LOCUS       521_MRo_MBP-JM3-        8444 bp    DNA     circular UNA 12-APR-2021
DEFINITION  modified 341_MRo_MBP-JM3-Trx-PreSc_EC, Nde I and Age I introduced.
ACCESSION   urn.local...64-dhoqa3s
VERSION     urn.local...64-dhoqa3s
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     intron          256..1347
                     /standard_name="LacI reg"
     promoter        1396..1425
                     /standard_name="lac prom"
     gene            1510..4155
                     /standard_name="AG465-2xPreScission"
     gene            <2686..>2760
                     /standard_name="AG465-2xPreScission"
     misc_feature    2707..3786
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CATCTGGAAGTTCTGTTCCAGGGGCCCATGGGGGCCGAGCTG
                     CTGTCGCAGCAGTGGGAGGCGGGCATGAGCCTGCTGATGGCCCTGGTGGTGCTGCTCA
                     TCGTGGCCGGCAACGTGCTGGTGATCGCGGCCATCGGGAGCACGCAGCGGCTGCAGAC
                     GCTCACCAACCTCTTCATCACCTCGCTGGCCTGCGCCGACCTGGTGGTGGGGCTGCTG
                     GTGGTGCCTTTCGGGGCCACGCTGGTGGTGCGGGGCACCTGGCTGTGGGGCTCCTTCC
                     TCTGCGAGCTCTGGACATCGCTGGACGTGCTTTGCGTGACGGCAAGCGTGGAGACCTT
                     GTGCGTCATCGCCATCGACCGCTACCTGGCCATCACCTCTCCATTCCGCTACCAGAGC
                     CTGATGACCAGGGCTCGGGCCAAGGTCATCATCTGCACCGTCTGGGCCATCTCCGCTC
                     TGGTCTCTTTCCTGCCCATCATGATGCACTGGTGGCGGGACGAGGACCCTCAGGCGCT
                     CAAGTGCTACCAGGACCCGGGCTGCTGCGAATTTGTCACCAACCGGGCTTACGCCATC
                     GCCTCGTCCATCATCTCCTTCTACATCCCCCTCCTCATCATGATCTTCGTGGCGCTGC
                     GGGTGTACCGGGAGGCCAAGGAGCAGATCAGGAAGATCGACCGCGCTAGCAAGAGGAA
                     GACGTCCCGTGTCATGCTGATGAGGGAACACAAAGCTCTGAAGACATTGGGTATCATC
                     ATGGGGGTGTTCACCCTCTGCTGGCTCCCTTTCTTCTTGGTGAACATTGTCAACGTCT
                     TCAACAGAGATCTGGTGCCGAAATGGCTCTTCGTTGCCTTCAACTGGTTGGGCTACGC
                     CAACTCTGCTATGAACCCCATCATCCTCTGCCGCAGCCCAGACTTCCGTAAGGCCTTC
                     AAGAGGCTGCTCGCCTTCCCCCGCAAAGCTGACAGGCGGCTGCTCGAGCTGGAAGTGC
                     TGTTTCAGGGCCCG"
     CDS             2716..2739
                     /standard_name="PreScission site"
     CDS             2767..2772
                     /standard_name="Nde I"
     CDS             3706..3711
                     /standard_name="Age I"
     terminator      4419..4576
                     /standard_name="rrnB term"
     terminator      4717..4744
                     /standard_name="rrnB_T2 term"
     promoter        4786..4814
                     /standard_name="amp prom"
     intron          4856..5716
                     /standard_name="amp"
     intron          complement(7942..8133)
                     /standard_name="ROP"
ORIGIN      
        1 gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
       61 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
      121 ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga
      181 aattccgaca ccatcgaatg gtgcaaaacc tttcgcggta tggcatgata gcgcccggaa
      241 gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt tatacgatgt cgcagagtat
      301 gccggtgtct cttatcagac cgtttcccgc gtggtgaacc aggccagcca cgtttctgcg
      361 aaaacgcggg aaaaagtgga agcggcgatg gcggagctga attacattcc caaccgcgtg
      421 gcacaacaac tggcgggcaa acagtcgttg ctgattggcg ttgccacctc cagtctggcc
      481 ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc gcgccgatca actgggtgcc
      541 agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac
      601 aatcttctcg cgcaacgcgt cagtgggctg atcattaact atccgctgga tgaccaggat
      661 gccattgctg tggaagctgc ctgcactaat gttccggcgt tatttcttga tgtctctgac
      721 cagacaccca tcaacagtat tattttctcc catgaagacg gtacgcgact gggcgtggag
      781 catctggtcg cattgggtca ccagcaaatc gcgctgttag cgggcccatt aagttctgtc
      841 tcggcgcgtc tgcgtctggc tggctggcat aaatatctca ctcgcaatca aattcagccg
      901 atagcggaac gggaaggcga ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg
      961 ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca acgatcagat ggcgctgggc
     1021 gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg cggatatctc ggtagtggga
     1081 tacgacgata ccgaagacag ctcagtggat atcccgccgt taaccaccat caaacaggat
     1141 tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc aactctctca gggccaggcg
     1201 gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc
     1261 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     1321 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     1381 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     1441 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaatttc tagataacga
     1501 gggcaaacca tgaaaataaa aacaggtgca cgcatcctcg cattatccgc attaacgacg
     1561 atgatgtttt ccgcctcggc tctcgccaaa atcgaagaag gtaaactggt aatctggatt
     1621 aacggcgata aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc
     1681 ggaattaaag tcaccgttga gcatccggat aaactggaag agaaattccc acaggttgcg
     1741 gcaactggcg atggccctga cattatcttc tgggcacacg accgctttgg tggctacgct
     1801 caatctggcc tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg
     1861 tttacctggg atgccgtacg ttacaacggc aagctgattg cttacccgat cgctgttgaa
     1921 gcgttatcgc tgatttataa caaagatctg ctgccgaacc cgccaaaaac ctgggaagag
     1981 atcccggcgc tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg
     2041 caagaaccgt acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat
     2101 gaaaacggca agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt
     2161 ctgaccttcc tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc
     2221 atcgcagaag ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca
     2281 tggtccaaca tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag
     2341 ggtcaaccat ccaaaccgtt cgttggcgtg ctgagcgcag gtattaacgc cgccagtccg
     2401 aacaaagagc tggcaaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa
     2461 gcggttaata aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg
     2521 gcgaaagatc cacgtattgc cgccaccatg gaaaacgccc agaaaggtga aatcatgccg
     2581 aacatcccgc agatgtccgc tttctggtat gccgtgcgta ctgcggtgat caacgccgcc
     2641 agcggtcgtc agactgtcga tgaagccctg aaagacgcgc agactggatc tggttctggt
     2701 tctggctcag ggtcactgga agttctgttc caggggcccg gtagcggaag tggatccggg
     2761 agcggacata tgggggccga gctgctgtcg cagcagtggg aggcgggcat gagcctgctg
     2821 atggccctgg tggtgctgct catcgtggcc ggcaacgtgc tggtgatcgc ggccatcggg
     2881 agcacgcagc ggctgcagac gctcaccaac ctcttcatca cctcgctggc ctgcgccgac
     2941 ctggtggtgg ggctgctggt ggtgcctttc ggggccacgc tggtggtgcg gggcacctgg
     3001 ctgtggggct ccttcctctg cgagctctgg acatcgctgg acgtgctttg cgtgacggca
     3061 agcgtggaga ccttgtgcgt catcgccatc gaccgctacc tggccatcac ctctccattc
     3121 cgctaccaga gcctgatgac cagggctcgg gccaaggtca tcatctgcac cgtctgggcc
     3181 atctccgctc tggtctcttt cctgcccatc atgatgcact ggtggcggga cgaggaccct
     3241 caggcgctca agtgctacca ggacccgggc tgctgcgaat ttgtcaccaa ccgggcttac
     3301 gccatcgcct cgtccatcat ctccttctac atccccctcc tcatcatgat cttcgtggcg
     3361 ctgcgggtgt accgggaggc caaggagcag atcaggaaga tcgaccgcgc tagcaagagg
     3421 aagacgtccc gtgtcatgct gatgagggaa cacaaagctc tgaagacatt gggtatcatc
     3481 atgggggtgt tcaccctctg ctggctccct ttcttcttgg tgaacattgt caacgtcttc
     3541 aacagagatc tggtgccgaa atggctcttc gttgccttca actggttggg ctacgccaac
     3601 tctgctatga accccatcat cctctgccgc agcccagact tccgtaaggc cttcaagagg
     3661 ctgctcgcct tcccccgcaa agctgacagg cggctgctcg agggcaccgg tagtggtagt
     3721 ggatctggta gtctggaagt gctgtttcag ggcccgggta gcggtagtgg ctccggctcc
     3781 gggagcgaat tcagcgataa aattattcac ctgactgacg acagttttga cacggatgta
     3841 ctcaaagcgg acggggcgat cctcgtcgat ttctgggcag agtggtgcgg tccgtgcaaa
     3901 atgatcgccc cgattctgga tgaaatcgct gacgaatatc agggcaaact gaccgttgca
     3961 aaactgaaca tcgatcaaaa ccctggcact gcgccgaaat atggcatccg tggtatcccg
     4021 actctgctgc tgttcaaaaa cggtgaagtg gcggcaacca aagtgggtgc actgtctaaa
     4081 ggtcagttga aagagttcct cgacgctaac ctggcgggta cccaccatca ccatcaccat
     4141 caccatcacc attaataatc tagaaagctt ggcactggcc gtcgttttac aacgtcgtga
     4201 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     4261 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     4321 tggcgaatgg cagcttggct gttttggcgg atgagataag attttcagcc tgatacagat
     4381 taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt
     4441 ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt
     4501 ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt
     4561 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga
     4621 caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag
     4681 gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc
     4741 tttttgcgtt tctacaaact ctttttgttt atttttctaa atacattcaa atatgtatcc
     4801 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     4861 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     4921 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     4981 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5041 acgttctcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     5101 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5161 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5221 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5281 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5341 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5401 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5461 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5521 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     5581 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     5641 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     5701 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttacc
     5761 ccggttgata atcagaaaag ccccaaaaac aggaagattg tataagcaaa tatttaaatt
     5821 gtaaacgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     5881 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagcc cgagatccgg
     5941 ccgaacaaac tccgggaggc agcgtgatgc ggcaacaatc acacggattt cccgtgaacg
     6001 gtctgaatga gcggattatt ttcagggaaa gtgagtgtgg tcagcgtgca ggtatatggg
     6061 ctatgatgtg cccggcgctt gaggctttct gcctcatgac gtgaaggtgg tttgttgccg
     6121 tgttgtgtgg cagaaagaag atagccccgt agtaagttaa ttttcattaa ccaccacgag
     6181 gcatccctat gtctagtcca catcaggata gcctcttacc gcgctttgcg caaggagaag
     6241 aaggccatga aactaccacg aagttccctt gtctggtgtg tgttgatcgt gtgtctcaca
     6301 ctgttgatat tcacttatct gacacgaaaa tcgctgtgcg agattcgtta cagagacgga
     6361 cacagggagg tggcggcttt catggcttac gaatccggta agtagcaacc tagaggcggg
     6421 cgcaggcccg ccttttcagg actgatgctg gtctgactac tgaagcgcct ttataaaggg
     6481 gctgctggtt cgccggtagc ccctttctcc ttgctgatgt tgtgggaatt cctgaatcgc
     6541 cccatcatcc agccagaaag tgagggagcc acggttgatg agagctttgt tgtaggtgga
     6601 ccagttggtg attttgaact tttgctttgc cacggaacgg tctgcgttgt cgggaagatg
     6661 tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     6721 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     6781 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     6841 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt aaaaggatct
     6901 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6961 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7021 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7081 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7141 atactgtcct tctagtgtag cggtagttag gccaccactt caagaactct gtagcaccgc
     7201 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7261 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7321 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7381 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7441 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7501 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7561 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7621 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7681 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7741 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     7801 atctgtgcgg tatttcacac cgcatagggt catggctgcg ccccgacacc cgccaacacc
     7861 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     7921 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     7981 gctgcggtaa agctcatcag cgtggtcgtg cagcgattca cagatgtctg cctgttcatc
     8041 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     8101 catgttaagg gcggtttttt cctgtttggt cacttgatgc ctccgtgtaa gggggaattt
     8161 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     8221 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     8281 cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta
     8341 ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag
     8401 ggcgctgact tccgcgtttc cagactttac gaaacacgga aacc
//
