LOCUS       JON-0371_-_pMH180_pDES 13128 bp ds-DNA     circular     20-MAY-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     restriction ... 19..24
                     /label="Acc16I, FspI, NsbI"
     restriction ... 39..44
                     /label="PvuI, Ple19I"
     restriction ... 117..123
                     /label="PasI"
     primer_bind     143..159
                     /label="M13 fwd"
     enhancer        312..690
                     /label="CMV enhancer"
     restriction ... 326..331
                     /label="SpeI, AhlI, BcuI"
     restriction ... 559..564
                     /label="NdeI, FauNDI"
     restriction ... 664..669
                     /label="BsaAI, BstBAI, Ppu21I"
     restriction ... 664..669
                     /label="SnaBI, BstSNI, Eco105I"
     promoter        691..894
                     /label="CMV promoter"
     restriction ... 714..725
                     /label="CspCI"
     restriction ... 890..895
                     /label="Ecl136II, Eco53kI, EcoICRI"
     restriction ... 890..895
                     /label="BanII, Eco24I, EcoT38I, FriOI"
     restriction ... 890..895
                     /label="SacI, Psp124BI, SstI"
     CDS             925..1014
                     /label="Translation 925-1014"
                     /translation="MDYKDDDDKLAAANSSIDLISTSLYKKAGS"
     CDS             928..951
                     /label="FLAG"
     restriction ... 949..954
                     /label="HindIII"
     restriction ... 955..962
                     /label="NotI, CciNI"
     restriction ... 956..961
                     /label="BseX3I, BstZI, EagI, EclXI, Eco52I"
     restriction ... 963..968
                     /label="EcoRI"
     restriction ... 969..974
                     /label="ClaI, Bsa29I, BseCI, BshVI, BspDI, Bsu15I, BsuTUI"
     restriction ... 975..980
                     /label="BglII"
     restriction ... 981..986
                     /label="EcoRV, Eco32I"
     protein_bind    988..1005
                     /label="attR1"
     misc_feature    1015..1017
                     /label="start"
     misc_feature    1015..9414
                     /label="UBR5"
     CDS             1015..9414
                     /label="Translation 1015-9414"
                     /translation="MTSIHFVVHPLPGTEDQLNDRLREVSEKLNKYNLNSHPPLNVLEQATIKQCVVGPNHAAFLLEDGRVCRIGFSVQPDRLELGKPDNNDGSKLNSNSGAGRTSRPGRTSDSPWFLSGSETLGRLAGNTLGSRWSSGVGGSGGGSSGRSSAGARDSRRQTRVIRTGRDRGSGLLGSQPQPVIPASVIPEELISQAQVVLQGKSRSVIIRELQRTNLDVNLAVNNLLSRDDEDGDDGDDTASESYLPGEDLMSLLDADIHSAHPSVIIDADAMFSEDISYFGYPSFRRSSLSRLGSSRVLLLPLERDSELLRERESVLRLRERRWLDGASFDNERGSTSKEGEPNLDKKNTPVQSPVSLGEDLQWWPDKDGTKFICIGALYSELLAVSSKGELYQWKWSESEPYRNAQNPSLHHPRATFLGLTNEKIVLLSANSIRATVATENNKVATWVDETLSSVASKLEHTAQTYSELQGERIVSLHCCALYTCAQLENSLYWWGVVPFSQRKKMLEKARAKNKKPKSSAGISSMPNITVGTQVCLRNNPLYHAGAVAFSISAGIPKVGVLMESVWNMNDSCRFQLRSPESLKNMEKASKTTEAKPESKQEPVKTEMGPPPSPASTCSDASSIASSASMPYKRRRSTPAPKEEEKVNEEQWSLREVVFVEDVKNVPVGKVLKVDGAYVAVKFPGTSSNTNCQNSSGPDADPSSLLQDCRLLRIDELQVVKTGGTPKVPDCFQRTPKKLCIPEKTEILAVNVDSKGVHAVLKTGNWVRYCIFDLATGKAEQENNFPTSSIAFLGQNERNVAIFTAGQESPIILRDGNGTIYPMAKDCMGGIRDPDWLDLPPISSLGMGVHSLINLPANSTIKKKAAVIIMAVEKQTLMQHILRCDYEACRQYLMNLEQAVVLEQNLQMLQTFISHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSSSRSLRLREMMRRSLRAAGLGRHEAGASSSDHQDPVSPPIAPPSWVPDPPAMDPDGDIDFILAPAVGSLTTAATGTGQGPSTSTIPGPSTEPSVVESKDRKANAHFILKLLCDSVVLQPYLRELLSAKDARGMTPFMSAVSGRAYPAAITILETAQKIAKAEISSSEKEEDVFMGMVCPSGTNPDDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTATNLVTLPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKTASPEDSDMPDHDLEPPRFAQLALERVLQDWNALKSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGTIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAIAVTMRFLRSVARVFVILSVEMASSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQSSSSSQSQSSYIIRNPQQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENAEAEGQHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDIEQETFMLDEPLERTTNSSHANGAAQAPRSMQWAVRNTQHQRAASTAPSSTSTPAASSAGLIYIDPSNLRRSGTISTSAAAAAAALEASNASSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLTYQDAVNLQNYVEEKLIPTWNWMVSIMDSTEAQLRYGSALASAGDPGHPNHPLHASQNSARRERMTAREEASLRTLEGRRRATLLSARQGMMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGIDNEDSEHENDDDTNQSATLNDKDDDSLPAETGQNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSSASSGKCLMEVTVDRNCLEVLPTKMSYAANLKNVMNMQNRQKKEGEEQPVLPEETESSKPGPSAHDLAAQLKSSLLAEIGLTESEGPPLTSFRPQCSFMGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLSLEVDRDRDLLIQQTMRQLNNHFGRRCATTPMAVHRVKVTFKDEPGEGSGVARSFYTAIAQAFLSNEKLPNLECIQNANKGTHTSLMQRLRNRGERDREREREREMRRSSGLRAGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPEPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELIIAHGRENGADSILDLGLVDSSEKVQQENRKRHGSSRSVVDMDLDDTDDGDDNAPLFYQPGKRGFYTPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLNRHVIKVLLGRKVNWHDFAFFDPVMYESLRQLILASQSSDADAVFSAMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLVVAEQPLHAMRKGLLDVLPKNSLEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV*"
     misc_feature    7660..7935
                     /label="HECT_insertion"
     misc_feature    7993..8118
                     /label="PolyR Tract"
     misc_feature    8184..8409
                     /label="MLLE"
     misc_feature    9412..9414
                     /label="stop"
     CDS             9415..9425
                     /label="Translation 9415-9425"
                     /translation="DPA"
     CDS             9424..9524
                     /label="Translation 9424-9524"
                     /translation="FLYKVVDIGTSRL*RIPGGIPVTPPQCLSWPWK"
     protein_bind    complement(9426..9440)
                     /label="attR2"
     restriction ... 9442..9447
                     /label="EcoRV, Eco32I"
     restriction ... 9448..9453
                     /label="KpnI"
     restriction ... 9448..9453
                     /label="Acc65I, Asp718I"
     restriction ... 9455..9460
                     /label="SalI"
     restriction ... 9461..9466
                     /label="XbaI"
     restriction ... 9471..9476
                     /label="Cfr9I, TspMI, XmaI"
     restriction ... 9471..9476
                     /label="SmaI"
     restriction ... 9527..9536
                     /label="OliI, AleI"
     restriction ... 9538..9549
                     /label="CspCI"
     restriction ... 9582..9592
                     /label="Eam1105I, AhdI, BmeRI, DriI"
     restriction ... 9648..9653
                     /label="BbsI, BpiI, BstV2I"
     restriction ... 9661..9667
                     /label="EcoO109I"
     restriction ... 9695..9700
                     /label="BsgI"
     restriction ... 9795..9801
                     /label="BlpI, Bpu1102I, Bsp1720I"
     restriction ... 9822..9827
                     /label="Esp3I, BsmBI"
     restriction ... 9896..9907
                     /label="BstXI"
     restriction ... 9920..9932
                     /label="AloI"
     restriction ... 9999..10004
                     /label="BspMI, Acc36I, BfuAI, BveI"
     restriction ... 10011..10021
                     /label="PflMI, AccB7I, Van91I"
     restriction ... 10014..10019
                     /label="AgeI, AsiGI, BshTI, CspAI, PinAI"
     promoter        10118..10448
                     /label="SV40 promoter"
     restriction ... 10171..10181
                     /label="BstAPI"
     restriction ... 10199..10205
                     /label="SexAI, CsiI, MabI"
     restriction ... 10244..10254
                     /label="BstAPI"
     rep_origin      10299..10434
                     /label="SV40 ori"
     restriction ... 10377..10387
                     /label="BplI"
     restriction ... 10379..10391
                     /label="SfiI"
     restriction ... 10414..10419
                     /label="BseRI"
     restriction ... 10430..10435
                     /label="StuI, Eco147I, PceI, SseBI"
     restriction ... 10433..10438
                     /label="AvrII, AspA2I, BlnI, XmaJI"
     restriction ... 10452..10457
                     /label="BseRI"
     restriction ... 10454..10461
                     /label="PspXI"
     restriction ... 10454..10461
                     /label="AbsI"
     restriction ... 10455..10460
                     /label="XhoI, PaeR7I, Sfr274I, SlaI"
     promoter        complement(10487..10505)
                     /label="T7 promoter"
     primer_bind     complement(10519..10535)
                     /label="M13 rev"
     protein_bind    10543..10559
                     /label="lac operator"
     promoter        complement(10567..10573)
                     /label="lac promoter"
     promoter        complement(10574..10591)
                     /label="lac promoter"
     promoter        complement(10592..10597)
                     /label="lac promoter"
     protein_bind    10612..10633
                     /label="CAP binding site"
     restriction ... 10737..10743
                     /label="SapI, BspQI, LguI, PciSI"
     restriction ... 10860..10865
                     /label="PciI, PscI"
     restriction ... 10860..10865
                     /label="AflIII"
     rep_origin      complement(10921..11509)
                     /label="ori"
     restriction ... 10962..10973
                     /label="DrdI, AasI, DseDI"
     restriction ... 11033..11038
                     /label="BauI, BssSI, Bst2BI"
     restriction ... 11387..11392
                     /label="Eco57I, AcuI"
     restriction ... 11580..11585
                     /label="BspHI, CciI, PagI"
     CDS             complement(11680..12471)
                     /label="AmpR"
     restriction ... 11748..11758
                     /label="Eam1105I, AhdI, BmeRI, DriI"
     restriction ... 11973..11978
                     /label="Acc16I, FspI, NsbI"
     restriction ... 12120..12125
                     /label="PvuI, Ple19I"
     restriction ... 12231..12236
                     /label="ScaI, BmcAI, ZrmI"
     restriction ... 12348..12357
                     /label="XmnI, Asp700I, MroXI, PdmI"
     restriction ... 12417..12422
                     /label="BauI, BssSI, Bst2BI"
     restriction ... 12435..12440
                     /label="Eco57I, AcuI"
     CDS             complement(12472..12540)
                     /label="AmpR"
     promoter        complement(12541..12645)
                     /label="AmpR promoter"
     restriction ... 12588..12593
                     /label="BspHI, CciI, PagI"
     rep_origin      12672..13128
                     /label="f1 ori"
     restriction ... 12797..12802
                     /label="NaeI, PdiI"
     restriction ... 12797..12802
                     /label="KroI, MroNI, NgoMIV"
     restriction ... 12900..12905
                     /label="BsaAI, BstBAI, Ppu21I"
     restriction ... 12900..12908
                     /label="DraIII, AdeI"
     restriction ... 12943..12954
                     /label="DrdI, AasI, DseDI"
     restriction ... 12949..12961
                     /label="AloI"
     restriction ... 13028..13033
                     /label="PsiI, AanI"
ORIGIN
        1 Cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccca
      121 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgat ctaatcaata
      181 ttggccatta gccatattat tcattggtta tatagcataa atcaatattg gctattggcc
      241 attgcatacg ttgtatccat atcataatat gtacatttat attggctcat gtccaacatt
      301 accgccatgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
      361 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      421 cgaccgccca gcgacccccg cccgttgacg tcaatagtga cgtatgttcc catagtaacg
      481 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      541 gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa
      601 tggcccgcct agcattatgc ccagtacatg accttacggg agtttcctac ttggcagtac
      661 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg
      721 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      781 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg
      841 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtcT ATATAAGCAG AGCTCgttta
      901 gtgaaccgtc agaattgatc taccatggac tacaaagacg atgacgacaa gcttgcggcc
      961 gcgaattcat cgatagatct gatatcaACA AGTTTGTACA AAAAAgcagg ctccatgacg
     1021 tccatccatt tcgtggttca cccgctgccg ggcaccgagg accagctcaa tgacaggtta
     1081 cgagaagttt ctgagaagct gaacaaatat aatttaaaca gccacccccc tttgaatgta
     1141 ttggaacagg ctactattaa acagtgtgtg gtgggaccaa atcatgctgc ctttcttctt
     1201 gaggatggta gagtttgcag gattggtttt tcagtacagc cagacagatt ggaattgggt
     1261 aaacctgata ataatgatgg gtcaaagttg aacagcaact cgggggcagg gaggacgtca
     1321 aggcctggta ggacaagcga ctctccatgg tttctctcag gttctgagac tctaggcagg
     1381 ctggcaggca acaccttagg aagccgctgg agttctggag tgggtggaag tggtggagga
     1441 tcctctggta ggtcatcagc tggagctcga gattcccgcc ggcagactcg agttattcgg
     1501 acaggacggg atcgagggtc tgggcttttg ggcagtcagc cccagccagt tattccagca
     1561 tctgtcattc cagaggagct gatttcacag gcccaagttg ttttacaagg caaatccaga
     1621 agtgtcatta ttcgagaact tcagagaaca aatcttgatg tgaaccttgc tgtaaataat
     1681 ttacttagcc gggatgatga agatggagat gatggggatg atacagccag cgaatcttat
     1741 ttgcctggag aggatcttat gtctctcctt gatgccgaca ttcattctgc ccacccaagt
     1801 gtcattattg atgcagatgc catgttttct gaagacatta gctattttgg ttacccttct
     1861 tttcgtcgtt catcactttc caggctaggc tcatctcgag ttctccttct tcccttagag
     1921 agagactctg agctgttgcg tgaacgtgaa tccgttttac gtttacgtga acgaaggtgg
     1981 cttgatggag cctcatttga taatgaaagg ggttctacca gcaaggaagg agagccaaac
     2041 ttggataaga agaatacacc tgttcaaagt ccagtatctc taggagaaga tttgcagtgg
     2101 tggcctgata aggatggaac aaaattcatc tgtattgggg ctctgtattc tgaacttctg
     2161 gctgtcagca gtaaaggaga actttatcag tggaaatgga gtgaatctga gccttacaga
     2221 aatgcccaga atccttcatt acatcatcca cgagcaacat ttttggggtt aaccaatgaa
     2281 aagatagtcc tcctgtctgc aaatagcata agagcaactg tagctacaga aaataacaag
     2341 gttgctacat gggtggatga aactttaagt tctgtggctt ctaaattaga gcacactgct
     2401 cagacttact ctgaacttca aggagagcgg atagtttctt tacattgctg tgccctttac
     2461 acctgcgctc agctggaaaa cagtttatat tggtggggtg tagttccttt tagtcaaagg
     2521 aagaaaatgt tagagaaagc tagagcaaaa aataaaaagc ctaaatccag tgctggtatt
     2581 tcttcaatgc cgaacatcac tgttggtacc caggtatgct tgagaaataa tcctctttat
     2641 catgctggag cagttgcatt ttcaattagt gctgggattc ctaaagttgg tgtcttaatg
     2701 gagtcagttt ggaatatgaa tgacagctgt agatttcaac ttagatctcc tgaaagcttg
     2761 aaaaacatgg aaaaagctag caaaactact gaagctaagc ctgaaagtaa gcaggagcca
     2821 gtgaaaacag aaatgggtcc tccaccatct ccagcatcca cgtgtagtga tgcatcctca
     2881 attgccagca gtgcatcaat gccatacaaa cgacgacggt caacccctgc accaaaagaa
     2941 gaggaaaagg tgaatgaaga gcagtggtct cttcgggaag tggtttttgt ggaagatgtc
     3001 aagaatgttc ctgttggcaa ggtgctaaaa gtagatggtg cctatgttgc tgtaaaattt
     3061 ccaggaacct ccagtaatac taactgtcag aacagctctg gtccagatgc tgacccttct
     3121 tctctcctgc aggattgtag gttacttaga attgatgaat tgcaggttgt caaaactggt
     3181 ggaacaccga aggttcccga ctgtttccaa aggactccta aaaagctttg tatacctgaa
     3241 aaaacagaaa tattagcagt gaatgtagat tccaaaggtg ttcatgctgt tctgaagact
     3301 ggaaattggg tgcgatactg tatctttgat cttgctacag gaaaagcaga acaggaaaat
     3361 aattttccta caagcagcat tgctttcctt ggtcagaatg agaggaatgt agccattttc
     3421 actgctggac aggaatctcc cattattctt cgagatggaa atggtaccat ctacccaatg
     3481 gccaaagatt gcatgggagg aataagggat cccgattggc tggatcttcc acctattagt
     3541 agtcttggaa tgggtgtgca ttctttaata aatcttcctg ccaattcaac aatcaaaaag
     3601 aaagctgctg ttatcatcat ggctgtagag aaacaaacct taatgcaaca cattctgcgc
     3661 tgtgactatg aggcctgtcg acaatatcta atgaatcttg agcaagcggt tgttttagag
     3721 cagaatctac agatgctgca gacattcatc agccacagat gtgatggaaa tcgaaatatt
     3781 ttgcatgctt gtgtatcagt ttgctttcca accagcaata aagaaactaa agaagaagag
     3841 gaagcggagc gttctgaaag aaatacattt gcagaaaggc tttctgctgt tgaggccatt
     3901 gcaaatgcaa tatcagttgt ttcaagtaat ggcccaggta atcgggctgg atcatcaagt
     3961 agccgaagtt tgagattacg ggaaatgatg agacgttcgt tgagagcagc tggtttgggt
     4021 agacatgaag ctggagcttc atccagtgac caccaggatc cagtttcacc ccccatagct
     4081 ccccctagtt gggttcctga ccctcctgcg atggatcctg atggtgacat tgattttatc
     4141 ctggcccccg ctgtgggatc tcttaccaca gcagcaaccg gtactggtca aggaccaagc
     4201 acctccacta ttccaggtcc ttccacagag ccatctgtag tagaatccaa ggatcgaaag
     4261 gcgaatgctc attttatatt gaaattgtta tgtgacagtg tggttctcca gccctatcta
     4321 cgagaacttc tttctgccaa ggatgcaaga gggatgaccc catttatgtc agctgtaagt
     4381 ggccgagctt atcctgctgc aattaccatc ttagaaactg ctcagaaaat tgcaaaagct
     4441 gaaatatcct caagtgaaaa agaggaagat gtattcatgg gaatggtttg cccatcaggt
     4501 accaaccctg atgactctcc tttatatgtt ttatgttgta atgacacttg cagttttaca
     4561 tggactggag cagagcacat taaccaggat atttttgagt gtcgaacttg tggcttgctg
     4621 gagtcactgt gttgttgtac ggaatgtgca agggtttgtc ataaaggtca tgattgcaaa
     4681 ctcaaacgga catcaccaac agcctactgt gattgttggg agaaatgtaa atgtaaaact
     4741 cttattgctg gacagaaatc tgctcgtctt gatctacttt atcgcctgct cactgctact
     4801 aatctggtta ctctgccaaa cagcagggga gagcacctct tactattctt agtacagaca
     4861 gtcgcaaggc agacggtgga gcattgtcaa tacaggccac ctcgaatcag ggaagatcgt
     4921 aaccgaaaaa cagccagtcc tgaagattca gatatgccag atcatgattt agagcctcca
     4981 agatttgccc agcttgcatt ggagcgtgtt ctacaggact ggaatgcctt gaaatctatg
     5041 attatgtttg ggtcgcagga gaataaagac cctcttagtg ccagcagtag aataggccat
     5101 cttttgccag aagagcaagt atacctcaat cagcaaagtg gcacaattcg gctggactgt
     5161 ttcactcatt gccttatagt taagtgtaca gcagatattt tgcttttaga tactctacta
     5221 ggtacactag tgaaagaact ccaaaacaaa tatacacctg gacgtagaga agaagctatt
     5281 gctgtgacaa tgaggtttct acgttcagtg gcaagagttt ttgttattct gagtgtggaa
     5341 atggcttcat ccaaaaagaa aaacaacttt attccacagc caattggaaa atgcaagcgt
     5401 gtattccaag cattgctacc ttacgctgtg gaagaattgt gcaacgtagc agagtcactg
     5461 attgttcctg tcagaatggg gattgctcgt ccaactgcac catttaccct ggctagtact
     5521 agcatagatg ccatgcaggg cagtgaagaa ttattttcag tggaaccact accaccacga
     5581 ccatcatctg atcagtctag cagctccagt cagtctcagt catcctacat catcaggaat
     5641 ccacagcaga ggcgcatcag ccagtcacag cccgttcggg gcagagatga agaacaggat
     5701 gatattgttt cagcagatgt ggaagaggtt gaggtggtgg agggtgtggc tggagaagag
     5761 gatcatcatg atgaacagga agaacacggg gaagaaaatg ctgaggcaga gggacaacat
     5821 gatgagcatg atgaagacgg gagtgatatg gagctggact tgttagcagc agctgaaaca
     5881 gaaagtgata gtgaaagtaa ccacagcaac caagataatg ctagtgggcg cagaagcgtt
     5941 gtcactgcag caactgctgg ttcagaagca ggagcaagca gtgttcctgc cttcttttct
     6001 gaagatgatt ctcaatcgaa tgactcaagt gattctgata gcagtagtag tcagagtgac
     6061 gacatagaac aggagacctt tatgcttgat gagccattag aaagaaccac aaatagctcc
     6121 catgccaatg gtgctgccca agctccccgt tcaatgcagt gggctgtccg caacacccag
     6181 catcagcgag cagccagtac agccccttcc agtacatcta caccagcagc aagttcagcg
     6241 ggtttgattt atattgatcc ttcaaactta cgccggagtg gtaccatcag tacaagtgct
     6301 gcagctgcag cagctgcttt ggaagctagc aacgccagca gttacctaac atctgcaagc
     6361 agtttagcca gggcttacag cattgtcatt agacaaatct cggacttgat gggccttatt
     6421 cctaagtata atcacctagt atactctcag attccagcag ctgtgaaatt gacttaccaa
     6481 gatgcagtaa acttacagaa ctatgtagaa gaaaagctta ttcccacttg gaactggatg
     6541 gtcagtatta tggattctac tgaagctcaa ttacgttatg gttctgcatt agcatctgct
     6601 ggtgatcctg gacatccaaa tcatcctctt cacgcttctc agaattcagc gagaagagag
     6661 aggatgactg cgcgagaaga agctagctta cgaacacttg aaggcagacg acgtgccacc
     6721 ttgcttagcg cccgtcaagg aatgatgtct gcacgaggag acttcctaaa ttatgctctg
     6781 tctctaatgc ggtctcataa tgatgagcat tctgatgttc ttccagtttt ggatgtttgc
     6841 tcattgaagc atgtggcata tgtttttcaa gcacttatat actggattaa ggcaatgaat
     6901 cagcagacaa cattggatac acctcaacta gaacgcaaaa ggacgcgaga actcttggaa
     6961 ctgggtattg ataatgaaga ttcagaacat gaaaatgatg atgacaccaa tcaaagtgct
     7021 actttgaatg ataaggatga tgactctctt cctgcagaaa ctggccaaaa ccatccattt
     7081 ttccgacgtt cagactccat gacattcctt gggtgtatac ccccaaatcc atttgaagtg
     7141 cctctggctg aagccatccc cttggctgat cagccacatc tgttgcagcc aaatgctaga
     7201 aaggaggatc tttttggccg tccaagtcag ggtctttatt cttcatctgc cagtagtggg
     7261 aaatgtttaa tggaggttac agtggataga aactgcctag aggttcttcc aacaaaaatg
     7321 tcttatgctg ccaatctgaa aaatgtaatg aacatgcaaa accggcaaaa aaaagaaggg
     7381 gaagaacagc ccgtgctgcc agaagaaact gagagttcaa aaccagggcc atctgctcat
     7441 gatcttgctg cacaattaaa aagtagctta ctagcagaaa taggacttac tgaaagtgaa
     7501 gggccacctc tcacatcttt caggccacag tgtagcttta tgggaatggt tatttcccat
     7561 gatatgctgc taggacgttg gcgcctttct ttagaactgt tcggcagggt attcatggaa
     7621 gatgttggag cagaacctgg atcaatccta actgaattgg gtggttttga ggtaaaagaa
     7681 tcaaaattcc gcagagaaat ggaaaaactg agaaaccagc agtcaagaga tttgtcacta
     7741 gaggttgatc gggatcgaga tcttctcatt cagcagacta tgaggcagct taacaatcac
     7801 tttggtcgaa gatgtgctac tacaccaatg gctgtacaca gagtaaaagt cacatttaag
     7861 gatgagccag gagagggcag tggtgtagca cgaagttttt atacagccat tgcacaagca
     7921 tttttatcaa atgaaaaatt gccaaatcta gagtgtatcc aaaatgccaa caaaggcacc
     7981 cacacaagtt taatgcagag attaaggaac cgaggagaga gagaccggga aagggagaga
     8041 gaaagggaaa tgaggaggag tagtggtttg cgagcaggtt ctcggaggga ccgggataga
     8101 gactttagaa gacagctttc catcgacact aggcccttta gaccagcctc tgaagggaat
     8161 cctagcgatg atcctgagcc tttgccagca catcggcagg cacttggaga gaggctttat
     8221 cctcgtgtac aagcaatgca accagcattt gcaagtaaaa tcactggcat gttgttggaa
     8281 ttatccccag ctcagctgct tctccttcta gcaagtgagg attctctgag agcaagagtg
     8341 gatgaggcca tggaactcat tattgcacat ggacgggaaa atggagctga tagtatcctg
     8401 gatcttggat tagtagactc ctcagaaaag gtacagcagg aaaaccgaaa gcgccatggc
     8461 tctagtcgaa gtgtagtaga tatggattta gatgatacag atgatggtga tgacaatgcc
     8521 cctttgtttt accaacctgg gaaaagagga ttttatactc caaggcctgg caagaacaca
     8581 gaagcaaggt tgaattgttt cagaaacatt ggcaggattc ttggactatg tctgttacag
     8641 aatgaactct gtcctatcac attgaataga catgtaatta aagtattgct tggtagaaaa
     8701 gtcaattggc atgattttgc tttttttgat cctgtaatgt atgagagttt gcggcaacta
     8761 atcctcgcgt ctcagagttc agatgctgat gctgttttct cagcaatgga tttggcattt
     8821 gcaattgacc tgtgtaaaga agaaggtgga ggacaggttg aactcattcc taatggtgta
     8881 aatataccag tcactccaca gaatgtatat gagtatgtgc ggaaatacgc agaacacaga
     8941 atgttggtag ttgcagaaca gcccttacat gcaatgagga aaggtctact agatgtgctt
     9001 ccaaaaaatt cattagaaga tttaacggca gaagatttta ggcttttggt aaatggctgc
     9061 ggtgaagtca atgtgcaaat gctgatcagt tttacctctt tcaatgatga atcaggagaa
     9121 aatgctgaga agcttctgca gttcaagcgt tggttctggt caatagtaga gaagatgagc
     9181 atgacagaac gacaagatct tgtttacttt tggacatcaa gcccatcact gccagccagt
     9241 gaagaaggat tccagcctat gccctcaatc acaataagac caccagatga ccaacatctt
     9301 cctactgcaa atacttgcat ttctcgactt tacgtcccac tctattcctc taaacagatt
     9361 ctcaaacaga aattgttact cgccattaag accaagaatt ttggttttgt gtaggaccca
     9421 gctttCTTGT ACAAAGTGGT tgatatcggt accagtcgac tctagaggat cccgggtggc
     9481 atccctgtga cccctcccca gtgcctctcc tggccctgga agttgccact ccagtgccca
     9541 ccagccttgt cctaataaaa ttaagttgca tcattttgtc tgactaggtg tccttctata
     9601 atattatggg gtggaggggg ggtggtatgg agcaaggggc aagttgggaa gacaacctgt
     9661 agggcctgcg gggtctattg ggaaccaagc tggagtgcag ggcacaatct tggctcactg
     9721 caatctccgc tctgggttca agcgattctc ctgcctcacc tccgagttgt gggattccag
     9781 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     9841 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     9901 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttaaaataa
     9961 ctataccagc aggaggacgt ccagacacag cataggctac ctgccatggc ccaaccggtg
    10021 ggacatttga gttgcttgct tggcactgtc ctctcatgcg ttgggtccac tcagtagatg
    10081 cctgttgaat tgggtacgcg gccagcttct gtggaatgtg tgtcagttag ggtgtggaaa
    10141 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    10201 caggtgtgga aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
    10261 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
    10321 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    10381 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    10441 tttgcaaaaa gctcctcgag gaactgaaaa accagaaagt taattcccta tagtgagtcg
    10501 tattaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    10561 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    10621 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    10681 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    10741 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10801 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10861 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10921 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10981 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    11041 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    11101 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    11161 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    11221 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11281 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    11341 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
    11401 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    11461 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    11521 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    11581 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    11641 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    11701 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    11761 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    11821 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    11881 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    11941 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    12001 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    12061 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    12121 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    12181 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    12241 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    12301 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    12361 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    12421 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    12481 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    12541 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    12601 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    12661 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
    12721 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
    12781 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg
    12841 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
    12901 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
    12961 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
    13021 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
    13081 acaaaaattt aacgcgaatt ttaacaaaat attaaacgtt tacaattt
//