LOCUS       moxbfp_plasmid_addgene_ 2808 bp ds-DNA     circular     17-OCT-2025
DEFINITION  .
KEYWORDS    "creator:SnapGene License" "marker:AmpR"
FEATURES             Location/Qualifiers
     rep_origin      50..638
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             846..851
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="codon_start: 1 note: E. coli lac promoter with an ""up"" mutation transl_table: 1 translation: FT,LYASGS,YN"
     CDS             852..869
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="codon_start: 1 note: E. coli lac promoter with an ""up"" mutation transl_table: 1 translation: FT,LYASGS,YN"
     CDS             870..876
                     /label="lac UV5 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="codon_start: 1 note: E. coli lac promoter with an ""up"" mutation transl_table: 1 translation: FT,LYASGS,YN"
     protein_bind    884..900
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer          885..925
                     /label="seq_FWD"
                     /note="sequence: tgtgagcggataacaatttcacacaggaaacagctCATATG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    909..912
                     /label="RBS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    920..925
                     /label="NdeI"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    923..1642
                     /label="moxBFP2"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             923..1642
                     /label="moxBFP2"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKFISTTGKLPVPWPTLVTTLSHGVQVFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSKLSKDPNEKRDHMVLLEFRTAAGITLGMDELYK*"
     misc_feature    1643..1648
                     /label="KpnI"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1667..1686
                     /label="barcode"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1687..1711)
                     /label="seq_REV"
                     /note="sequence: cgaaaagtgccacctgACGTCgtgc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        1722..1826
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     CDS             1827..1895
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             1896..2687
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
ORIGIN
        1 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
       61 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
      121 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
      181 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
      241 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
      301 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
      361 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
      421 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
      481 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
      541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
      601 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
      661 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
      721 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      781 cgaccgagcg cagcgagtca gtgagcgagg aagcTCTAGA aaagcgggct ttaggcaccc
      841 caggctttac actttatgct tccggctcgt ataatgtgtg gaattgtgag cggataacaa
      901 tttcacacag gaaacagctC ATATGGTTAG CAAGGGAGAA GAATTGTTCA CGGGCGTAGT
      961 TCCAATTCTG GTGGAGCTGG ACGGTGACGT TAATGGGCAT AAGTTTTCGG TCCGTGGTGA
     1021 GGGCGAAGGT GATGCAACAA ACGGCAAGCT GACGCTTAAG TTCATCAGCA CTACCGGCAA
     1081 GCTTCCAGTG CCGTGGCCCA CCCTAGTAAC TACGCTGTCT CATGGAGTGC AGGTGTTCGC
     1141 CAGATATCCA GACCACATGA AACAGCATGA TTTTTTCAAA AGTGCGATGC CTGAAGGTTA
     1201 CGTGCAGGAA CGGACAATTT TCTTTAAGGA TGATGGTACG TACAAGACGC GTGCAGAGGT
     1261 CAAATTTGAA GGAGACACGC TTGTTAACCG CATTGAGCTC AAGGGTGTGG ATTTCAAAGA
     1321 GGATGGCAAC ATTTTAGGAC ATAAATTGGA GTACAACTTC AACTCGCACA ACATTTACAT
     1381 AATGGCTGTT AAGCAAAAGA ACGGCATAAA AGCGAACTTC AAGATCCGTC ATAATGTAGA
     1441 AGATGGCTCG GTCCAACTTG CGGACCACTA TCAGCAAAAC ACCCCTATTG GTGATGGTCC
     1501 AGTCTTATTA CCTGACAGCC ACTACTTAAG TACACAGTCA AAGCTGTCCA AGGACCCTAA
     1561 CGAAAAGCGT GATCACATGG TACTCCTGGA ATTTCGCACA GCCGCAGGTA TTACTCTCGG
     1621 CATGGACGAG CTATATAAGT AAGGTACCta aGTGTGGCTG CGGAACNNNN NNNNNNNNNN
     1681 NNNNNNgcac GACGTcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1741 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1801 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1861 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1921 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1981 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     2041 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     2101 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     2161 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     2221 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     2281 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     2341 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     2401 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     2461 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     2521 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2581 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2641 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     2701 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2761 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagt
//