LOCUS       Exported               10106 bp ds-DNA     circular SYN 01-AUG-2025
DEFINITION  pANC98c-1_midi.
ACCESSION   pANC98c-1_midi
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..10106
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             772..864
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    877..898
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        913..943
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    951..967
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             1621..1984
                     /codon_start=1
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.3"
                     /note="/match_length=24.2"
                     /note="/other=CDS"
                     /translation="MGARASVLSGGELDRDGKKFG*GQGERKNIN*NI*YGQAGS*NDS
                     QLILAC*KHQKAVDKYWDSYNHPFRQDQKNLDHYIIQ*QPSIVCIKG*R*KTPRKL*TR
                     *RKSKTKVRPPHSKRPL"
     misc_feature    2130..2363
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             2740..2840
                     /codon_start=1
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
                     /translation="THLPTPRGPDRPEGIEEEGGERDRDRSIRLVNG"
     misc_feature    2859..2976
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        2998..3312
                     /label=tight TRE promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    3317..3343
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=47.4"
                     /note="/other=misc_feature"
     protein_bind    3390..3414
                     /label=attB1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    3443..3495
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     primer_bind     complement(3514..3537)
                     /label=181
     CDS             3537..3602
                     /codon_start=1
                     /label=Feature 36
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3606..3653
                     /codon_start=1
                     /label=NES(nuclear export signal)
                     /translation="EELSQALASSFSVSSG"
     CDS             3657..3926
                     /codon_start=1
                     /label=frb
                     /translation="LEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKET
                     SFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             3990..4289
                     /codon_start=1
                     /label=TEV protease (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=42.4"
                     /note="/other=CDS"
                     /translation="KSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFI
                     VGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFM"
     primer_bind     4289..4306
                     /label=182
     misc_feature    4405..4867
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             4881..5600
                     /codon_start=1
                     /label=EBFP2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     protein_bind    complement(5625..5649)
                     /label=attB2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    5680..6268
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        6311..6657
                     /label=PGK promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=69.4"
                     /note="/other=promoter"
     promoter        6650..6775
                     /label=PGK promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=25.2"
                     /note="/other=promoter"
     CDS             6789..7592
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7613..7681
                     /codon_start=1
                     /label=nef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=91.3"
                     /note="/match_length=23.7"
                     /note="/other=CDS"
                     /translation="VPLRPMTYKAAVDLSHFLKEKGG"
     LTR             7695..7915
                     /label=3' LTR (Delta-U3) (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.5"
                     /note="/match_length=94.4"
                     /note="/other=LTR"
     polyA_signal    7987..8108
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      8148..8283
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        complement(8304..8322)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             8330..8485
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
                     /translation="SLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQ
                     LRSLNGE"
     rep_origin      8490..8918
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        8971..9075
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             9076..9936
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9703..9734
                     /label=110
     primer_bind     complement(9703..9734)
                     /label=111
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaaattcaa aattttatcg atcacgagac tagcctcgag
     3001 tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga
     3061 acgatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     3121 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     3181 ttatccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     3241 gtatgtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac
     3301 cgtcagatcg cctggagaat tcgagctcgg tacccgggga tccaccggtt cgtcgactag
     3361 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggctttaaag
     3421 gaaccaattc agtcgactgg atctcgagct caagcttcga attctgcagt cgacggtacc
     3481 gcgggcccgg gatccaccgg tcgccaccga gctcggatcc agtacccttc accatggact
     3541 acaaagacca cgacggcgat tacaaggatc atgacatcga ttacaaagac gatgacgata
     3601 agatggaaga gctgagccag gctctggcca gctcctttag cgtgtcctcc ggaatgcttg
     3661 agatgtggca tgaaggcctg gaagaggcat ctcgtttgta ctttggggaa aggaacgtga
     3721 aaggcatgtt tgaggtgctg gagcccttgc atgctatgat ggaacgggga ccccagactc
     3781 tgaaggaaac atcctttaat caggcctatg gtcgagattt aatggaggcc caagagtggt
     3841 gcaggaagta catgaaatca gggaatgtca aggacctcct ccaagcctgg gacctctatt
     3901 atcatgtgtt ccgacgaatc tcaaagacta gaggatccgg tggtggcggg tctggaggag
     3961 gaggtagtgg tggaggtggc tctggtacca agagcatgtc tagcatggtg tcagacacta
     4021 gttgcacatt cccttcatct gatggcatat tctggaagca ttggattcaa accaaggatg
     4081 ggcagtgtgg cagtccatta gtatcaacta gagatgggtt cattgttggt atacactcag
     4141 catcgaattt caccaacaca aacaattatt tcacaagcgt gccgaaaaac ttcatggaat
     4201 tgttgacaaa tcaggaggcg cagcagtggg ttagtggttg gcgattaaat gctgactcag
     4261 tattgtgggg gggccataaa gttttcatgt agcccctctc cctccccccc cccctaacgt
     4321 tactggccga agccgcttgg aataaggccg gtgtgcgttt gtctatatgt tattttccac
     4381 catattgccg tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag
     4441 cattcctagg ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa
     4501 ggaagcagtt cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag
     4561 gcagcggaac cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga
     4621 tacacctgca aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag
     4681 agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc
     4741 ccattgtatg ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag
     4801 gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg
     4861 atgataatat ggccacaacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     4921 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg aggggcgagg
     4981 gcgagggcga tgccaccaac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     5041 tgcccgtgcc ctggcccacc ctcgtgacca ccctgagcca cggcgtgcag tgcttcgccc
     5101 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     5161 tccaggagcg caccatcttc ttcaaggacg acggcaccta caagacccgc gccgaggtga
     5221 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcgtcgac ttcaaggagg
     5281 acggcaacat cctggggcac aagctggagt acaacttcaa cagccacaac atctatatca
     5341 tggccgtcaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacgtggagg
     5401 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     5461 tgctgctgcc cgacagccac tacctgagca cccagtccgt gctgagcaaa gaccccaacg
     5521 agaagcgcga tcacatggtc ctgctggagt tccgcaccgc cgccgggatc actctcggca
     5581 tggacgagct gtacaagtaa agcggccgca ctcgagatat ctagacccag ctttcttgta
     5641 caaagtggtt gatatccagc acagtggcgg ccgctcgaca atcaacctct ggattacaaa
     5701 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     5761 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     5821 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     5881 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     5941 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     6001 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     6061 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     6121 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     6181 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     6241 cggatctccc tttgggccgc ctccccgcct ggaattctgc agatatccgg actagtgatc
     6301 taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     6361 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     6421 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     6481 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     6541 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     6601 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagggg
     6661 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
     6721 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg
     6781 cagccaatat gggatcggcc attgaacaag atggattgca cgcaggttct ccggccgctt
     6841 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     6901 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     6961 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     7021 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     7081 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     7141 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     7201 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     7261 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     7321 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     7381 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     7441 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     7501 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     7561 ccttctatcg ccttcttgac gagttcttct gaggggatcc gtcgagggcc cggtaccttt
     7621 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     7681 actggaaggc tagctcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     7741 ctctagttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     7801 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     7861 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
     7921 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     7981 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8041 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8101 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     8161 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8221 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8281 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     8341 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     8401 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     8461 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     8521 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     8581 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     8641 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     8701 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     8761 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     8821 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     8881 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     8941 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     9001 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     9061 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     9121 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     9181 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     9241 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     9301 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     9361 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     9421 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     9481 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     9541 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     9601 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     9661 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     9721 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     9781 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     9841 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     9901 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     9961 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
    10021 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
    10081 cccgtagaaa agatcaaagg atcttc
//
