LOCUS       pTIR_Poetur_RFP         3711 bp ds-DNA     circular     24-OCT-2025
DEFINITION  .
KEYWORDS    "Backbone" "Gene of interest" "Glycerol Stock (date)" "Resistance"
            "Strain"
COMMENT     Imported using the Genbank importer. File name: pTIR_Poetur_RFP.gb
FEATURES             Location/Qualifiers
     primer          108..126
                     /label="SYBR-Amp_Fw"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ACTCGCCTTGATCGTTGGG"
     misc_feature    108..126
                     /label="2.G9_SYBR-Amp_Fw"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ACTCGCCTTGATCGTTGGG"
     primer          complement(173..193)
                     /label="SYBR-Amp_Rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GTTGCCATTGCTACAGGCATC"
     misc_feature    complement(173..193)
                     /label="2.H1_SYBR-Amp_Rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GTTGCCATTGCTACAGGCATC"
     misc_feature    499..519
                     /label="6.G2_pT4_2/2_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ATCGCGTCTCACATAtgtcagaccaagtttactcat"
     rep_origin      668..1256
                     /label="ori"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     misc_feature    complement(934..948)
                     /label="14.D4_SINeV2/2AAGG_F"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="sequence: ATCGCGTCTCAcgttgagtccaaccctg"
     misc_feature    1028..1043
                     /label="1.G9_cas9_R_Esp3I"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GCAGACGTCTCCTCCgtctccaccgagctgaga"
     misc_feature    1028..1043
                     /label="2.D8_cas9AgeI R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: gcataccggtgtctccaccgagctgaga"
     primer          1157..1176
                     /label="ITX12_E5_pBR322ori-F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GGGAAACGCCTGGTATCTTT"
     primer_bind     1157..1176
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="note: pBR322 origin, forward primer"
     primer          1157..1176
                     /label="pBR322ori-FW_PhosThio**"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="sequence: tacgaCCAATCCTGTCCCTAGTGGCCCCgggaaacgcctggtatcttt"
     primer          1157..1176
                     /label="oITX15_D4_pBR322 ori Fw_miseq"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ACACTCTTTCCCTACACGACGCTCTTCCGATCTGGGAAACGCCTGGTATCTTT"
     misc_feature    complement(1253..1275)
                     /label="17.A9_puc19.BbsI-bb_CGAA.F_II"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="sequence: TCGGAATTCGAAGACTACGAAgccgcgttgctggcgtttttc"
     misc_feature    1275..1297
                     /label="9.G6_cPCR-dbDNA_miseq_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ACACTCTTTCCCTACACGACGCTCTTCCGATCTttacggttcctggccttttg"
     misc_feature    complement(1277..1298)
                     /label="5.E4_puc19_BspqI_mini_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ctcgccgctcttccgctgaattcGCAAAAGGCCAGGAACCGTAAA"
     misc_feature    1278..1296
                     /label="cPCR-dbDNA-F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="sequence: ttacggttcctggcctttt"
     misc_feature    1278..1297
                     /label="ITX5_I6_cPCR-dbDNA-F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /note="sequence: ttacggttcctggccttttg"
     misc_feature    1278..1297
                     /label="5.I6_cPCR-dbDNA-F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ttacggttcctggccttttg"
     primer          1278..1297
                     /label="ITX5_I6_cPCR-dbDNA-F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ttacggttcctggccttttg"
     misc_feature    1303..1452
                     /label="ITR5´"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1319..1339)
                     /label="IT5_D2_dPCR_POE_5ITR_R2"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(1319..1339)
                     /label="ITX5_D2_dPCR_POE_5ITR_R2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: CGGGTCAAATTGACCCGAAAC"
     primer          complement(1319..1341)
                     /label="oITX15_H1_Poe_Turru_trim_ITR_RV"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GAATTCCGTCTCACATAACGGGTCAAATTGACCCGAAAC"
     misc_feature    complement(1349..1371)
                     /label="ITX5_D1_dPCR_POE_5ITR_R"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(1349..1371)
                     /label="ITX5_D1_dPCR_POE_5ITR_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: AACCTGCACTTTGGTCAACTCAA"
     primer          1457..1475
                     /label="ITX12_H5.PB-512_bb_E3I.EcoRI._F_I"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ATCGGAATTCCGTCTCATAACaaggatctgcgatcgctcc"
     primer          1457..1475
                     /label="FWD_1/2GFPflip_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ATCGCGTCTCAGAGCaaggatctgcgatcgctcc"
     primer          complement(1457..1476)
                     /label="ITX15_A5_EF1_5' junction mapping REV_P5 miseq_FW"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ACACTCTTTCCCTACACGACGCTCTTCCGATCTcggagcgatcgcagatcctt"
     primer          1457..1477
                     /label="TRACgRNA_EF1a_Fw"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: tacgaACCTCATGTCTAGCACAGTTTTGTaaggatctgcgatcgctccgg"
     primer          1457..1477
                     /label="AAVS1gRNA_EF1a_Fw"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: tacgaCCAATCCTGTCCCTAGTGGCCCCaaggatctgcgatcgctccgg"
     misc_feature    1457..2002
                     /label="EF1 promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1488..1525
                     /label="BspQ1-TET-on-Rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ATCGaagcttgctcttccCATgggcagagcgcacatcgcccacagtccccgagaagtt"
     primer          1814..1833
                     /label="EcoRI_pRRL_Ef1a_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ATCGGAATTCCGTAactgcgtccgccgtctagg"
     misc_feature    1976..2033
                     /label="01.A6_P2A.RFP_E3I.AGGA_F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="sequence: ATCGCGTCTCAaggagatgttgaagagaatcctggtcccatggccagctccgaggatg"
     misc_feature    complement(1984..2002)
                     /label="EF1 promoter rv"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(1984..2002)
                     /label="ITX12_E3_EF1 promoter rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: gtaggcgccggtcacagct"
     misc_feature    complement(1989..2029)
                     /label="14.G7_Ef1a.Esp3I.EcoRI_R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="sequence: tgcaggaattcgcagCGAGACGATATCGTAGGCGCCGGTCA"
     misc_feature    complement(1989..2046)
                     /label="14.G9_Ef1a.Esp3I.Esp3I.EcoRI_R"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="sequence: tgcaggaattcgcagCGAGACGtgctgaCGTCTCGgggcATATCGTAGGCGCCGGTCA"
     misc_feature    2001..2033
                     /label="01.A5_RFP_E3I.AAAT_F"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /note="sequence: TCGCGTCTCCAAATatggccagctccgaggatg"
     primer          2008..2033
                     /label="pSico-RFP-fw"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ATCGaagcttCGTCTCAGCCCGCCACCATGgccagctccgaggatg"
     primer          2015..2033
                     /label="SYBR-RFP-Fw"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: atggccagctccgaggatg"
     misc_feature    2015..2692
                     /label="mRFP"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    2023..2026
                     /label="GGov"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2083..2102
                     /label="gRNA1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2188..2209)
                     /label="SYBR-RFP-Rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GAACTGAGGGCTCAGAATATCC"
     primer          2623..2645
                     /label="oITX15_A2_3' RFP_P5 miseq_RV"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ACTGGAGTTCAGACGTGTGCTCTTCCGATCTCcataatggggactacaccatcg"
     primer          2624..2645
                     /label="oITX15_A1_3' RFP_P5_miseq_FW"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ACACTCTTTCCCTACACGACGCTCTTCCGATCTcataatggggactacaccatcg"
     primer          2624..2645
                     /label="ITX14_H9_RFP_3'_REV"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: cataatggggactacaccatcg"
     primer          complement(2683..2701)
                     /label="pSico-RFP-rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ATCGaagcttCGTCTCAGCAGgtcgacttatccagcgcct"
     primer          2689..2713
                     /label="MC-SalI-ITR3-Fw"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ggataagtcgacaatcaacctctgg"
     primer          2694..2714
                     /label="SalI-WPRE-BGH-Fw"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: gtaccagtcgacaatcaacctctgga"
     primer          2701..2741
                     /label="WPRE_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: TGGATCCACCTGCCAGTTATCATGTCTGaatcaacctctggattacaaaatttgtgaaagattgactgg"
     primer          complement(2754..2774)
                     /label="ITX14_G2_WPRE_Rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: catagcgtaaaaggagcaaca"
     primer          2815..2840
                     /label="SV40pA_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: TGGATCCACCTGCTCACAAACTTGTTTATTGCAGCTTATAATG"
     primer          2816..2840
                     /label="SV40pA_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: TGGATCCACCTGCTCACAAACTTGTTTATTGCAGCTTATAATG"
     misc_feature    2816..2946
                     /label="SV40 pA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2852..2876
                     /label="ITX12_E8_SV40pA fw"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: AGCAATAGCATCACAAATTTCACAA"
     misc_feature    complement(2853..2872)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2853..2872)
                     /label="ITX12_D9_SV40pA-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: gaaatttgtgatgctattgc"
     primer          2904..2930
                     /label="oITX15_A3_3' RFP_P5_miseq_FW#2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ACACTCTTTCCCTACACGACGCTCTTCCGATCTGTTGTGGTTTGTCCAAACTCATCAATG"
     primer          2947..2974
                     /label="ITX3_E1_Orth-3p_GSP1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GAATTGACTCAAATGATGTCAATTAGTC"
     misc_feature    2953..2974
                     /label="GSP1?"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2978..2999
                     /label="oITX1120_ITX14_I9_new_3' RFP_R_P5 miseq_FW"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ACTGGAGTTCAGACGTGTGCTCTTCCGATCTCCAGAAGCTATCTGGTCTCCCTT"
     primer          complement(2978..2999)
                     /label="ITX12_E1_seq SV40pA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: AAGGGAGACCAGATAGCTTCTG"
     misc_feature    complement(2978..2999)
                     /label="seq SV40pA"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(2978..2999)
                     /label="12.G2_SV40pA_TAAC_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ATCGCGTCTCAGTTAAAGGGAGACCAGATAGCTTCTG"
     misc_feature    3002..3025
                     /label="GSP2?"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3004..3152
                     /label="ITR3´"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3063..3086
                     /label="GSP2-91??"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3087..3110
                     /label="GSP2_115"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          3088..3108
                     /label="oITX15_G9_Poe_Turru_trim_ITR_FW"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GAATTCCGTCTCAGAGCATACGGGTCAAAATGACCCGC"
     primer          3089..3111
                     /label="oITX15_I3_Sim_dia_trim_ITR_FW"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GAATTCCGTCTCAGAGCTACGGGTCAAAATGACCCGCAAC"
     primer          3092..3114
                     /label="oITX15_I4_Sim_dia_trim_ITR_RV"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GAATTCCGTCTCACATAGGGTCAAAATGACCCGCAACATC"
     misc_feature    3094..3116
                     /label="ITX5_D3_dPCR_POE_3ITR_R"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          3094..3116
                     /label="ITX5_D3_dPCR_POE_3ITR_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GTCAAAATGACCCGCAACATCAT"
     primer          complement(3157..3175)
                     /label="ITX12_D6_PAM-library-Fw"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: AATAGGCGTATCACGAGGC"
     primer          complement(3194..3218)
                     /label="ITX16_B2_3ITR-ORTH-TTAA-Rv"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="sequence: tgacgtctaagaaaccattattatc"
     misc_feature    complement(3212..3251)
                     /label="YES"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(3220..3238)
                     /label="ITX5_I7_cPCR-dbDNA-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: tttccccgaaaagtgccac"
     misc_feature    complement(3220..3238)
                     /label="5.I7_cPCR-dbDNA-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: tttccccgaaaagtgccac"
     primer          complement(3220..3238)
                     /label="cPCR-dbDNA-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: tttccccgaaaagtgccac"
     misc_feature    complement(3220..3240)
                     /label="9.G7_cPCR-dbDNA_miseq_R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ACTGGAGTTCAGACGTGTGCTCTTCCGATCTtttccccgaaaagtgccac"
     misc_feature    3220..3241
                     /label="5.E3_puc19_BspqI_mini_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: cgaggagctcttccatgaattcGTGGCACTTTTCGGGGAAATGT"
     primer          3221..3244
                     /label="ITX15_A7_Amp_FW_excision test"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: tggcacttttcggggaaatgtgcg"
     primer          complement(3227..3246)
                     /label="Fw_prim_tolC_int_sw102"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: CGCGCACATTTCCCCGAAAA"
     misc_feature    complement(3227..3246)
                     /label="4.D1_Fw_prim_tolC_int_sw102"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: CGCGCACATTTCCCCGAAAA"
     promoter        3243..3347
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="gene: bla"
     misc_feature    complement(3446..3462)
                     /label="2.C3_Lama2-Sang-5116-Rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: CACACCAACTCATCTTCAGCA"
     misc_feature    complement(3446..3462)
                     /label="3.G5_5116CT_SortTem-Rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: cacACCAACTCATCTTCAGCA"
     misc_feature    complement(3446..3462)
                     /label="1.F1_Hs-Lama-Cr-5116rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: GACTGGAGTTCAGACGTGTGCTCTTCCGATCTCACACCAACTCATCTTCAGCA"
     misc_feature    complement(3566..3585)
                     /label="ITX10-F6_Amp-Rv"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /note="sequence: ATAATACCGCGCCACATAGC"
     primer_bind     complement(3566..3585)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: Ampicillin resistance gene, reverse primer"
     primer          complement(3566..3585)
                     /label="oITX10_F6_Amp-Rv"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ataataccgcgccacatagc"
     primer          complement(3566..3585)
                     /label="Amp-Rv_PhosThio**"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /note="sequence: tacgaCCAATCCTGTCCCTAGTGGCCCCataataccgcgccacatagc"
     primer          complement(3566..3585)
                     /label="oITX15_D5_Amp-R_miseq"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /note="sequence: ACTGGAGTTCAGACGTGTGCTCTTCCGATCTCataataccgcgccacatagc"
ORIGIN
        1 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
       61 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
      121 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
      181 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
      241 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
      301 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
      361 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
      421 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
      481 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
      541 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
      601 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
      661 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
      721 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
      781 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
      841 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
      901 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
      961 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     1021 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     1081 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     1141 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     1201 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     1261 ccagcaacgc ggccttttta cggttcctgg ccttttgcTG CCTTAACCCT TGTGTTATGT
     1321 TTCGGGTCAA TTTGACCCGT TTCGATTTTT GAGTTGACCA AAGTGCAGGT TATTCTTATT
     1381 TTTTTTCCAT GAAATCTTGT GACTTTTCCG CATCTAGGGT CATGAACTTC TGAGTAAAAT
     1441 CTGGAGTCTT TGTATGaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca
     1501 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga
     1561 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     1621 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     1681 ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc
     1741 gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct
     1801 gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg
     1861 gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc
     1921 tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat
     1981 ccaagctgtg accggcgcct acgatatcgc caccatggcc agctccgagg atgtcatcaa
     2041 agagtttatg agatttaagg tcaagatgga gggaagcgtc aacggacacg agttcgagat
     2101 tgagggagaa ggagaaggcc ggccttacga gggcacacaa accgctaagc tcaaggtcac
     2161 aaaaggagga cccctcccct tctcctggga tattctgagc cctcagttcc agtacggaag
     2221 caaagcctat gttaaacacc ctgccgacat ccctgactat ctgaagctct ccttccctga
     2281 aggcttcaag tgggagagat tcatgaactt cgaggacgga ggcgtggtga cagtcacaca
     2341 agatagcacc ctccaggacg gagagtttat ttataaggtg aaactcagag gaaccaactt
     2401 cccctccgat ggccctgtca tgcaaaaaaa aacaatggga tgggaagcct ccaccgagag
     2461 aatgtatcct gaggatggcg ctctgaaagg cgaaattaaa atgagactga aactcaaaga
     2521 cggaggacac tacgatgccg aggtcaaaac aacctacaag gccaagaaac aagtgcagct
     2581 gcctggcgcc tacatgactg atattaaact cgacattatc agccataatg gggactacac
     2641 catcgtggaa caatatgaga gagctgaggg cagacatagc acaggcgctg gataagtcga
     2701 caatcaacct ctgattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     2761 ccttttacgc tatgtgGATA CGCTGCTTTA ATGCCTTTGT ATCATGCGTT AACTAAACTT
     2821 GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA
     2881 AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA
     2941 TGTCTGGAAT TGACTCAAAT GATGTCAATT AGTCTATCAG AAGCTATCTG GTCTCCCTTG
     3001 AGCTCTTTCA TAAGCGTGTA TGTAATCTAA AATAAAGTTC TGACTGGTTT GACTTCATTT
     3061 TTTGACTGTT TTCAGACGGA TTACACAATA CGGGTCAAAA TGACCCGCAA CATCATAAAT
     3121 GTAATTATTT CTCAACATAA TACAAGGGTT AAAGGAGCCT CGTGATACGC CTATTTTTAT
     3181 AGGTTAATGT CATGATAATA ATGGTTTCTT Agacgtcagg tggcactttt cggggaaatg
     3241 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3301 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3361 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3421 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3481 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3541 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3601 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3661 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc a
//