LOCUS       Exported                5842 bp ds-DNA     circular SYN 01-OCT-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 3, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5842
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..130
                     /label=5' ITR
                     /label=nonstandard type: ITR
                     /note="color: #ef6cdf; direction: RIGHT"
                     /note="/application_notes=Allows rescue of virus from 
                     recombinant plasmid and replication of the viral genome; 
                     this ITR is identical to that of the wild-type AAV2 
                     genome."
                     /note="/description=AAV 5' inverted terminal repeat
                     (functional equivalent of wild-type 5' ITR)"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=6d343a478711450d8e5c28ceb27e5b84"
     promoter        158..626
                     /label=SYN1
                     /note="color: #c54b7c; direction: RIGHT"
                     /note="/application_notes=Tissue specificity: Brain. Cell
                     type specificity: Mature neurons."
                     /note="/description=Human synapsin I promoter"
                     /note="/full_name=Human synapsin I promoter"
                     /note="/official_designation=SYN1"
                     /note="/uuid=6f036b2e82244653b8e92867f598e473"
     CDS             657..1589
                     /codon_start=1
                     /label=WAChR_1m
                     /translation="MSACPTPPTVVAPTDQSPNPNGGTTSCAQAAPSPQMSMNYTLLAT
                     IDGFSAVILLGSGVVFWYYSWRGTGFGAHPHPKRRPLELNTLSAAIAFLTGLMYVAKTS
                     DFARDSTSEFSLNRYWYLDYIVTCPLIVMDLCFTVDVPYKLAFTGSTAVLLVIALASFI
                     VRSPEKYIYFAIGCTLFSIFFYFFFNEVRKRIRYVPDCAKKDLERAMFIFFGFWPMFPV
                     LWLLGFHTTGVISEEAFAIIHAFLDITCKTIFGVFMLRCRLHIEDYAWDELKRQRQEID
                     EENQKTLTAAGADGATVMMPVEEDDPYASG"
     CDS             1599..1658
                     /codon_start=1
                     /label=kir2.1-golgi-trafficking-signal
                     /translation="KSRITSEGEYIPLDQIDINV"
     CDS             1659..2351
                     /codon_start=1
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWDRVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     CDS             2352..2546
                     /codon_start=1
                     /label=kv2.1-soma-targeting-signal-peptide
                     /translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
                     MSSIDSFISCATDFPEATRF"
     CDS             2547..2567
                     /codon_start=1
                     /label=kir2.1-C-term-TS-and-ER-export
                     /translation="FCYENEV"
     misc_feature    2601..2848
                     /label=WPRE3
     misc_feature    2879..3086
                     /label=BGH pA
                     /label=nonstandard type: Regulatory_Elem
                     /note="color: #46c6ef; direction: RIGHT"
                     /note="/application_notes=Allows transcription termination
                     and polyadenylation of mRNA transcribed by Pol II RNA 
                     polymerase."
                     /note="/description=Bovine growth hormone polyadenylation 
                     signal"
                     /note="/full_name=Bovine growth hormone polyadenylation"
                     /note="/official_designation=BGH pA"
                     /note="/uuid=4a09734ca6c745b3aadc757c1638b651"
     misc_feature    complement(3094..3223)
                     /label=3' ITR
                     /label=nonstandard type: ITR
                     /note="color: #eda481; direction: LEFT"
                     /note="/application_notes=Allows rescue of virus from 
                     recombinant plasmid and replication of the viral genome; 
                     this ITR is identical to that of the wild-type AAV2 
                     genome."
                     /note="/description=AAV 3' inverted terminal repeat"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=a5d684b85c014f76a7b6d8e97945f0fb"
                     /note="/direction=LEFT"
     CDS             4151..5011
                     /codon_start=1
                     /label=AmpiciIIin
                     /note="color: #da3bb5; direction: RIGHT"
                     /note="/application_notes=Allows E. coli to be resistant to
                     ampiciIIin."
                     /note="/description=AmpiciIIin resistance gene"
                     /note="/full_name=Ampicillin resistance gene"
                     /note="/official_designation=Ampicillin"
                     /note="/uuid=5bcc09480c0e4c07b95ddccd8242d769"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5182..5770
                     /direction=RIGHT
                     /label=pUC ori
                     /note="color: #e4b930; direction: RIGHT"
                     /note="/application_notes=Facilitates plasmid replication
                     in E. coli; regulates high-copy plasmid number (500-700)."
                     /note="/description=pUC origin of replication"
                     /note="/full_name=pUC origin of replication"
                     /note="/official_designation=pUC ori"
                     /note="/uuid=b978ad4ff785460db448caf63b728237"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tctagacaac tttgtataga aaagttgctg cagagggccc tgcgtatgag
      181 tgcaagtggg ttttaggacc aggatgaggc ggggtggggg tgcctacctg acgaccgacc
      241 ccgacccact ggacaagcac ccaaccccca ttccccaaat tgcgcatccc ctatcagaga
      301 gggggagggg aaacaggatg cggcgaggcg cgtgcgcact gccagcttca gcaccgcgga
      361 cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg cctcagcact gaaggcgcgc
      421 tgacgtcact cgccggtccc ccgcaaactc cccttcccgg ccaccttggt cgcgtccgcg
      481 ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga gatagggggg cacgggcgcg
      541 accatctgcg ctgcggcgcc ggcgactcag cgctgcctca gtctgcggtg ggcagcggag
      601 gagtcgtgtc gtgcctgaga gcgcagcaag tttgtacaaa aaagcaggct gccaccatga
      661 gcgcctgtcc tacaccccct accgtggtgg cccccacaga tcaaagcccc aatcctaacg
      721 gaggcaccac ctcttgtgct caggctgccc cttctcctca gatgagcatg aattacaccc
      781 tgcttgcaac catcgacgga tttagcgccg tgattctgct tggaagcggc gtggtgttct
      841 ggtactactc ttggagggga accggctttg gagcccaccc ccatcccaag agaagacccc
      901 tggaactgaa taccctgtct gccgccatcg ccttcttaac cggcctgatg tacgtggcca
      961 agaccagcga ctttgctagg gacagcacca gcgagtttag cctgaacagg tactggtacc
     1021 tggactacat cgtgacatgc cccctgatcg tgatggacct gtgcttcacc gtggacgtgc
     1081 cctacaagct ggccttcacc ggcagcaccg ccgtgctgct ggtgatcgcc ctcgccagct
     1141 tcatcgtgag gagccccgag aagtacatct acttcgctat cggctgcacc ctgtttagca
     1201 ttttcttcta cttctttttc aacgaggtga gaaagaggat caggtacgtg cccgactgcg
     1261 ccaagaagga cctggagagg gccatgttca tcttcttcgg cttctggccc atgttccccg
     1321 tgctgtggct gctgggcttt cacaccaccg gcgtgatcag cgaggaggcc tttgctatca
     1381 ttcacgcctt cctagacatt acctgcaaga ccattttcgg cgtgttcatg ctgaggtgca
     1441 gactgcacat cgaggactac gcctgggatg agctgaaaag gcagagacag gagatcgacg
     1501 aggagaatca gaaaaccttg accgccgctg gcgccgatgg cgccaccgtg atgatgcccg
     1561 ttgaagaaga cgacccctac gctagcggag cggccgccaa gagcaggatc accagcgagg
     1621 gcgagtacat ccccctggac cagatcgata tcaacgtggt gagcaagggc gaggcagtga
     1681 tcaaggagtt catgcggttc aaggtgcaca tggagggctc catgaacggc cacgagttcg
     1741 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     1801 tgaccaaggg tggccccctg cccttctcct gggacatcct gtcccctcag ttcatgtacg
     1861 gctccagggc cttcaccaag caccccgccg acatccccga ctactataag cagtccttcc
     1921 ccgagggctt caagtgggac cgcgtgatga acttcgagga cggcggcgcc gtgaccgtga
     1981 cccaggacac ctccctggag gacggcaccc tgatctacaa ggtgaagctc cgcggcacca
     2041 acttccctcc tgacggcccc gtaatgcaga agaagacaat gggctgggaa gcgtccaccg
     2101 agcggttgta ccccgaggac ggcgtgctga agggcgacat taagatggcc ctgcgcctga
     2161 aggacggcgg gcgctacctg gcggacttca agaccaccta caaggccaag aagcccgtgc
     2221 agatgcccgg cgcctacaac gtggaccgca agttggacat cacctcccac aacgaggact
     2281 acaccgtggt ggaacagtac gaacgctccg agggccgcca ctccaccggc ggcatggacg
     2341 agctgtacaa gcagtcccag cccatcctca acaccaagga gatggccccg cagagcaagc
     2401 ctccagagga gctggagatg agcagcatgc ccagccccgt ggcccctctg cccgcacgca
     2461 cggagggcgt catcgacatg cggagcatgt ccagcattga cagcttcatc agctgtgcca
     2521 cggacttccc tgaagccacc agattcttct gctacgagaa cgaggtgtaa acccagcttt
     2581 cttgtacaaa gtgggaattc ataatcaacc tctggattac aaaatttgtg aaagattgac
     2641 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     2701 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     2761 agttcttgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     2821 gctgttgggc actgacaatt ccgtggtgga attcctagag ctcgctgatc agcctcgact
     2881 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
     2941 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
     3001 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg
     3061 gaagagaata gcaggcatgc tggggagggc cgcaggaacc cctagtgatg gagttggcca
     3121 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     3181 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     3241 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     3301 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggggtggtgg ttacgcgcag
     3361 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
     3421 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3481 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     3541 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3601 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
     3661 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
     3721 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     3781 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     3841 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     3901 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     3961 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     4021 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4081 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4141 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     4201 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     4261 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     4321 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4381 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4441 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4501 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4561 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4621 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4681 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4741 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4801 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4861 gcgtggaagc cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4921 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4981 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     5041 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     5101 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     5161 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     5221 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     5281 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     5341 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5401 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5461 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5521 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5581 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5641 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5701 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5761 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5821 tgctcacatg tcctgcaggc ag
//
