LOCUS       Exported                1230 bp ds-DNA     linear   UNA 18-AUG-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 14, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    303..891
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(774..785)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(894..910)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 nnnnnnnnng actagtaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac
       61 gaagcttggg ctgcaggtcg actctagagg atcgaattca tgtcggctac cgctgccacc
      121 gtcccgcctg ccgccccggc cggcgagggt ggcccccctg cacctcctcc aaatcttacc
      181 agtaacagga gactgcagca gacccaggcc caggtggatg aggtggtgga catcatgagg
      241 gtgaatgtgg acaaggtcct ggagtaagga tccgttaaca attcgatatc aagcttatcg
      301 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
      361 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
      421 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
      481 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
      541 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
      601 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
      661 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
      721 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
      781 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
      841 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
      901 gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
      961 atgctgattg tgcctggcta gaagcacaag aggaggaggg aggtgggttt tccagtcaca
     1021 cctcnntacc tttaagacca atgacttacn aggcagctgt agatcttanc cactttttaa
     1081 aagaaaangg gggnctggnn gggctaattc actcccnacn aaganaannn annnngatnn
     1141 nnggntnnnc cannnnnnna ngntanntcc tgattggnan naacnnnnnn nccnnnnncc
     1201 nggnnnnnnn annnnccncn gngnnnnnnn
//
