LOCUS       pAAV[Exp]-EF1A>W        6590 bp    DNA     circular SYN 01-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 1, 2025 from SnapGene 8.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    12..141
                     /label=5' ITR
                     /label=nonstandard type: ITR
                     /note="color: #e4b930; direction: RIGHT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows rescue of virus from 
                     recombinant plasmid and replication of the viral genome; 
                     this ITR is identical to that of the wild-type AAV2 
                     genome."
                     /note="/description=AAV 5' inverted terminal repeat 
                     (functional equivalent of wild-type 5' ITR)"
                     /note="/official_designation=5' ITR"
                     /note="/uuid=6d343a478711450d8e5c28ceb27e5b84"
                     /name="5' ITR"
     promoter        169..1347
                     /label=EF1A
                     /note="color: #05696d; direction: RIGHT"
                     /note="/Application"
                     /note="/Full"
                     /note="/References=<a href= target=_blank>Nucl Acids Res. 
                     18:5322 (1990)</a>; <a href= target=_blank>PLoS One. 
                     5:e10611 (2010)</a>"
                     /note="/Subtype=Mammalian ubiquitous promoter"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/ame=Human eukaryotic translation elongation factor 
                     1 alpha-1 promoter"
                     /note="/application_notes=Strong promoter."
                     /note="/description=Human eukaryotic translation elongation
                     factor 1 alpha1 promoter"
                     /note="/full_name=Human eukaryotic translation elongation 
                     factor 1 alpha1 promoter"
                     /note="/name=EF1A"
                     /note="/official_designation=EF1A"
                     /note="/otes=Strong promoter."
                     /note="/uuid=69c052aac71a4f49bab5b7ae1f46ace8"
     CDS             1378..2310
                     /codon_start=1
                     /label=WAChR_1m
                     /translation="MSACPTPPTVVAPTDQSPNPNGGTTSCAQAAPSPQMSMNYTLLAT
                     IDGFSAVILLGSGVVFWYYSWRGTGFGAHPHPKRRPLELNTLSAAIAFLTGLMYVAKTS
                     DFARDSTSEFSLNRYWYLDYIVTCPLIVMDLCFTVDVPYKLAFTGSTAVLLVIALASFI
                     VRSPEKYIYFAIGCTLFSIFFYFFFNEVRKRIRYVPDCAKKDLERAMFIFFGFWPMFPV
                     LWLLGFHTTGVISEEAFAIIHAFLDITCKTIFGVFMLRCRLHIEDYAWDELKRQRQEID
                     EENQKTLTAAGADGATVMMPVEEDDPYASG"
     CDS             2320..2379
                     /codon_start=1
                     /label=kir2.1-golgi-trafficking-signal
                     /translation="KSRITSEGEYIPLDQIDINV"
     CDS             2380..2574
                     /codon_start=1
                     /label=kv2.1-soma-targeting-signal-peptide
                     /translation="QSQPILNTKEMAPQSKPPEELEMSSMPSPVAPLPARTEGVIDMRS
                     MSSIDSFISCATDFPEATRF"
     CDS             2575..2595
                     /codon_start=1
                     /label=kir2.1-C-term-TS-and-ER-export
                     /translation="FCYENEV"
     CDS             2596..2622
                     /codon_start=1
                     /label=HA-tag
                     /translation="YPYDVPDYA"
     CDS             2623..2688
                     /codon_start=1
                     /label=P2A
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     CDS             2689..3381
                     /codon_start=1
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWDRVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     misc_feature    3385..3632
                     /label=WPRE3
     misc_feature    3633..3656
                     /label=attB2
     misc_feature    3687..3894
                     /label=BGH pA
     misc_feature    complement(3902..4042)
                     /direction=LEFT
                     /label=3' ITR
                     /label=nonstandard type: ITR
                     /note="color: #a949ca; direction: LEFT"
                     /note="/__SeqFeature__=True"
                     /note="/__level__=0"
                     /note="/application_notes=Allows rescue of virus from 
                     recombinant plasmid and replication of the viral genome; 
                     this ITR is identical to that of the wild-type AAV2 
                     genome."
                     /note="/description=AAV 3' inverted terminal repeat"
                     /note="/official_designation=3' ITR"
                     /note="/uuid=5b8101593b634e64aa9eef9434216f71"
                     /name="3' ITR"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc ttctagacaa ctttgtatag aaaagttggg ctccggtgcc
      181 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
      241 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      301 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      361 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
      421 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg
      481 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
      541 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
      601 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
      661 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
      721 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
      781 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
      841 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg
      901 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
      961 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
     1021 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     1081 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     1141 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     1201 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     1261 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     1321 ttttttcttc catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg
     1381 agcgcctgtc ctacaccccc taccgtggtg gcccccacag atcaaagccc caatcctaac
     1441 ggaggcacca cctcttgtgc tcaggctgcc ccttctcctc agatgagcat gaattacacc
     1501 ctgcttgcaa ccatcgacgg atttagcgcc gtgattctgc ttggaagcgg cgtggtgttc
     1561 tggtactact cttggagggg aaccggcttt ggagcccacc cccatcccaa gagaagaccc
     1621 ctggaactga ataccctgtc tgccgccatc gccttcttaa ccggcctgat gtacgtggcc
     1681 aagaccagcg actttgctag ggacagcacc agcgagttta gcctgaacag gtactggtac
     1741 ctggactaca tcgtgacatg ccccctgatc gtgatggacc tgtgcttcac cgtggacgtg
     1801 ccctacaagc tggccttcac cggcagcacc gccgtgctgc tggtgatcgc cctcgccagc
     1861 ttcatcgtga ggagccccga gaagtacatc tacttcgcta tcggctgcac cctgtttagc
     1921 attttcttct acttcttttt caacgaggtg agaaagagga tcaggtacgt gcccgactgc
     1981 gccaagaagg acctggagag ggccatgttc atcttcttcg gcttctggcc catgttcccc
     2041 gtgctgtggc tgctgggctt tcacaccacc ggcgtgatca gcgaggaggc ctttgctatc
     2101 attcacgcct tcctagacat tacctgcaag accattttcg gcgtgttcat gctgaggtgc
     2161 agactgcaca tcgaggacta cgcctgggat gagctgaaaa ggcagagaca ggagatcgac
     2221 gaggagaatc agaaaacctt gaccgccgct ggcgccgatg gcgccaccgt gatgatgccc
     2281 gttgaagaag acgaccccta cgctagcgga gcggccgcca agagcaggat caccagcgag
     2341 ggcgagtaca tccccctgga ccagatcgac atcaacgtgc agtcccagcc catcctcaac
     2401 accaaggaga tggccccgca gagcaagcct ccagaggagc tggagatgag cagcatgccc
     2461 agccccgtgg cccctctgcc cgcacgcacg gagggcgtca tcgacatgcg gagcatgtcc
     2521 agcattgaca gcttcatcag ctgtgccacg gacttccctg aagccaccag attcttctgc
     2581 tacgagaacg aggtgtaccc atacgatgtt ccagattacg ctggcagcgg cgccaccaac
     2641 ttctccctgc tgaagcaggc cggcgacgtg gaggagaacc ccggccccgt gagcaagggc
     2701 gaggcagtga tcaaggagtt catgcggttc aaggtgcaca tggagggctc catgaacggc
     2761 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2821 aagctgaagg tgaccaaggg tggccccctg cccttctcct gggacatcct gtcccctcag
     2881 ttcatgtacg gctccagggc cttcaccaag caccccgccg acatccccga ctactataag
     2941 cagtccttcc ccgagggctt caagtgggac cgcgtgatga acttcgagga cggcggcgcc
     3001 gtgaccgtga cccaggacac ctccctggag gacggcaccc tgatctacaa ggtgaagctc
     3061 cgcggcacca acttccctcc tgacggcccc gtaatgcaga agaagacaat gggctgggaa
     3121 gcgtccaccg agcggttgta ccccgaggac ggcgtgctga agggcgacat taagatggcc
     3181 ctgcgcctga aggacggcgg gcgctacctg gcggacttca agaccaccta caaggccaag
     3241 aagcccgtgc agatgcccgg cgcctacaac gtggaccgca agttggacat cacctcccac
     3301 aacgaggact acaccgtggt ggaacagtac gaacgctccg agggccgcca ctccaccggc
     3361 ggcatggacg agctgtacaa gtaaataatc aacctctgga ttacaaaatt tgtgaaagat
     3421 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     3481 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     3541 ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3601 ctcggctgtt gggcactgac aattccgtgg tgacccagct ttcttgtaca aagtgggaat
     3661 tcctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     3721 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     3781 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     3841 ggggcaggac agcaaggggg aggattggga agagaatagc aggcatgctg gggagggccg
     3901 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
     3961 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
     4021 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
     4081 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
     4141 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
     4201 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4261 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4321 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4381 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4441 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
     4501 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4561 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     4621 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     4681 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     4741 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     4801 tcatgataat aatggtttct tagacgtcct ggcccgtgtc tcaaaatctc tgatgttaca
     4861 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta
     4921 atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga ttaaattcca
     4981 acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg caatcaggtg
     5041 cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg aaacatggca
     5101 aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg ctgacggaat
     5161 ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca tggttactca
     5221 ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct gattcaggtg
     5281 aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt cctgtttgta
     5341 attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca cgaatgaata
     5401 acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct gttgaacaag
     5461 tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc actcatggtg
     5521 atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt attgatgttg
     5581 gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac tgcctcggtg
     5641 agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat aatcctgata
     5701 tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa ttggttaatt
     5761 ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc tcatgaccaa
     5821 aatcccttaa cgtgagttac gcgtgaagat cctttttgat aatctcatga ccaaaatccc
     5881 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5941 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6001 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6061 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6121 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6181 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6241 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6301 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6361 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6421 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6481 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6541 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
