LOCUS       p23_vanR_pTec_op        4590 bp DNA     circular SYN 14-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Marc Blanch
  TITLE     Direct Submission
  JOURNAL   Exported Oct 14, 2025 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4590
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1778..2515
                     /codon_start=1
                     /label=Erythromycin Cassette
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
                     KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYN
                     LSTQIIKKVVFESRASDIYLTVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
                     PKPKVNSVLIKLTRHTTDVPDKYWKLYKYFVSKWVNREYRQLFTKNQFRQAMKHAKVNN
                     LSTITYEQVLSIFNSYLLFNGRK"
     misc_feature    2683..2711
                     /label=p23
     misc_feature    2734..2739
                     /label=RBS
     misc_feature    2749..3483
                     /label=vanR
     terminator      3492..3519
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    3598..3609
                     /label=vanO
     misc_feature    3616..3621
                     /label=-35
     misc_feature    3622..3638
                     /label=operator
     misc_feature    3639..3643
                     /label=-10
     misc_feature    3654..3665
                     /label=vanO
     misc_feature    3675..3680
                     /label=RBS
     CDS             3688..4398
                     /codon_start=1
                     /label=mCherry
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     terminator      4404..4451
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gctgcgagtc agtgagcgag aaggattatt cggctggttg
      781 agacgttaaa atgataaagg ttgtattaat cttatattac ggttataatg tactcaactt
      841 aataaatgaa cgcaaaaaaa agaaccctca acttagcaga gttaggattc acgacttatc
      901 agcacaacct gataagattt tcgatagcaa gtactaccaa tacaagctat ctaacttggt
      961 actattataa catgtaggct aagtttttca accattgata cttaaagtaa acggttgtta
     1021 tcgggaatct taacagaaac ctgatagcaa ccgttttttt gttattcaat ggttagcaac
     1081 catcaaagca actaaaggct ggaaacctgt tcttagctag taaaacctcc cgtgagtgtc
     1141 gttcgtgacc ccgcttgcag ttaacaacat aggtatgcta aaccttgtcg agatcaacgc
     1201 gactaaagac gtggctggaa gactaggaaa tgatacggac aggctaacta ttaacgcaga
     1261 ttattcgggt tgctgctaaa accaactcta ataatagtta gtgcaagggc tggttgagct
     1321 taaattgtct gataaagagt tctctcttta tactgcaaaa gaagcgcagt tattcacgat
     1381 taggataact gtttgagaga gcctaagggc ttgacccttg atggtttaag caccgctatg
     1441 cgtgcgggat ccccttagaa gcaaacttag agtgtgttga tagtgcatta tcttaaaatt
     1501 ttgtataata ggaattgaag ttaaattaga tgctaaaaat aggaattgaa gttaaattag
     1561 atgctaaaaa tttgtaatta agaaggaggg attcgtcatg ttggtattcc aaatgcgtaa
     1621 tgtagataaa acatctactg ttttgaaaca gactaaaaac agtgattacg cagataaata
     1681 aatacgttag attaattcct accagtgact aatcttatga ctttttaaac agataactaa
     1741 aattacaaac aaatcgttta acttcaggag agattacatg aacaaaaata taaaatattc
     1801 tcaaaacttt ttaacgagtg aaaaagtact caaccaaata ataaaacaat tgaatttaaa
     1861 agaaaccgat accgtttacg aaattggaac aggtaaaggg catttaacga cgaaactggc
     1921 taaaataagt aaacaggtaa cgtctattga attagacagt catctattca acttatcgtc
     1981 agaaaaatta aaactgaata ctcgtgtcac tttaattcac caagatattc tacagtttca
     2041 attccctaac aaacagaggt ataaaattgt tgggaatatt ccttacaatt taagcacaca
     2101 aattattaaa aaagtggttt ttgaaagccg tgcgtctgac atctatctga ctgttgaaga
     2161 aggattctac aagcgtacct tggatattca ccgaacacta gggttgctct tgcacactca
     2221 agtctcgatt cagcaattgc ttaagctgcc agcggaatgc tttcatccta aaccaaaagt
     2281 aaacagtgtc ttaataaaac ttacccgcca taccacagat gttccagata aatattggaa
     2341 gctatataag tactttgttt caaaatgggt caatcgagaa tatcgtcaac tgtttactaa
     2401 aaatcagttt cgtcaagcaa tgaaacacgc caaagtaaac aatttaagta ccattactta
     2461 tgagcaagta ttgtctattt ttaatagtta tctattattt aacgggagga aataattcta
     2521 tgagtcgctt ttttaaattt ggaaagttac acgttactaa agggaatgga gaccggagat
     2581 ctgcatcgta atagagcata gacgcataga tctcgctaga gatatagata gataagcgtt
     2641 cggtcagtaa ataatagaaa taaaaaatca gacctaagac tgatgacaaa aagagaaaat
     2701 tttgataaaa tagtcttaga attaaattaa aaagggaggc caaatataat ggacatgcct
     2761 cgtattaaac cgggtcagcg tgttatgatg gcactgcgta aaatgattgc aagcggtgaa
     2821 atcaaaagtg gtgaacgtat tgcagaaatt ccgaccgcag cagcactggg tgttagccgt
     2881 atgccggttc gtatcgcact gcgttcactg gaacaagaag gtctggttgt tcgtctgggt
     2941 gcacgtggtt atgcagcccg tggtgttagc agcgatcaga ttcgtgatgc aattgaagtt
     3001 cgtggtgttc tggaaggttt tgcagcacgt cgtctggcag aacgtggtat gaccgcagaa
     3061 acccatgcac gttttgttgt actgattgca gaaggtgaag cactgtttgc agccggtcgc
     3121 ctgaatggtg aagatctgga tcgttatgcc gcatataatc aggcatttca tgataccctg
     3181 gttagcgcag caggtaatgg tgcagttgaa agcgcactgg cacgtaatgg ttttgaaccg
     3241 tttgcagcag ccggtgcact ggccctggat ctgatggacc tgtctgccga atatgaacat
     3301 ctgctggcag cacatcgtca gcatcaggca gttctggatg cagttagctg tggtgatgcc
     3361 gaaggtgcag aacgtattat gcgtgatcat gcactggcag caattcgtaa tgcaaaagtt
     3421 tttgaagcag cagcaagcgc aggcgcaccg ctgggtgcag catggtcaat tcgtgcagat
     3481 tgataaaata aggctcacct tcgggtgggc ctttctgcgc tgcattaagc ttttaaccac
     3541 aagttgagca agagatcgtt cgtagagatc gaagaatagt tgctcggaat ttgagcaatt
     3601 ggatccaatt ttgtgttgac tcccggtcta gaacggggta ttattaaagc atcattggat
     3661 ccaataagaa cgaaaggaga aaacgaaatg gtttcaaagg gtgaagaaga taacatggct
     3721 atcatcaagg aattcatgcg tttcaaggtt cacatggaag gttcagttaa cggtcacgaa
     3781 ttcgaaatcg aaggtgaagg tgaaggtcgt ccatacgaag gtactcaaac tgctaagtta
     3841 aaggttacta agggtggtcc attaccattc gcttgggata tcttatcacc acaattcatg
     3901 tacggttcaa aggcttacgt taagcaccca gctgatatcc cagattactt aaagttatca
     3961 ttcccagaag gtttcaagtg ggaacgtgtt atgaacttcg aagatggtgg tgttgttact
     4021 gttactcaag attcatcatt acaagatggt gaattcatct acaaggttaa gttacgtggt
     4081 actaacttcc catcagatgg tccagttatg caaaagaaga ctatgggttg ggaagcttca
     4141 tcagaacgta tgtacccaga agatggtgct ttaaagggtg aaatcaagca acgtttaaag
     4201 ttaaaggatg gtggtcacta cgatgctgaa gttaagacta cttacaaggc taagaagcca
     4261 gttcaattac caggtgctta caacgttaac atcaagttag atatcacttc acacaacgaa
     4321 gattacacta tcgttgaaca atacgaacgt gctgaaggtc gtcactcaac tggtggtatg
     4381 gatgaattat acaagtaaaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     4441 ggggtttttt gctgaaagga ggaactatat ccggtagcgg cgcattaagc gggagcagaa
     4501 tgtccgagac taattcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     4561 agaccccgta gaaaagatca aaggatcttc
//
