LOCUS       Exported                8177 bp ds-DNA     circular SYN 07-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(250..284)
                     /label=592
     primer_bind     262..284
                     /label=557
     primer_bind     complement(445..490)
                     /label=602
     primer_bind     462..495
                     /label=601
     rep_origin      917..1505
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             1688..1780
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    1793..1814
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1829..1859
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1867..1883
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1928..1946
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        1974..2200
                     /label=RSV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     LTR             2201..2381
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    2428..2553
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             2537..2900
                     /codon_start=1
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.3"
                     /note="/match_length=24.2"
                     /note="/other=CDS"
                     /translation="MGARASVLSGGELDRDGKKFG*GQGERKNIN*NI*YGQAGS*NDS
                     QLILAC*KHQKAVDKYWDSYNHPFRQDQKNLDHYIIQ*QPSIVCIKG*R*KTPRKL*TR
                     *RKSKTKVRPPHSKRPL"
     ncRNA           2995..3331
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=Rfam"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=ncRNA"
     CDS             3656..3756
                     /codon_start=1
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
                     /translation="THLPTPRGPDRPEGIEEEGGERDRDRSIRLVNG"
     misc_feature    3775..3892
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    3895..3914
                     /label=common
     primer_bind     3895..3914
                     /label=98_F_bef_prom
     primer_bind     complement(3895..3914)
                     /label=99_R_bef_prom
     promoter        3912..4157
                     /label=tight TRE promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=78.1"
                     /note="/other=promoter"
     misc_feature    4162..4188
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=47.4"
                     /note="/other=misc_feature"
     protein_bind    4235..4259
                     /label=attB1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    4288..4340
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     primer_bind     4358..4380
                     /label=70
     primer_bind     4358..4380
                     /label=100
     primer_bind     complement(4358..4380)
                     /label=101
     CDS             4378..5148
                     /codon_start=1
                     /label=Feature 32
                     /translation="MGESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITN
                     KHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREP
                     QREERICLVTTNFQTKSMSSMVSDTSCTFPSSDGTFWKHWIQTKDGQCGNPLVSTRDGF
                     IVGIHSASNFTNTNNYFASVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKP
                     EEPFQPVKEATQLMNGSGEGRGSLLTCGDVEENPG"
     CDS             4381..5088
                     /codon_start=1
                     /label=TEV protease
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="GESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNK
                     HLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQ
                     REERICLVTTNFQTKSMSSMVSDTSCTFPSSDGTFWKHWIQTKDGQCGNPLVSTRDGFI
                     VGIHSASNFTNTNNYFASVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPE
                     EPFQPVKEATQLMN"
     primer_bind     complement(5067..5088)
                     /label=109
     CDS             5152..5868
                     /codon_start=1
                     /label=EBFP2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=99.6"
                     /note="/other=CDS"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIKV
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLLE
                     FRTAAGITLGMDELYK"
     primer_bind     5866..5895
                     /label=36
     protein_bind    complement(5893..5917)
                     /label=attB2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    5948..6536
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             6599..6736
                     /codon_start=1
                     /label=nef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=80.4"
                     /note="/match_length=22.8"
                     /note="/other=CDS"
                     /translation="VPLRPMTYKAAVDLSHFLKEKGGLEGLAHSQRRQDLLFACTGSLW
                     L"
     LTR             6722..6902
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     polyA_signal    6974..7095
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      7135..7270
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.3"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        complement(7291..7309)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             7317..7472
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
                     /translation="SLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQ
                     LRSLNGE"
     rep_origin      7477..7905
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        7958..8062
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(8063..8177,1..746)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.7"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
       61 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
      121 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
      181 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
      241 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
      301 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
      361 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
      421 cgatgcctgt agcgatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
      481 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
      541 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
      601 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
      661 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
      721 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
      781 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
      841 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
      901 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      961 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     1021 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     1081 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     1141 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     1201 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     1261 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     1321 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     1381 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     1441 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     1501 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     1561 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     1621 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     1681 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1741 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     1801 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     1861 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     1921 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc
     1981 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     2041 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     2101 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     2161 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     2221 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     2281 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     2341 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     2401 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     2461 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     2521 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     2581 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     2641 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     2701 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     2761 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     2821 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     2881 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     2941 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     3001 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     3061 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     3121 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     3181 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcgagaat
     3241 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     3301 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     3361 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     3421 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     3481 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     3541 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     3601 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     3661 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     3721 agacagagac agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa
     3781 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     3841 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttatcgatca
     3901 cgagactagc ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc
     3961 ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgtatgt
     4021 cgagtttatc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
     4081 agaacgtatg tcgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
     4141 tgaaccgtca gatcgcctgg agaattcgag ctcggtaccc ggggatccac cggttcgtcg
     4201 actagtccag tgtggtggaa ttctgcagat atcaacaagt ttgtacaaaa aagcaggctt
     4261 taaaggaacc aattcagtcg actggatctc gagctcaagc ttcgaattct gcagtcgacg
     4321 gtaccgcggg cccgggatcc accggtcgcc accgagccgg atccagtacc cttcaccatg
     4381 ggagaaagct tgtttaaggg gccgcgtgat tacaacccga tatcgagcac catttgtcat
     4441 ttgacgaatg aatctgatgg gcacacaaca tcgttgtatg gtattggatt tggtcccttc
     4501 atcattacaa acaagcactt gtttagaaga aataatggaa cactgttggt ccaatcacta
     4561 catggtgtat tcaaggtcaa gaacaccacg actttgcaac aacacctcat tgatgggagg
     4621 gacatgataa ttattcgcat gcctaaggat ttcccaccat ttcctcaaaa gctgaaattt
     4681 agagagccac aaagggaaga gcgcatatgt cttgtgacaa ccaacttcca aactaagagc
     4741 atgtctagca tggtgtcaga cactagttgc acattccctt catctgatgg cacattctgg
     4801 aagcattgga ttcaaaccaa ggatgggcag tgtggcaatc cattagtatc aactagagat
     4861 gggttcattg ttggtataca ctcagcatcg aatttcacca acacaaacaa ttatttcgca
     4921 agcgtgccga aaaacttcat ggaattgttg acaaatcagg aggcgcagca gtgggttagt
     4981 ggttggcgat taaatgctga ctcagtattg tgggggggcc ataaagtttt catggtgaaa
     5041 cctgaagagc cttttcagcc agttaaggaa gcgactcaac tcatgaatgg atccggcgag
     5101 ggtagaggca gcctgctcac ctgcggagac gtggaagaga atcctggccc agtgagcaag
     5161 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     5221 ggccacaagt tcagcgtgag gggcgagggc gagggcgatg ccaccaacgg caagctgacc
     5281 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     5341 ctgagccacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
     5401 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     5461 ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     5521 gagctgaagg gcgtcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     5581 aacttcaaca gccacaacat ctatatcatg gccgtcaagc agaagaacgg catcaaggtg
     5641 aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc agctcgccga ccactaccag
     5701 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acagccacta cctgagcacc
     5761 cagtccgtgc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     5821 cgcaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcact
     5881 cgagatatct agacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc
     5941 gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6001 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6061 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6121 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6181 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6241 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6301 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     6361 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6421 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6481 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     6541 aattctgcag atatccggac tagtgatcta attctaccgg ggatccgtcg agggcccggt
     6601 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     6661 ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac
     6721 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     6781 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     6841 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     6901 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     6961 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7021 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7081 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     7141 cccctaactc agcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7201 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     7261 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     7321 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     7381 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     7441 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     7501 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     7561 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     7621 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     7681 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     7741 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7801 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7861 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     7921 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     7981 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     8041 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     8101 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     8161 tgctgaagat cagttgg
//
