LOCUS       Exported               11151 bp ds-DNA     circular SYN 16-OCT-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    FUGW
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11151)
  AUTHORS   S-Lab
  TITLE     Direct Submission
  JOURNAL   Exported Oct 16, 2025 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11151
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        239..815
                     /label=CMV_immearly_promoter
     enhancer        318..605
                     /label=CAG_enhancer
     LTR             835..1015
                     /label=HIV-1_5_LTR
     misc_feature    1126..1170
                     /label=HIV-1_psi_pack
     misc_feature    1680..1913
                     /label=RRE
     misc_feature    2444..2459
                     /label=cPPT
     promoter        2618..3846
                     /label=hUbC_promoter
     misc_feature    3875..3880
                     /label=Fusion site of BamHI and BglII
     misc_feature    3881..3889
                     /label=Kozak+Start
     misc_feature    3890..4090
                     /label=ProlineRichDomain
     CDS             4097..4813
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    4820..5776
                     /label=TurboID
     misc_feature    5783..5809
                     /label=NES
     misc_feature    5813..5818
                     /label=STOP CONDON
     misc_feature    5819..5824
                     /label=Fusion site of EcoRI and MfeI
     misc_feature    5845..6432
                     /label=WPRE
     misc_feature    6619..6640
                     /label=cPPT/U3PPT
     LTR             6956..7136
                     /label=HIV-1_5_LTR
     polyA_signal    7165..7392
                     /label=bGH_PA_terminator
     rep_origin      7455..7761
                     /label=f1_origin
     enhancer        complement(7881..8096)
                     /label=SV40-Enhancer
     promoter        7893..8161
                     /label=SV40_promoter
     rep_origin      8060..8137
                     /label=SV40_origin
     promoter        8255..8322
                     /label=EM7_promoter
     misc_feature    8323..8697
                     /label=sh_ble
     polyA_site      8830..8949
                     /label=SV40_PA_terminator
     promoter        complement(9046..9075)
                     /label=Lac Promoter
     rep_origin      complement(9384..10003)
                     /direction=LEFT
                     /label=pBR322 origin
     misc_feature    complement(10158..11018)
                     /label=Ampicillin R
     promoter        complement(11060..11088)
                     /label=AmpR-Promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg
     2641 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc aggagcgttc
     2701 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
     2761 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
     2821 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
     2881 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
     2941 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
     3001 gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
     3061 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
     3121 gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga
     3181 atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     3241 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     3301 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     3361 gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt
     3421 acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     3481 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
     3541 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     3601 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     3661 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     3721 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaag
     3781 cttctgcagg tcgactctag aaaattgtcc gctaaattct ggccgttttt ggcttttttg
     3841 ttagacgaag cttgggctgc aggtcgactc tagaggatct gccaccatgg tcaatacaga
     3901 cgagtctact ccaaagagtc ccacgcctcc ccaacctccc cctcctgttg gctggggaac
     3961 ccccaaagtc actagacttc caaaacttga gcctcttggt gaaacacgtc acaatgattt
     4021 ctatgggaag ccgctgcctc ccctggctgt gcgacagaga cctaacggtg atgctcagga
     4081 cacgatctca ggatccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     4141 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     4201 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     4261 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     4321 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     4381 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     4441 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     4501 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     4561 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     4621 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     4681 gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa
     4741 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     4801 cgagctgtac aaggctagca aagacaatac tgtgcctctg aagctgatcg ctctcctggc
     4861 taatggcgag ttccatagtg gcgaacagct gggagaaacc ctgggcatgt ccagggccgc
     4921 tatcaacaag cacattcaga ctctgcgcga ctggggcgtg gacgtgttca ccgtgcccgg
     4981 aaagggctac tctctgcccg agcctatccc gctgctgaac gctaaacaga ttctgggaca
     5041 gctggacggc gggagcgtgg cagtcctgcc tgtggtcgac tccaccaatc agtacctgct
     5101 ggatcgaatc ggcgagctga agagtgggga tgcttgcatt gcagaatatc agcaggcagg
     5161 gagaggaagc agagggagga aatggttctc tccttttgga gctaacctgt acctgagtat
     5221 gttttggcgc ctgaagcggg gaccagcagc aatcggcctg ggcccggtca tcggaattgt
     5281 catggcagaa gcgctgcgaa agctgggagc agacaaggtg cgagtcaaat ggcccaatga
     5341 cctgtatctg caggatagaa agctggcagg catcctggtg gagctggccg gaataacagg
     5401 cgatgctgca cagatcgtca ttggcgccgg gattaacgtg gctatgaggc gcgtggagga
     5461 aagcgtggtc aatcagggct ggatcacact gcaggaagca gggattaacc tggacaggaa
     5521 tactctggcc gctacgctga tccgagagct gcgggcagcc ctggaactgt tcgagcagga
     5581 aggcctggct ccatatctgc cacggtggga gaagctggat aacttcatca atagacccgt
     5641 gaagctgatc attggggaca aagagatttt cgggattagc cgggggattg ataaacaggg
     5701 agccctgctg ctggaacagg acggagttat caaaccctgg atgggcggag aaatcagtct
     5761 gcggtctgcc gaaaagctgc agctgcctcc cctggagcgc ctgaccctgg actaatagca
     5821 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     5881 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     5941 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6001 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6061 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6121 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6181 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     6241 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6301 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6361 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6421 cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
     6481 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     6541 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     6601 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     6661 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     6721 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     6781 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     6841 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     6901 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     6961 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7021 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     7081 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     7141 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     7201 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     7261 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     7321 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     7381 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     7441 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     7501 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     7561 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     7621 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     7681 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     7741 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     7801 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     7861 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     7921 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     7981 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     8041 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     8101 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     8161 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     8221 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     8281 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     8341 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     8401 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     8461 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     8521 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     8581 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     8641 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     8701 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     8761 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     8821 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8881 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8941 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     9001 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     9061 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     9121 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     9181 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     9241 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     9301 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     9361 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     9421 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     9481 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     9541 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     9601 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     9661 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     9721 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     9781 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     9841 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     9901 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     9961 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10021 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    10081 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    10141 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    10201 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    10261 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    10321 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    10381 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    10441 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    10501 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    10561 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    10621 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    10681 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    10741 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    10801 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    10861 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    10921 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    10981 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    11041 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    11101 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga c
//
