LOCUS       hSyn-Cre-hSyn-Pr        5835 bp DNA     circular SYN 01-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /label=ITR
                     /label=ITR(1)
                     /note="color: #00ffff"
     misc_feature    151..628
                     /label=synapsin promoter
                     /note="color: #4eff51"
     CDS             642..1670
                     /codon_start=1
                     /label=Cre
                     /note="color: #ffa632"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     misc_feature    1680..1925
                     /label=WPRE3
                     /note="/ApEinfo_fwdcolor=#0000ff"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=green"
                     /note="/vntifkey=21"
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    1928..1971
                     /label=SV40 UPstream Element
                     /label=SV40\UPstream\Element
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=green"
                     /note="/vntifkey=21"
                     /note="color: #3366cc"
     misc_feature    1975..2094
                     /label=SV40 Late polyA
                     /label=SV40\Late\polyA
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=green"
                     /note="/vntifkey=21"
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    2052^2053
                     /label=delete A
                     /note="color: #a6acb3"
     misc_feature    2095..2099
                     /label=PolyA
                     /note="color: #ff2549"
     misc_feature    2107..2584
                     /label=synapsin promoter
                     /note="color: #4eff51"
     misc_feature    2538..2584
                     /label=hSyn exon1
                     /note="color: #a6acb3"
     misc_feature    2597..2771
                     /label=PPIB
                     /note="color: #ff0000"
     misc_feature    2784..2804
                     /label=RPL7-5'
                     /note="color: #a6acb3"
     misc_feature    2805..2915
                     /label=2BoxB
                     /note="color: #a6acb3"
     protein_bind    2852..2870
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
                     /note="color: #31849b"
     protein_bind    2876..2894
                     /label=boxB
                     /bound_moiety="lambda-N peptide"
                     /note="lambda N peptide binding site consisting of an RNA 
                     hairpin within the N-utliization (nut) site of the lambda 
                     bacteriophage transcript (Tan and Frankel, 1995)"
                     /note="color: #31849b"
     misc_feature    2916..2937
                     /label=10X capture seq1
                     /note="color: #3366ff"
     polyA_signal    2938..3059
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #ff0000"
     misc_feature    3060..3070
                     /label=PolyA
                     /note="color: #ff2549"
     misc_feature    3099..3239
                     /label=ITR
                     /note="color: #00ffff"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc catgtccaat ttactgaccg
      661 tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc
      721 tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa atgcttctgt
      781 ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag
      841 aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa
      901 ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac
      961 caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg
     1021 ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt
     1081 cactcatgga aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg
     1141 cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac
     1201 gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg
     1261 caggtgtaga gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg
     1321 tctctggtgt agctgatgat ccgaataact acctgttttg ccgggtcaga aaaaatggtg
     1381 ttgccgcgcc atctgccacc agccagctat caactcgcgc cctggaaggg atttttgaag
     1441 caactcatcg attgatttac ggcgctaagg atgactctgg tcagagatac ctggcctggt
     1501 ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga gtttcaatac
     1561 cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac tatatccgta
     1621 acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat tagactagta
     1681 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     1741 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     1801 tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact
     1861 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     1921 cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca tctagcttta
     1981 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     2041 ttaacaacaa cattgcattc attttatgtt tcaggttcag ggggagatgt gggagcatgc
     2101 tggggagtgt ctagactgca gagggccctg cgtatgagtg caagtgggtt ttaggaccag
     2161 gatgaggcgg ggtgggggtg cctacctgac gaccgacccc gacccactgg acaagcaccc
     2221 aacccccatt ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg
     2281 gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg
     2341 cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc
     2401 gcaaactccc cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc
     2461 gcaccacgcg aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg
     2521 cgactcagcg ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc
     2581 gcagcaattg gagaccggag aagccctttg ccatcgccaa ggagtagggc acagggacat
     2641 ctttctttga gtgaccgtct gtgcaggccc tgtagtccgc cacagggctc tgagctgcac
     2701 tggccccggt gctggcatct ggtggagcgg acccactccc ctcacattcc acaggcccat
     2761 ggactcactt tggtctcatg catcctcttt ttccggctgg aaccttccct aagtccaact
     2821 actaaactgg gctaagtcca actactaaac tgggccctga agaagggccc atatagggcc
     2881 ctgaagaagg gccctatcga ggatattatc tcgaggcttt aaggccggtc ctagcaaaac
     2941 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3001 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttac
     3061 atgctgggga ggtaaccacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
     3121 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     3181 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     3241 gcgcctgatg cggtattttc tccttacgca tctgtgcggt attcacaccg catacgtcaa
     3301 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     3361 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     3421 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     3481 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     3541 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     3601 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     3661 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     3721 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     3781 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     3841 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     3901 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     3961 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     4021 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4081 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4141 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4201 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4261 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4321 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     4381 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     4441 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     4501 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     4561 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     4621 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     4681 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     4741 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     4801 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     4861 gtgagcgtgg aagccgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     4921 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     4981 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5041 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5101 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5161 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5221 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5281 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     5341 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     5401 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     5461 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     5521 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     5581 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     5641 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     5701 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     5761 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     5821 cttttgctca catgt
//
