LOCUS       168_pB-HERVK-pro       11732 bp DNA     circular SYN 27-NOV-2024
DEFINITION  Rescue_mini5.
ACCESSION   Rescue_mini5
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 15, 2025 from SnapGene 7.0.2
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..11732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(171..1031)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1032..1136)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      1189..1617
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(1622..1777)
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
                     /translation="SLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQ
                     LRSLNGE"
     CDS             1779..1898
                     /codon_start=1
                     /label=B (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.5"
                     /note="/other=CDS"
                     /translation="RASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQL"
     primer_bind     1843..1862
                     /label=BII(R)
                     /note="BII(R)"
     repeat_region   1905..1917
                     /label=3'TR
                     /note="3'TR"
     repeat_region   1918..1948
                     /label=3'TR(1)
                     /note="3'TR(1)"
     enhancer        2143..2374
                     /note="core insul"
     misc_feature    2398..2515
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        2562..2773
                     /label=EF-1-alpha core promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    2847..3373
                     /label=eif1a promoter
                     /note="eif1a promoter"
     CDS             3583..5412
                     /codon_start=1
                     /label=ERVK-6 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=99.5"
                     /note="/match_length=91.6"
                     /note="/other=CDS"
                     /translation="LKDWKRIGKELKQAGRKGNIIPLTVWNDWAIIKAALEPFQTEEDS
                     VSVSDAPGSCIIDCNENTRKKSQKETEGLHCEYVAEPVMAQSTQNVDYNQLQEVIYPET
                     LKLEGKGPELVGPSESKPRGTSPLPAGQVPVTLQPQKQVKENKTQPPVAYQYWPPAELQ
                     YRPPPESQYGYPGMPPAPQGRAPYPQPPTRRLNPTAPPSRQGSELHEIIDKSRKEGDTE
                     AWQFPVTLEPMPPGEGAQEGEPPTVEARYKSFSIKMLKDMKEGVKQYGPNSPYMRTLLD
                     SIAHGHRLIPYDWEILAKSSLSPSQFLQFKTWWIDGVQEQVRRNRAANPPVNIDADQLL
                     GIGQNWSTISQQALMQNEAIEQVRAICLRAWEKIQDPGSTCPSFNTVRQGSKEPYPDFV
                     ARLQDVAQKSIADEKARKVIVELMAYENANPECQSAIKPLKGKVPAGSDVISEYVKACD
                     GIGGAMHKAMLMAQAITGVVLGGQVRTFGGKCYNCGQIGHLKKNCPVLNKQNITIQATT
                     TGREPPDLCPRCKKGKHWASQCRSKFDKNGQPLSGNEQRGQPQAPQQTGAFPIQPFVPQ
                     GFQGQQPPLSQVFQGISQLPQYNNCPPPQAAVQQ"
     misc_feature    4103..5415
                     /label=GAG
     misc_feature    5215..6221
                     /label=PRO
     misc_feature    6182..8942
                     /label=POL
     misc_feature    8949..9476
                     /label=eif1a promoter
                     /note="eif1a promoter"
     CDS             9505..10206
                     /codon_start=1
                     /label=TagBFP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     repeat_region   10217..10235
                     /label=3'TR
                     /note="3'TR"
     repeat_region   10221..10251
                     /label=5'TR
                     /note="5'TR"
     repeat_region   10239..10251
                     /label=3'TR
                     /note="3'TR"
     CDS             10261..10728
                     /codon_start=1
                     /label=B (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=29.3"
                     /note="/other=CDS"
                     /translation="LHQDHIVGSFFRLSHRPSWRYLGIGEEEARAFSREVEAAWKEFAE
                     DDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTSSSRLFRTQFRMVSPKRISN
                     PNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQKWTWIPRELPGGRA"
     protein_bind    10764..10786
                     /label=lac operator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=92.0"
                     /note="/other=protein_bind"
     primer_bind     complement(10770..10789)
                     /label=pLac(F)
                     /note="pLac(F)"
     promoter        10790..10820
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    10835..10856
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(10869..10961)
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      11144..11732
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
ORIGIN
        1 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
       61 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
      121 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
      181 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      241 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      301 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      361 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
      421 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
      481 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
      541 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
      601 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
      661 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
      721 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
      781 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
      841 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      901 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      961 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1021 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1081 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1141 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     1201 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     1261 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     1321 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     1381 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     1441 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     1501 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     1561 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     1621 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1681 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1741 tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcctcgttc attcacgttt
     1801 ttgaacccgt ggaggacggg cagactcgcg gtgcaaatgt gttttacagc gtgatggagc
     1861 agatgaagat gctcgacacg ctgcagaaca cgcagctaga ttaaccctag aaagataatc
     1921 atattgtgac gtacgttaaa gataatcatg tgtaaaattg acgcatgtgt tttatcggtc
     1981 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
     2041 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
     2101 caaaaactca aaatttcttc tataaagtaa caaaactttt atgagggaca gccccccccc
     2161 aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc gagccgcccg
     2221 gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt gcggggacag
     2281 cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc gctgctcttt
     2341 gagcctgcag acacctgggg ggatacgggg aaaaggcctc cacggccaga ctagaacttt
     2401 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     2461 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     2521 tcacgagact agcctcgagg gatcatccgg tgcccgtcag tgggcagagc gcacatcgcc
     2581 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg
     2641 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     2701 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     2761 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc
     2821 tacctgaggc cgccatccac gccgattccg gtgcccgtca gtgggcagag cgcacatcgc
     2881 ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc tagagaaggt
     2941 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     3001 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     3061 ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc gcccgccgcc
     3121 ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc gcctgtggtg
     3181 cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga ccgggccttt
     3241 gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt tgcctgaccc
     3301 tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac agatccaagc
     3361 tgtgaccggc gcctcttcgc atattaaggt gacgcgtgtg gcctcgaaca ccgagcgggc
     3421 aaactaaaag taaaattaaa agtaaatatg cctcttatct cagctttatt aaaattcttt
     3481 taaaaagagg gggagttaaa gtatctacaa aaaatctaat caagctattt caaataatag
     3541 aacaattttg cccatggttt ccagaacaag gaactttaga ttctaaaaga ttggaaaaga
     3601 attggtaagg aactaaaaca agcaggtagg aagggtaata tcattccact tacagtatgg
     3661 aatgattggg ccattattaa agcagcttta gaaccatttc aaacagaaga agatagcgtt
     3721 tcagtttctg atgcccctgg aagctgtata atagattgta atgaaaacac aaggaaaaaa
     3781 tcccagaaag aaacggaagg tttacattgc gaatatgtag cagagccggt aatggctcag
     3841 tcaacgcaaa atgttgacta taatcaatta caggaggtga tatatcctga aacgttaaaa
     3901 ttagaaggaa aaggtccaga attagtgggg ccatcagagt ctaaaccacg aggcacaagt
     3961 cctcttccag caggtcaggt gcccgtaaca ttacaacctc aaaagcaggt taaagaaaat
     4021 aagacccaac cgccagtagc ctatcaatac tggcctccgg ctgaacttca gtatcggcca
     4081 cccccagaaa gtcagtatgg atatccagga atgcccccag caccacaggg cagggcgcca
     4141 taccctcagc cgcccactag gagacttaat cctacggcac cacctagtag acagggtagt
     4201 gaattacatg aaattattga taaatcaaga aaggaaggag atactgaggc atggcaattc
     4261 ccagtaacgt tagaaccgat gccacctgga gaaggagccc aagagggaga gcctcccaca
     4321 gttgaggcca gatacaagtc tttttcgata aaaatgctaa aagatatgaa agagggagta
     4381 aaacagtatg gacccaactc cccttatatg aggacattat tagattccat tgctcatgga
     4441 catagactca ttccttatga ttgggagatt ctggcaaaat cgtctctctc accctctcaa
     4501 tttttacaat ttaagacttg gtggattgat ggggtacaag aacaggtccg aagaaatagg
     4561 gctgccaatc ctccagttaa catagatgca gatcaactat taggaatagg tcaaaattgg
     4621 agtactatta gtcaacaagc attaatgcaa aatgaggcca ttgagcaagt tagagctatc
     4681 tgccttagag cctgggaaaa aatccaagac ccaggaagta cctgcccctc atttaataca
     4741 gtaagacaag gttcaaaaga gccctatcct gattttgtgg caaggctcca agatgttgct
     4801 caaaagtcaa ttgccgatga aaaagcccgt aaggtcatag tggagttgat ggcatatgaa
     4861 aacgccaatc ctgagtgtca atcagccatt aagccattaa aaggaaaggt tcctgcagga
     4921 tcagatgtaa tctcagaata tgtaaaagcc tgtgatggaa tcggaggagc tatgcataaa
     4981 gctatgctta tggctcaagc aataacagga gttgttttag gaggacaagt tagaacattt
     5041 ggaggaaaat gttataattg tggtcaaatt ggtcacttaa aaaagaattg cccagtctta
     5101 aacaaacaga atataactat tcaagcaact acaacaggta gagagccacc tgacttatgt
     5161 ccaagatgta aaaaaggaaa acattgggct agtcaatgtc gttctaaatt tgataaaaat
     5221 gggcaaccat tgtcgggaaa cgagcaaagg ggccagcctc aggccccaca acaaactggg
     5281 gcattcccaa ttcagccatt tgttcctcag ggttttcagg gacaacaacc cccactgtcc
     5341 caagtgtttc agggaataag ccagttacca caatacaaca attgtccccc gccacaagcg
     5401 gcagtgcagc agtagattta tgtactatac aagcagtctc tctgcttcca ggggagcccc
     5461 cacaaaaaat ccccacaggg gtatatggcc ccctgcctga ggggactgta ggactaatct
     5521 tgggaagatc aagtctaaat ctaaaaggag ttcaaattca tactagtgtg gttgattcag
     5581 actataaagg cgaaattcaa ttggttatta gctcttcaat tccttggagt gccagtccag
     5641 gagacaggat tgctcaatta ttactcctgc catatattaa gggtggaaat agtgaaataa
     5701 aaagaatagg agggcttgga agcactgatc caacaggaaa ggctgcatat tgggcaagtc
     5761 aggtctcaga gaacagacct gtgtgtaagg ccattattca aggaaaacag tttgaagggt
     5821 tggtagacac tggagcagat gtctctatca ttgctttaaa tcagtggcca aaaaattggc
     5881 ctaaacaaaa ggctgttaca ggacttgtcg gcataggcac agcctcagaa gtgtatcaaa
     5941 gtacggagat tttacattgc ttagggccag ataatcaaga aagtactgtt cagccaatga
     6001 ttacttcaat tcctcttaat ctgtggggtc gagatttatt acaacaatgg ggtgcggaaa
     6061 tcaccatgcc cgctccatta tatagcccca cgagtcaaaa aatcatgacc aagatgggat
     6121 atataccagg aaagggacta gggaaaaatg aagatggcat taaagttcca gttgaggcta
     6181 aaataaatca agaaagagaa ggaatagggt atccttttta ggggcggcca ctgtagagcc
     6241 tcctaaaccc ataccattaa cttggaaaac agaaaaaccg gtgtgggtaa atcagtggcc
     6301 gctaccaaaa caaaaactgg aggctttaca tttattagca aatgaacagt tagaaaaggg
     6361 tcatattgag ccttcgttct caccttggaa ttctcctgtg tttgtaattc agaagaaatc
     6421 aggcaaatgg cgtatgttaa ctgacttaag ggctgtaaac gccgtaattc aacccatggg
     6481 gcctctccaa cccgggttgc cctctccggc catgatccca aaagattggc ctttaattat
     6541 aattgatcta aaggattgct tttttaccat ccctctggca gagcaggatt gcgaaaaatt
     6601 tgcctttact ataccagcca taaataataa agaaccagcc accaggtttc agtggaaagt
     6661 gttacctcag ggaatgctta atagtccaac tatttgtcag acttttgtag gtcgagctct
     6721 tcaaccagtt agagaaaagt tttcagactg ttatattatt cattatattg atgatatttt
     6781 atgtgctgca gaaacgaaag ataaattaat tgactgttat acatttctgc aagcagaggt
     6841 tgccaatgct ggactggcaa tagcatctga taagatccaa acctctactc cttttcatta
     6901 tttagggatg cagatagaaa atagaaaaat taagccacaa aaaatagaaa taagaaaaga
     6961 cacattaaaa acactaaatg attttcaaaa attactagga gatattaatt ggattcggcc
     7021 aactctaggc attcctactt atgccatgtc aaatttgttc tctatcttaa gaggagactc
     7081 agacttaaat agtaaaagaa tgttaacccc agaggcaaca aaagaaatta aattagtgga
     7141 agaaaaaatt cagtcagcgc aaataaatag aatagatccc ttagccccac tccaactttt
     7201 gatttttgcc actgcacatt ctccaacagg catcattatt caaaatactg atcttgtgga
     7261 gtggtcattc cttcctcaca gtacagttaa gacttttaca ttgtacttgg atcaaatagc
     7321 tacattaatc ggtcagacaa gattacgaat aataaaatta tgtggaaatg acccagacaa
     7381 aatagttgtc cctttaacca aggaacaagt tagacaagcc tttatcaatt ctggtgcatg
     7441 gcagattggt cttgctaatt ttgtgggaat tattgataat cattacccaa aaacaaagat
     7501 cttccagttc ttaaaattga ctacttggat tctacctaaa attaccagac gtgaaccttt
     7561 agaaaatgct ctaacagtat ttactgatgg ttccagcaat ggaaaagcag cttacacagg
     7621 gccgaaagaa cgagtaatca aaactccata tcaatcggct caaagagcag agttggttgc
     7681 agtcattaca gtgttacaag attttgacca acctatcaat attatatcag attctgcata
     7741 tgtagtacag gctacaaggg atgttgagac agctctaatt aaatatagca tggatgatca
     7801 gttaaaccag ctattcaatt tattacaaca aactgtaaga aaaagaaatt tcccatttta
     7861 tattactcat attcgagcac acactaattt accagggcct ttgactaaag caaatgaaca
     7921 agctgactta ctggtatcat ctgcactcat aaaagcacaa gaacttcatg ctttgactca
     7981 tgtaaatgca gcaggattaa aaaacaaatt tgatgtcaca tggaaacagg caaaagatat
     8041 tgtacaacat tgcacccagt gtcaagtctt acacctgccc actcaagagg caggagttaa
     8101 tcccagaggt ctgtgtccta atgcattatg gcaaatggat gtcacgcatg taccttcatt
     8161 tggaagatta tcatatgttc atgtaacagt tgatacttat tcacatttca tatgggcaac
     8221 ttgccaaaca ggagaaagta cttcccatgt taaaaaacat ttattgtctt gttttgctgt
     8281 aatgggagtt ccagaaaaaa tcaaaactga caatggacca ggatattgta gtaaagcttt
     8341 ccaaaaattc ttaagtcagt ggaaaatttc acatacaaca ggaattcctt ataattccca
     8401 aggacaggcc atagttgaaa gaactaatag aacactcaaa actcaattag ttaaacaaaa
     8461 agaaggggga gacagtaagg agtgtaccac tcctcagatg caacttaatc tagcactcta
     8521 tactttaaat tttttaaaca tttatagaaa tcagactact acttctgcag aacaacatct
     8581 tactggtaaa aagaacagcc cacatgaagg aaaactaatt tggtggaaag ataataaaaa
     8641 taagacatgg gaaataggga aggtgataac gtgggggaga ggttttgctt gtgtttcacc
     8701 aggagaaaat cagcttcctg tttggatacc cactagacat ttgaagttct acaatgaacc
     8761 catcggagat gcaaagaaaa gcacctccgc ggagacggag acaccgcaat cgagcaccgt
     8821 tgactcacaa gatgaacaaa atggtgacgt cagaagaaca gatgaagttg ccatccacca
     8881 agaaggcaga gccgccgact tgggcacaac taaagaagct gacgcagtta gctacaaaat
     8941 atctagacat ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     9001 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg
     9061 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     9121 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg
     9181 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat
     9241 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc
     9301 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg
     9361 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac
     9421 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgccgaat
     9481 tcttcgcata ttaaggtgac gcgtatgagc gagctgatta aggagaacat gcacatgaag
     9541 ctgtacatgg agggcaccgt ggacaaccat cacttcaagt gcacatccga gggcgaaggc
     9601 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
     9661 ttcgccttcg acatcctggc tactagcttc ctctacggca gcaagacctt catcaaccac
     9721 acccagggca tccccgactt cttcaagcag tccttccctg agggcttcac atgggagaga
     9781 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
     9841 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcacatccaa cggccctgtg
     9901 atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga cgctgtaccc cgctgacggc
     9961 ggcctggaag gcagaaacga catggccctg aagctcgtgg gcgggagcca tctgatcgca
    10021 aacatcaaga ccacatatag atccaagaaa cccgctaaga acctcaagat gcctggcgtc
    10081 tactatgtgg actacagact ggaaagaatc aaggaggcca acaacgagac ctacgtcgag
    10141 cagcacgagg tggcagtggc cagatactgc gacctcccta gcaaactggg gcacaagctt
    10201 aattaaggag ctcatgcatg cgtcaatttt acgcagacta tctttctagg gttaatctag
    10261 ctgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc aagctggcgc
    10321 tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga agcggcatgg
    10381 aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg cacgtttacc
    10441 atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc
    10501 acctgggata ccagttcgtc gcggcttttc cggacacagt tccggatggt cagcccgaag
    10561 cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg tgtgcagatt
    10621 aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc tggctggatg
    10681 ccgcagaaat ggacatggat accccgtgag ttacccggcg ggcgcgcttg gcgtaatcat
    10741 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
    10801 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
    10861 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
    10921 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
    10981 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    11041 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    11101 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    11161 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    11221 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    11281 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    11341 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    11401 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    11461 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    11521 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    11581 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    11641 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    11701 agcagattac gcgcagaaaa aaaggatctc aa
//
