LOCUS       hSyn-Cre-hSyn-Po        5866 bp DNA     circular SYN 01-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5866
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /label=ITR
                     /label=ITR(1)
                     /note="color: #00ffff"
     misc_feature    151..628
                     /label=synapsin promoter
                     /note="color: #4eff51"
     CDS             642..1670
                     /codon_start=1
                     /label=Cre
                     /note="color: #ffa632"
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     misc_feature    1680..1925
                     /label=WPRE3
                     /note="/ApEinfo_fwdcolor=#0000ff"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=green"
                     /note="/vntifkey=21"
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    1928..1971
                     /label=SV40 UPstream Element
                     /label=SV40\UPstream\Element
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=green"
                     /note="/vntifkey=21"
                     /note="color: #3366cc"
     misc_feature    1975..2094
                     /label=SV40 Late polyA
                     /label=SV40\Late\polyA
                     /note="/ApEinfo_fwdcolor=cyan"
                     /note="/ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /note="/ApEinfo_revcolor=green"
                     /note="/vntifkey=21"
                     /note="color: #66cc99; direction: RIGHT"
     misc_feature    2052^2053
                     /label=delete A
                     /note="color: #a6acb3"
     misc_feature    2095..2099
                     /label=PolyA
                     /note="color: #ff2549"
     misc_feature    2107..2584
                     /label=synapsin promoter
                     /note="color: #4eff51"
     misc_feature    2591..2730
                     /label=dapB 3zz target
                     /note="color: #a6acb3"
     misc_feature    2737..2847
                     /label=2BoxB
                     /note="color: #a6acb3"
     misc_feature    2737..2847
                     /label=barII
                     /note="color: #a6acb3"
     misc_feature    2848..2869
                     /label=10X capture seq2
                     /note="color: #ff6600"
     misc_feature    2870..3101
                     /label=PolyA
                     /note="color: #ff2549"
     misc_feature    3130..3270
                     /label=ITR
                     /note="color: #00ffff"
     misc_feature    4186..5046
                     /label=AmpR
                     /note="color: #a6acb3"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaca gtgtctagac tgcagagggc cctgcgtatg
      181 agtgcaagtg ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga
      241 ccccgaccca ctggacaagc acccaacccc cattccccaa attgcgcatc ccctatcaga
      301 gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg
      361 gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc
      421 gctgacgtca ctcgccggtc ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg
      481 cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg
      541 cgaccatctg cgctgcggcg ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg
      601 aggagtcgtg tcgtgcctga gagcgcagtc gagaaggatc catgtccaat ttactgaccg
      661 tacaccaaaa tttgcctgca ttaccggtcg atgcaacgag tgatgaggtt cgcaagaacc
      721 tgatggacat gttcagggat cgccaggcgt tttctgagca tacctggaaa atgcttctgt
      781 ccgtttgccg gtcgtgggcg gcatggtgca agttgaataa ccggaaatgg tttcccgcag
      841 aacctgaaga tgttcgcgat tatcttctat atcttcaggc gcgcggtctg gcagtaaaaa
      901 ctatccagca acatttgggc cagctaaaca tgcttcatcg tcggtccggg ctgccacgac
      961 caagtgacag caatgctgtt tcactggtta tgcggcggat ccgaaaagaa aacgttgatg
     1021 ccggtgaacg tgcaaaacag gctctagcgt tcgaacgcac tgatttcgac caggttcgtt
     1081 cactcatgga aaatagcgat cgctgccagg atatacgtaa tctggcattt ctggggattg
     1141 cttataacac cctgttacgt atagccgaaa ttgccaggat cagggttaaa gatatctcac
     1201 gtactgacgg tgggagaatg ttaatccata ttggcagaac gaaaacgctg gttagcaccg
     1261 caggtgtaga gaaggcactt agcctggggg taactaaact ggtcgagcga tggatttccg
     1321 tctctggtgt agctgatgat ccgaataact acctgttttg ccgggtcaga aaaaatggtg
     1381 ttgccgcgcc atctgccacc agccagctat caactcgcgc cctggaaggg atttttgaag
     1441 caactcatcg attgatttac ggcgctaagg atgactctgg tcagagatac ctggcctggt
     1501 ctggacacag tgcccgtgtc ggagccgcgc gagatatggc ccgcgctgga gtttcaatac
     1561 cggagatcat gcaagctggt ggctggacca atgtaaatat tgtcatgaac tatatccgta
     1621 acctggatag tgaaacaggg gcaatggtgc gcctgctgga agatggcgat tagactagta
     1681 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     1741 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     1801 tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact
     1861 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     1921 cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca tctagcttta
     1981 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
     2041 ttaacaacaa cattgcattc attttatgtt tcaggttcag ggggagatgt gggagcatgc
     2101 tcgcgagtgt ctagactgca gagggccctg cgtatgagtg caagtgggtt ttaggaccag
     2161 gatgaggcgg ggtgggggtg cctacctgac gaccgacccc gacccactgg acaagcaccc
     2221 aacccccatt ccccaaattg cgcatcccct atcagagagg gggaggggaa acaggatgcg
     2281 gcgaggcgcg tgcgcactgc cagcttcagc accgcggaca gtgccttcgc ccccgcctgg
     2341 cggcgcgcgc caccgccgcc tcagcactga aggcgcgctg acgtcactcg ccggtccccc
     2401 gcaaactccc cttcccggcc accttggtcg cgtccgcgcc gccgccggcc cagccggacc
     2461 gcaccacgcg aggcgcgaga taggggggca cgggcgcgac catctgcgct gcggcgccgg
     2521 cgactcagcg ctgcctcagt ctgcggtggg cagcggagga gtcgtgtcgt gcctgagagc
     2581 gcagatgcat atctgtgtaa atgaaagcat ctgactcaac aggcatcaca tcagataatt
     2641 tttgctgatc gtatgtatgg tctatggccc ctacaaggtc aaaatgtggt gttcgttctg
     2701 ccaatttaac agcttcctgc cccattcttc caattgttcc ctaagtccaa ctactaaact
     2761 gggctaagtc caactactaa actgggccct gaagaagggc ccatataggg ccctgaagaa
     2821 gggccctatc gaggatatta tctcgaggct cacctattag cggctaaggc tagagctcgc
     2881 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     2941 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     3001 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     3061 aagggggagg attgggaaga caatagcagg catgctgggg aggtaaccac gtgcggaccg
     3121 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     3181 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     3241 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     3301 atctgtgcgg tattcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     3361 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3421 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3481 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3541 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     3601 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     3661 ggaacaacac tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     3721 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     3781 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     3841 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     3901 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     3961 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     4021 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4081 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4141 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4201 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4261 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4321 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4381 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4441 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4501 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4561 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4621 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4681 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4741 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4801 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4861 ggctggttta ttgctgataa atctggagcc ggtgagcgtg gaagccgcgg tatcattgca
     4921 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4981 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5041 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5101 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5161 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5221 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5281 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5341 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     5401 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5461 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5521 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     5581 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5641 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5701 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5761 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5821 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
