LOCUS       Exported               11214 bp ds-DNA     circular SYN 16-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    FUGW
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   S-Lab
  TITLE     Direct Submission
  JOURNAL   Exported Oct 16, 2025 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11214
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        239..815
                     /label=CMV_immearly_promoter
     enhancer        318..605
                     /label=CAG_enhancer
     LTR             835..1015
                     /label=HIV-1_5_LTR
     misc_feature    1126..1170
                     /label=HIV-1_psi_pack
     misc_feature    1680..1913
                     /label=RRE
     misc_feature    2444..2459
                     /label=cPPT
     promoter        2618..3846
                     /label=hUbC_promoter
     misc_feature    3875..3880
                     /label=Fusion site of BamHI and BglII
     misc_feature    3881..3889
                     /label=Kozak+Start
     misc_feature    3890..3940
                     /label=N-term
     CDS             3908..3910
                     /codon_start=1
                     /label=C8:palmitoylation
                     /translation="C"
     CDS             3911..3913
                     /codon_start=1
                     /label=C9:palmitoylation
                     /translation="C"
     misc_feature    3941..3952
                     /label=R V E P
     misc_feature    3953..4153
                     /label=ProlineRichDomain
     CDS             4160..4876
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    4883..5839
                     /label=TurboID
     misc_feature    5846..5872
                     /label=NES
     misc_feature    5876..5881
                     /label=STOP CONDON
     misc_feature    5882..5887
                     /label=Fusion site of EcoRI and MfeI
     misc_feature    5908..6495
                     /label=WPRE
     misc_feature    6682..6703
                     /label=cPPT/U3PPT
     LTR             7019..7199
                     /label=HIV-1_5_LTR
     polyA_signal    7228..7455
                     /label=bGH_PA_terminator
     rep_origin      7518..7824
                     /label=f1_origin
     enhancer        complement(7944..8159)
                     /label=SV40-Enhancer
     promoter        7956..8224
                     /label=SV40_promoter
     rep_origin      8123..8200
                     /label=SV40_origin
     promoter        8318..8385
                     /label=EM7_promoter
     misc_feature    8386..8760
                     /label=sh_ble
     polyA_site      8893..9012
                     /label=SV40_PA_terminator
     promoter        complement(9109..9138)
                     /label=Lac Promoter
     rep_origin      complement(9447..10066)
                     /direction=LEFT
                     /label=pBR322 origin
     misc_feature    complement(10221..11081)
                     /label=Ampicillin R
     promoter        complement(11123..11151)
                     /label=AmpR-Promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg
     2641 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc aggagcgttc
     2701 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
     2761 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
     2821 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
     2881 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
     2941 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
     3001 gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
     3061 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
     3121 gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga
     3181 atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     3241 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     3301 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     3361 gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt
     3421 acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     3481 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
     3541 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     3601 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     3661 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     3721 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaag
     3781 cttctgcagg tcgactctag aaaattgtcc gctaaattct ggccgttttt ggcttttttg
     3841 ttagacgaag cttgggctgc aggtcgactc tagaggatct gccaccatgt tcagtctgat
     3901 ggccaactgc tgcaacttgt tcaagcggtg gcgggagcct cgggtagaac cagtcaatac
     3961 agacgagtct actccaaaga gtcccacgcc tccccaacct ccccctcctg ttggctgggg
     4021 aacccccaaa gtcactagac ttccaaaact tgagcctctt ggtgaaacac gtcacaatga
     4081 tttctatggg aagccgctgc ctcccctggc tgtgcgacag agacctaacg gtgatgctca
     4141 ggacacgatc tcaggatcca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     4201 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     4261 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     4321 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     4381 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     4441 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     4501 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     4561 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     4621 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     4681 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     4741 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     4801 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     4861 ggacgagctg tacaaggcta gcaaagacaa tactgtgcct ctgaagctga tcgctctcct
     4921 ggctaatggc gagttccata gtggcgaaca gctgggagaa accctgggca tgtccagggc
     4981 cgctatcaac aagcacattc agactctgcg cgactggggc gtggacgtgt tcaccgtgcc
     5041 cggaaagggc tactctctgc ccgagcctat cccgctgctg aacgctaaac agattctggg
     5101 acagctggac ggcgggagcg tggcagtcct gcctgtggtc gactccacca atcagtacct
     5161 gctggatcga atcggcgagc tgaagagtgg ggatgcttgc attgcagaat atcagcaggc
     5221 agggagagga agcagaggga ggaaatggtt ctctcctttt ggagctaacc tgtacctgag
     5281 tatgttttgg cgcctgaagc ggggaccagc agcaatcggc ctgggcccgg tcatcggaat
     5341 tgtcatggca gaagcgctgc gaaagctggg agcagacaag gtgcgagtca aatggcccaa
     5401 tgacctgtat ctgcaggata gaaagctggc aggcatcctg gtggagctgg ccggaataac
     5461 aggcgatgct gcacagatcg tcattggcgc cgggattaac gtggctatga ggcgcgtgga
     5521 ggaaagcgtg gtcaatcagg gctggatcac actgcaggaa gcagggatta acctggacag
     5581 gaatactctg gccgctacgc tgatccgaga gctgcgggca gccctggaac tgttcgagca
     5641 ggaaggcctg gctccatatc tgccacggtg ggagaagctg gataacttca tcaatagacc
     5701 cgtgaagctg atcattgggg acaaagagat tttcgggatt agccggggga ttgataaaca
     5761 gggagccctg ctgctggaac aggacggagt tatcaaaccc tggatgggcg gagaaatcag
     5821 tctgcggtct gccgaaaagc tgcagctgcc tcccctggag cgcctgaccc tggactaata
     5881 gcaattcgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     5941 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     6001 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6061 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6121 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6181 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6241 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6301 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6361 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6421 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6481 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     6541 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     6601 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     6661 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6721 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     6781 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     6841 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     6901 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     6961 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     7021 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7081 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7141 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7201 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7261 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7321 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7381 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     7441 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     7501 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7561 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7621 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7681 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7741 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7801 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7861 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7921 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7981 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     8041 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8101 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     8161 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     8221 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     8281 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     8341 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     8401 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     8461 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     8521 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     8581 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     8641 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     8701 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     8761 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     8821 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     8881 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8941 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9001 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     9061 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9121 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9181 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9241 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9301 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9361 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     9421 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     9481 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     9541 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     9601 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     9661 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     9721 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     9781 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     9841 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     9901 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     9961 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10021 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10081 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10141 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10201 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10261 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10321 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10381 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    10441 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    10501 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    10561 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    10621 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    10681 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    10741 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    10801 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    10861 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    10921 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    10981 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11041 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11101 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11161 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac
//
