LOCUS       pTIR_HyPB_RFP    3519 bp ds-DNA     circular     15-MAY-2025
DEFINITION  .
KEYWORDS    "Backbone" "Resistance" "Strain" "Gene of interest" "Glycerol Stock
            (date)"
FEATURES             Location/Qualifiers
     misc_feature    13..31
                     /label="2.G9_SYBR-Amp_Fw"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ACTCGCCTTGATCGTTGGG"
     primer          13..31
                     /label="SYBR-Amp_Fw"
                     /note="sequence: ACTCGCCTTGATCGTTGGG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(78..98)
                     /label="2.H1_SYBR-Amp_Rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GTTGCCATTGCTACAGGCATC"
     primer          complement(78..98)
                     /label="SYBR-Amp_Rv"
                     /note="sequence: GTTGCCATTGCTACAGGCATC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    404..424
                     /label="6.G2_pT4_2/2_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ATCGCGTCTCACATAtgtcagaccaagtttactcat"
     rep_origin      573..1161
                     /label="ori"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     misc_feature    complement(839..853)
                     /label="14.D4_SINeV2/2AAGG_F"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="sequence: ATCGCGTCTCAcgttgagtccaaccctg"
     misc_feature    933..948
                     /label="2.D8_cas9AgeI R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: gcataccggtgtctccaccgagctgaga"
     misc_feature    933..948
                     /label="1.G9_cas9_R_Esp3I"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GCAGACGTCTCCTCCgtctccaccgagctgaga"
     primer_bind     1062..1081
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="note: pBR322 origin, forward primer"
     primer          1062..1081
                     /label="pBR322ori-FW_PhosThio**"
                     /note="sequence: tacgaCCAATCCTGTCCCTAGTGGCCCCgggaaacgcctggtatcttt"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1062..1081
                     /label="ITX12_E5_pBR322ori-F"
                     /note="sequence: GGGAAACGCCTGGTATCTTT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1062..1081
                     /label="oITX15_D4_pBR322 ori Fw_miseq"
                     /note="sequence: ACACTCTTTCCCTACACGACGCTCTTCCGATCTGGGAAACGCCTGGTATCTTT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1158..1180)
                     /label="17.A9_puc19.BbsI-bb_CGAA.F_II"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="sequence: TCGGAATTCGAAGACTACGAAgccgcgttgctggcgtttttc"
     misc_feature    1180..1202
                     /label="9.G6_cPCR-dbDNA_miseq_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ACACTCTTTCCCTACACGACGCTCTTCCGATCTttacggttcctggccttttg"
     misc_feature    complement(1182..1203)
                     /label="5.E4_puc19_BspqI_mini_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ctcgccgctcttccgctgaattcGCAAAAGGCCAGGAACCGTAAA"
     misc_feature    1183..1201
                     /label="cPCR-dbDNA-F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="sequence: ttacggttcctggcctttt"
     misc_feature    1183..1202
                     /label="ITX5_I6_cPCR-dbDNA-F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="sequence: ttacggttcctggccttttg"
     misc_feature    1183..1202
                     /label="5.I6_cPCR-dbDNA-F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ttacggttcctggccttttg"
     primer          1183..1202
                     /label="ITX5_I6_cPCR-dbDNA-F"
                     /note="sequence: ttacggttcctggccttttg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(1212..1274)
                     /label="5'ITR-hyPB-long"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1225..1255
                     /label="31bp spacer"
                     /ApEinfo_revcolor="#43ffb8"
                     /ApEinfo_fwdcolor="#43ffb8"
     misc_feature    complement(1225..1255)
                     /label="5'TR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1247..1271)
                     /label="ITX_E1_HyPB_5ITR_R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1279..1297
                     /label="ITX12_H5.PB-512_bb_E3I.EcoRI._F_I"
                     /note="sequence: ATCGGAATTCCGTCTCATAACaaggatctgcgatcgctcc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1279..1297
                     /label="FWD_1/2GFPflip_F"
                     /note="sequence: ATCGCGTCTCAGAGCaaggatctgcgatcgctcc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1279..1298)
                     /label="ITX15_A5_EF1_5' junction mapping REV_P5 miseq_FW"
                     /note="sequence: ACACTCTTTCCCTACACGACGCTCTTCCGATCTcggagcgatcgcagatcctt"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1279..1299
                     /label="AAVS1gRNA_EF1a_Fw"
                     /note="sequence: tacgaCCAATCCTGTCCCTAGTGGCCCCaaggatctgcgatcgctccgg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1279..1299
                     /label="TRACgRNA_EF1a_Fw"
                     /note="sequence: tacgaACCTCATGTCTAGCACAGTTTTGTaaggatctgcgatcgctccgg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    1279..1824
                     /label="EF1 promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1310..1347
                     /label="BspQ1-TET-on-Rv"
                     /note="sequence: ATCGaagcttgctcttccCATgggcagagcgcacatcgcccacagtccccgagaagtt"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1636..1655
                     /label="EcoRI_pRRL_Ef1a_F"
                     /note="sequence: ATCGGAATTCCGTAactgcgtccgccgtctagg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    1798..1855
                     /label="01.A6_P2A.RFP_E3I.AGGA_F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="sequence: ATCGCGTCTCAaggagatgttgaagagaatcctggtcccatggccagctccgaggatg"
     misc_feature    complement(1806..1824)
                     /label="EF1 promoter rv"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1806..1824)
                     /label="ITX12_E3_EF1 promoter rv"
                     /note="sequence: gtaggcgccggtcacagct"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(1811..1851)
                     /label="14.G7_Ef1a.Esp3I.EcoRI_R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="sequence: tgcaggaattcgcagCGAGACGATATCGTAGGCGCCGGTCA"
     misc_feature    complement(1811..1868)
                     /label="14.G9_Ef1a.Esp3I.Esp3I.EcoRI_R"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="sequence: tgcaggaattcgcagCGAGACGtgctgaCGTCTCGgggcATATCGTAGGCGCCGGTCA"
     misc_feature    1823..1855
                     /label="01.A5_RFP_E3I.AAAT_F"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: TCGCGTCTCCAAATatggccagctccgaggatg"
     primer          1830..1855
                     /label="pSico-RFP-fw"
                     /note="sequence: ATCGaagcttCGTCTCAGCCCGCCACCATGgccagctccgaggatg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1837..1855
                     /label="SYBR-RFP-Fw"
                     /note="sequence: atggccagctccgaggatg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    1837..2514
                     /label="mRFP"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    1845..1848
                     /label="GGov"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    1905..1924
                     /label="gRNA1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2010..2031)
                     /label="SYBR-RFP-Rv"
                     /note="sequence: GAACTGAGGGCTCAGAATATCC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2445..2467
                     /label="oITX15_A2_3' RFP_P5 miseq_RV"
                     /note="sequence: ACTGGAGTTCAGACGTGTGCTCTTCCGATCTCcataatggggactacaccatcg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2446..2467
                     /label="ITX14_H9_RFP_3'_REV"
                     /note="sequence: cataatggggactacaccatcg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2446..2467
                     /label="oITX15_A1_3' RFP_P5_miseq_FW"
                     /note="sequence: ACACTCTTTCCCTACACGACGCTCTTCCGATCTcataatggggactacaccatcg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2505..2523)
                     /label="pSico-RFP-rv"
                     /note="sequence: ATCGaagcttCGTCTCAGCAGgtcgacttatccagcgcct"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2512..2536
                     /label="MC-SalI-ITR3-Fw"
                     /note="sequence: ggataagtcgacaatcaacctctgg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2517..2537
                     /label="SalI-WPRE-BGH-Fw"
                     /note="sequence: gtaccagtcgacaatcaacctctgga"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2524..2564
                     /label="WPRE_R"
                     /note="sequence: TGGATCCACCTGCCAGTTATCATGTCTGaatcaacctctggattacaaaatttgtgaaagattgactgg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2577..2597)
                     /label="ITX14_G2_WPRE_Rv"
                     /note="sequence: catagcgtaaaaggagcaaca"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2639..2663
                     /label="SV40pA_R"
                     /note="sequence: TGGATCCACCTGCTCACAAACTTGTTTATTGCAGCTTATAATG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2639..2769
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2675..2699
                     /label="ITX12_E8_SV40pA fw"
                     /note="sequence: AGCAATAGCATCACAAATTTCACAA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(2676..2695)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2676..2695)
                     /label="ITX12_D9_SV40pA-R"
                     /note="sequence: gaaatttgtgatgctattgc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2727..2753
                     /label="oITX15_A3_3' RFP_P5_miseq_FW#2"
                     /note="sequence: ACACTCTTTCCCTACACGACGCTCTTCCGATCTGTTGTGGTTTGTCCAAACTCATCAATG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2770..2797
                     /label="Orth-3p_GSP1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          2770..2797
                     /label="ITX3_E1_Orth-3p_GSP1"
                     /note="sequence: GAATTGACTCAAATGATGTCAATTAGTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2801..2822
                     /label="oITX1120_ITX14_I9_new_3' RFP_R_P5 miseq_FW"
                     /note="sequence: ACTGGAGTTCAGACGTGTGCTCTTCCGATCTCCAGAAGCTATCTGGTCTCCCTT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2801..2822)
                     /label="12.G2_SV40pA_TAAC_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ATCGCGTCTCAGTTAAAGGGAGACCAGATAGCTTCTG"
     misc_feature    complement(2801..2822)
                     /label="seq SV40pA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2801..2822)
                     /label="ITX12_E1_seq SV40pA"
                     /note="sequence: AAGGGAGACCAGATAGCTTCTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2817..2837
                     /label="IT5_E2_dPCR_HyPB_3ITR_R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2817..2837
                     /label="hyPB-A_GSP2"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          2817..2837
                     /label="ITX3_E3_hyPB-A_GSP2"
                     /note="sequence: CAGACGTGTGCTCTTCCGATCTTCCCTTGAGCCATGCGTCAAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(2827..2861)
                     /label="3'ITR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          complement(2834..2865)
                     /label="ITX16_F9_3'ITR_Rosa26_HITI"
                     /note="sequence: gctagcCCCCGGAGTGCGCGATGGAGGTCTTAACCCTAGAAAGATAGTCTGCGTAAAATTG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(2842..2861)
                     /label="PB-Luci_dd_R"
                     /note="sequence: ccctagaaagatagtctgcg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2846..2848
                     /label="3 bp spacer"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(2870..2888)
                     /label="ITX12_D6_PAM-library-Fw"
                     /note="sequence: AATAGGCGTATCACGAGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2870..2888)
                     /label="ITX1_B5_PAM-lib-Rv_Phos**"
                     /note="sequence: AATAGGCGTATCACGAGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2881..2912
                     /label="ITX4_G7_Orth_3p_blocking"
                     /note="sequence: CGCCTATTTTTATAGGTTAATGTCATGATAAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2907..2931)
                     /label="ITX16_B2_3ITR-ORTH-TTAA-Rv"
                     /note="sequence: tgacgtctaagaaaccattattatc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(2933..2951)
                     /label="5.I7_cPCR-dbDNA-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: tttccccgaaaagtgccac"
     misc_feature    complement(2933..2951)
                     /label="ITX5_I7_cPCR-dbDNA-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: tttccccgaaaagtgccac"
     primer          complement(2933..2951)
                     /label="cPCR-dbDNA-R"
                     /note="sequence: tttccccgaaaagtgccac"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    complement(2933..2953)
                     /label="9.G7_cPCR-dbDNA_miseq_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ACTGGAGTTCAGACGTGTGCTCTTCCGATCTtttccccgaaaagtgccac"
     misc_feature    2933..2954
                     /label="5.E3_puc19_BspqI_mini_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: cgaggagctcttccatgaattcGTGGCACTTTTCGGGGAAATGT"
     primer          2934..2957
                     /label="ITX15_A7_Amp_FW_excision test"
                     /note="sequence: tggcacttttcggggaaatgtgcg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2940..2959)
                     /label="4.D1_Fw_prim_tolC_int_sw102"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: CGCGCACATTTCCCCGAAAA"
     primer          complement(2940..2959)
                     /label="Fw_prim_tolC_int_sw102"
                     /note="sequence: CGCGCACATTTCCCCGAAAA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     promoter        2956..3060
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="gene: bla"
     misc_feature    complement(3159..3175)
                     /label="3.G5_5116CT_SortTem-Rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: cacACCAACTCATCTTCAGCA"
     misc_feature    complement(3159..3175)
                     /label="1.F1_Hs-Lama-Cr-5116rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GACTGGAGTTCAGACGTGTGCTCTTCCGATCTCACACCAACTCATCTTCAGCA"
     misc_feature    complement(3159..3175)
                     /label="2.C3_Lama2-Sang-5116-Rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: CACACCAACTCATCTTCAGCA"
     primer_bind     complement(3279..3298)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: Ampicillin resistance gene, reverse primer"
     misc_feature    complement(3279..3298)
                     /label="ITX10-F6_Amp-Rv"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="sequence: ATAATACCGCGCCACATAGC"
     primer          complement(3279..3298)
                     /label="oITX10_F6_Amp-Rv"
                     /note="sequence: ataataccgcgccacatagc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(3279..3298)
                     /label="Amp-Rv_PhosThio**"
                     /note="sequence: tacgaCCAATCCTGTCCCTAGTGGCCCCataataccgcgccacatagc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(3279..3298)
                     /label="oITX15_D5_Amp-R_miseq"
                     /note="sequence: ACTGGAGTTCAGACGTGTGCTCTTCCGATCTCataataccgcgccacatagc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
ORIGIN
        1 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
       61 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
      121 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
      181 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
      241 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
      301 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
      361 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
      421 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
      481 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
      541 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
      601 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
      661 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
      721 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
      781 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
      841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
      901 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
      961 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1021 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1081 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1141 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1201 tgcTGCCTTA ACCCTAGAAA GATAATCATA TTGTGACGTA CGTTAAAGAT AATCATGCGT
     1261 AAAATTGACG CAtGTATGaa ggatctgcga tcgctccggt gcccgtcagt gggcagagcg
     1321 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa cgggtgccta
     1381 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     1441 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     1501 cgggtttgcc gccagaacac agctgaagct tcgaggggct cgcatctctc cttcacgcgc
     1561 ccgccgccct acctgaggcc gccatccacg ccggttgagt cgcgttctgc cgcctcccgc
     1621 ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt ttaaagctca ggtcgagacc
     1681 gggcctttgt ccggcgctcc cttggagcct acctagactc agccggctct ccacgctttg
     1741 cctgaccctg cttgctcaac tctacgtctt tgtttcgttt tctgttctgc gccgttacag
     1801 atccaagctg tgaccggcgc ctacgatatc gccaccatgg ccagctccga ggatgtcatc
     1861 aaagagttta tgagatttaa ggtcaagatg gagggaagcg tcaacggaca cgagttcgag
     1921 attgagggag aaggagaagg ccggccttac gagggcacac aaaccgctaa gctcaaggtc
     1981 acaaaaggag gacccctccc cttctcctgg gatattctga gccctcagtt ccagtacgga
     2041 agcaaagcct atgttaaaca ccctgccgac atccctgact atctgaagct ctccttccct
     2101 gaaggcttca agtgggagag attcatgaac ttcgaggacg gaggcgtggt gacagtcaca
     2161 caagatagca ccctccagga cggagagttt atttataagg tgaaactcag aggaaccaac
     2221 ttcccctccg atggccctgt catgcaaaaa aaaacaatgg gatgggaagc ctccaccgag
     2281 agaatgtatc ctgaggatgg cgctctgaaa ggcgaaatta aaatgagact gaaactcaaa
     2341 gacggaggac actacgatgc cgaggtcaaa acaacctaca aggccaagaa acaagtgcag
     2401 ctgcctggcg cctacatgac tgatattaaa ctcgacatta tcagccataa tggggactac
     2461 accatcgtgg aacaatatga gagagctgag ggcagacata gcacaggcgc tggataagtc
     2521 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2581 gctcctttta cgctatgtgG ATACGCTGCT TTAATGCCTT TGTATCATGC GTTAACTAAA
     2641 CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA
     2701 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA
     2761 TCATGTCTGG AATTGACTCA AATGATGTCA ATTAGTCTAT CAGAAGCTAT CTGGTCTCCC
     2821 TTGAGCCATG CGTCAATTTT ACGCAGACTA TCTTTCTAGG GTTAAAGGAG CCTCGTGATA
     2881 CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAgacgtc aggtggcact
     2941 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     3001 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     3061 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     3121 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     3181 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     3241 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     3301 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     3361 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     3421 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     3481 ggaggaccga aggagctaac cgcttttttg cacaacatg
//