LOCUS       Exported                9870 bp ds-DNA     circular SYN 14-FEB-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..9870
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        94..112
                     /label=T7 promoter
                     /note="label: T7 promoter note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=promoter"
     CDS             120..275
                     /label=lacZ-alpha (fragment)
                     /note="codon_start: 1 label: lacZ-alpha (fragment) note: 
                     pLannotate note: /database=snapgene note: /fragment=True 
                     note: /identity=100.0 note: /match_length=89.7 note: 
                     /other=CDS transl_table: 1 translation: 
                     SLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGE"
     rep_origin      280..708
                     /label=f1 ori
                     /note="direction: RIGHT label: f1 ori note: pLannotate 
                     note: /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=rep_origin"
     promoter        761..865
                     /label=AmpR promoter
                     /note="label: AmpR promoter note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=promoter"
     CDS             866..1726
                     /label=AmpR
                     /note="codon_start: 1 label: AmpR note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=99.8 note: /match_length=100.0 note: /other=CDS 
                     transl_table: 1 translation: 
                     MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFR
                     PEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVR
                     ELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERD
                     TTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAG
                     ERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     primer_bind     complement(1493..1524)
                     /label=111
     rep_origin      1897..2485
                     /label=ori
                     /note="direction: RIGHT label: ori note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=99.8 note: /match_length=100.0 note: 
                     /other=rep_origin"
     CDS             2668..2760
                     /label=lacI (fragment)
                     /note="codon_start: 1 label: lacI (fragment) note: 
                     pLannotate note: /database=snapgene note: /fragment=True 
                     note: /identity=100.0 note: /match_length=8.6 note: 
                     /other=CDS transl_table: 1 translation: 
                     APNTQTASPRALADSLMQLARQVSRLESGQ*"
     protein_bind    2773..2794
                     /label=CAP binding site
                     /note="label: CAP binding site note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=protein_bind"
     promoter        2809..2839
                     /label=lac promoter
                     /note="label: lac promoter note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=promoter"
     protein_bind    2847..2863
                     /label=lac operator
                     /note="label: lac operator note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=protein_bind"
     promoter        2908..2926
                     /label=T3 promoter
                     /note="label: T3 promoter note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=promoter"
     promoter        2954..3180
                     /label=RSV promoter
                     /note="label: RSV promoter note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=promoter"
     LTR             3181..3361
                     /label=5' LTR (truncated)
                     /note="label: 5' LTR (truncated) note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: /other=LTR"
     misc_feature    3408..3533
                     /label=HIV-1 Psi
                     /note="label: HIV-1 Psi note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=misc_feature"
     CDS             3517..3880
                     /label=HIV-1 gag (fragment)
                     /note="codon_start: 1 label: HIV-1 gag (fragment) note: 
                     pLannotate note: /database=snapgene note: /fragment=True 
                     note: /identity=97.3 note: /match_length=24.2 note: 
                     /other=CDS transl_table: 1 translation: 
                     MGARASVLSGGELDRDGKKFG*GQGERKNIN*NI*YGQAGS*NDSQLILAC*KHQKAVD
                     KYWDSYNHPFRQDQKNLDHYIIQ*QPSIVCIKG*R*KTPRKL*TR*RKSKTKVRPPHSK
                     RPL"
     misc_feature    4026..4259
                     /label=RRE
                     /note="label: RRE note: pLannotate note: /database=snapgene
                     note: /fragment=False note: /identity=100.0 note: 
                     /match_length=100.0 note: /other=misc_feature"
     CDS             4636..4736
                     /label=Rev (fragment)
                     /note="codon_start: 1 label: Rev (fragment) note: 
                     pLannotate note: /database=snapgene note: /fragment=True 
                     note: /identity=99.0 note: /match_length=28.8 note: 
                     /other=CDS transl_table: 1 translation: 
                     THLPTPRGPDRPEGIEEEGGERDRDRSIRLVNG"
     misc_feature    4755..4872
                     /label=cPPT/CTS
                     /note="label: cPPT/CTS note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=misc_feature"
     promoter        4894..5208
                     /label=tight TRE promoter
                     /note="label: tight TRE promoter note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=promoter"
     misc_feature    5213..5239
                     /label=MCS (fragment)
                     /note="label: MCS (fragment) note: pLannotate note: 
                     /database=snapgene note: /fragment=True note: 
                     /identity=100.0 note: /match_length=47.4 note: 
                     /other=misc_feature"
     protein_bind    5286..5310
                     /label=attB1
                     /note="label: attB1 note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=protein_bind"
     misc_feature    5339..5391
                     /label=MCS
                     /note="label: MCS note: pLannotate note: /database=snapgene
                     note: /fragment=False note: /identity=100.0 note: 
                     /match_length=100.0 note: /other=misc_feature"
     CDS             5433..5531
                     /codon_start=1
                     /label=P3
                     /translation="SPEDEIQQLEEEIAQLEQKNAALKEKNQALKYG"
     CDS             5532..5561
                     /codon_start=1
                     /label=myc tag
                     /translation="EQKLISEEDL"
     CDS             5562..5915
                     /codon_start=1
                     /label=nTEV
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQT"
     primer_bind     5562..5586
                     /label=176
     misc_feature    6030..6492
                     /label=IRES
                     /note="label: IRES note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=misc_feature"
     protein_bind    7250..7274
                     /label=attB2
                     /note="label: attB2 note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=protein_bind"
     misc_feature    7305..7893
                     /label=WPRE
                     /note="label: WPRE note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=misc_feature"
     promoter        7936..8435
                     /label=PGK promoter
                     /note="label: PGK promoter note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=promoter"
     CDS             8449..9252
                     /label=NeoR/KanR
                     /note="codon_start: 1 label: NeoR/KanR note: pLannotate 
                     note: /database=snapgene note: /fragment=False note: 
                     /identity=99.8 note: /match_length=100.0 note: /other=CDS 
                     transl_table: 1 translation: 
                     MGLAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGA
                     LNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSI
                     MADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARL
                     KARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELG
                     GEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             9261..9341
                     /label=nef (fragment)
                     /note="codon_start: 1 label: nef (fragment) note: 
                     pLannotate note: /database=swissprot note: /fragment=True 
                     note: /identity=81.5 note: /match_length=27.8 note: 
                     /other=CDS transl_table: 1 translation: 
                     VEGPVPLRPMTYKAAVDLSHFLKEKGG"
     LTR             9355..9575
                     /label=3' LTR (Delta-U3) (fragment)
                     /note="label: 3' LTR (Delta-U3) (fragment) note: pLannotate
                     note: /database=snapgene note: /fragment=True note: 
                     /identity=99.5 note: /match_length=94.4 note: /other=LTR"
     polyA_signal    9647..9768
                     /label=SV40 poly(A) signal
                     /note="label: SV40 poly(A) signal note: pLannotate note: 
                     /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=polyA_signal"
     rep_origin      9787..9808
                     /label=SV40 ori (fragment)
                     /note="direction: RIGHT label: SV40 ori (fragment) note: 
                     pLannotate note: /database=snapgene note: /fragment=True 
                     note: /identity=100.0 note: /match_length=16.2 note: 
                     /other=rep_origin"
     rep_origin      join(9808..9870,1..73)
                     /label=SV40 ori
                     /note="direction: RIGHT label: SV40 ori note: pLannotate 
                     note: /database=snapgene note: /fragment=False note: 
                     /identity=100.0 note: /match_length=100.0 note: 
                     /other=rep_origin"
ORIGIN
        1 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
       61 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
      121 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      181 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      241 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
      301 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      361 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      421 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      481 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      541 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      601 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
      661 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
      721 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      781 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      841 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      901 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      961 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1021 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1081 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1141 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1201 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1261 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1321 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1381 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1441 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1501 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1561 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1621 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1681 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1741 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1801 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1861 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1921 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1981 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2041 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2101 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2161 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2221 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2281 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2341 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2401 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2461 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2521 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2581 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2641 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2701 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2761 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     2821 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     2881 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     2941 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     3001 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     3061 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     3121 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     3181 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3241 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3301 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3361 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     3421 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     3481 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     3541 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     3601 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     3661 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     3721 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     3781 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     3841 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     3901 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3961 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     4021 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     4081 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     4141 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     4201 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     4261 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     4321 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     4381 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     4441 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     4501 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     4561 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     4621 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     4681 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     4741 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     4801 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     4861 attcaaaatt ttatcgatca cgagactagc ctcgagttta ctccctatca gtgatagaga
     4921 acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc
     4981 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     5041 ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac
     5101 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg
     5161 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattcga
     5221 gctcggtacc cggggatcca ccggttcgtc gactagtcca gtgtggtgga attctgcaga
     5281 tatcaacaag tttgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatct
     5341 cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc
     5401 caccgagctc ggatccagta cccttcacca tgagcccgga agacgagatt cagcagctgg
     5461 aggaggagat tgcacaactc gaacagaaaa acgctgcgct gaaggagaag aatcaagccc
     5521 tcaagtacgg tgagcaaaaa ctgatttccg aagaagacct cggcgagtca ctctttaaag
     5581 gtcctcggga ctacaatcct atatcaagta cgatatgcca cttgacgaat gagagcgatg
     5641 gacacacaac gtctttgtac gggataggct ttggcccgtt catcatcact aataaacatc
     5701 tctttcggcg caataacggg accttgctcg tccaaagcct gcatggggta tttaaagtta
     5761 aaaataccac taccttgcaa cagcacttga tcgatggccg ggacatgata attattagga
     5821 tgcccaaaga ttttcccccg tttcctcaaa agctgaaatt cagagaacca caacgggaag
     5881 aacggatctg cctggtcaca acgaattttc aaacatagcc cctctccctc ccccccccct
     5941 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     6001 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     6061 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     6121 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     6181 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     6241 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     6301 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     6361 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     6421 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
     6481 acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt tcaccggggt
     6541 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgagggg
     6601 cgagggcgag ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg
     6661 caagctgccc gtgccctggc ccaccctcgt gaccaccctg agccacggcg tgcagtgctt
     6721 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     6781 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc acctacaaga cccgcgccga
     6841 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggcg tcgacttcaa
     6901 ggaggacggc aacatcctgg ggcacaagct ggagtacaac ttcaacagcc acaacatcta
     6961 tatcatggcc gtcaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacgt
     7021 ggaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     7081 ccccgtgctg ctgcccgaca gccactacct gagcacccag tccgtgctga gcaaagaccc
     7141 caacgagaag cgcgatcaca tggtcctgct ggagttccgc accgccgccg ggatcactct
     7201 cggcatggac gagctgtaca agtaaagcgg ccgcactcga gatatctaga cccagctttc
     7261 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgacaatcaa cctctggatt
     7321 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     7381 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     7441 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     7501 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     7561 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     7621 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     7681 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     7741 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     7801 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7861 cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctgcagata tccggactag
     7921 tgatctaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     7981 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     8041 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     8101 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     8161 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     8221 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     8281 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     8341 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     8401 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagccaatat gggattggcc
     8461 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     8521 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     8581 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     8641 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     8701 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     8761 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     8821 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     8881 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     8941 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     9001 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     9061 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     9121 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     9181 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     9241 gagttcttct gaggggatcc gtcgagggcc cggtaccttt aagaccaatg acttacaagg
     9301 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggc tagctcactc
     9361 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctagttag accagatctg
     9421 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     9481 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9541 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     9601 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     9661 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9721 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     9781 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9841 tctccgcccc atggctgact aatttttttt
//
