LOCUS       pAAV[Exp]-EF1A>{WAChR_1f-G-mScr3-SE}:WPRE3]      6546 bp    DNA     circular     2025-07-25 
FEATURES             Location/Qualifiers
     ITR             1..130
                     /application_notes="Allows rescue of virus from recombinant plasmid and replication of the viral genome; this ITR is identical to that of the wild-type AAV2 genome."
                     /description="AAV 5' inverted terminal repeat (functional equivalent of wild-type 5' ITR)"
                     /label="5' ITR"
                     /note="color: #ce0084; direction: RIGHT"
                     /official_designation="5' ITR"
                     /uuid="6d343a478711450d8e5c28ceb27e5b84"
     Promoter        158..1336
                     /application_notes="Strong promoter."
                     /description="Human eukaryotic translation elongation factor 1 alpha1 promoter"
                     /full_name="Human eukaryotic translation elongation factor 1 alpha1 promoter"
                     /label="EF1A"
                     /note="color: #6d3ed8; direction: RIGHT"
                     /official_designation="EF1A"
                     /uuid="69c052aac71a4f49bab5b7ae1f46ace8"
     Miscellaneous   1361..1366
                     /application_notes="Facilitates translation initiation of ATG start codon downstream of the Kozak sequence."
                     /description="Kozak translation initiation sequence"
                     /full_name="Kozak"
                     /label="Kozak"
                     /note="color: #0375bb; direction: RIGHT"
                     /official_designation="Kozak"
                     /uuid="5f18b25a1234443494a618363f09f052"
     CDS             1367..2299
                     /label="WAChR-1f"
     CDS             2309..2368
                     /label="kir2.1-golgi-trafficking-signal"
     CDS             2369..3055
                     /label="mScarlet3"
     CDS             3056..3250
                     /label="kv2.1-soma-targeting-signal-peptide"
     CDS             3251..3271
                     /label="kir2.1-C-term-TS-and-ER-export"
     Miscellaneous   3305..3552
                     /application_notes="Contained only minimal gamma and alpha subunits of WPRE. Slightly weaker effective compared to WPRE."
                     /description="Truncated short version of woodchuck hepatitis virus posttranscriptional regulatory element (248 bp)"
                     /label="WPRE3"
                     /note="color: #a949ca; direction: RIGHT"
                     /official_designation="WPRE3"
                     /uuid="f2d2a70140634b0189319031f36c6213"
     Regulatory_Elem 3583..3790
                     /application_notes="Allows transcription termination and polyadenylation of mRNA transcribed by Pol II RNA polymerase."
                     /description="Bovine growth hormone polyadenylation signal"
                     /full_name="Bovine growth hormone polyadenylation"
                     /label="BGH pA"
                     /note="color: #946a00; direction: RIGHT"
                     /official_designation="BGH pA"
                     /uuid="4a09734ca6c745b3aadc757c1638b651"
     ITR             complement(3798..3927)
                     /application_notes="Allows rescue of virus from recombinant plasmid and replication of the viral genome; this ITR is identical to that of the wild-type AAV2 genome."
                     /description="AAV 3' inverted terminal repeat"
                     /label="3' ITR"
                     /note="color: #512bbd; direction: LEFT"
                     /official_designation="3' ITR"
                     /uuid="a5d684b85c014f76a7b6d8e97945f0fb"
     CDS             4855..5715
                     /application_notes="Allows E. coli to be resistant to ampiciIIin."
                     /description="AmpiciIIin resistance gene"
                     /full_name="Ampicillin resistance gene"
                     /label="AmpiciIIin"
                     /note="color: #d84e4e; direction: RIGHT"
                     /official_designation="Ampicillin"
                     /uuid="5bcc09480c0e4c07b95ddccd8242d769"
     Rep_origin      5886..6474
                     /application_notes="Facilitates plasmid replication in E. coli; regulates high-copy plasmid number (500-700)."
                     /description="pUC origin of replication"
                     /full_name="pUC origin of replication"
                     /label="pUC ori"
                     /note="color: #1f36a9; direction: RIGHT"
                     /official_designation="pUC ori"
                     /uuid="b978ad4ff785460db448caf63b728237"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tctagacaac tttgtataga aaagttgggc tccggtgccc gtcagtgggc
      181 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      241 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      301 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      361 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      421 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      481 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      541 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      601 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      661 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      721 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      781 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      841 ggggtagtct caagctggcc ggcctgctct ggtgcctggt ctcgcgccgc cgtgtatcgc
      901 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      961 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     1021 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1081 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1141 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1201 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1261 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1321 atttcaggtg tcgtgacaag tttgtacaaa aaagcaggct gccaccatgt ctgcctgccc
     1381 tacaccccct actgtggtgg cccccactga ccagtctccc aaccctaatg gaggcaccac
     1441 ctcctgtgcc caggctgccc ctagccccca gatgagcatg aactacaccc tgctggccac
     1501 cattgatggc ttctctgctg tgatcctgtt gggctcaggg gtggtgttct ggtactacag
     1561 ctggaggggc acaggctttg gagcccaccc tcaccccaag agaaggcccc tggagctgaa
     1621 caccctgagt gctgccattg cctttttgac gggattgatg tatgtggcca agacctctga
     1681 ctttgccaga gacagcacct ctgagttctc attaaatcga tactggtaca gtgactacat
     1741 tgtgacctgc cccttcattg tgatggatct gtgttttacg gtggatgtgc cctataagct
     1801 ggccttcaca ggcagcacag ctgtcctgct ggtgattgcc ctggccagct ttattgtgag
     1861 aagccctgag aagtacatct actttgccat tggctgcacc ctgttcagca tcttcttcta
     1921 cttctttttc aatgaggtga gaaaaagaat caggtatgtg cctgactgtg ccaagaagga
     1981 cctggagaga gccatgttca ttttctttgg cttctggccc atgtttccgg tattatggct
     2041 gctgggcttc cacaccactg gagtgatctc tgaggaggcc tttgccatca tccatgcctt
     2101 cctggacatt acctgcaaga ccatctttgg ggtgttcatg ctgagatgta gactgcacat
     2161 tgaggactat gcctgggatg agctgaagag gcagagacag gagattgatg aggagaacca
     2221 gaagaccctg acagctgctg gggctgatgg agccacagtg atgatgcctg tggaggagga
     2281 tgacccctat gcctctgggg ctgctgccaa gagcaggatc acctctgaag gggagtacat
     2341 ccccctggac cagattgaca tcaatgtgat ggacagcact gaggctgtga tcaaggagtt
     2401 catgaggttc aaggtgcaca tggagggcag catgaatggc catgagtttg agattgaggg
     2461 agaaggagag ggcagaccct atgagggcac ccagacagcc aagctgaagg tgaccaaggg
     2521 aggccccctg cccttcagct gggacatcct gagcccccag ttcatgtatg gcagcagggc
     2581 cttcatcaag cacccagctg acatccctga ctactggaag caaagcttcc ctgagggctt
     2641 caagtgggag agagtgatga tctttgagga tgggggcaca gtgagtgtga cccaggacac
     2701 cagcctggag gatggcaccc tgatctacaa ggtgaagctg agaggaggca acttccctcc
     2761 tgatggccca gtgatgcaga agaggaccat gggctgggag gccagcacag agaggctgta
     2821 ccctgaggat gtggtgctga agggggacat caagatggcc ctgagactga aggatggagg
     2881 caggtacctg gctgatttca agaccaccta caaggccaag aagcctgtcc agatgcctgg
     2941 agccttcaac attgacagaa agctggacat caccagccac aatgaggact acacagtggt
     3001 ggagcagtat gagagatctg tggccaggca ctccacagga ggctctgggg gcagccagag
     3061 ccaacccatc ctgaacacca aggagatggc ccctcagagc aagccccctg aggagctgga
     3121 gatgagcagc atgcccagcc ctgtggcccc actgcctgcc aggacagagg gagtgattga
     3181 catgagaagc atgagctcca ttgacagctt catcagctgt gccacagact tcccagaggc
     3241 caccaggttc ttctgctatg aaaatgaggt ttgaacccag ctttcttgta caaagtggga
     3301 attcataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     3361 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     3421 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg
     3481 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3541 aattccgtgg tggaattcct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     3601 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     3661 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     3721 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagag aatagcaggc
     3781 atgctgggga gggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     3841 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     3901 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
     3961 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
     4021 tgtagcggcg cattaagcgc ggcgggggtg gtggttacgc gcagcgtgac cgctacactt
     4081 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4141 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     4201 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
     4261 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     4321 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
     4381 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     4441 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
     4501 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     4561 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4621 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     4681 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     4741 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4801 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4861 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4921 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4981 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5041 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     5101 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5161 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5221 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5281 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5341 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5401 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5461 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5521 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg aagccgcggt
     5581 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5641 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5701 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5761 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5821 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5881 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5941 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6001 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6061 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgtcctgc
     6541 aggcag
//