LOCUS       Kozak-atpG-pLVX-TetOne 10125 bp ds-DNA     circular     13-MAR-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          5686..5705
                     /label="pLVXsequencing.FWD2"
                     /note="sequence: ATGTCTGGATCTCAAATCCC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          5857..5877
                     /label="pLVX-Gibson-F"
                     /note="sequence: aggggaggtggtctggatcTC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(5872..5928)
                     /label="Translation 5928-5872"
                     /translation="GGSGGSGGSMDYKDDDDK*"
     misc_feature    complement(5875..5928)
                     /label="FLAG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(5929..6789)
                     /label="atpG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(5929..6789)
                     /label="Translation 6789-5929"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRPYAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAEMKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAYDEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDTLLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQELTEIVSGAAAV"
     misc_feature    complement(6790..6795)
                     /label="Kozak"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(6795..6827)
                     /label="pLVX-Gibson-R"
                     /note="sequence: ttccgtaccacttcctaccctcgtaaagaattc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(7172..7189)
                     /label="pLVXsequencing.REV2"
                     /note="sequence: ACCCCGTGGAATTATCAC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    7185..7695
                     /label="hPGK"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    7185..8460
                     /label="hPGK-promoted TET3G"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    7714..8460
                     /label="TET3G"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             7714..8460
                     /label="TET-3G protein"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTDSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLPG*"
ORIGIN
        1 ggtttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
       61 tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag
      121 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa
      181 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt
      241 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca
      301 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga
      361 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg
      421 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct
      481 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct
      541 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
      601 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
      661 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
      721 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
      781 tatcagagag tgagaggcct tgacattgct agcgttttac cgtcgacctc tagctagagc
      841 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
      901 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      961 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     1021 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     1081 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1141 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1201 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1261 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1321 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1381 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1441 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1501 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1561 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1621 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1681 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1741 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt
     1801 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     1981 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     2041 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     2101 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     2161 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     2221 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     2281 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     2341 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     2401 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     2461 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     2521 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     2581 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     2641 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     2701 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     2761 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     2821 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     2881 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     2941 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     3001 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
     3061 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3121 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
     3181 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctagtggt
     3241 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     3301 gttaaatatg tactacaaac ttagtagttt ttaaagaaat tgtatttgtt aaatatgtac
     3361 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
     3421 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     3481 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     3541 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     3601 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     3661 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     3721 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     3781 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     3841 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     3901 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     3961 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     4021 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     4081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     4141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     4201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     4261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     4321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     4381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     4441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     4501 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     4561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     4621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     4681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     4741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     4801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     4861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     4921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     4981 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     5041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     5101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     5161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     5221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     5281 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     5341 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     5401 cctttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     5461 tagcaacaga catacaaact aaagaactac aaaaacaaat tacaaaaatt caaaattttc
     5521 gggtttatta cagggacagc agagatccag tttatcgact taacttgttt attgcagctt
     5581 ataatggtta caaataaggc aatagcatca caaatttcac aaataaggca tttttttcac
     5641 tgcattctag ttttggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctcaa
     5701 atccctcgga agctgcgcct gtcttaggtt ggagtgatac atttttatca cttttacccg
     5761 tctttggatt aggcagtagc tctgacggcc ctcctgtctt aggttagtga aaaatgtcac
     5821 tctcttaccc gtcattggct gtccagctta gctcgcaggg gaggtggtct gtcacttatc
     5881 atcatcgtct ttgtaatcca tggatcctcc gcttccgcca cttccaccaa cagcggcagc
     5941 gccagacacg atctcggtca gttcttgggt gatactggcc tgccgggcct tgttgtacac
     6001 cagctgcagt tctttgatca ggctgccgcc gttatcggtg gcagccttca tagccaccat
     6061 tctagcggcc tgttcgctgg ccaggttttc cacaacgccc tggtacacct ggctttccac
     6121 gtatctccgc agcagggtat ccagcagtgc cttaggatcg ggctcgtaca ggtagtccca
     6181 gctcttgtgc ttcaggtcat cgtcgtcgct ggcaggcaga ggcaacagct ggctgatggt
     6241 tggcacctgg ctcatggtgt tgatgaactt gttagacacg atgtacagct tatccagccg
     6301 gccttcatcg taggcctgca gcatgacttt cacggggccg atcagctcag acagagatgg
     6361 attgtcgccc atgcctgtca cttgggccac cacgtttccg ccaacgctgt tgaagaagct
     6421 gacgccctta gagccgatca tggccaggtc gcactgcacc cctttgtcgg tccaggtctt
     6481 catctcggcc agcagtttct tgaacagatt gatgttcagg ccgccacaca ggcctctatc
     6541 tgtagacacc accaggtagc ccacgcgctt cacgtcccga tcttccaggt aggggtgctt
     6601 gtactcgagg ttgccgtggg ccagatggcc aatcactttc cgcattgtct cggcgtatgg
     6661 tctggaggcg gccatcctat cctgggactt tctcatcttg ctggcggcca ccatttccat
     6721 ggccttggtg attttctggg tgttctgcac gctggcgatc ttggatctGA TCTCTTTGGC
     6781 GCCTGCCATG GTGGCAATTC TTTACGAGGG TAGGAAGtgg tacggaaagt tggtataaga
     6841 caaaagtgtt gtggaattga agtttactca aaaaatcagc actcttttat aggcgccctg
     6901 gtttacataa gcaaagctta tacgttctct atcactgata gggagtaaac tggatatacg
     6961 ttctctatca ctgataggga gtaaactgta gatacgttct ctatcactga tagggagtaa
     7021 actggtcata cgttctctat cactgatagg gagtaaactc cttatacgtt ctctatcact
     7081 gatagggagt aaagtctgca tacgttctct atcactgata gggagtaaac tcttcatacg
     7141 ttctctatca ctgataggga gtaaactcga ggtgataatt ccacggggtt ggggttgcgc
     7201 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     7261 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     7321 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     7381 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     7441 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     7501 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     7561 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     7621 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     7681 gacctctctc cccaggggga tcatcgaatt accatgtcta gactggacaa gagcaaagtc
     7741 ataaactctg ctctggaatt actcaatgga gtcggtatcg aaggcctgac gacaaggaaa
     7801 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     7861 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca ctcctgcccc
     7921 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct
     7981 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     8041 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     8101 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     8161 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     8221 ctgaaacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     8281 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc
     8341 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     8401 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
     8461 acgcgcgaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     8521 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     8581 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     8641 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8701 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8761 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa acgcgaccat gaccgagtac
     8821 aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg caccctcgcc
     8881 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg ccacatcgag
     8941 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg
     9001 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg
     9061 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc
     9121 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc
     9181 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg
     9241 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg
     9301 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg
     9361 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaac gcgtctggaa
     9421 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     9481 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     9541 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     9601 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     9661 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     9721 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     9781 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     9841 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     9901 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     9961 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattaa
    10021 ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
    10081 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtg
//