LOCUS       HaloTag_PINK1_SPARK_v1. 7300 bp ds-DNA     circular     08-SEP-2025
DEFINITION  Express PKA-SPARK, an activity reporter of protein kinase A in
            mammalian cells..
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            addgene-plasmid-106920-sequence-205454.gbk
FEATURES             Location/Qualifiers
     primer_bind     complement(44..63)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="pRS vectors, use to sequence yeast selectable marker"
     enhancer        235..614
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="human cytomegalovirus (CMV) immediate early promoter"
     primer_bind     769..789
                     /label="CMV-F"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        863..881
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     863..882
                     /label="T7"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="T7 promoter, forward primer"
     regulatory      901..910
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /regulatory_class="other"
     primer          complement(904..963)
                     /label="SPARK-back"
                     /note="sequence: cagggtgctttctttctgaatgttataatcgctcagggtgctccctccaccgcccatggt"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             907..2856
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MGGGGSTLSDYNIQKESTLHLVLRGGLESGLRSGSGGGDIMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRTIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNVKAVDIGPGLNLLQEDNPDLIGSEIARWLSTLEISGGSGSAGGSAGGSAGGSAGGSAGGSAGGSAGGSRGEIAKSLKEIAKSLKEIAWSLKEIAKSLKGGTEGRGSLLTCGDVEENPGPKLATMHGNGRFLTLKPLPDSIIQESLEIQQGVNPFFIGRSEDCNCKIEDSRLSRVHCFIFKKRHAVGKSMYESPAQGLDDIWYCHTGTNVSYLNNNRMIQGTKFLLQDGDEIKIIWDKNNKFVIGFKVEINDTTGLFNEGLGMLQEQRVVLKQTAEEKDLVKKLGSGSAGGSAGGSAGGSAGGSAGGSAGGSAGGSRTLREIEELLRKIIEDSVRSVAELEDIEKWLKKI"
     misc_feature    925..978
                     /label="PINK1 Ubiquitin consensus sequence"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             925..978
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="TLSDYNIQKESTLHLVLR"
     misc_feature    931..957
                     /label="PSPARKrevAR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(933..971)
                     /label="quikPSPARKrev"
                     /note="sequence: accagaatcagggtgctttctttctgaatgttataatcg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          940..999
                     /label="SPARK-for"
                     /note="sequence: aacattcagaaagaaagcaccctgcatctggtgctgcgcggcggactcgagtccggactc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    958..971
                     /label="OR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          958..982
                     /label="quikPSPARKfor"
                     /note="sequence: accctgattctggtgctgcgcggcg"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    972..982
                     /label="PSPARKforAR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1001..1024
                     /label="ARback"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(1001..1041)
                     /label="Halofrontback"
                     /note="sequence: gccgatctcggccatgatatcgcctccaccgctgccagatc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1025..1041
                     /label="OR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1025..1062
                     /label="Halofrontfor"
                     /note="sequence: tcatggccgagatcggcaccggcttccccttcgacccc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1025..1890
                     /label="Piece B"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1027..1917
                     /label="HaloTag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1042..1062
                     /label="ARfor"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(1831..1890)
                     /label="Halobackrevfinal"
                     /note="sequence: gcgggcgatctcgctgccgatcaggtcggggttgtcctcctggagcaggttcaggccggg"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1861..1920
                     /label="Halobackforfinal"
                     /note="sequence: cccgacctgatcggcagcgagatcgcccgctggctgagcaccctcgagatcagcggcgga"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1867..1888
                     /label="ARback"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1889..1904
                     /label="OR"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1905..1925
                     /label="ARfor"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2017..2106
                     /label="HOTag3"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          2021..2047
                     /label="HaloTagfrickfor"
                     /note="sequence: aaatcgcaaagagtctgaaagaaattg"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             2113..2166
                     /label="T2A"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides."
                     /product="2A peptide from Thosea asigna virus capsid protein"
                     /translation="EGRGSLLTCGDVEENPGP"
     primer          2155..2214
                     /label="FHA2-front-for"
                     /note="sequence: aatcctggcccaaagcttgccaccatgcatggtaatggtaggtttttaactttaaaacca"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2155..2214)
                     /label="FHA2-front-back"
                     /note="sequence: tggttttaaagttaaaaacctaccattaccatgcatggtggcaagctttgggccaggatt"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2185..2658
                     /label="FHA2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2629..2688
                     /label="FHA2-end-for"
                     /note="sequence: gccgaagaaaaagatttggtgaaaaagttaggatccggcagtgctggtggtagtgctggt"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2629..2688)
                     /label="FHA2-end-back"
                     /note="sequence: accagcactaccaccagcactgccggatcctaactttttcaccaaatctttttcttcggc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2758..2856
                     /label="HOTag6"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     complement(2891..2908)
                     /label="BGH-rev"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse"
     polyA_signal    2897..3121
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3167..3595
                     /label="f1 ori"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
                     /direction="RIGHT"
     primer_bind     complement(3254..3273)
                     /label="F1ori-R"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="F1 origin, reverse primer"
     primer_bind     3464..3485
                     /label="F1ori-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="F1 origin, forward primer"
     primer_bind     complement(3604..3624)
                     /label="pBABE 3'"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3609..3938
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="SV40 enhancer and early promoter"
     rep_origin      3789..3924
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="SV40 origin of replication"
     primer_bind     3851..3870
                     /label="SV40pro-F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="SV40 promoter/origin, forward primer"
     CDS             4005..4799
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))"
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     primer_bind     complement(4059..4078)
                     /label="Neo-R"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4669..4688
                     /label="Neo-F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4973..5094
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5010..5029)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="SV40 polyA, reverse primer"
     primer_bind     5064..5083
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5143..5159)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="common sequencing primer, one of multiple similar variants"
     primer_bind     complement(5143..5159)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5156..5178)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="In lacZ gene"
     protein_bind    5167..5183
                     /label="lac operator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /bound_moiety="lac repressor encoded by lacI"
     promoter        complement(5191..5221)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5236..5257
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="CAP binding activates transcription in the presence of cAMP."
                     /bound_moiety="E. coli catabolite activator protein"
     primer_bind     complement(5374..5391)
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5545..6133)
                     /label="ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
                     /direction="LEFT"
     primer_bind     complement(5625..5644)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="pBR322 origin, forward primer"
     CDS             complement(6304..7164)
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="confers resistance to ampicillin, carbenicillin, andrelated antibiotics"
                     /gene="bla"
                     /product="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     misc_feature    6700..1041
                     /label="Piece A"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          6700..6729
                     /label="AmpR-for"
                     /note="sequence: ATCCCCCATGTTGTGCAAAAAAGCGGTTAG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(6700..6729)
                     /label="AmpR-rev"
                     /note="sequence: CTAACCGCTTTTTTGCACAACATGGGGGAT"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     6927..6946
                     /label="Amp-R"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7165..7269)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /gene="bla"
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgg gcggtggagg gagcaccctg agcgattata acattcagaa agaaagcacc
      961 ctgcatctgg tgctgcgcgg cggactcgag tccggactca gatctggcag cggtggaggc
     1021 gatatcatgg ccgagatcgg caccggcttc cccttcgacc cccactacgt ggaggtgctg
     1081 ggcgagcgca tgcactacgt tgacgtgggc ccccgcgacg gcacccccgt gctgttcctg
     1141 cacggcaacc ccaccagcag ctacgtgtgg cgcaacatca tcccccacgt ggcccccacc
     1201 caccgcacta tcgcccccga cctgatcggc atgggcaaga gcgacaagcc cgacctgggc
     1261 tacttcttcg acgaccacgt gcgcttcatg gacgccttca tcgaggccct gggcctggag
     1321 gaggtggtgc tggtgatcca cgactggggc agcgccctgg gcttccactg ggccaagcgc
     1381 aaccccgagc gcgtgaaggg catcgccttc atggagttca tccgccccat ccccacctgg
     1441 gacgagtggc ccgagttcgc ccgcgagacc ttccaggcct tccgcaccac cgacgtgggc
     1501 cgcaagctga tcatcgacca gaacgtgttc atcgagggca ccctgcccat gggcgtggtg
     1561 cgccccctga ccgaggtgga gatggaccac taccgcgagc ccttcctgaa ccccgtggac
     1621 cgcgagcccc tgtggcgctt ccccaacgag ctgcccatcg ccggcgagcc cgccaacatc
     1681 gtggccctgg tggaggagta catggactgg ctgcaccaga gccccgtgcc caagctgctg
     1741 ttctggggca cccccggcgt gctgatcccc cccgccgagg ccgcccgcct ggccaagagc
     1801 ctgcccaacg ttaaggccgt ggacatcggc cccggcctga acctgctcca ggaggacaac
     1861 cccgacctga tcggcagcga gatcgcccgc tggctgagca ccctcgagat cagcggcgga
     1921 tccggcagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggcagtgct
     1981 ggtggtagtg ctggtggtag tgctggtggc tctagaggag aaatcgcaaa gagtctgaaa
     2041 gaaattgcaa aatccttgaa ggaaatagcg tggtcactga aagaaattgc caagtccctc
     2101 aaagggggta ccgagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct
     2161 ggcccaaagc ttgccaccat gcatggtaat ggtaggtttt taactttaaa accattgcct
     2221 gacagcatta ttcaagaaag cctggagatt cagcaaggtg tgaatccatt tttcattggt
     2281 agatccgagg attgcaattg taaaattgaa gacagtaggt tgtctcgagt tcattgcttc
     2341 attttcaaaa agaggcatgc tgtaggcaaa agcatgtatg aatctccggc acaaggttta
     2401 gatgatattt ggtattgcca caccggaact aacgtgagct atttaaataa taaccgcatg
     2461 atacagggta cgaaattcct tttacaagac ggagatgaaa tcaagatcat ttgggataaa
     2521 aacaataaat ttgtcattgg ctttaaagtg gaaattaacg atactacagg tctgtttaac
     2581 gagggattag gtatgttaca agaacaaaga gtagtactta agcaaacagc cgaagaaaaa
     2641 gatttggtga aaaagttagg atccggcagt gctggtggta gtgctggtgg tagtgctggt
     2701 ggtagtgctg gtggcagtgc tggtggtagt gctggtggta gtgctggtgg ctctagaacc
     2761 ttgagagaaa tcgaggaatt gttgaggaaa atcattgaag acagcgtgcg cagtgtggcc
     2821 gagctggagg acattgagaa gtggctgaag aaaatctaag aattcgggcc cgtttaaacc
     2881 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     2941 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     3001 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     3061 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     3121 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
     3181 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     3241 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     3301 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     3361 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     3421 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     3481 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     3541 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
     3601 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
     3661 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
     3721 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
     3781 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     3841 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
     3901 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
     3961 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     4021 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     4081 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     4141 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     4201 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     4261 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     4321 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     4381 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     4441 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     4501 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     4561 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     4621 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     4681 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     4741 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     4801 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     4861 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     4921 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
     4981 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     5041 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     5101 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     5161 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     5221 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     5281 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     5341 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     5401 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     5461 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     5521 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     5581 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     5641 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     5701 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     5761 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     5821 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5881 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5941 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6001 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6061 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6121 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6181 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6241 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6301 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6361 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6421 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6481 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6541 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6601 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6661 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6721 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6781 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6841 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6901 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6961 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7021 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7081 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7141 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7201 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7261 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//