LOCUS       Exported               10052 bp ds-DNA     circular SYN 22-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Oct 21, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..10052
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        94..112
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             120..275
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=89.7"
                     /note="/other=CDS"
                     /translation="SLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQ
                     LRSLNGE"
     rep_origin      280..708
                     /direction=RIGHT
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        761..865
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             866..1726
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1897..2485
                     /direction=RIGHT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             2668..2760
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    2773..2794
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        2809..2839
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    2847..2863
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        2908..2926
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     promoter        2954..3180
                     /label=RSV promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     LTR             3181..3361
                     /label=5' LTR (truncated)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=LTR"
     misc_feature    3408..3533
                     /label=HIV-1 Psi
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             3517..3880
                     /codon_start=1
                     /label=HIV-1 gag (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.3"
                     /note="/match_length=24.2"
                     /note="/other=CDS"
                     /translation="MGARASVLSGGELDRDGKKFG*GQGERKNIN*NI*YGQAGS*NDS
                     QLILAC*KHQKAVDKYWDSYNHPFRQDQKNLDHYIIQ*QPSIVCIKG*R*KTPRKL*TR
                     *RKSKTKVRPPHSKRPL"
     misc_feature    4026..4259
                     /label=RRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             4636..4736
                     /codon_start=1
                     /label=Rev (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.0"
                     /note="/match_length=28.8"
                     /note="/other=CDS"
                     /translation="THLPTPRGPDRPEGIEEEGGERDRDRSIRLVNG"
     misc_feature    4755..4872
                     /label=cPPT/CTS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        4894..5208
                     /label=tight TRE promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     misc_feature    5213..5239
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=47.4"
                     /note="/other=misc_feature"
     protein_bind    5286..5310
                     /label=attB1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    5339..5391
                     /label=MCS
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             5430..6143
                     /codon_start=1
                     /label=POLG_TVMV (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=96.0"
                     /note="/match_length=8.2"
                     /note="/other=CDS"
                     /translation="MGSSKALLKGVRDFNPISACVCLLENSSDGHSERLFGIGFGPYII
                     ANQHLFRRNNGELTIKTMHGEFKVKNSTQLQMKPVEGRDIIVIKMAKDFPPFPQKLKFR
                     QPTIKDRVCMVSTNFQQKSVSSLVSESSHIVHKEDTSFWQHWITTKDGQCGSPLVSIID
                     GNILGIHSLTHTTNGSNYFVEFPEKFVATYLDAADGWCKNWKFNADKISWGSFTLVEDA
                     PEDDFMAKKTVAAIMD"
     misc_feature    6262..6724
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     CDS             6738..7439
                     /codon_start=1
                     /label=dTomato
                     /note="pLannotate"
                     /note="/database=fpbase"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLYGMDELYK"
     protein_bind    7467..7491
                     /label=attB2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     misc_feature    7522..8110
                     /label=WPRE
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        8153..8499
                     /label=PGK promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=69.4"
                     /note="/other=promoter"
     promoter        8492..8617
                     /label=PGK promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=25.2"
                     /note="/other=promoter"
     CDS             8631..9434
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9443..9523
                     /codon_start=1
                     /label=nef (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=81.5"
                     /note="/match_length=27.8"
                     /note="/other=CDS"
                     /translation="VEGPVPLRPMTYKAAVDLSHFLKEKGG"
     LTR             9537..9757
                     /label=3' LTR (Delta-U3) (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.5"
                     /note="/match_length=94.4"
                     /note="/other=LTR"
     polyA_signal    9829..9950
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      9969..9990
                     /direction=RIGHT
                     /label=SV40 ori (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=16.2"
                     /note="/other=rep_origin"
     rep_origin      join(9990..10052,1..73)
                     /direction=RIGHT
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
ORIGIN
        1 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
       61 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
      121 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      181 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      241 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
      301 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
      361 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
      421 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
      481 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
      541 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
      601 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
      661 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
      721 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
      781 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
      841 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
      901 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
      961 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     1021 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     1081 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     1141 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     1201 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     1261 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     1321 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     1381 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     1441 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     1501 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     1561 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     1621 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     1681 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     1741 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     1801 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     1861 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     1921 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     1981 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     2041 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     2101 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     2161 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     2221 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     2281 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     2341 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     2401 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     2461 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     2521 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     2581 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     2641 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     2701 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     2761 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     2821 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     2881 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     2941 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     3001 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     3061 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     3121 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     3181 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     3241 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3301 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3361 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     3421 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     3481 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     3541 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     3601 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     3661 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     3721 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     3781 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     3841 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     3901 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     3961 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     4021 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     4081 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     4141 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     4201 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     4261 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     4321 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     4381 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     4441 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     4501 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     4561 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     4621 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     4681 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     4741 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     4801 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     4861 attcaaaatt ttatcgatca cgagactagc ctcgagttta ctccctatca gtgatagaga
     4921 acgtatgtcg agtttactcc ctatcagtga tagagaacga tgtcgagttt actccctatc
     4981 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt
     5041 ttactcccta tcagtgatag agaacgtatg tcgagtttat ccctatcagt gatagagaac
     5101 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg
     5161 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagaattcga
     5221 gctcggtacc cggggatcca ccggttcgtc gactagtcca gtgtggtgga attctgcaga
     5281 tatcaacaag tttgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatct
     5341 cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggtcgc
     5401 caccgagctc ggatccagta cccttcacca tgggatcctc aaaagcactc ctgaagggag
     5461 tccgcgactt taatcctata tcagcgtgtg tatgtttgct tgaaaactcc tctgatgggc
     5521 attcagaaag gctttttggt attgggtttg gtccctacat aatagcaaac caacacctgt
     5581 ttcgacgcaa taacggcgag cttacgatta agacgatgca cggggaattt aaagtgaaaa
     5641 acagcacaca attgcaaatg aaacccgtcg aagggagaga catcattgta ataaagatgg
     5701 caaaggattt cccacccttt ccgcagaaat tgaaattccg gcaacctact ataaaggatc
     5761 gagtttgtat ggtttctacg aactttcagc aaaaatcagt ctcctccctc gtgtccgaat
     5821 cctctcacat cgttcacaag gaagatacca gtttctggca gcattggatt accacaaaag
     5881 acgggcagtg cggctcaccg ctggtgtcta tcattgacgg gaacattctg ggaatccact
     5941 ccctgacaca caccactaac ggctcaaact atttcgttga gttcccagag aagttcgtag
     6001 ctacatatct ggacgctgcg gacggctggt gcaagaactg gaaattcaac gcggacaaga
     6061 tttcatgggg tagtttcacg cttgtcgaag acgcgcccga ggacgacttt atggcgaaaa
     6121 aaaccgtggc tgctattatg gatagctagc ccctctccct cccccccccc ctaacgttac
     6181 tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat
     6241 attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat
     6301 tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga
     6361 agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca
     6421 gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac
     6481 acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt
     6541 caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca
     6601 ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt
     6661 aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
     6721 ataatatggc cacaaccatg gtgagcaagg gcgaggaggt catcaaagag ttcatgcgct
     6781 tcaaggtgcg catggagggc tccatgaacg gccacgagtt cgagatcgag ggcgagggcg
     6841 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggcggccccc
     6901 tgcccttcgc ctgggacatc ctgtcccccc agttcatgta cggctccaag gcgtacgtga
     6961 agcaccccgc cgacatcccc gattacaaga agctgtcctt ccccgagggc ttcaagtggg
     7021 agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt gacccaggac tcctccctgc
     7081 aggacggcac gctgatctac aaggtgaaga tgcgcggcac caacttcccc cccgacggcc
     7141 ccgtaatgca gaagaagacc atgggctggg aggcctccac cgagcgcctg tacccccgcg
     7201 acggcgtgct gaagggcgag atccaccagg ccctgaagct gaaggacggc ggccactacc
     7261 tggtggagtt caagaccatc tacatggcca agaagcccgt gcaactgccc ggctactact
     7321 acgtggacac caagctggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     7381 acgagcgctc cgagggccgc caccacctgt tcctgtacgg catggacgag ctgtacaagt
     7441 agagcggccg cactcgagat atctagaccc agctttcttg tacaaagtgg ttgatatcca
     7501 gcacagtggc ggccgctcga caatcaacct ctggattaca aaatttgtga aagattgact
     7561 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7621 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7681 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7741 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7801 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7861 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     7921 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7981 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     8041 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     8101 gcctccccgc ctggaattct gcagatatcc ggactagtga tctaattcta ccgggtaggg
     8161 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     8221 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     8281 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     8341 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     8401 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     8461 ggccaatagc agctttgctc cttcgctttc tgggctcagg ggcgggctca ggggcggggc
     8521 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     8581 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagccaat atgggatcgg
     8641 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
     8701 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     8761 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     8821 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     8881 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     8941 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     9001 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     9061 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     9121 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     9181 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     9241 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     9301 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     9361 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     9421 acgagttctt ctgaggggat ccgtcgaggg cccggtacct ttaagaccaa tgacttacaa
     9481 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag gctagctcac
     9541 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctagtt agaccagatc
     9601 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     9661 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     9721 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     9781 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     9841 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9901 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     9961 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
    10021 attctccgcc ccatggctga ctaatttttt tt
//
