat cacgagtgtg
     3001 agtccatcca tccacatgag gtggttcttc ggaccgccta cccatacgat gttccagatt
     3061 acgctgctag ctatccttat gacgtgcctg actatgcccg gaccgcctgc cccccagatt
     3121 atactcttgc tatgaggagg tcaccggatg atgttccttt aatgacacct aacaccatca
     3181 caatgattcc caacactata ccagggattc agcccttaca tacattcaac acatttactg
     3241 gaggacagaa taatacactg ccccaccccc accctcaccc ccattcacat tcaacaacca
     3301 gggtaatgga cgcacaaaca cgacgacgtg agcgtcgcgc tgagaaacaa gctcaatgga
     3361 aagctgcaaa ctaggaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     3421 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3481 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     3541 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     3601 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     3661 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     3721 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     3781 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctatgttgcc acctggattc
     3841 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     3901 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     3961 ggatctccct ttgggccgcc tccccgcatc gataccgagc gctgctcgcg agtgtctaga
     4021 ctgcagaggg ccctgcgtat gagtgcaagt gggttttagg accaggatga ggcggggtgg
     4081 gggtgcctac ctgacgaccg accccgaccc actggacaag cacccaaccc ccattcccca
     4141 aattgcgcat cccctatcag agagggggag gggaaacagg atgcggcgag gcgcgtgcgc
     4201 actgccagct tcagcaccgc ggacagtgcc ttcgcccccg cctggcggcg cgcgccaccg
     4261 ccgcctcagc actgaaggcg cgctgacgtc actcgccggt cccccgcaaa ctccccttcc
     4321 cggccacctt ggtcgcgtcc gcgccgccgc cggcccagcc ggaccgcacc acgcgaggcg
     4381 cgagataggg gggcacgggc gcgaccatct gcgctgcggc gccggcgact cagcgctgcc
     4441 tcagtctgcg gtgggcagcg gaggagtcgt gtcgtgcctg agagcgcagt cgagaacgta
     4501 cgttccctaa gtccaactac taaactgggc taagtccaac tactaaactg ggccctgaag
     4561 aagggcccat atagggccct gaagaagggc cctatcgagg atattatctc gagatgcatc
     4621 tagtacgcaa ttggctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc
     4681 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     4741 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     4801 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     4861 tggggaggta accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc
     4921 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     4981 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc
     5041 ctgatgcggt attttctcct tacgcatctg tgcggtattc acaccgcata cgtcaaagca
     5101 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     5161 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     5221 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     5281 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     5341 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5401 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt
     5461 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5521 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     5581 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     5641 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     5701 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     5761 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     5821 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5881 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5941 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     6001 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6061 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6121 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6181 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6241 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6301 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6361 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6421 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6481 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6541 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6601 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6661 gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6721 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6781 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6841 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6901 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6961 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     7021 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7081 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     7141 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7201 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7261 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7321 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7381 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7441 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7501 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7561 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7621 tgctcacatg t
//
