LOCUS       pAAV[Exp]-EF1A>W        6552 bp    DNA     circular SYN 01-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene 8.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..130
                     /label=5' ITR
                     /label=nonstandard type: ITR
                     /note="color: #ef6cdf; direction: RIGHT"
                     /note="/application_notes=Allows rescue of virus from 
                     recombinant plasmid and replication of the viral genome; 
                     this ITR is identical to that of the wild-type AAV2 
                     genome."
                     /note="/description=AAV 5' inverted terminal repeat 
                     (functional equivalent of wild-type 5' ITR)"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=6d343a478711450d8e5c28ceb27e5b84"
     promoter        158..1336
                     /label=EF1A
                     /note="color: #c54b7c; direction: RIGHT"
                     /note="/application_notes=Strong promoter."
                     /note="/description=Human eukaryotic translation elongation
                     factor 1 alpha1 promoter"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor 1 alpha1 promoter"
                     /note="/official_designation=EF1A"
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
     CDS             1367..2299
                     /codon_start=1
                     /label=WAChR_1m
                     /translation="MSACPTPPTVVAPTDQSPNPNGGTTSCAQAAPSPQMSMNYTLLAT
                     IDGFSAVILLGSGVVFWYYSWRGTGFGAHPHPKRRPLELNTLSAAIAFLTGLMYVAKTS
                     DFARDSTSEFSLNRYWYLDYIVTCPLIVMDLCFTVDVPYKLAFTGSTAVLLVIALASFI
                     VRSPEKYIYFAIGCTLFSIFFYFFFNEVRKRIRYVPDCAKKDLERAMFIFFGFWPMFPV
                     LWLLGFHTTGVISEEAFAIIHAFLDITCKTIFGVFMLRCRLHIEDYAWDELKRQRQEID
                     EENQKTLTAAGADGATVMMPVEEDDPYASG"
     CDS             2309..2368
                     /codon_start=1
                     /label=kir2.1-golgi-trafficking-signal
                     /translation="KSRITSEGEYIPLDQIDINV"
     CDS             2369..3061
                     /codon_start=1
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWDRVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     CDS             3062..3256
                     /codon_start=1
                     /label=kv2.1-soma-targeting-signal-peptide
                     /translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
                     MSSIDSFISCATDFPEATRF"
     CDS             3257..3277
                     /codon_start=1
                     /label=kir2.1-C-term-TS-and-ER-export
                     /translation="FCYENEV"
     misc_feature    3311..3558
                     /label=WPRE3
     misc_feature    3589..3796
                     /label=BGH pA
                     /label=nonstandard type: Regulatory_Elem
                     /note="color: #46c6ef; direction: RIGHT"
                     /note="/application_notes=Allows transcription termination 
                     and polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Bovine growth hormone polyadenylation 
                     signal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
     misc_feature    complement(3804..3933)
                     /direction=LEFT
                     /label=3' ITR
                     /label=nonstandard type: ITR
                     /note="color: #eda481; direction: LEFT"
                     /note="/application_notes=Allows rescue of virus from 
                     recombinant plasmid and replication of the viral genome; 
                     this ITR is identical to that of the wild-type AAV2 
                     genome."
                     /note="/description=AAV 3' inverted terminal repeat"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=a5d684b85c014f76a7b6d8e97945f0fb"
     CDS             4861..5721
                     /codon_start=1
                     /label=AmpiciIIin
                     /note="color: #da3bb5; direction: RIGHT"
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5892..6480
                     /direction=RIGHT
                     /label=pUC ori
                     /note="color: #e4b930; direction: RIGHT"
                     /note="/application_notes=Facilitates plasmid replication 
                     in E. coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tctagacaac tttgtataga aaagttgggc tccggtgccc gtcagtgggc
      181 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      241 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      301 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      361 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      421 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      481 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      541 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      601 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      661 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      721 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      781 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      841 ggggtagtct caagctggcc ggcctgctct ggtgcctggt ctcgcgccgc cgtgtatcgc
      901 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      961 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     1021 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1081 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1141 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1201 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1261 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1321 atttcaggtg tcgtgacaag tttgtacaaa aaagcaggct gccaccatga gcgcctgtcc
     1381 tacaccccct accgtggtgg cccccacaga tcaaagcccc aatcctaacg gaggcaccac
     1441 ctcttgtgct caggctgccc cttctcctca gatgagcatg aattacaccc tgcttgcaac
     1501 catcgacgga tttagcgccg tgattctgct tggaagcggc gtggtgttct ggtactactc
     1561 ttggagggga accggctttg gagcccaccc ccatcccaag agaagacccc tggaactgaa
     1621 taccctgtct gccgccatcg ccttcttaac cggcctgatg tacgtggcca agaccagcga
     1681 ctttgctagg gacagcacca gcgagtttag cctgaacagg tactggtacc tggactacat
     1741 cgtgacatgc cccctgatcg tgatggacct gtgcttcacc gtggacgtgc cctacaagct
     1801 ggccttcacc ggcagcaccg ccgtgctgct ggtgatcgcc ctcgccagct tcatcgtgag
     1861 gagccccgag aagtacatct acttcgctat cggctgcacc ctgtttagca ttttcttcta
     1921 cttctttttc aacgaggtga gaaagaggat caggtacgtg cccgactgcg ccaagaagga
     1981 cctggagagg gccatgttca tcttcttcgg cttctggccc atgttccccg tgctgtggct
     2041 gctgggcttt cacaccaccg gcgtgatcag cgaggaggcc tttgctatca ttcacgcctt
     2101 cctagacatt acctgcaaga ccattttcgg cgtgttcatg ctgaggtgca gactgcacat
     2161 cgaggactac gcctgggatg agctgaaaag gcagagacag gagatcgacg aggagaatca
     2221 gaaaaccttg accgccgctg gcgccgatgg cgccaccgtg atgatgcccg ttgaagaaga
     2281 cgacccctac gctagcggag cggccgccaa gagcaggatc accagcgagg gcgagtacat
     2341 ccccctggac cagatcgata tcaacgtggt gagcaagggc gaggcagtga tcaaggagtt
     2401 catgcggttc aaggtgcaca tggagggctc catgaacggc cacgagttcg agatcgaggg
     2461 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     2521 tggccccctg cccttctcct gggacatcct gtcccctcag ttcatgtacg gctccagggc
     2581 cttcaccaag caccccgccg acatccccga ctactataag cagtccttcc ccgagggctt
     2641 caagtgggac cgcgtgatga acttcgagga cggcggcgcc gtgaccgtga cccaggacac
     2701 ctccctggag gacggcaccc tgatctacaa ggtgaagctc cgcggcacca acttccctcc
     2761 tgacggcccc gtaatgcaga agaagacaat gggctgggaa gcgtccaccg agcggttgta
     2821 ccccgaggac ggcgtgctga agggcgacat taagatggcc ctgcgcctga aggacggcgg
     2881 gcgctacctg gcggacttca agaccaccta caaggccaag aagcccgtgc agatgcccgg
     2941 cgcctacaac gtggaccgca agttggacat cacctcccac aacgaggact acaccgtggt
     3001 ggaacagtac gaacgctccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     3061 gcagtcccag cccatcctca acaccaagga gatggccccg cagagcaagc ctccagagga
     3121 gctggagatg agcagcatgc ccagccccgt ggcccctctg cccgcacgca cggagggcgt
     3181 catcgacatg cggagcatgt ccagcattga cagcttcatc agctgtgcca cggacttccc
     3241 tgaagccacc agattcttct gctacgagaa cgaggtgtaa acccagcttt cttgtacaaa
     3301 gtgggaattc ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3361 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     3421 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc
     3481 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3541 actgacaatt ccgtggtgga attcctagag ctcgctgatc agcctcgact gtgccttcta
     3601 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     3661 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     3721 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagagaata
     3781 gcaggcatgc tggggagggc cgcaggaacc cctagtgatg gagttggcca ctccctctct
     3841 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3901 ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
     3961 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
     4021 gcgccctgta gcggcgcatt aagcgcggcg ggggtggtgg ttacgcgcag cgtgaccgct
     4081 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4141 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4201 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
     4261 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4321 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
     4381 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4441 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
     4501 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4561 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4621 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4681 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4741 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4801 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4861 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4921 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4981 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5041 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5101 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5161 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5221 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5281 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5341 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5401 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5461 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5521 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtggaagc
     5581 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5641 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5701 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5761 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5821 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5881 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5941 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6001 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6061 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6121 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6181 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6241 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6301 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6361 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6421 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6481 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6541 tcctgcaggc ag
//
