LOCUS       Genome_sequence_SHARK7  2470 bp ds-DNA     linear       06-SEP-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(1..2470)
                     /label="KO-LacI-NoSpecR"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    complement(1..200)
                     /label="LacZ"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    151..200
                     /label="Homology1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(205..262)
                     /label="MoClo-CIDAR - L3S3P21**"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(267..1184)
                     /label="Pir116"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             complement(267..1184)
                     /label="pir116"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MRLKVMMDVNKKTKIRHRNELNHTLAQLPLPAKRVMYMALAPIDSKEPLERGRVFKIRAEDLAALAKITPSLAYRQLKEGGKLLGASKISLRGDDIIALAKELNLLFTAKNSPEELDLNIIEWIAYSNDEGYLSLKFTRTIEPYISSLIGKKNKFTTQLLTASLRLSSQYSSSLYQLIRKHYSNFKKKNYFIISVDELKEELIAYTFDKDGNIEYKYPDFPIFKRDVLNKAIAEIKKKTEISFVGFTVHEKEGRKISKLKFEFVVDEDEFSGDKDDEAFFMNLSEADAAFLKVFDETVPPKKAKG*"
     misc_feature    complement(867..869)
                     /label="P106L"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(1192..1203)
                     /label="B0064"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    complement(1212..1286)
                     /label="RiboJ"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_RNA        complement(1235..1284)
                     /label="sTRSV HHRz"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="hammerhead ribozyme from the tobacco ringspot virus satellite RNA (Khvorova et al., 2003)"
     misc_feature    complement(1291..1344)
                     /label="PTetV"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(1297..1302)
                     /label=-10
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(1301..1319)
                     /label="TetO"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(1320..1325)
                     /label=-35
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    complement(1326..1344)
                     /label="TetO"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(1345..1348)
                     /label="ScarB"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(1349..1438)
                     /label="T12m - ECK120029600"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1443..2156)
                     /label="Lambda-CI"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(1443..2156)
                     /label="-lambda repressor"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /gene="cI"
                     /product="phage lambda repressor"
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQSGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEYPVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPDGMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSVVGKVIASQWPEETFG*"
     misc_feature    complement(2158..2184)
                     /label="Bujard"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     RBS             complement(2164..2175)
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(2189..2223)
                     /label="J23101"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    2228..2261
                     /label="FRT-Scar"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(2262..2280)
                     /label="Scar"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(2281..2292)
                     /label="PlacI-promoter5'"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2281..2330
                     /label="Homology2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(2293..2470)
                     /label="mhpR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 gtcggattct ccgtgggaac aaacggcgga ttgaccgtaa tgggataggt cacgttggtg
       61 tagatgggcg catcgtaacc gtgcatctgc cagtttgagg ggacgacgac agtatcggcc
      121 tcaggaagat cgcactccag ccagctttcc ggcaccgctt ctggtgccgg aaaccaggca
      181 aagcgccatt cgccattcag AAGCgagggc agaccagaaa caaaaaaagg ccccccgtta
      241 gggaggcctt caataattgg ccACCTtcac cccttagctt ttttgggagg tacggtttca
      301 tcaaatacct tgagaaaagc tgcatcagct tcagataaat tcataaaaaa agcttcatca
      361 tctttatcgc cagaaaattc atcttcatca acgacaaatt cgaacttcag cttactaatt
      421 tttcttcctt ctttttcatg aacagtgaag ccaacaaacg atatttctgt tttcttttta
      481 atttcagcaa tggctttatt taacacatcc cttttaaaaa taggaaagtc agggtatttg
      541 tactcaatat ttccatcttt atcaaaagta taagctatta actcttcctt taactcatca
      601 acggaaataa taaaataatt tttcttctta aaattagagt aatgcttcct gataagttga
      661 taaagagaag atgaatactg gctacttaag cgtaagcttg ccgttaacaa ttgcgttgtg
      721 aatttatttt ttttcccaat aaggctagag atatatggtt ctatggttct ggtgaatttt
      781 aaagacaagt atccttcatc atttgaataa gctatccact caataatgtt aagatctaac
      841 tcttcagggg agtttttagc agtaaagAgc aggttaagct ctttagctaa agcaatgata
      901 tcatcccctc ttagcgaaat tttgctggca ccaagtaatt taccaccctc ttttaattgt
      961 cgataagcaa gcgatggggt gattttggcg agcgctgcaa gatcttcagc cctaattttg
     1021 aaaactcgcc ctcgttcaag aggttctttg ctatcaatgg gagcaagcgc catatacatc
     1081 actcgctttg cgggcaaagg aagttgagcc agggtgtgat ttagctcgtt tcggtggcga
     1141 attttcgttt ttttgttcac gtccatcatg accttgagtc tCATTCTAGT ATTTCCCCTC
     1201 TTTCTCTAGT Attaaacaaa attatttgta gaggctgttt cgtcctcacg gactcatcag
     1261 accggaaagc acatccggtg acagctAGTA GTGCTCAGTA TCTCTATCAC TGATAGGGAT
     1321 GTCAATCTCT ATCACTGATA GGGAAGTAtt gagaagagaa aagaaaaccg ccgatcctgt
     1381 ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt tggctgaaAC
     1441 CTtcagccaa acgtctcttc aggccactga ctagcgataa ctttccccac aacggaacaa
     1501 ctctcattgc atgggatcat tgggtactgt gggtttagtg gttgtaaaaa cacctgaccg
     1561 ctatccctga tcagtttctt gaaggtaaac tcatcacccc caagtctggc tatgcagaaa
     1621 tcacctggct caacagcctg ctcagggtca acgagaatta acattccgtc aggaaagctt
     1681 ggcttggagc ctgttggtgc ggtcatggaa ttaccttcaa cctcaagcca gaatgcagaa
     1741 tcactggctt ttttggttgt gcttacccat ctctccgcat cacctttggt aaaggttcta
     1801 agctcaggtg agaacatccc tgcctgaaca tgagaaaaaa cagggtactc atactcactt
     1861 ctaagtgacg gctgcatact aaccgcttca tacatctcgt agatttctct ggcgattgaa
     1921 gggctaaatt cttcaacgct aactttgaga atttttgcaa gcaatgcggc gttataagca
     1981 tttaatgcat tgatgccatt aaataaagca ccaacgcctg actgccccat ccccatcttg
     2041 tctgcgacag attcctggga taagccaagt tcatttttct ttttttcata aattgcttta
     2101 aggcgacgtg cgtcctcaag ctgctcttgt gttaatggtt tcttttttgt gctCATTggt
     2161 acctttctcc tctttaatga attcAGTAgc tagcataata cctaggactg agctagctgt
     2221 aaaCTCCGAA GTTCCTATTC TTTCTTGAGA ATAGGAACTT CTTGCCTTTT CCGCTGCATA
     2281 cattcgatgg tgtcaacgta aatgcatgcc gcttcgcctt ccggccacca gaatagcctg
     2341 cgattcaacc ccttcttcga tctgttttgc tacccgttgt agcgccggaa gatgcttttc
     2401 cgctgcctgt tcaatggtca ttgcgctcgc catatacacc agattcagac agccaatcac
     2461 ccgttgttca
//