LOCUS       sCAG-DIO-oScarle        5012 bp DNA     circular SYN 01-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    22..142
                     /label=left full-length ITR
                     /note="left full-length ITR"
                     /note="color: #0080ff; direction: RIGHT"
     misc_feature    167..415
                     /label=CMV early enhancer
                     /note="CMV early enhancer"
                     /note="color: #ff00ff"
     misc_feature    417..1103
                     /label=chicken B actin promoter
                     /note="chicken B actin promoter"
                     /note="color: #ff6489"
     misc_feature    706..707
                     /label=original pAAV-CAG-GFP sequence had an A
                     /note="original pAAV-CAG-GFP sequence had an A between the 
                     G and C, found to be not there by sequencing 05/15"
                     /note="color: #0080ff"
     protein_bind    1110..1143
                     /label=lox2272
                     /note="/color=#944603"
                     /note="color: #31849b"
     protein_bind    1194..1227
                     /label=loxP
                     /note="/color=#fd3434"
                     /note="color: #31849b"
     CDS             complement(1239..1925)
                     /codon_start=1
                     /label=oScarlet3
                     /note="color: #993366"
                     /translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWDRVMNFEDGGA
                     VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKM
                     ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
                     TGGSGGS"
     misc_feature    1927..1931
                     /label=Kozak consensus
                     /note="/color=#68e66d"
                     /note="/direction=NONE"
                     /note="color: #a6acb3"
     protein_bind    complement(1938..1971)
                     /label=lox2272
                     /note="/color=#db6901"
                     /note="color: #31849b; direction: LEFT"
     protein_bind    complement(2022..2055)
                     /label=loxP
                     /note="/color=#eda481"
                     /note="color: #31849b; direction: LEFT"
     misc_feature    2085..2218
                     /label=SV40 polyA
                     /note="SV40 polyA"
                     /note="color: #999999"
     misc_feature    complement(2224..2336)
                     /label=right ITR delta trs
                     /note="right ITR delta trs"
                     /note="color: #0080ff; direction: LEFT"
     CDS             2962..3822
                     /codon_start=1
                     /label=Ampicillin resistance
                     /note="Ampicillin resistance"
                     /note="color: #ffff00"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aaagcttccc ggggggatct gggccactcc ctctctgcgc gctcgctcgc tcactgaggc
       61 cgggcgacca aaggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc
      121 aactccatca ctaggggttc ctggaggggt ggagtcgtga cctaggccat tgacgtcaat
      181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      241 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtcga
      421 ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac ccccaatttt
      481 gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg ggggggggcg
      541 cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg gcgaggcgga gaggtgcggc
      601 ggcagccaat cagagcggcg cgctccgaaa gtttcctttt atggcgaggc ggcggcggcg
      661 gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc gctgcgcgct gccttcgccc
      721 cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg ctctgactga ccgcgttact
      781 cccacaggtg agcgggcggg acggcccttc tcctccgggc tgtaattagc gcttggttta
      841 atgacggctt gtttcttttc tgtggctgcg tgaaagcctt gaggggctcc gggagggccc
      901 tttgtgcggg gggagcggct cggggctgtc cgcgggggga cggctgcctt cgggggggac
      961 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
     1021 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
     1081 ctcatcattt tggcaaagaa ttgggatcca taacttcgta taggatactt tatacgaagt
     1141 tatgcagaat ggtagctgga ttgtagctgc tattagcaat atgaaacctc ttaataactt
     1201 cgtatagcat acattatacg aagttatggc gcgcctcagg aaccaccaga ccccccggtg
     1261 ctatgccgag cgacgctgcg ctcgtactgc tccaccactg tataatcttc attatgtgaa
     1321 gttatgtcca gcttcctgtc aatgttgaag gctcctggca tctggacagg tttctttgcg
     1381 cggtaggtag ttttaaagtc tgccaggtat cggccaccat cttttaatcg caacgccatt
     1441 ttgatatccc ctttcaaaac tacatcctcg ggatatagtc tttccgtgga ggcctcccag
     1501 cccatagtct tcttctgcat cactggtcca tctgggggga agttggtgcc ccgaagcttg
     1561 accttgtaga tgagcgttcc atcctccagt gaggtgtcct gggccacaga tactgcccct
     1621 ccgtcttcaa agttcatgac acggtcccac ttgaatcctt ctgggaaact ttgtttccag
     1681 tagtccggaa tgtcagcagg gtgctttgta aaagctctag agccatacat gaactgggga
     1741 ctcaggatat cccagctgaa aggcagagga ccccccttgg taaccctgag cttggctgtt
     1801 tgagtgccct catagggcct cccttctccc tcaccttcaa tctcaaattc gtggccattc
     1861 atggagccct ccatgtgcac tttgaatctc ataaactcct tgatcacagc ttctgtgctg
     1921 tccatggtgg cgctagcata acttcgtata aagtatccta tacgaagtta tttgccttaa
     1981 cccagaaatt atcactgtta ttctttagaa tggtgcaaag aataacttcg tataatgtat
     2041 gctatacgaa gttatgcggc cgctaggcct cacctgcgat ctcgatgctt tatttgtgaa
     2101 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     2161 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaact
     2221 agtccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     2281 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggacaga
     2341 tccgggcccg catgcgtcga caattcactg gccgtcgttt tacaacgtcg tgactgggaa
     2401 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     2461 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     2521 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     2581 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca
     2641 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     2701 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     2761 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     2821 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2881 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2941 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3001 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3061 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     3121 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     3181 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     3241 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     3301 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     3361 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     3421 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     3481 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3541 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3601 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3661 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     3721 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3781 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3841 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3901 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3961 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4021 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4081 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     4141 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     4201 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     4261 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     4321 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4381 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4441 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4501 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4561 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4621 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4681 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4741 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     4801 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     4861 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     4921 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     4981 gaccatgatt acgccaagct ctcgagatct ag
//
