LOCUS       Gal4_AD-LuxR_gen1       3459 bp ds-DNA     linear       15-JAN-2026
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..522
                     /label="X-4\UP"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="vntifkey: 21 ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    530..536
                     /label="Nt.BvCI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="vntifkey: 21 ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_binding    536..547
                     /label="MCS\1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="vntifkey: 20 note: Oriinal location: 1329..1371 ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    548..553
                     /label="Misc_Feature_1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="vntifkey: 21 note: Nb.BsmI ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     promoter        561..1546
                     /label="pPGK1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             1562..2686
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MANFNQSGNIADSSLSFTFTNSSKGPNLITTQTNSQALSQPIASSDVHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNSTTMDDVYNYLFDDEDTPPNPKEEPGPPKKKRKVENIDADDTYRIINKIKACRSNNDINQCLSDMTKMVHCEYYLLAIIYPHSMVKSDISILDNYPKKWRQYYDDANLIKYDPIVDYSISNHSPINWNIFENNAVNKKSPNVIKEAKTYGLITGFSFPIHTANNGFGMLSFAHSEKDNYIDSLFLHACMNIPLIVPYLVDNYRKINIANNKSNNDLTKREKECLAWACEGKSSRDISKILGCSERTVTFHLTNAQMKLNTTNRCQSISKAILTGAIDCPYFKN*"
     misc_feature    1565..1906
                     /label="GAL4_AD"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1916..1935
                     /label="NLS"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1936..2686
                     /label="LuxR_gen1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2696..2890
                     /label="tADH1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2891..2897)
                     /label="ID402pintRevU (ID402)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     primer          2898..2904
                     /label="ID401pIntFwdU (ID401)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2905..2922
                     /label=401
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    complement(2994..3013)
                     /label="TJOS-120"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    3000..3006
                     /label="Nt.BvcI"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="vntifkey: 21 label: Nt.BvcI(1) ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    3014..3459
                     /label="X-4\Down"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="vntifkey: 21 ApEinfo_revcolor: pink ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    3014..3033
                     /label="TJOS-116"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /note="ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
ORIGIN
        1 cccaaagcta agagtcccat tttattcttc tatatgtata ttttcgatac tctaaaccac
       61 cctacaatgt agccctatac taaatctgct caattttcag cttctacaag tgactcgagg
      121 ccacgtggaa agatccaact actccagcac aacgattcaa tataatcgat tgctccactc
      181 ataagaggca agaacaagct tcaacttttg gtaagccgcc gtttataaac agggaagatg
      241 tcctttgtca agggaggcac agagcatggc caatttggca aattgcaggt ttttctgagt
      301 gaaaaatgaa aaagcattgt agtagagtcg gctcactgaa aaaccgggga ggacgaaaag
      361 gtttccagcc acagttgtag tcacgtgcgc gccatgctga ctaatggcag ccgtcgttgg
      421 gcagaagaga attagtatgg tacaggatac gctaattgcg ctccaactac caaggttgtt
      481 gagggaacac tggggcaata ggctgtcgcc attcaagagc agattaagtc ctcagcgagc
      541 tcgcatggaa tgcgtgcgat CGAAGAAGTA CCTTCAAAGA ATGGGGTCTT ATCTTGTTTT
      601 GCAAGTACCA CTGAGCAGGA TAATAATAGA AATGATAATA TACTATAGTA GAGATAACGT
      661 CGATGACTTC CCATACTGTA ATTGCTTTTA GTTGTGTATT TTTAGTGTGC AAGTTTCTGT
      721 AAATCGATTA ATTTTTTTTT CTTTCCTCTT TTTATTAACC TTAATTTTTA TTTTAGATTC
      781 CTGACTTCAA CTCAAGACGC ACAGATATTA TAACATCTGC ATAATAGGCA TTTGCAAGAA
      841 TTACTCGTGA GTAAGGAAAG AGTGAGGAAC TATCGCATAC CTGCATTTAA AGATGCCGAT
      901 TTGGGCGCGA ATCCTTTATT TTGGCTTCAC CCTCATACTA TTATCAGGGC CAGAAAAAGG
      961 AAGTGTTTCC CTCCTTCTTG AATTGATGTT ACCCTCATAA AGCACGTGGC CTCTTATCGA
     1021 GAAAGAAATT ACCGTCGCTC GTGATTTGTT TGCAAAAAGA ACAAAACTGA AAAAACCCAG
     1081 ACACGCTCGA CTTCCTGTCT TCCTATTGAT TGCAGCTTCC AATTTCGTCA CACAACAAGG
     1141 TCCTAGCGAC GGCTCACAGG TTTTGTAACA AGCAATCGAA GGTTCTGGAA TGGCGGGAAA
     1201 GGGTTTAGTA CCACATGCTA TGATGCCCAC TGTGATCTCC AGAGCAAAGT TCGTTCGATC
     1261 GTACTGTTAC TCTCTCTCTT TCAAACAGAA TTGTCCGAAT CGTGTGACAA CAACAGCCTG
     1321 TTCTCACACA CTCTTTTCTT CTAACCAAGG GGGTGGTTTA GTTTAGTAGA ACCTCGTGAA
     1381 ACTTACATTT ACATATATAT AAACTTGCAT AAATTGGTCA ATGCAAGAAA TACATATTTG
     1441 GTCTTTTCTA ATTCGTAGTT TTTCAAGTTC TTAGATGCTT TCTTTTTCTC TTTTTTACAG
     1501 ATCATCAAGG AAGTAATTAT CTACTTTTTA CAACAAATAT AAAACAACCT GCACTAAAAC
     1561 AATGGCCAAT TTTAATCAAA GTGGGAATAT TGCTGATAGC TCATTGTCCT TCACTTTCAC
     1621 TAACAGTAGC AAAGGTCCGA ACCTCATAAC AACTCAAACA AATTCTCAAG CGCTTTCACA
     1681 ACCGATTGCC TCCTCTGACG TTCATGATAA CTTCATGAAT AATGAAATCA CGGCTAGTAA
     1741 AATTGATGAT GGTAATAATT CAAAACCACT GTCACCTGGT TGGACGGACC AAACTGCGTA
     1801 TAACGCGTTT GGAATCACTA CAGGGATGTT TAATTCCACT ACAATGGATG ATGTTTATAA
     1861 CTATCTATTC GATGATGAAG ATACCCCACC AAACCCAAAA GAAGAGCCAG GTCCACCAAA
     1921 AAAGAAGAGA AAGGTTGAAA ACATCGACGC TGATGACACC TACAGAATCA TCAACAAGAT
     1981 TAAGGCTTGC AGGTCTAACA ACGATATTAA CCAGTGTTTG TCCGACATGA CCAAGATGGT
     2041 CCATTGCGAA TATTACTTGT TGGCCATCAT CTACCCACAC TCTATGGTTA AGTCCGACAT
     2101 TTCCATTTTG GACAACTACC CAAAAAAATG GCGTCAGTAT TACGATGATG CCAACTTGAT
     2161 TAAGTACGAC CCAATCGTTG ATTACTCCAT CTCTAATCAT TCTCCCATCA ACTGGAACAT
     2221 CTTCGAAAAC AATGCCGTCA ACAAAAAGTC CCCAAACGTT ATCAAAGAAG CTAAGACCTA
     2281 TGGTTTGATT ACCGGTTTTT CTTTCCCAAT TCATACCGCT AACAATGGTT TCGGTATGTT
     2341 GTCTTTTGCC CACTCTGAAA AGGATAACTA CATCGACTCA TTATTCTTGC ATGCCTGCAT
     2401 GAACATTCCA TTGATAGTTC CATATCTGGT CGATAACTAT AGGAAGATTA ACATTGCCAA
     2461 CAACAAGTCC AACAACGACT TGACCAAAAG GGAAAAAGAA TGTTTGGCTT GGGCTTGCGA
     2521 AGGTAAATCT TCTAGGGATA TTTCCAAGAT TTTGGGTTGC TCTGAAAGAA CCGTTACTTT
     2581 CCATTTGACT AACGCCCAAA TGAAGTTGAA CACTACCAAC AGATGCCAGT CTATTTCTAA
     2641 GGCTATTTTG ACCGGTGCTA TTGATTGCCC ATACTTTAAG AATTAAAGGA CAGATgtaga
     2701 tacgttgttg acacttctaa ataagcgaat ttcttatgat ttatgatttt tattattaaa
     2761 taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt tttaaaacga
     2821 aaattcttat tcttgagtaa ctctttcctg taggtcaggt tgctttctca ggtatagcat
     2881 gaggtcgctc ATCGCGTACC CAATtcgccc tatagtgagt cgtattacgc gcgctcactg
     2941 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccctgc aggactagtg
     3001 ctgaggcatt aatacatggg aagattcgct tttttttttt gaattacaat agtatgtctg
     3061 atgtctgcaa gaagtaacag gcgtgtgcac aagaatacgt gtgtgtgcgt aagcgtatgc
     3121 actggtggca taacttatct aagaagtata tatcactgac atagaaatgt agatatacag
     3181 gtatttttct cgataatcga taaaaatctc gtcgcgctga accaaacttg gtggttacgg
     3241 agagtttttc tctcatcatt actgtctttc gcattgattt cccctttgac cgataaaatc
     3301 ccttggattc ataagattaa acaaagaggt gatcaaagag aaccctgtga aagtttatgt
     3361 ttataaccgg gcataaagtg aactagacac tttcaagaag ccaaccaaag catgagtaac
     3421 gaagcttacc agcatgatca taccgtaaat cctcaccag
//