LOCUS       plasmid                 7632 bp    DNA     circular SYN 04-OCT-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     rep_origin      6072..6660
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             2377..4410
                     /note="pLannotate"
                     /label="DHX58"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      4588..5016
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(7087..7278)
                     /note="pLannotate"
                     /label="rop"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(455..1537)
                     /note="pLannotate"
                     /label="lacI"
                     /database="snapgene"
                     /identity="99.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(5135..5950)
                     /note="pLannotate"
                     /label="KanR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(1538..1615)
                     /note="pLannotate"
                     /label="lacI promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     terminator      4504..4551
                     /note="pLannotate"
                     /label="T7 terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     CDS             2080..2372
                     /note="pLannotate"
                     /label="SUMO (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.7"
                     /fragment="True"
                     /other="CDS"
     protein_bind    1943..1967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     RBS             1982..2004
                     /note="pLannotate"
                     /label="RBS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="RBS"
     promoter        1924..1942
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             2050..2067
                     /note="pLannotate"
                     /label="thrombin site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(6846..6985)
                     /note="pLannotate"
                     /label="bom"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     CDS             complement(115..406)
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="24.5"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(1623..1902)
                     /note="pLannotate"
                     /label="TcR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="23.5"
                     /fragment="True"
                     /other="CDS"
     promoter        5069..5126
                     /note="pLannotate"
                     /label="AmpR promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="56.3"
                     /fragment="True"
                     /other="promoter"
     CDS             join(7295..7632,1..43)
                     /note="pLannotate"
                     /label="YPB1_ECOLX"
                     /database="swissprot"
                     /identity="99.2"
                     /match_length="96.2"
                     /fragment="False"
                     /other="CDS"
     protein_bind    complement(421..442)
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="95.5"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             4447..4496
                     /note="pLannotate"
                     /label="SET1 (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="39.7"
                     /fragment="True"
                     /other="CDS"
     CDS             4420..4437
                     /note="pLannotate"
                     /label="9xHis (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="66.7"
                     /fragment="True"
                     /other="CDS"
     CDS             2023..2039
                     /note="pLannotate"
                     /label="capTEV™ (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="6.0"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
       61 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
      121 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
      181 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
      241 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
      301 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
      361 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
      421 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
      481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
      541 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
      601 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
      661 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
      721 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
      781 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
      841 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
      901 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
      961 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     1021 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     1081 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     1141 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     1201 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     1261 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     1321 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     1381 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     1441 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     1501 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct
     1561 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga
     1621 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg
     1681 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc
     1741 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
     1801 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
     1861 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
     1921 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     1981 ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
     2041 agcagcggcc tggtgccgcg cggcagccat atggctagca tgtcggactc agaagtcaat
     2101 caagaagcta agccagaggt caagccagaa gtcaagcctg agactcacat caatttaaag
     2161 gtgtccgatg gatcttcaga gatcttcttc aagatcaaaa agaccactcc tttaagaagg
     2221 ctgatggaag cgttcgctaa aagacagggt aaggaaatgg actccttaag attcttgtac
     2281 gacggtatta gaattcaagc tgatcagacc cctgaagatt tggacatgga ggataacgat
     2341 attattgagg ctcacagaga acagattggt ggatccatgg aactgcgtag ctatcaatgg
     2401 gaagttatta tgccggcgct ggagggtaaa aacatcatca tctggctgcc gaccggtgcg
     2461 ggcaaaaccc gtgcggcggc gtatgttgcg aagcgtcacc tggaaaccgt ggatggtgcg
     2521 aaagtggttg tgctggtgaa ccgtgttcac ctggtgaccc agcacggcga ggaattccgt
     2581 cgtatgctgg atggccgttg gaccgttacc accctgagcg gtgacatggg tccgcgtgcg
     2641 ggttttggtc atctggcgcg ttgccatgac ctgctgatct gcaccgcgga actgctgcag
     2701 atggcgctga ccagcccgga ggaagaggaa cacgttgaac tgaccgtgtt cagcctgatc
     2761 gttgtggatg agtgccacca cacccacaaa gacaccgttt acaacgtgat tatgagccaa
     2821 tatctggagc tgaagctgca acgtgcgcag ccgctgccgc aggtgctggg tctgaccgcg
     2881 agcccgggta ccggtggcgc gagcaaactg gatggcgcga tcaaccacgt tctgcaactg
     2941 tgcgcgaacc tggacacctg gtgcattatg agcccgcaga actgctgccc gcagctgcaa
     3001 gaacacagcc agcaaccgtg caagcaatac aacctgtgcc accgtcgtag ccaggacccg
     3061 ttcggtgatc tgctgaagaa actgatggac caaattcacg atcacctgga gatgccggaa
     3121 ctgagccgta aatttggcac ccagatgtat gaacagcaag ttgtgaagct gagcgaggcg
     3181 gcggcgctgg cgggtctgca ggagcaacgt gtgtacgcgc tgcacctgcg tcgttataac
     3241 gacgcgctgc tgatccacga taccgttcgt gcggtggatg cgctggcggc gctgcaagat
     3301 ttctatcacc gtgaacacgt taccaaaacc cagattctgt gcgcggagcg tcgtctgctg
     3361 gcgctgtttg acgatcgtaa gaacgaactg gcgcacctgg cgacccatgg tccggagaac
     3421 ccgaaactgg agatgctgga aaagatcctg cagcgtcaat tcagcagcag caacagcccg
     3481 cgtggcatca tttttacccg tacccgtcag agcgcgcaca gcctgctgct gtggctgcag
     3541 caacagcaag gtctgcaaac cgtggacatc cgtgcgcagc tgctgattgg tgcgggcaac
     3601 agcagccaaa gcacccacat gacccagcgt gatcagcaag aagttattca gaaattccaa
     3661 gacggcaccc tgaacctgct ggttgcgacc agcgtggcgg aggaaggtct ggatatcccg
     3721 cactgcaacg ttgtggttcg ttacggcctg ctgaccaacg agattagcat ggtgcaagcg
     3781 cgtggtcgtg cgcgggcgga ccagagcgtt tatgcgtttg tggcgaccga gggtagccgt
     3841 gaactgaagc gtgagctgat caacgaggcg ctggaaaccc tgatggaaca ggcggttgcg
     3901 gcggtgcaga aaatggatca agcggagtac caggcgaaga ttcgtgacct gcagcaagcg
     3961 gcgctgacca agcgtgcggc gcaggcggcg caacgtgaaa accaacgtca gcaattcccg
     4021 gttgagcacg tgcagctgct gtgcatcaac tgcatggtgg cggttggtca tggcagcgac
     4081 ctgcgtaagg tggagggcac ccaccacgtg aacgttaacc cgaacttcag caactactat
     4141 aacgttagcc gtgatccggt ggttatcaac aaggttttta aagactggaa gccgggtggc
     4201 gtgattagct gccgtaactg cggtgaagtt tggggcctgc agatgatcta taaaagcgtt
     4261 aagctgccgg ttctgaaagt gcgtagcatg ctgctggaaa ccccgcaggg tcgtattcag
     4321 gcgaagaaat ggagccgtgt tccgtttagc gtgccggatt ttgatttcct gcaacactgc
     4381 gcggagaacc tgagcgacct gagcctggac taactcgagc accaccacca ccaccactga
     4441 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     4501 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
     4561 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     4621 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4681 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4741 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4801 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4861 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4921 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     4981 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5041 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5101 acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat
     5161 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     5221 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     5281 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     5341 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt
     5401 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     5461 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
     5521 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     5581 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     5641 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     5701 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     5761 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     5821 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     5881 ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa
     5941 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     6001 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6061 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6121 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6181 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6241 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6301 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6361 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6421 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6481 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6541 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6601 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6661 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6721 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6781 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6841 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
     6901 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
     6961 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
     7021 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     7081 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
     7141 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
     7201 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
     7261 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
     7321 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
     7381 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
     7441 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag
     7501 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
     7561 ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga
     7621 cgttttgcag ca
//
